• 분석과학
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• 제37권2호
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• pp.98-113
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• 2024

The current investigation entails the characterization of five degradation products (DPs) formed under different stress conditions of loteprednol using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, this study developed a stable high-performance liquid chromatography (HPLC) method for evaluating loteprednol along with impurities. The method conditions were meticulously fine-tuned which involved the exploration of the appropriate solvent, pH, flow of the mobile phase, columns, and wavelength. The method conditions were carefully chosen to successfully resolve the impurities of loteprednol and were employed in subsequent validation procedures. The stability profile of loteprednol was exposed to stress degradation experiments conducted under five conditions, and DPs were structurally characterized by employing LC-MS/MS. The chromatographic resolution of loteprednol and its impurities along with DPs was effectively achieved using a Phenomenex Luna 250 mm C18 column using 0.1 % phosphoric acid, methanol, and acetonitrile in 45:25:30 (v/v) pumped isocratically at 0.8 mL/min with 243 nm wavelength. The method produces an accurate fit calibration curve in 50-300 ㎍/mL for loteprednol and LOQ (0.05 ㎍/mL) - 0.30 ㎍/mL for its impurities with acceptable precision, accuracy, and recovery. The stress-induced degradation study revealed the degradation of loteprednol under basic, acidic, and photolytic conditions, resulting in the formation of seven distinct DPs. The efficacy of this method was validated through LC-MS/MS, which allowed for the verification of the chemical structures of the newly generated DPs of loteprednol. This method was appropriate for assessing the impurities of loteprednol and can also be appropriate for structural and quantitative assessment of its degradation products.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 추계학술대회 논문집
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• pp.293-296
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• 2001

The dynamic softening mechanisms of AISI 316, AISI 304 and AISI 430 stainless steels were studied with torsion test in the temperature range of $900 - 1200^{\circ}C$ and the strain rate range of $5.0x10^{-2}-5.0x10^0/sec$. The austenitic stainless steels, such as AISI 316 and AISI 304 were softened by dynamic recrystallization (DRX) during hot deformation. Also, the evolutions of flow stress and microstructure of AISI 430 ferritic stainless steel show the characteristics of continuous dynamic recrystallization (CDRX). To establish the quantitative equations for DRX of AISI 316 stainless steel, the evolution of flow stress curve with strain was analyzed. The critical strain (${\varepsilon}_c$) and strain for maximum softening rate (${\varepsilon}^{*}$) could be confirmed by the analysis of work hardening rate ($d{\sigma}/d{\varepsilon}={\theta}$). The volume fraction of dynamic recrystallization ($X_{DRX}$) as a function of processing variables, such as strain rate ( $\varepsilon$ ), temperature (T), and strain ( $\varepsilon$ ) were established using the ${\epsilon}_c$ and ${\varepsilon}^{*}$. For the exact prediction the ${\varepsilon}_c,\;{\varepsilon}^{*}$ and Avrami' exponent (m') were quantitatively expressed by dimensionless parameter, Z/A, respectively. It was found that the calculated results were agreed with the experimental data for the steels at my deformation conditions. Also, we can reasonably conclude that the DRX, CDRX and grain refinement of stainless steels can be achieved by large strain deformation at high Z parameter condition.

• Computers and Concrete
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• 제22권2호
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• pp.227-237
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• 2018

Today, the modeling of concrete as a material within finite element simulations is predominantly done through nonlinear material models of concrete. In current sophisticated computational systems, there are a number of complex concrete material models which are based on theory of plasticity, damage mechanics, linear or nonlinear fracture mechanics or combinations of those theories. These models often include very complex constitutive relations which are suitable for the modeling of practically any continuum mechanics tasks. However, the usability of these models is very often limited by their parameters, whose values must be defined for the proper realization of appropriate constitutive relations. Determination of the material parameter values is very complicated in most material models. This is mainly due to the non-physical nature of most parameters, and also the large number of them that are frequently involved. In such cases, the designer cannot make practical use of the models without having to employ the complex inverse parameter identification process. In continuum mechanics, however, there are also constitutive relations that require the definition of a relatively small number of parameters which are predominantly of a physical nature and which describe the behavior of concrete very well within a particular task. This paper presents an example of such constitutive relations which have the potential for implementation and application in finite element systems. Specifically, constitutive relations for modeling the plane stress state of concrete are presented and subsequently tested and evaluated in this paper. The relations are based on the incremental theory of elastic strain-hardening plasticity in which a non-associated flow rule is used. The calculation result for the case of concrete under uniaxial compression is compared with the experimental data for the purpose of the validation of the constitutive relations used.

• 대한기계학회논문집
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• 제11권3호
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• pp.454-464
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• 1987

본 연구에서는 충격하중하에서 고변형도 .epsilon.=ln(h/h$_{o}$ )＞1.0, 고변형도율 (.epsilon.＞$10^{3}$m/s/m)로 변형하는 재료에 대하여 응력, 변형도, 변형도율사이의 함수관 계를 유도하고, 다음과 같은 현상들을 규명하였다. (1) 고변형도율에서 응력, 변형 도, 변형도율사이의 함수관계식 유도. (2) 압축하중시 시편과 접촉부재사이의 접촉면 에서 발생하는 마찰영향의 조사. (3) 유동응력과 시편의 기하학적 형상사이의 관계식 유도. (4) 압축하중시 재료의 제동현상(lock-up phenomena)의 해석.

• 콘크리트학회논문집
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• 제25권2호
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• pp.241-250
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• 2013

건설폐기물의 증가에 따라 지속가능한 기술로 폐콘크리트의 재사용에 대한 연구가 활발해지고 있다. 따라서 이 연구에서는 순환골재를 활용한 콘크리트의 유동성, 압축강도 및 쪼갬인장강도에 대한 특성을 조사하였다. 실험변수로는 콘크리트의 설계강도, 순환골재의 함수상태, 총 굵은골재량에 대한 순환굵은골재의 치환율, 플라이애쉬 사용 유무 등을 사용하여 11가지 경우에 대한 시험체 제작과 실험을 계획하였다. 굳지 않은 순환골재 콘크리트의 유동성을 평가하기 위해 ICAR 레오미터를 이용하여 유동곡선실험을 실시하였고, 슬럼프 실험 결과와 비교하였다. 순환골재와 플라이애쉬의 사용은 슬럼프와 유동곡선 모두 콘크리트의 유동성을 향상시키는 결과를 얻었다. 순환골재의 치환율이 증가할수록 항복응력이 감소하였으며, 이를 통해서 슬럼프와 항복응력은 대략적으로 반비례 관계에 있는 것을 확인하였다. 또한 플라이애쉬를 함유한 순환골재 콘크리트는 플라이애쉬를 함유하지 않은 경우에 비해 상당히 낮은 소성점도를 보였다. 순환골재를 사용한 콘크리트의 압축강도, 쪼갬인장강도는 치환율이 증가할수록 감소하는 경향을 보였고, 순환골재를 30% 이상 치환한 경우는 이러한 경향이 보다 뚜렷하게 보였다. 순환골재를 30% 치환하고, 플라이애쉬를 사용한 콘크리트는 장기강도 증진에 도움이 되는 것으로 판단된다.

• Structural Engineering and Mechanics
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• 제68권4호
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• pp.475-483
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• 2018

Square tubular columns are commonly used in moment resisting frames, while through-diaphragm connection is the most typical configuration detail to connect the H-shaped beam to the column. However, brittle fracture normally occurs at the complete joint penetration weld between the beam flange and the through-diaphragm due to the stress concentration caused by the geometrical discontinuity. Accordingly, three improved types of through-diaphragm are presented in this paper to provide smooth force flow path comparing to that of conventional connections. Tensile tests were conducted on four specimens and the results were analyzed in terms of failure modes, load-displacement response, yield and ultimate capacity, and initial stiffness. Furthermore, strain distributions on the through-diaphragm, the beam flange plate, and the column face were comprehensively evaluated and discussed. It was found that all the proposed three types of improved through-diaphragm connections were able to reduce the stress concentration in the welds between the beam flange and the through-diaphragm. Furthermore, the stress distribution in connection with longer tapered through-diaphragm was more uniform.

• 한국철도학회논문집
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• 제11권3호
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• pp.317-325
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• 2008

최근 연구결과 레일연마 및 장대레일화로 누적통과톤수에 의한 레일교체기준의 연장이 가능한 것으로 보고되었다. 본 연구에서는 현재 도시철도에서 사용 중인 대표 궤도구조에 대해 실운행 열차하중에 의한 궤도의 동적 응답을 분석하여 현 궤도의 상태평가를 하였으며 궤도의 상태 변화에 따른 레일의 부담력을 검토하였다. 또한, 측정된 응력파형을 Rainflow Count Method를 이용하여 응력히스토그램을 작성하고 등가응력을 상정하여 신규 레일 용접부의 S-N 선도에 적용함으로써 노후레일의 누적피로손상도 및 휨 피로수명을 산정하였다. 최종적으로 본 연구에서는 궤도구조 및 상태를 고려한 레일교체기준의 개정을 제안하고자 하였다.

• Animal Bioscience
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• 제36권7호
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• pp.1120-1129
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• 2023

Objective: This study aimed to determine the protective efficacy of Buddha's Temple (BT) extract against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in Gallus gallus chicken embryo fibroblast cell line (DF-1) and its effects on the cell lipid metabolism. Methods: In this experimental study, Gallus gallus DF-1 fibroblast cells were pretreated with BT 10^-7 for 24 hours, followed by their six-hour exposure to t-BHP (100 μM). Water-soluble tetrazolium salt-8 (WST-8) assays were performed, and the growth curve was computed. The intracellular gene expression changes caused by BT extract were confirmed through quantitative polymerase chain reaction (qPCR). Flow cytometry, oil red O staining experiment, and thin-layer chromatography were performed for the detection of intracellular metabolic mechanism changes. Results: The WST-8 assay results showed that the BT pretreatment of Gallus gallus DF-1 fibroblast cell increased their cell survival rate by 1.08%±0.04%, decreased the reactive oxygen species (ROS) level by 0.93%±0.12% even after exposure to oxidants, and stabilized mitochondrial activity by 1.37%±0.36%. In addition, qPCR results confirmed that the gene expression levels of tumor necrosis factor α (TNFα), TIR domain-containing adapter inducing IFN-beta (TICAM1), and glucose-regulated protein 78 (GRP78) were regulated, which contributed to cell stabilization. Thin-layer chromatography and oil red O analyses showed a clear decrease in the contents of lipid metabolites such as triacylglycerol and free fatty acids. Conclusion: In this study, we confirmed that the examined BT extract exerted selective protective effects on Gallus gallus DF-1 fibroblast cells against cell damage caused by t-BHP, which is a strong oxidative inducer. Furthermore, we established that this extract significantly reduced the intracellular ROS accumulation due to oxidative stress, which contributes to an increase in poultry production and higher incomes.

• 대한화학회지
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• 제23권4호
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• pp.217-236
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• 1979

소성변형에 대한 著者들의 이론을 초소성합금(Zn-Al eutectoid, A1-Cu, Pb-Sn, Sn-Bi, Mg-Al eutectics)에 적용하였다. 그 결과 초소성합금의 소성변형은 두 개의 grain boundary流動單位의 平行連結로 나타낼 수 있었다. 이 두 개의 流動單位는 流動式에 나타나는 parameter $X_{gj}/{\alpha}_{gj}$와 ${\beta}_{gj}$(j=1 혹온 2)로 表現할 수 있으며 이들을 實驗的으로 求할 수 있었다. 著者들의 流動式은 實驗과 잘 一致하였다. Strain rate sensitivity 對 -In(strain rate) 곡선을 이론으로 구한 결과 유동단위수만큼의 peak가 ${\beta}_{gj}$(j=1 or 2) 값에 따라 분리되어 나타났고 초소성의 조건도 ${beta}_{gj}$값에 의하여 결정됨을 알았다. ${\beta}_{gj}값의 粒子크기 依存性을 구하였고 온도변화에 따른 ${\beta}_{gj}$값 변화로부터 각 流動單位의 활성화엔탈피, ${\Delta}H_{gj}^{\neq}$도 구하였다. 그 결과 ${\Delta}H_{gj}^{\neq}$는 재료성분원소들의 grain boundary 자체확산에 의한 활성화엔탈피와 같이 나타났고 또 이들은 粒子 크기 증가에 마라 증가함을 보였다

• Geomechanics and Engineering
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• 제20권6호
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• pp.517-525
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• 2020

The long-term stability of rock engineering is significantly affected by the time-dependent deformation behavior of rock, which is an important mechanical property of rock for engineering design. Although the hard rocks show small creep deformation, it cannot be ignored under high-stress condition during deep excavation. The inner mechanism of creep is complicated, therefore, it is necessary to investigate the relationship between microscopic creep mechanism and the macro creep behavior of rock. Microscopic numerical modeling of sandstone creep was performed in the investigation. A numerical sandstone sample was generated and Parallel Bond contact and Burger's contact model were assigned to the contacts between particles in DEM simulation. Sensitivity analysis of the microscopic creep parameters was conducted to explore how microscopic parameters affect the macroscopic creep deformation. The results show that the microscopic creep parameters have linear correlations with the corresponding macroscopic creep parameters, whereas the friction coefficient shows power function with peak strength and Young's modulus, respectively. Moreover, the microscopic parameters were calibrated. The creep modeling curve is in good agreement with the verification test result. Finally, the creep curves under one-step loading and multi-step loading were compared. This investigation can act as a helpful reference for modeling rock creep behavior from a microscopic mechanism perspective.