• 한국식품영양학회지
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• 제30권1호
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• pp.129-138
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• 2017

The purpose of this study was to evaluate the satisfaction with school meal service and the food preference in elementary school students who are in their growth period. A total of 484 students (242 boys) participated and completed a questionnaire survey. The results indicated that the merit of school meals was the highest in 'balanced diet'. 'Food hygiene' and 'delicious food' were the most important factors in school meals. Most of the students wanted 'balance between eastern and western foods' and 'new dishes' for the menu of school meals. For the distribution of meals, 'various kinds of side dishes' and 'warmth of dishes' were mainly required. The main problems of the current environment of school meals were 'long waiting time' and 'noise of the cafeteria'. In satisfaction with the school meal service, the highest satisfactory factor was 'staffs' cleanliness', following 'arrangement of furniture in cafeteria' and 'nutrition information-providing'; whereas, the lowest factor was 'staffs' kindness'. In the preference of foods, students preferred 'white rice'; whereas they did not like 'bean rice'; and 'fried rice' was preferred. In side dishes with meat and fish, most of the meats including 'Tangsuyuk' and 'Bulgogi' were preferred. For fish, 'fried hairtail' was preferred; whereas, 'fried Spanish mackerel' was not. In case of kimchi, 'Chinese cabbage kimchi' and 'cubed radish kimchi' were especially preferred. Considering these results, intensive improvement is required to increase school meal satisfaction by understanding the students' needs. An effort to allow the students' preferences to be reflected in the menu is also needed.

• 생명과학회지
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• 제28권9호
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• pp.1118-1126
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• 2018

본 논문은 양식 어류에게 많은 경제적 피해를 입히고 있는 다양한 어류질병세균 중에서 연쇄구균증에 대한 일반적인 특성과 종류, 진단 방법에 대하여 기존에 연구 보고된 논문을 기반으로 알아보고자 한다. 대표적인 어류연쇄구균증의 원인체로는 Lactococcus garvieae, Streptococcus iniae, S. parauberis가 있다. 연쇄구균증에 감염 시 나타나는 증상으로는 체색변화, 안구이상, 아가미 퇴색, 출혈, 복부팽만, 신장과 비장의 종대 등의 보이다 폐사가 일어난다. 또한 수온이 상승하는 6월부터 10월까지 주로 일어나며 집단적으로 발병하여 폐사가 일어난다. 현재 연쇄구균증을 진단하기 위한 기술로는 16S rRNA, 16S-23S rRNA intergenic spacer region (ISR), Random Amplified polymorphic DNA (RAPD), Ribotyion (RT), Loop-mediated isothermal amplification (LAMP) 등이 있다. 이중에서 현장에서 적용 가능성이 높은 LAMP법이 각광을 받고 있지만 결과 확인 등의 문제로 인하여 현재는 어려움을 겪고 있는 실정이다. 이에 현장에서 진단부터 결과 확인까지 손쉽게 사용할 수 있도록 문제점을 보완하면 연쇄구균증에 대한 경제적 손실을 최소화 할 것으로 사료된다.

• Journal of Radiation Protection and Research
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• 제26권2호
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• pp.101-111
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• 2001

본 연구는 염색체 1, 2, 4, 7, 8, 9 및 21번 염색체의 DNA probe를 이용하여 2Gy의 방사선을 조사한 후 DNA 양을 감안한 기대치와 관찰치의 차이를 비교함으로서 각 염색체의 방사선에 대한 감수성을 평가하여 궁극적으로 방사선 피폭시 생물학적 선량계로서 FISH기법의 타당성을 평가하고자 하였다. 1번 및 4번 염색체의 경우 상호전좌와 이동원 염색체의 관찰치가 기대치보다 더 높게 나타났으며 이와 반대로 2, 7, 8 및 9번 염색체의 경우 상호전좌와 이동원염색체의 관찰치 모두 기대치보다 낮게 나타났다. 2번 및 4번 염색체의 경우 1번 염색체보다 더 많은 acentric fragment의 빈도를 나타내었다. 1, 2, 및 4번 염색체 3종을 조합했을 때 상호전좌의 경우 관찰치와 기대치는 세포 100개당 25.5 및 25.40으로 차이가 없었으며 이동원염색체의 경우 13.25 및 13.2로 역시 거의 차이가 없게 나타났다. 따라서 본 연구결과 방사선 피폭시 발생하는 염색체이상 빈도는 염색체마다 DNA 양에 비례해 나타나지 않을 수 있어 각 염색체마다 방사선 감수성에 차이가 있을 것으로 판단된다. 또한 방사선 피폭시 생물학적 선량계로서 1, 2 및 4번을 동시에 관찰 할 경우 염색체 FISH 법을 활용하기 위하여 적절한 염색체 조합이라고 판단된다.

• 한국식품위생안전성학회지
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• 제30권1호
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• pp.92-97
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• 2015

본 연구에서는 포장방법 (진공, 일반)과 저장온도($4^{\circ}C$, $-20^{\circ}C$)를 달리한 육류 (소고기, 닭고기) 및 생선에 대해 저선량 감마선 반복조사를 적용하였을 때 나타나는 병원성 미생물 E. coli O157:H7 및 S. Typhimurium의 사멸효과를 확인하였다. 소고기와 닭고기의 경우 E. coli O157:H7은 최소 2 kGy 이상의 단일조사와 0.5 kGy 이상으로 반복조사 시 생육이 확인되지 않았고, S. Typhimurium은 최소 2 kGy 이상의 단일조사와 1 kGy 이상으로 반복조사 시 생육이 확인되지 않았다. 생선의 경우 최소 0.5 kGy 이상의 단일 및 반복조사 시 E. coli O157:H7 와 S. Typhimurium의 생육이 확인되지 않았다. 육류보다 생선이 낮은 선량으로 미생물을 사멸시킬 수 있었으며, 전반적으로 포장 방법에 따른 조사선량의 차이는 없었으나 저장온도의 경우 냉장보다 냉동의 경우가 좀 더 높은 조사선량을 요구하는 것으로 확인되었다.

• 생태와환경
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• 제41권3호
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• pp.301-310
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• 2008

본 연구는 2003$\sim$2006년까지 영산강 수계 22개 지점을 대상으로 어종들의 생태특성 및 지리적 분포양상을 분석하고 하구둑 축조 전.후 어류상의 변화를 비교 평가하였다. 본 연구에서 채집된 어류는 총 14과 44종으로 나타났고, 주요 우점종은 피라미, 큰납지리, 갈겨니, 붕어 등으로 나타났다. 생태지표특성 분석에 따르면, 내성종 및 잡식종의 상대풍부도는 각각 41%, 68%로 높게 나타나 화학적 수질악화 및 물리적 서식지 교란요인이 존재하는 것으로 사료되었다. 또한, 오염원의 영향을 받지 않은 지점(NPS)과 영향을 받은 지점(PS)의 비교 분석에 따르면, 종과 개체수가 최대 70%까지 차이를 보여 생태계 교란이 뚜렷하게 발생하였음을 시사하였다. 하천차수에 따른 어종 및 생태지표특성 분석에 따르면, 대조군지점(RS)은 하천차수가 높아질수록 총 종수 및 총 개체수가 증가하였고, 조사지점(SS)에서는 감소하는 경향을 보였다. 또한, 하천차수가 높아질수록 민감종과 충식성 어종의 상대풍부도는 감소하는 경향을 보였고 내성종과 잡식성 어종의 상대풍부도는 증가하는 것으로 나타났다. 외래어종이면서 육식종인 배스는 어종구성 및 영양단계구조에 변화를 가져와 궁극적으로 수생태계의 건강성을 악화시키는 요인으로 작용하게 되었다. 영산강 하구둑 건설은 정체수역을 증가시키고, 해수에서 담수화됨에 따라 정체수역을 선호하는 어종인 붕어, 잉어, 치리 등의 출현빈도가 높게 나타나게 되었으며, 기수역에 서식하는 어종과 회유성 어종의 출현빈도가 상대적으로 감소하게 되었다. 영산강 수계의 어류상은 외래어종의 도입, 물리적 서식환경의 교란과 오염원의 유입 등에 의하여 어종구성 및 영양단계구조가 변하였고, 궁극적으로 수생태 건강성에 영향을 미친 것으로 사료되었다. 이러한 변화를 예측 관리하기 위하여 장기적이고 지속적인 어류 모니터링이 필수적으로 요구되며, 외래어종에 의한 교란 억제와 물리적 서식지의 복원이 함께 진행되어야 할 것으로 사료되었다.

• Journal of Breast Disease
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• 제6권2호
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• pp.60-72
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• 2018

Purpose: According to American Society of Clinical Oncology/College of American Pathologists guidelines, breast cancer is human epidermal growth factor receptor 2 (HER2) positive if there is HER2 protein overexpression at a 3+ level on immunohistochemistry (IHC 3+) or gene amplification (more than six copies per nucleus) on fluorescence in situ hybridization (FISH+). However, there have been few reports on whether outcomes differ based on diagnosis by these two techniques. In this study, we compared outcomes based on the two methods in patients with HER2-positive breast cancer. Methods: This study was a retrospective analysis of HER2-positive breast cancer in 18,304 patients, including 14,652 IHC 3+ patients and 3,652 FISH+ patients from the Korean Breast Cancer Society Registry. We compared breast cancer-specific survival and overall survival based on IHC 3+ and FISH+ status with or without trastuzumab. Results: Breast cancer-specific survival was significantly different between the IHC 3+ and FISH+ groups, with 5-year cumulative survival rates of 95.0% for IHC 3+ and 98.5% for FISH+ patients who did not receive trastuzumab (p=0.001) in Kaplan-Meier methods. However, there were no significant differences in breast cancer-specific survival and overall survival between IHC 3+ and FISH+ groups regardless of trastuzumab treatment in Cox proportional hazards models. Conclusion: The survival outcomes were not affected by the different two diagnostic methods of HER2-positive breast cancer. Further research to evaluate differences in prognosis and other characteristics according to the diagnostic methods of HER2 positivity is needed in the future.

• 한국식품조리과학회지
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• 제30권2호
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• pp.170-182
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• 2014

The study surveyed the dietary behavior, food intake, and food preference for breakfast of elementary, middle, or high school students, a total of 354 boys and 305 girls. The students skipped breakfast due to no appetite, followed by no time. Elementary school students ate grains and potatoes more often than middle or high school students(p<0.05), and bap was the most frequently consumed cereals, with juk and tteok as the least. High school students ate meat, ham, sausage and boiled fish cake (eomuk) more often than the elementary or middle school students(p<0.05). Middle school students ate milk and dairy products as well as vegetables and fruits more often(p<0.05) than other foods. Preference for fish and fresh vegetables and salad was significantly high in middle school students. The score of dietary behavior was the highest in elementary school students, followed by middle and high school students(p<0.001). As the students advanced to the upper grade schools, breakfast skipping increased, with lowered dietary behavior scores. The results suggest that a variety of breakfast menus and recipes should be developed at home and schools by considering the preference of students in order to reduce breakfast skipping and to improve their satisfaction with breakfast.

• 생태와환경
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• 제41권4호
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• pp.441-448
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• 2008

The purpose of this study was to analyze chemical water quality, fish trophic guilds, tolerance indicators, and fish community conditions in the Gap Stream and to compare the stream conditions between the unimpacted site and impacted site. This study was conducted in the physically stable season (May 2008) to minimize physical impacts such as flow and hydrological disturbance, and applied the study in the Gap Stream with two sites of unimpacted upstream site (Unim-S), mainly surrounded by forested area and impacted site (Im-S), influenced by the wastewater disposal plants and industrial complex in the urban region. Chemical data analysis showed that the degree of organic matter pollution, based on BOD, and COD, was $2{\sim}3$ fold greater in the Im-S than the Unim-S, and that TP, as eutrophication indicators, was 4.7 fold greater in the Im-S. Also, $NH_3-N$ was in 8.2 fold greater in the Im-S ($6.25\;mg\;L^{-1}$) than the Unim-S ($0.76\;mg\;L^{-1}$), indicating a massive influence of wastewater from the disposal plant. Similar results were found in other chemical parameters. Thus, chemical impacts in the Im-S were evident, compared to the unimpacted site. Evaluations of tolerant indicator species indicated that sensitive species were dominant in the Unim-S (23.9%) and tolerant species were dominant (97.8%) in the Im-S. Condition factor (CF) was averaged 0.95 ($0.68{\sim}1.18$) in the Unim-S and 1.08 ($0.93{\sim}1.22$) in the Im-S. Fish community in the Unim-S and Im-S was categorized as Zacco-community and Hemibarbus-community, respectively, and the community diversity index (H') was significantly (p<0.05) higher in the Unim-S (0.810) than the Im-S (0.466). Overall, our results suggest that the comparison approach of various chemical and ecological indicators provide important information in identifying multiple stressors in the stream ecosystems.

• 한국환경복원기술학회지
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• 제22권6호
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• pp.125-138
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• 2019

This study aims to highlight the possibility in identifying species composition of fish communities using the environmental DNA (eDNA) metabarcoding technique, from both of the technical introduction and the pilot test at urban ecological streams. This new emerging survey technique using eDNA is getting popular in the world as a compensating way for the conventional field survey. However, the application to the domestic cases has yet to be studied. We attempted to use this technique for identifying fish species observed at four survey points in Hwangguji-chon, Suwon City. As a result, the detected number of species by eDNA sampled once in May was significantly matched with the total number of observed species in annual field surveys. Additionally eDNA results indicated the presence possibility of the unobserved species in field last year, even though the validation may be required. This survey technique seems to be more efficient and applicable to diverse situations of the fields and species, thereby needs to be studied further. We discussed the pros and cons of the application and summarized the research directions in future.

• 한국환경보건학회지
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• 제47권1호
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• pp.87-100
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• 2021

Objectives: The purpose of this study was to analyze the factors affecting Bisphenol A (BPA) exposure in children and adolescents using the results of the Korean National Environmental Health Survey (KoNEHS) cycle 3. Methods: A total of 2,380 subjects (n=571, 887, and 922 for 3-5, 6-11, and 12-17 years of age, respectively) were analyzed using an environmental exposure survey and environmental chemical substances concentration levels. Univariable linear regression analysis was performed to determine associated variables such as sex, age, income level, housing type, secondhand smoke time, cup noodles and canned food consumption, seafood consumption, new furniture (within the previous six months), drinking water type, and consumption of herbal medicines. Variables with p-values of less than 0.2 were extracted from the results and a multivariable linear regression analysis was performed using stepwise selection. Results: Univariable linear regression analysis showed positive associations between BPA concentration levels and variables including sex, age, secondhand smoke time, new furniture (within the previous six months), renovated living space (within the previous six months), fish and shellfish consumption, plastic-bottled drink consumption, and herbal medicine. As a result of performing multivariable linear regression analysis, the lower was the age the higher was the concentration of BPA levels. Additionally, women showed higher BPA levels than those of men. The more frequently fish was consumed, the higher was the BPA concentration. Moreover, higher BPA concentrations were observed when taking herbal medicine. Conclusions: The main factors affecting BPA concentration levels were age, gender, and consumption of fish and herbal medicine.