• 한국약용작물학회지
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• 제16권3호
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• pp.195-198
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• 2008

Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

• Toxicological Research
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• 제30권4호
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• pp.311-316
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• 2014

For the detection of trace copper to be used in medical diagnosis, a sensitive handmade carbon nanotube paste electrode (PE) was developed using voltammetry. Analytical optimized conditions were found at 0.05 V anodic peak current. In the same conditions, various common electrodes were compared using stripping voltammetry, and the PE was found to be more sharply sensitive than other common electrodes. At optimum conditions, the working ranges of $3{\sim}19{\mu}gL^{-1}$ were obtained. The relative standard deviation of $70.0{\mu}gL^{-1}$ was determined to be 0.117% (n = 15), and the detection limit (S/N) was found to be $0.6{\mu}gL^{-1}$ ($9.4{\times}10^{-9}M$). The results were applied in detecting copper traces in the kidney and the brain cells of fish.

• Journal of Advanced Marine Engineering and Technology
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• 제35권5호
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• pp.690-699
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• 2011

A numerical model analysis was performed to analyze the stability of the mooring lines of an automatic submersible fish cage system in waves and currents. The fish cage system consisted of a 12-angled rigid frame, net cage, cover net, 12 upper floats, 12 tanks(for fixed and variable ballast), mooring lines, anchors, and a control station. Simulations were performed with the cage at the surface of the water and at a depth of 20 m. A Morison equation type model was used for simulations of the system in two configurations. The force parameters described both regular and random waves, with and without currents, and their values were input to the model. Mooring tension calculations were conducted on the mooring lines, grid lines and lower bridle lines of the cage. The stability of the mooring lines was checked under both static and dynamic conditions.

• Fisheries and Aquatic Sciences
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• 제3권1호
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• pp.49-51
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• 2000

This study was performed to confirm the relationship between viral propagation and apoptosis by the infection of marine birnavirus strain (MABV NF-4) on chinook salmon embryo (CHSE-214) cells. After 6 hr viral infection, MABV was detected by PCR method. Also, as a result of DNA assay on the cells, MABV infection resulted in a typical feature of apoptosis, DNA fragmentation. The results suggest that MABV replicated to high concentrations during the early stage of infection induces apoptosis.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.91-95
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• 1992

Two experiments were conducted to evaluate the performance of broilers and layers fed locally produced fish waste meal (LFWM) in Western Samoa. LFWM, which was produced by mincing and oven drying of rejects from filleted fish, was included in both broiler and layer diets at the complete expense of imported meat and bone meal (MBM). The experiments were of a completely randomised design. In experiment 1, 120 day-old Strabro broiler chicks were raised to 49 days of ages. In experiment 2, 80 Shaver 288 pullets, 20 weeks of age, were housed in pairs in cages and raised up to 72 weeks of age. There were no differences (p > 0.05) in performance of broilers as measured in terms of final bodyweights, feed intake, feed efficiency and carcass yield between the two diets. Similarly, no differences (p > 0.05) existed in egg production, egg weight and feed required per kilogramme of eggs. It is concluded that LFWM is nutritionally as good as imported MBM and could be used to completely replace MBM in broiler and layer diets.

• Clinical and Experimental Reproductive Medicine
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• 제31권1호
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• pp.29-39
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• 2004

Objectives: This study was performed to evaluate the laboratory system for successful PGD using fluorescence in situ hybridization (FISH) and the clinical outcome of PGD cycles in five years experiences. Methods: A total of 181 PGD-FISH cycles of 106 couples were performed, and diagnosed chromosome normality in the preimplantation embryos. The laboratory and clinical data were classified by the following optimization steps, and statistically analyzed. Phase I: Blastomere biopsy with two kinds of pipettes, removal of cytoplasmic proteins without treatment of pepsin and culture of biopsied embryos with single medium; Phase II: Blatomere biopsy with single pipette, removal of cytoplasmic proteins with pepsin and culture of biopsied embryos with single medium; Phase III: Blastomere biopsy with single pipette, removal of cytoplasmic proteins with pepsin and culture of biopsied embryos with sequential media. Results: A total of 3, 209 oocytes were collected, and 83.8% (2, 212/2, 640) of fertilization rate was obtained by ICSI procedure. The successful blastomere biopsies were accomplished in 98.6% (2, 043/2, 071) of embryos, and the successful diagnosis rate of FISH was 94.7% (1, 935/ 2, 043) of blastomeres from overall data. Embryo transfers with normal embryos were conducted in 93.9% (170/181) of started cycles. There was no difference in the successful rate of biopsy and diagnosis among Phase I, II and III. However, the pregnancy rate per embryo transfer of Phase III (38.8%, 26/67) was significantly (p<0.05) higher than those of Phase I (13.9%, 5/36) and Phase II (14.9%, 10/67). Conclusions: The laboratory optimization and experience for the PGD with FISH procedure can increase the pregnancy rate to 38.8% in the human IVF-ET program. Our facility of PGD with FISH provides the great possibility to get a normal pregnancy for the concerned couples by chromosomal aberrations.

• 농촌의학ㆍ지역보건
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• 제26권2호
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• pp.161-179
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• 2001

성주(星州)댐(1994년(年) 담수(潭水) 시작(始作))이 건설(建設)되고, 가야산국민(伽倻山國民)호탤 등(等), 백운동(白雲洞) 집단시설지구(集團施設地區)가 들어선 이후(以後), 대가천(大駕川)에서 채집(採集)되는 담수어(淡水魚)에 있어서 흡충류피낭유충(吸蟲類被囊幼蟲) 기생상(寄生狀)의 변화(變化)를 알아보기 위해 1998년(年) 10월(月)부터 2000년 9월(月)까지 대가천(大駕川)의 상(上), 중(中), 하류(下流)5개처(個處)에서 투망(投網), 낚시, 사발모지 등(等)으로 담수어(淡水魚)를 채집(採集)하였다. 채집(採集)된 어류(魚類)는어종별(魚種別)로 분류(分類)한다음, 어체부위별(魚體部位別)로 흡충류피낭유충(吸蟲類被囊幼蟲)의 기생율(寄生率)을 조사(調査)함과 동시(同時)에 신선(新鮮)한 어육(魚肉)1g 당피낭유충수(當被囊幼蟲數)를 계산(計算)하여 감염도(感染度)를 추정(推定)하였다. 한편, 얻은 결과(結果)를 Lee(1993)의 조사성적(調査成績)과 비교(比較)하였다. 6과(科) 19종(種)의 어류(魚類)에서 8정(種)의 흡충류피낭유충(吸蟲類被囊幼蟲), 즉 간흡충(肝吸蟲), Centrocestus armatus, Cyathocotyle orientalis, Exorchis oviformis, Metagonimus sp ecies, Metacercaria hasegawai, Metorchis orientalis, Clinostomum complanatum, 및 소속미정(所屬未定) 흡충류(吸蟲類) 피낭유충(被囊幼蟲)A와 C를 검출(檢出)할 수 있었다. 대체(人體)에 기생(寄生)하는 간흡충(肝吸蟲)의 피낭유충(被囊幼蟲)은 7종(種)의 어류(魚類)에서 검출(檢出)할 수 있었고, 그 기생율(寄生率)은 1993년(年) Lee의 조사성적(調査成績)에 비(比)해 낮았으며, 기생도(寄生度)는 적었다. Metagonimus species 피낭유충(被囊幼蟲)은 1993년에서는 11종(種)의 어류(魚類)에서 검출(檢出)할 수 있었으며 그 율(率)은 최소(最小)1.2%, 최대(最大)33.3%의 감염율(感染率)을 나타내었는데 비(比)하여, 이번 조사(調査)에서는 6종(種)의 어류(魚類)에서 검출(檢出)할 수 있었으며, 그 율은 4.3- 20.0%이였다. 기생도(寄生度)는 1993年의 조사성적(調査成績)에 비(比)해 낮았다. Exorchis oviformis 유충(幼蟲)은 5종(種)의 어류(魚類)에서 검출(檢出)할 수 있었으며, Cyathocotyle orientalis는 7종(種), Metacercaria hasegawai는 9종(種), Centrocestus armatus는 3종(種), Clinostomum complonatum은 2종(種)의 어류(魚類)에서 검출(檢出)할 수 있었으며, 그 기생율(寄生率)과 기생도(寄生度)는 어종별(魚種別)로 큰 차이(差異)를 나타내었다. 비늘, 지느러미와 꼬리에 있어서 흡충류(吸蟲類) 피낭유충(被囊幼蟲)의 기생율(寄生率)은 너무 변동(變動)이 심(甚)하여 1993년(年)의 그 율(率)과 비교(比較)할 수 없었으나, 대체(大體)로 낮았다. 이상(以上)의 성적(成績)으로 보아 성주(星州)댐 건설(建設)과 가야산국민(伽倻山國民)호탤 등(等), 백운동(白雲洞) 집단시설지구(集團施設地區)가 들어선 이후(以後), 대가천(大駕川)에 서식(棲息)하고 있는 담수어(淡水魚)에 있어서 간흡충(肝吸蟲)을 위시(爲始)한 각종(各種) 흡충류(吸蟲類) 피낭유충(被囊幼蟲) 기생율(寄生率)과 기생도(寄生度)는 댐 담수(潭水)를 시작(始作)하기 전(前)(1994년)보다 낮음을 알았다.

• 수산해양기술연구
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• 제31권1호
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• pp.8-14
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• 1995

정치망 내외에 있어서 정어리 대형군의 행동조사는 1992년 1월 29일부터 2월 22일 사이에 일본국 석천현 칠미시 연안 정치망어장에서 소나를 이용하여 실시하였고, 소나의 영상기록을 해석하여 얻어진 결과를 요약하면 다음과 같다. 1. 정치망 바깥쪽 부근에서 이동중인 어군의 행동양식은 어군의 각 위치에 따라 다른 행동이 관찰되었고, 부분적인 확대와 축소를 보이면서 어군전체가 이동하였다. 2. 헛통입구로 들어간 어군은 등망방향, 헛통의 등방향, 헛통의 옆줄방향으로 분산되었으며, 그후 헛통의 등방향, 헛통의 옆줄방향, 입구방향의 어군은 등망쪽으로 조밀하게 이동하는 행동양식을 취하면서 입구로부터 외등망까지 이동하였다. 3. 정치망의 안쪽과 바깥쪽에 있어서 어군의 주연부가 확대하는 경우, 최대 이동속도는 바깥쪽에서 277cm/sec, 안쪽에서 176cm/sec이었다.

• Fisheries and Aquatic Sciences
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• 제7권4호
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• pp.192-203
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• 2004

The effects of temperatures, starvation, and kind of foods on growth, RNA/DNA ratio and protein contents during metamorphosis and early juvenile stage of olive flounder (Paralichthys olivaceus) were examined. During metamorphosis, warm-acclimated fish showed higher RNA and DNA content than those of the cold-acclimated fish, excepting H stage (28 DAH) at which the ratio was higher at cold temperature. RNA/DNA ratio during metamorphosis showed similar values at two temperatures tested. However, after 42 DAH warm-acclimated juveniles had higher DNA content compared with cold-acclimated fish, resulted in marked decreases in RNA/DNA ratios. Higher RNA content at H stage of cold-acclimated fish was consistent with an increase in protein content. Growth of fish rearing at warm temperature was higher than those of fish at cold temperature during all experiments. In starvation experiment, contents of DNA, RNA and protein significantly decreased. Even though there were no significant differences in total length (TL) and body weight between the live mysid-fed and artificial pellet-fed fish at 35 mm TL, both RNA/DNA and protein/DNA ratios of the former group was significantly higher than those of the latter due primarily to lower DNA content of the live mysid-fed group. The results from this study suggest that temperature, starvation and kind of foods should be considered when RNA/DNA ratio applied to assessing the cultured larval and juvenile fish condition.

• Ecology and Resilient Infrastructure
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• 제6권1호
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• pp.1-11
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• 2019

본 연구는 생태 물길 복원 조성에 따른 어류 군집종에 미치는 영향을 파악하기 위하여 물리 서식처 분석을 수행하였다. 대상 어종은 피라미, 쉬리, 참갈겨니, 돌고기, 줄납자루, 밀어, 묵납자루, 꾸구리, 가는돌고기로 선정하였으며, 총 9개 어종으로 달천 내 서식하는 어류 중 95%를 차지한다. 흐름 분석은 2차원 모형인 River2D 모형을 사용하였으며, 서식처 분석은 서식처 적합도 곡선을 이용하는 서식처 적합도 모형을 사용하였다. 생태 물길 복원은 하도 내 위치한 돌보 철거 및 여울-소 구조 조성과 하상고 및 하폭의 변화를 통하여 조성하였다. 그 결과 생태 물길 복원 조성을 통하여 최적의 환경 생태 유량 조건 ($Q=7.0m^3/s$)에서 총 9종 군집종의 가중가용면적을 약 16% 향상시키는 것으로 나타났다. 이를 통하여 생태 물길 복원 조성이 하천 내 서식하는 다양한 군집종에 대하여 매우 유용한 방법임을 확인할 수 있다.