• Analytical Science and Technology
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• v.22 no.6
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• pp.514-518
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• 2009

Coenzyme $Q_{10}$($CoQ_{10}$), a vitamin E-like substance, represents a components of the complex antioxidant system of the human organism. $CoQ_{10}$ levels in human plasma were determined by high performance liquid chromatography (HPLC) with UV detection. It was dissociated from lipoproteins by methanol and extracted into n-hexane with liquid-liquid extraction procedure, after centrifugation, the supernatant was dried under nitrogen gas stream. The residue was dissolved in the absolute ethanol. Determination of $CoQ_{10}$ was performed on a $C_{18}$ reversed-phase analytical column with ultraviolet detection at 275 nm and the mobile phase containing 15% (v/v) ethanol in methanol at a flow rate of 1.7 mL/min. The low limit of quantitation was 0.02 mg/L (S/N=10), the linearity between the concentration and peak height is from 0.1 to 2.0 mg/L. Twenty-four randomly selected plasma samples from apparently healthy, 27 to 44 year old individuals (males and females) were analyzed for total $CoQ_{10}$. The average level in these subjects was $0.62{\pm}0.13mg/L$ with the range of 0.41-0.98 mg/L. This method has a specific and a sufficient limit of quantitation (LOQ) for analysis of $CoQ_{10}$ in human plasma in both a clinical study and research at laboratories.

• The Journal of Korean Academic Society of Nursing Education
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• v.29 no.2
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• pp.124-137
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• 2023

Purpose: This study's aim was to confirm the importance and performance of the clinical practice of nurses working in the general wards of a tertiary hospital and to analyze their educational needs. Methods: The study employed a descriptive research design, and a self-reported questionnaire was developed and used for nurses in a tertiary hospital. Data were collected from July 22 to July 29, 2022, analyzed by an independent t-test, paired t-test, and one-way ANOVA for the importance and performance of each clinical nursing practice according to general characteristics, and then a post hoc verification was performed by Scheffé's test. An Importance-Performance Analysis and Borich needs assessment model were used to analyze clinical nursing education needs. Results: Clinical nursing practice performance showed a significant difference according to length of total clinical career, time working in current department, and preceptor experience. According to the results of the Importance-Performance Analysis, neurological evaluation, and nursing intervention, artificial respirator and high-flow oxygen inhalation nursing, chemotherapy, emergency nursing, and cardiopulmonary resuscitation were identified as belonging to the "concentrate here" quadrant. All these items ranked in the top 10 in the Borich needs assessment model. Conclusion: Based on these results, the current education system should be reviewed, and short and long term education strategies based on educational needs should be established to strengthen the competence of nurses.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.3B
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• pp.315-321
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• 2008

Recently, in the light of environments and utilization, countermeasures to preserve beaches in coastal area are required without depending on such as jetties and breakwaters. The necessity of integrated sand management including not only coastal sediment but also sediment discharge from hinterland rivers is increased so as to establish long-term counterplan for sediment transport. In this regard, the following subjects are examined in this study; efficient ways for discharged sand to be transported from a river to the neighboring coast, the river terrace occurrence and its growth at the river delta, measures to improve storage efficiency of the discharged sand and measures to prevent the sand resources from being discharged into the deep sea during flooding. In recent, A jetty of 260 m length was constructed at Namdae River mouth in the year of 2005 as a countermeasure against the occurrence of sand-bar at river mouth and its close. In this study, a series of numerical experiments were carried out to investigate the characteristics of sediment transport and morphological change due to the construction of jetty at the entrance of Namdae River mouth. Firstly, The sand discharge from Namdae River is quantified by one-dimensional numerical analysis assuming the mixed sand of three different particle diameters. Then, in order to understand the transport behavior of the sand discharge from river and river mouth phenomena the numerical experiments were then conducted to examine the flow behaviors of river efflux and wind generated circulations in coastal area. And, after establishing the numerical model system, which predicts the sea bed changes obtained from the flux model combining with the wave propagation, wave-induced currents and sediment transport models, the sediment transport in the vicinity of Namdae River mouth is analyzed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.2B
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• pp.225-231
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• 2006

The nonpoint source control is based on TPLMS (Total Pollution Load Management System) program. Recently, the Ministry of Environment in Korea has programed TPLMS for 4 major large rivers to improve the water quality in rivers by controling the total pollutant loadings from the watershed area. Usually the urbanization is the main pollutant sources, particularly for nonpoint pollutants, because of high imperviousness and high pollutant mass emissions. The stormwater runoff from urban areas is containing various pollutants such as sediments, metals and toxic chemicals due to human and vehicle activities. Of the various landuses, the highways are highly polluted landuses because of high pollutant accumulation rate by vehicle activities during dry periods. Therefore, this research is achieved to provide pollutant EMCs (Event Mean Concentrations) and mass loadings washed-off from highways during rainfall periods. Five monitoring locations were equipped with an automatic rainfall gage and an flow meter. The results show that the EMC ranges for 95% confidence intervals in highway land use are 45.52-125.76 mg/L for TSS, 52.04-95.48 mg/L for COD, 1.77-4.48 mg/L for TN, 0.29-0.54 mg/L for TP. The ranges of washed- off mass loading are $712.7-2,418.4mg/m^2$ for TSS and $684.1-1,779.6mg/m^2$ for COD.

• 한국노년학
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• v.31 no.4
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• pp.939-967
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• 2011

This study observed elderly women's adaptation process on separation by death in rural areas through grounded theory approach and developed its entity theory. Participants for this study were 14 elderly women who have lost their husbands in the last 12 months. The study was conducted for 8 months starting January 2010. Each interview per session took 40 to 90 minutes, study notes were taken on site, and recorded contents were transcribed by the researcher which was myself. The research data were collected by in-depth interview and with help of local community's nurses who were in charge of taking care of the participants. The collected data were analyzed by applying Strauss & Corbin's grounded theory (1998). As a result of study, 80 concepts, 28 subcategories, and 12 categories were deducted during open coding process. Adaptation process on separation by death was process of 'finding a way to live alone' which used strategy of 'attempting a make changes in life', and 'embracing the situation' which were influenced by conciliatory conditions of 'degree on economic condition', 'change in health', and 'supporting system' which focuses on phenomenon of 'bearing life alone' which is influenced by context condition of 'marital chemistry of couples during lifetime', and 'the couple's leadership during lifetime' together with casual conditions of 'the fall of wall'. The adaptation process accordance to time flow were divided into 4 steps which were step of 'shock and release of emotions', step of 'longing and resentment', step of 'resignation and acceptance', and step of 'life's restructure'. Above results suggest right directions for welfare for the aged and process of 'finding a way to live alone' for participants by controlling several factors and using intervention strategy, and provided basic data required for developing and applying practical welfare mediation.

• Korean Journal of Heritage: History & Science
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• v.56 no.3
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• pp.254-273
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• 2023

In 2023, the Cultural Heritage Administration of Korea is transforming a system that has been in existence for the past 60 years. In these circumstances, an increasing recognition of the need for such changes is intended to start the study of the historical context in the conceptual development of cultural heritage. The employment of imported concepts of heritage created the demand for understanding at least the original contexts. Many European concepts have been introduced. In this study, the French Revolution is selected as the starting point for historical research on conceptual development. France opened a new horizon to national heritage since the establishment of the Republic at the end of the 18th century. The French Revolution placed monuments denied by the collapse of the Ancien Regime back into the boundaries of protection. In this process, the Commission des Monuments and the Commission Temporaire des Arts were created. There were limits to conservation activities in the context of the revolution and war. However, it is meaningful in that they established conservation principles with instructions and created new value for looking at monuments. It was pioneering in that it demonstrated the perspective of national heritage. This is significant because the top flow of conceptual development has led to a monument historique, bien culturel, and patrimoine culturel in France. This history provides a universal essence and has great implications for Korea as a divided country

• Journal of Environmental Impact Assessment
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• v.32 no.6
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• pp.488-502
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• 2023

Ecosystem service accounting must measure ecosystem supply functions, demand, and the actual service flows that occur between them. In order to measure flows, supply and demand relationships must be defined, and a methodology that can objectify complex connections is needed. Although various studies on ecosystem services have been conducted in Korea, but researches on accounting for ecosystem services are not enough. The purpose of this study is to evaluate flood control ecosystem services by applying the EU methodology studied in the Experimental Ecosystem Account (EEA) of System of Environmental Economy Account (SEEA) and explore ways to introduce ecosystem account. To conduct the study, the ecosystem's runoff retention potential, social and economic demand for flood control, and actual service benefit flows formed from the relationships between them were modeled and quantified on a spatial basis. As a result of calculating the actual flow of flood control ecosystem services, the total domestic service amount was calculated to be 165,595 (ha), and it was confirmed that much of it was concentrated in agricultural land. In order to account for domestic flood control services in the future, key spatial data such as land cover maps must be continuously established and managed, and researches on input data and methodologies applicable to various spatial scopes such as national, regional, and unit watersheds are expected to be necessary.

• Journal of The Korean Association For Science Education
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• v.27 no.9
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• pp.907-918
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• 2007

This study was conducted to explore key factors of expertise development of talented scientists who achieved outstanding research performance according to the stages of expertise development and dimensions of individual-domain-field. To fulfill the research purpose, 31 domestic scientists who were awarded major prizes in the field of science were interviewed in-depth from March to September, 2007. Stages of expertise development were analyzed in light of Csikszentmihalyi's IDFI (individual-domain-field interaction) model. Self-directed learning, multiple interests and finding strength, academic and liberal home environment, and meaningful encounter were major factors affecting expertise development in the exploration stage. In the beginner stage, independence, basic knowledge on major, and thirst for knowledge at university affected expertise development. Task commitment, finding flow, finding their field of interest and lifelong research topic, and mentor in formal education were the affecting factors in the competent stage. Finally, placing priority, communication skills, pioneering new domain, expansion of the domain, and evaluation and support system affected talented scientists' expertise development in the leading stage. The meaning of major patterns of expertise development were analyzed and described. Based on these analyses, educational implications for nurturing scientists were suggested.

• IMMUNE NETWORK
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• v.23 no.5
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• pp.39.1-39.15
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• 2023

Coronavirus disease 2019 (COVID-19) vaccination may non-specifically alter the host immune system. This study aimed to evaluate the effect of COVID-19 vaccination on hepatitis B surface Ag (HBsAg) titer and host immunity in chronic hepatitis B (CHB) patients. Consecutive 2,797 CHB patients who had serial HBsAg measurements during antiviral treatment were included in this study. Changes in the HBsAg levels after COVID-19 vaccination were analyzed. The dynamics of NK cells following COVID-19 vaccination were also examined using serial blood samples collected prospectively from 25 healthy volunteers. Vaccinated CHB patients (n=2,329) had significantly lower HBsAg levels 1-30 days post-vaccination compared to baseline (median, -21.4 IU/ml from baseline), but the levels reverted to baseline by 91-180 days (median, -3.8 IU/ml). The velocity of the HBsAg decline was transiently accelerated within 30 days after vaccination (median velocity: -0.06, -0.39, and -0.04 log₁₀ IU/ml/year in pre-vaccination period, days 1-30, and days 31-90, respectively). In contrast, unvaccinated patients (n=468) had no change in HBsAg levels. Flow cytometric analysis showed that the frequency of NK cells expressing NKG2A, an NK inhibitory receptor, significantly decreased within 7 days after the first dose of COVID-19 vaccine (median, -13.1% from baseline; p<0.001). The decrease in the frequency of NKG2A⁺ NK cells was observed in the CD56^dimCD16⁺ NK cell population regardless of type of COVID-19 vaccine. COVID-19 vaccination leads to a rapid, transient decline in HBsAg titer and a decrease in the frequency of NKG2A⁺ NK cells.

• Knowledge Management Research
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• v.24 no.4
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• pp.103-129
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• 2023

This study aims to analyze the network structure of technological innovation resources in SMEs, especially manufacturing firms, and reveal the differences between innovative and non-innovative firms. The study first analyzes connection centrality, flow-mediated centrality, and power centrality for all firms, and derives structural equivalence through CONCOR analysis. Then, the network structure of innovative and non-innovative firms was compared and analyzed according to innovation performance and creation. The results show that entrepreneurship and corporate innovation strategy have a significant impact on the analysis of technological innovation resources of all firms. According to the CONCOR analysis, the innovation resources of SMEs are organized into seven clusters, which can be defined as intrinsic product innovation resources, competitive advantage promotion resources, cooperative activities resources, information system resources, and innovation protection resources. The network analysis of innovative and non-innovative firms showed that innovative firms focused on enhancing competitiveness and improving quality, while non-innovative firms tended to focus more on existing products and customers. In addition, innovative firms had eight clusters, while non-innovative firms had six clusters, suggesting that innovative firms utilize resources diversely to pursue structural change and new value creation, while non-innovative firms operate technological innovation resources in a more stable form. This study emphasizes the importance of entrepreneurship and corporate innovation strategy in SMEs' technological innovation, and suggests that strong internal efforts are needed to increase innovativeness. These findings have important implications for strategy formulation and policy development for technological innovation in SMEs.