• Nutrition Research and Practice
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• 제11권6호
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• pp.492-499
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• 2017

BACKGROUND/OBJECTIVES: This study aimed to investigate the association of dietary patterns with overweight risk and all-cause mortality in pediatric cancer patients. SUBJECTS/METHODS: Prospective cohort study was undertaken; 83 cancer patients admitted to the pediatric cancer ward at a university hospital in Seoul were included and followed for obesity and death over 24 months. Food consumption data were collected from patients using validated meal order sheets for breakfast, lunch, and dinner at the pediatric cancer ward over 3 days. Using principal component analysis, three dietary patterns were derived from 29 food groups. RESULTS: Eighteen deaths occurred among the patient cohort during the follow-up period. The "spicy & fried meat and fish" dietary pattern was positively associated with overweight risk at both baseline [odds ratio (OR) = 4.396, 95% confidence interval (CI) = 1.111-17.385, P for trend = 0.023] and after 6 months (OR = 4.088, 95% CI = 1.122-14.896, P for trend = 0.025) as well as all-cause mortality (hazard ratios = 5.124, 95% CI = 1.080-24.320, P for trend = 0.042), when comparing the highest and lowest tertiles after adjusting for covariates. The "fish, egg, meat, and fruits & vegetables" dietary pattern was associated with lower overweight risk after 24 months (OR = 0.157, 95% CI = 0.046-0.982, P for trend = 0.084). CONCLUSION: The results imply that dietary patterns might be associated with weight gain and premature death among pediatric cancer patients.

• 한국어병학회지
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• 제6권2호
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• pp.191-195
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• 1993

With the rapid progress in seed production techniques, aquaculture production of economically important species of marine fish has been accelerated in Japan. Howecer, mass mortalities due to viral infections as well as other microbial infections have often occurred during the seed production and grow-out stages. Among these diseases, four viral diseases have been known in cultured Japanese flounder (Paralichthys olivaceus) since around 1980. In this paper, viral diseases of cultured flounder in Japan are briefly reviewed, with special attention to two viral diseases. viral epidermal hyperplasia and rhabdovirus infection which are relatively important because of their frequent occurrence. Viral epidermal hyperplasia is characterized by fin opacity and associated with high mortality in larval flounder Electron microscopy of affected epidermal cells and transmission experiments with tissue filtrates demonstrated that the disease was caused by a herpesvirus but the agent has not been isolated in fish cell lines. On the other hand, rhabdovires infection occurrs in juvenile and production size fish with hemorrhage in the skeltal muscle and fins, congestion of the gonads, and ascites. A rhabdovirys was isolated in RTG-2 cells from the diseased flounder as a causative agent, which was designated hirame rhabdovirus (HRV) or Rahbdovirus olivaceus. HRV is serologically distinguishable from other known fish rhabdoviruses. Intensive researches on these viral diseases started in 1980th. but properties of the causative agents and infection mechanisms have not been fully investigated. This results in difficulty in controlling these diseases.

• 한국어병학회지
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• 제27권2호
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• pp.85-89
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• 2014

Water temperature is a key environmental factor controlling the epizootics of viral diseases in fish. High water temperature is associated with the rapid spread of rock bream iridovirus (RBIV) disease and with high mortality of RBIV infected fish. Although protection of fish against iridoviral disease by active immunization has been reported, little information is available concerning whether fish survived from an epizootic of iridoviral disease can naturally acquire resistance against the viral disease. In the present study, we have demonstrated that juvenile rock bream, which survived from a natural epizootic of RBIV, acquired resistance against recurrence or reinfection of RBIV, and this resistance was established during the subsequent low water temperature period. Furthermore, the possible involvement of the adaptive humoral immune response in the resistance of the juvenile rock bream was suggested by in vivo neutralization experiment.

• 한국양식학회지
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• 제10권4호
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• pp.439-447
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• 1997

청평내수면연구소에서 발생한 쏘가리 치어의 대량 폐사 원인을 조사하였다. 병어로부터 분리된 원인균은 생화학적 및 생리학적 특성에 의해 Aeromonas hydrophila (A. hydrophila)로 동정되었다. A. hydrophila을 쏘가리 치어(3-4 g)에 침지, 경구투여, 근육피하 주사 및 세균이 분비한 가용성 세포의 산물 주사 등 여러 가지로 감염 실험을 하였다. 그 결과 근육피하 주사 실험군에 있어서는 다른 감염 실험에서보다 높은 폐사율을 보였고, 가용성 세포외 산물도 고농도 ($8{\times}10^9$ cfu/ml)로 처리한 어류에서는 3-6시간 이내에 모두 폐사되었다. 이러한 결과는 쏘가리 치어가 A. hydrophila에 감수성이 높은 것을 보여주었다.

• 한국어병학회지
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• 제34권2호
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• pp.133-140
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• 2021

With the recent isolation of a new betanodavirus in shellfish, Korean Shellfish Nervous Necrosis Virus (KSNNV), it has also been identified the reassortant KSNNV of two RNA segments, in which one segment is KSNNV genotype but the other one is known genotype. In this study, we confirmed that the ressortant KSNNVs obtained in previous screening study of our laboratory for betanodaviruses in shellfish were KS/RGNNV and RG/KSNNV type by performing two consecutive multiplex RT-PCR on each RNA1 and RNA2 segment (R1- and R2-discriminative multiplex two-step RT-PCR, respectively) to determine the genotype of each segment based on the size of amplicon. In the pathogenicity analysis, none of the reassortants induced specific external symptoms or mortality of VNN, but viruses of 2 × 10⁴~10⁵ copies/mg or more were detected at 14 days after injection (10⁷ copies/fish) in brain tissues of 4 species except for crucian carp and common carp among the 6 species of juvenile fish used. In addition, the histopathological features of weak but distinct vacuole formation were also found in the brain of these infected fish, but no difference was found between the two reassortants KS/RGNNV-KG and RG/KSNNV-CM.

• 한국수산과학회지
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• 제52권5호
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• pp.539-546
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• 2019

The major cultured marine fishes in sea off the coast Gyeongsangnam-do Province, South Korea, were assessed and included 9.3% rockfish Sebastes schlegelii, 7.8% red seabream Pagrus major, and 2.1% rock bream Oplegnathus fasciatus. The number of insurance payments related to disease mortality in cultured fish in 2017 was fourfold that in 2016. Economic loss in aquaculture due to disease in cultured fish is high and represents an important inhibitory factor affecting marine fishery productivity. In 2018, diseases led to severe production losses in several aquaculture species: 40.0% in rockfish, 11.4% in olive flounder Paralichthys olivaceus, 10.0% in filefish Thamnaconus modestus, and 9.3% in red seabream. Fish-parasitic pathogens such as Microcotyle sebastis, Alella spp., and Dactylogyrus spp. enter mainly via the gills and skin surface. Among bacterial pathogens, Vibrio species were most common, with Vibrio harveyi being the dominant species causing infections in these fishes. The bacterium Lactococcus garvieae is thought to exhibit host specificity in fish. The fish species in the present study exhibited a higher tendency for infection by heterologous pathogens than by a single pathogen; therefore, it is necessary to devise new strategies for treating diseases in cultured fish.

• Fisheries and Aquatic Sciences
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• 제26권11호
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• pp.660-668
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• 2023

This study aimed to investigate the growth performance of far eastern catfish (Silurus asotus) on outdoor fish farms to obtain basic data for the domestic eastern catfish aquaculture industry. An outdoor fish farm was directly monitored from June 2018 to October 2019 to determine the farming conditions, growth performance, and water quality. The growth performance in 2017 was analyzed using data from the same fish farm. Three years of monitoring showed that the fish farm required approximately 5-6 months between stocking, harvesting, and selling an S. asotus batch. The growth parameters, namely, the weight gain rate (WGR), specific growth rate (SGR) for culture periods, SGR for feeding periods, and feed coefficient rate (FCR), were 4,664.7%, 1.27%, 2.43%, and 1.25 in 2017; 6,452.0%, 1.52%, 2.79%, and 1.42 in 2018; and 3,270.0%, 1.11%, 2.12%, and 1.38 in 2019, respectively. Moreover, the WGR was two-fold higher in 2018 than 2019, whereas the FCR was more effective in 2019 than 2018, presumably because of the stocking density. No mass mortality was observed during the water quality analysis. The results of this study provide basic data for the development of the catfish industry.

• 한국어병학회지
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• 제25권2호
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• pp.111-116
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• 2012

본 연구에서는 2006-2008년 남해안 일대의 해상가두리에서 사육중인 능성어에서 발생하는 viral nervous necrosis (VNN)의 발생양상을 조사하였다. VNN은 사육 수온이 $24-26^{\circ}C$ 범위인 8월부터 발생하기 시작하여 수온이 $20-25^{\circ}C$ 범위인 9-10월까지 지속되었고, 성어보다는 치어에서 폐사율이 높게 나타나는 것으로 확인되었다. 폐사되는 패턴으로는 급성으로 인한 대량폐사와 소량으로 지속적으로 폐사되는 경우가 확인되었다. 능성어로부터 분리된 NNV 분리주들의 coat protein gene을 계통분석한 결과, 분리주들은 모두 RGNNV 유전자형에 속하였다. 이상의 결과로 능성어 양식장에서의 VNN은 RGNNV type의 NNV에 의해 여름철 7-9월(사육수온: 약 $24^{\circ}C$)에 치어뿐만 아니라 성어에서 발생하는 것으로 확인되었다.

• 대한수의학회지
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• 제56권2호
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• pp.125-127
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• 2016

Gradual mortality of look down fish (Selene vomer) was observed in a private aquarium in Seoul, showing abnormal swimming behavior and lethargy. A bacterial pathogen from kidney was cultured, identified, and confirmed as Vibrio harveyi using Vitek System 2 and 16S rRNA gene sequencing. A predominant bacterial strain, SNUVh-LW2 was proved to be most closely related to isolates from China by phylogenetic analysis with minimum evolution method. Also, tetracycline was considered as the most sensitive antibiotic agent via antibiotic susceptibility test. The group of fish was treated according to the diagnostic result and no more mortality was observed.

• 한국어병학회지
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• 제34권1호
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• pp.105-110
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• 2021

We experienced mortality of dark-banded rockfish (Sebastes inermis) maintained in the rearing facility located in Gangneung, after transportation. Moribund fish showed various symptoms such as exophthalmia, skin ulcers, tail rots, gill rots, discoloration of liver with petechiae, yellowish fluid in intestines and ascites. Two different colonies were dominantly appeared after spreading the lesions on the agar plates and incubation. One isolate (SI_1) showed swarming movement on TSA, and formed yellow colonies on TCBS agar. The other (SI_2) showed no swarming motility and green colonies on TCBS agar. Both of them were Gram-negative. All of these results are similar with those of Genus Vibrio. They were identified as V. harveyi and V. gigantis by PCR with subsequent sequencing of 3 different genes (16 rDNA, recA, rpoA). V. harveyi is well-known as a serious pathogen of marine fish and invertebrates, while V. gigantis is known to be often isolated from marine invertebrates, but the pathogenicity is still unknown. We suspect V. harveyi as the cause of the mortality of dark-banded rockfish, but challenge experiments with these 2 Vibrio species are thought to be necessary to make a clear conclusion.