• 한국수산과학회지
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• 제40권5호
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• pp.300-307
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• 2007

This study investigated the effects of dietary supplementation with hot water extracts (HE) of selfheal (Prunella vulgaris) and Lactobacillus rhamnosus culture fluid (LF) on the growth and non-specific immune responses of juvenile olive flounder. A total of 270 fish ($5.07{\pm}0.01g$, average $weight{\pm}SD$) were divided randomly into nine groups, and three groups were fed one of three isonitrogenous (51% crude protein) and isocaloric (17.6 MJ/kg) diets. The diets contained no supplement, 50 mL hot water extract, or 50 mL L. rhamnosus fluid (designated as diets CON, HE and LF, respectively). During the 8-week feeding trial, the growth, feed utilization and survival of the fish were not significantly affected by the experimental diets. There were no significant differences in the hematocrit and hemoglobin of fish fed each of the experimental diets. However, the serum alanine aminotransferase and aspartate aminotransferase activity were lower with the dietary supplements containing HE and LF. The alanine aminotransferase activity of fish fed the HE diet was significantly lower than that of fish fed the control diet. The hematocrit, hemoglobin, and nitroblue tetrazolium (NBT) activity were higher 3 hr after feeding than 24 hr afterwards. The NBT activity of fish fed the HE and LF diets were significantly higher than that of fish fed the control diet 3 hr after feeding. The findings suggest that dietary supplementation with HE and LF could enhance the nonspecific immune responses of juvenile olive flounder.

• 한국어류학회지
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• 제7권2호
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• pp.187-194
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• 1995

동면어류의 물질대사에 대한 학술적 기초자료를 얻을 목적으로 자연산 미꾸라지의 암컷과 수컷을 시료로 하여 시기별 (산란기 전 ; 5월, 산란기 후 ; 8월, 동면직전 ; 11월, 동면직후 ; 3월)근육성분의 조성과 육조직내 탄수화물, 단백질, 지방질 분포를 조사한 결과는 다음과 같다. 비만도(肥滿度)는 암,수 모두가 산란기후에 다소 높았고, 동면직후에 가장 낮았다. 수분함량은 산란기 후에 가장 적었으나 동면직후에 가장 높았고, 조단백질과 조지방은 산란기 후가 가장 높았으나 동면직후에 가장 낮아 이들 함량의 변화 pattern이 상보적(相補的) 경향을 나타내었다. 그리고 탄수화물은 동면직전이 다소 많으나 동면직후에는 감소된 경향이었다. 근육 조직의 PAS염색 결과, 산란기후 표피의 점액 부위와 피하조직의 근육세포에서 비교적 강한 양성 반응을 나타내었다. 또한 진피층의 두께가 동면직후와 산란기 전이 산란기 후와 동면 직전보다 얇게 나타났다. Sudan black B 염색결과, 지방 성분 염색반응이 암컷은 산란기 전에 근육 세포층에 비교적 넓게 분포된 경향이었고, 수컷은 주로 점액층과 표피층에 나타났다. 산란기 후는 주로 표피 및 피하조직에 나타났고 동면직전과 후는 조직 전체에 분포하였으나 그정도가 동면직후에 다소 적은 편이었다.

• Preventive Nutrition and Food Science
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• 제13권3호
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• pp.204-211
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• 2008

Fish protein hydrolysates (FPHs) with different degrees of hydrolysis by treatment with alcalase, pronase, flavourzyme and trypsin and isolated peptide were prepared from Hwangtae (yellow dried pollack, Theragra chalcogramma). Hwangtae protein hydrolysate was fractionated according to the molecular weight into six major types of APO1 (1.3 kDa), APO2 (1 kDa), APO3 (<1 kDa), APACE (<1 kDa), APG1 (70 kDa) and APG2 (70 kDa) isolated from the hydrolysate using consecutive chromatographic methods. Soluble peptide were produced from Hwangtae and evaluated for their nutritional and functional properties. Some functional properties of FPHs were assessed and compared with those of egg albumin or the soybean protein. APO2 had the highest nitrogen solubility value (94.2%), emulsion capacity and emulsion stability of the Alaska Pollack peptide ranged from 12.4 to 39.5 (mL of oil per 200 mg of protein) and 44.0% to 77.5%, respectively. Highest and lowest fat adsorption values were observed for APG1 (9.9 mL of oil per gram of protein) and APO3 (3.8 mL of oil per gram of protein), respectively.

• 한국양식학회지
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• 제18권1호
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• pp.26-30
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• 2005

본 연구는 부상 건조사료와 습사료의 효능을 비교함과 동시에 이들 사료의 적정 공급률(만복 및 제한 공급)을 조사하기 위해 수행되었다 평균 체중 27 g의 넙치를 각 수조마다 500 마리씩 2반복으로 수용하여 부상 건조사료(EP1과 EP2)와 넙치용 분말사료(우성 사료)와 냉동 전갱이를 1:3의 무게 비율(습중량 기준)로 혼합하여 성형한 moist pellet (MP)을 만복 및 만복의 $85\%$로 공급하면서 10주간 사육하였다. 생존율은 모든 실험구에서 $94\%$ 이상이었으며, 증중량은 EP2를 만복 공급한 실험구가 가장 높았지만, EP1을 만복 공급한 실험구와 MP를 만복 및 제한 공급한 실험구들과는 유의한 차이가 없었다(P>0.05). 사료효율은 EPI과 EP2를 제한으로 공급한 실험구가 가장 높은 결과를 보였으며(P<0.05),동일한 사료 공급률에 있어 MP를 공급한 실험구가 EP1과 EP2를 공급한 실험구에 비해 유의적으로 낮은 결과를 보였다(P<0.05). 일일사료섭취율은 만복 및 제한 공급구 각각에서 MP를 공급한 실험구가 EP1 및 EP2을 공급한 실험구보다 유의하게 높았다(P<0.05). 단백질효율은 만복 및 제한 공급에 관계없이 MP를 공급한 실험구가 EPI 및 EP2를 공급한 실험구보다 유의하게 낮았다(P<0.05). 에너지 축적효율은 사료종류 및 공급률에 영향을 받지 않았다(P>0.05). 전어체의 수분, 단백질 및 회분의 함량은 모든 실험구에서 유의적인 차이를 보이지 않았다. 반면에 지질의 함량은 EP2와 MP의 만복 공급구에서 가장 높았고, EP1의 만복 공급구에서 가장 낮은 값을 보였다. 본 실험의 결과로부터, 본 연구에 사용된 EP 사료들은 현재 양식현장에서 사용하고 있는 생사료를 대체할 수 있을 것으로 생각되며, 사료는 만복으로 공급되는 것이 좋을 것으로 판단된다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.245.2-245
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• 1996

No Abstract, See Full Text

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.45-45
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• 2001

Heterocyclic aromatic amines (HCAs) are established genotoxic human cancer risks, that display their activity also in a number of animal models and in vitro. They are formed during the trying or broiling of creatinine-containing foods, mainly fish or meat. We established that mixing soy protein with ground meat blocks the formation of HCAs. we also observed that coating the surface of meat with polyphenols green or from black tea gives a dose - related reduction of the formation of HCAs.(omitted)

• Journal of Microbiology and Biotechnology
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• 제4권3호
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• pp.176-182
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• 1994

The light-organ symbiont of pine cone fish, Vibrio fischeri, senses its presence in the host and responds to environmental changes by differentially expressing its symbiosis-related luminescence genes. The V. fischeri luminescence genes are activated by LuxR protein in the presence of an autoinducer. In an effort to elucidate the mechanism of regulation of luxR, a plasmid containing luxR was mutagenized in vitro with hydroxylamine and a luxR mutant plasmid was isolated by its ability to activate luminescence genes cloned in E. coli in the absence of the autoinducer. The specific base change identified by DNA sequencing was only single base transition at ＋78 from the transcriptional start of luxR. Based on a Western immunoblot analysis, the nucleotide change directed the synthesis of much higher level of LuxR protein without any amino acid substitutions. The results suggest that the region including the ＋78th base is presumably internal operator required for autorepression of luxR, and the increased cellular level of LuxR results in activation of luminescence genes by autoinducer independent fashion.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.51-57
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• 2003

A cDNA encoding a putative alcohol dehydrogenase (ADH) class III was cloned from the silkworm, Bombyx mono The full length cDNA is 1,385 nucleotides long and contains an open reading frame of 1,128 bp encoding 376 amino acid residues. The B. mon ADH III protein sequence was aligned with ADH III known from various organisms. Interestingly, the protein sequence of B. mon ADH III showed 87% and 85% identity to ADH III from marine fish Sparus aurata and Branchiostoma floridae, respectively, whereas rather low sequence identity (83%) to Drosophila melanogaster ADH III was observed. Northern blot analysis revealed that B. mon ADH III mRNA is expressed in all tissues from larva examined: fat body, midgut, epidermis, silk gland and ovary, with the highest level found in the fat body.

• Fisheries and Aquatic Sciences
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• 제4권1호
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• pp.32-38
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• 2001

Several molecular biological techniques have been used to detect virus rapidly and accurately, but these methods have limitations in the early stage of viral infection with very low concentration of virus. We compared the detection limits of IMS-PCR, Western blot and ELISA with infectious hematopoietic necrosis virus OHNV). Four antibodies, rabbit anti-IHNV polyclonal antibody, anti-IHNV nucleocapsid protein monoclonal antibody, anti-IHNV nucleocapsid protein polyclonal antibody, and anti-IHNV glycoprotein polyclonal antibody, were tested to find out the most effective antibody for each method. The detection limit with IMS- PCR was $2\times10^6$ pfu when the viral RNA was extracted before RT-PCR. In the western blot with rabbit anti­IHNV polyclonal antibody one pfu of virus could be detected. In ELISA, 10 pfu of virus particles were detected with the same antibody.

• Journal of Life Science
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• 제9권2호
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• pp.34-39
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• 1999

The postsynaptic density (PSD) is a cytoskeletal specialization that is involved in the regulation of synaptic signal transduction. Mainly due to the hydrophobic nature of the PSD proteins, characterization of this intriguing structure at the molecular level has been very intractable until early 1990s. However, recent development in protein microchemistry and molecular cloning techniques allowed identification and characterization of the PSD proteins. As expected, cytoskeletal proteins constitute major components of the PSD. Other major PSD proteins have been identified by protein sequencing, and their genes were used to fish out associating proteins by yeast two-hybrid system expanding our knowledge on the molecular structure of the PSD significantly. In this review, I summarize proteins that are so far identified focusing on the glutamatergic synapses.