• Journal of Aquaculture
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• v.20 no.3
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• pp.160-167
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• 2007

Embryonic and pre-leptocephalic larvae development of the eel, Anguilla japonica, are described following natural fertilization in the indoor tank of $23^{\circ}C$ water temperature. Following a routine hormone treatment technique for the brood stock, female eels were artificially matured by weekly intramuscular injections of salmon pituitary extracts (SPE) at a dosage of 20 mg/kg body weight (BW) for a total of 10-14 doses to induce ovarian maturation, while male eels received weekly intramuscular injections of human chorionic gonadotropin (HCG) at a dosage of 1 IU/g BW for a total of 6-10 doses to induce testicular maturation in a separate aquarium and induced natural spawning. Fertilized eggs of about 1.0 mm in diameter were pelagic and showed a typical discoidal cleavage. Hatching occurs 38 hrs after fertilization at a water temperature of $23^{\circ}C$. The newly hatched larvae measured about 3.0 mm in total length and the number of myomeres averages 42. Their mouths and anuses were opened at 4.5 days and the yolk sacs of the pre-leptocephalic larvae were almost absorbed at 6.5 days after hatching. Pre-leptocephalic larvae survive for 14.5 days. At this time they are $5.87{\pm}0.25mm$ in total length and have about 98 myomeres. However, morphological characterization of embryonic and pre-leptocephalic larvae were not different between natural fertilization and artificial fertilization by the dry method.

• Korean Journal of Ichthyology
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• v.20 no.4
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• pp.272-278
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• 2008

Optimum concentration of alizarine complexone (ALC) for staining of otoliths for Pacific cod was determined by examination of stained quality, growth and survival rates. Small juveniles ($34.1{\pm}1.8mm$ TL, $mean{\pm}SD$) were immersed in four different concentrations (20, 40, 60, 80 mg/L) and large ones ($73.8{\pm}1.2mm$ TL, $mean{\pm}SD$) in five different concentrations (1, 5, 10, 20, 40 mg/L) of ALC for 24 hrs. The fish were then reared and observed for 14 days. The stained quality of otoliths was evaluated by dividing them into four grades by the presence of fluorescent stains on the otoliths under G-excited UV light. The small juveniles stained with the different ALC concentrations had 100% staining success, and their growth rate and body weight did not differ significantly between treatments. Optimal ALC concentration for the smaller juveniles was 20 mg/L in the present experiment. On the other hand, distinct fluorescent rings were confirmed on otoliths of larger juveniles in the 10 mg/L treatment with high survival rate. These results suggest that ALC staining at concentrations of 10 mg/L with larger juveniles will be helpful for release-recapture experiments for stock enhancement of Pacific cod.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.44 no.4
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• pp.282-293
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• 2008

In order to develop the substitutive materials for natural baits of swimming crab pots, the attracting effects of swimming crab such as the preference of baits which were made of the by-products of marine and stock raising through the water tank experiments and fishing experiments. On the investigation of mean entrapped catch number to the pot by the baits after putting the 4 kinds of baits, mackerel(M), mackerel with grinded mackerel s internals($MM_I$), mackerel with tuna s internals$MM_I$) and makerel with grinded krill(MK) each in one pot by turns, $MM_I$ and MK were entrapped mean 3.9(13.0%) and they were a little more comparing to M, and $MT_I$ is least with mean 2.1(7.0%)(F=12.913, P < 0.05). Otherwise, on the preference investigation of swimming crabs by the baits after putting the 4 kinds of baits in the 4 pots each, M was entrapped mean 3.0(10%), but $MM_I$, $MT_I$ and MK were mean 1.2(4.0%), 1.0(3.3%) and 1.5(5.0%) each and they were only 30-50% of M(F=13.398, P < 0.05). On the preference investigation of swimming crabs by the 5 kinds of baits, mackerel(M), and krill(K), manila clam($M_C$), pig s fat($P_F$) and chicken s head($C_H$) which were used in substitutive baits, M was entrapped mean 3.2(10.7%), but K was about 50% of catch of M with mean 1.6(5.3%), and $M_C$, $P_F$ and $C_H$ were very few with mean 0.1-0.2(0.3-0.7%)(F=89.186, P < 0.05). On the preference investigation of swimming crabs by the pots which were put each the 3 kinds of baits, original krill(K), grinded krill with gluten and soybean oil cake($K_GGS$) and grinded krill with gluten, soybean oil cake and glycine($K_GGSG_L$) in the blue fluorescent hexahedral plastic bait cages(BF), and which were put the mackerel(M) in the non-fluorescent hexahedral red plastic bait cage($RF_N$), it was entrapped mean 3.0(10.0%) in the pot which was put the mackerel in the $RF_N$, and the same level in the pots which were put the K and $K_GGSG_L$ in the BF, but it was mean 2.0(6.7%) in the pots which was put the $K_GGS$ in BF and it was decreased by 30% of catch comparing to $RF_N$(F=3.750, P < 0.05). On the preference investigation of swimming crab by the pots which was put grinded tuna with gluten, soybean oil cake and glycine($T_IGSG_L$) in the blue fluorescent hexahedral plastic bait cage(BF), and which was put mackerel(M) in the nonfluorescent hexahedral red plastic bait cage($RF_N$), it was entrapped mean 3.3(11.0%) in the pot which was put mackerel in $RF_N$, and mean 2.7(9.0%) in the pot which was put $T_IGSG_L$ in BF and it was about 15% less comparing to use bait M(t=1.387, P < 0.05). As a results of fishing experiments, a plan for enhancing catching efficiency of $T_IGSG_L$ will be required because catching efficiency of $T_IGSG_L$, alternative bait, was half of fish catching efficiency of natural bait using mackerel. Fishing experiments were conducted 3 times using reinforced substitutive artificial bait that is reinforced attractive effect of $T_IGSG_L$ and composed of tuna intestine, grinded mackerel, gluten, soybean cake, glycine and alanine($T_IM_GGSG_LA$). Catching efficiency of $T_IM_GGSG_LA$ was about 80% of that of natural bait made of mackerel.