• Title/Summary/Keyword: Fish egg development

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Artificial Spawning Behavior and Development of Eggs, Larvae and Juveniles of the Red Spotted Grouper, Epinephelus akaara in Korea

  • Park, Jong Youn;Cho, Jae Kwon;Son, Maeng Hyun;Kim, Kyong Min;Han, Kyeong Ho;Park, Jae Min
    • Development and Reproduction
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    • v.20 no.1
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    • pp.31-40
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    • 2016
  • This study was conducted in order to examine the egg development in red spotted grouper, Epinephelus akaara and the morphological development of its larvae and juveniles, and to obtain data for taxonomic research. This study was conducted in June 2013, and 50 male and female fish were used for the study. One hundred ${\mu}g/kg$ of LHRHa was injected into the body of the fish for inducing spawning, and the fish were kept in a small-sized fish holder ($2{\times}2{\times}2m$). Eggs were colorless transparent free pelagic eggs, 0.71-0.77 mm large (mean $0.74{\pm}0.02mm$, n=30), and had an oil globule. Hatching started within 27 h after fertilization. Pre-larvae that emerged just after hatching were 2.02-2.17 mm in total length (mean $2.10{\pm}0.11mm$), their mouth and anus were not opened yet, and the whole body was covered with a membrane fin. Post-larvae that emerged 15 days post hatching were 3.88-4.07 mm in total length (mean $3.98{\pm}0.13mm$), and had a ventral fin with two rays and a caudal fin with eight rays. Juveniles that were formed at 55 d post hatching, were 31.9-35.2 mm in total length (mean $33.6{\pm}2.33mm$), with red color deposited over the entire body, and black chromophores deposited in a spotted pattern. The number of fin rays, body color, and shape were the same as that in the adult fish.

Egg Development and Morphological Change of Larvae and Juveniles of the Sakhalin Sole Limanda sakhalinensis (사할린가자미(Limanda sakhalinensis)의 난발생 과정 및 자치어 형태발달)

  • Han, Kyeong-Ho;Na, Hae-Choon;Park, Ae-Jeon;Park, Jae-Min
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.3
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    • pp.287-295
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    • 2017
  • Egg development and morphological change of larvae of the Sakhalin sole Limanda sakhalinensis were studied by observing specimens obtained in a rearing experiment from fertilized eggs to the juvenile stage. The wild broodstock was collected in January 2010 and kept in a circular water tank (${\O}1.5{\times}1m$) at a temperature of $14.5{\pm}0.5^{\circ}C$. Fertilized eggs ranged from 0.72 to 0.82 mm ($0.77{\pm}0.07mm$, $mean{\pm}SD$) in diameter. The eggs were spherical, transparent and adhesive demersal. The egg yolk was divided from the oocyte 10 min after fertilization (AF), and an embryo was formed in 36 h AF. More than 50% of the eggs hatched within 133 h AF. The mouth and anus did not open until $3.5{\pm}0.25mm$ total length (TL). At 4, days after hatching (AH), the fish became larvae 3.7 to 4.2 mm ($4.0{\pm}0.36mm\;TL$), yolk absorption was completed and the mouth began to open. The left eye moved upward and the nostril moved to the right at 39 days AH. These post-larvae ranged from 8.0 to 9.9 mm TL ($8.9{\pm}1.33mm\;TL$). At 50 days AH, the fish became juveniles ($12.4{\pm}1.20mm\;TL$) There were 70-72 dorsal fin rays, 55-56 anal fin rays, 11 pectoral fin rays, and 6 ventral fin rays and the juveniles adopted a benthic life.

Effects of Different Oil Sources and Vitamin E in Breeder Diet on Egg Quality, Hatchability and Development of the Neonatal Offspring

  • An, S.Y.;Guo, Yuming;Ma, S.D.;Yuan, J.M.;Liu, G.Z.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.2
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    • pp.234-239
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    • 2010
  • Effects of polyunsaturated fatty acid (PUFA) and vitamin E (VE) supplementation in the diet of breeder hens on the egg quality and hatchability, lipid peroxides of the egg yolk, and development of the newly-hatched offspring chicks were investigated. A total of 800 Avian 48, 28 wk-old broiler breeders were assigned randomly to 4 groups with 4 replicates of 45 females and 5 males. Each group was fed one of the following four diets with different oil sources and levels of VE: corn oil (CO), fish oil (FO), CO+VE and FO+VE. The results showed that: i) Addition of FO in the breeder diet reduced the whole egg weight, yolk weight, albumen weight, yolk color score and neonatal offspring chick body weight without affecting the hatchability as compared to the CO treatment. ii) Addition of VE efficiently reduced the lipid peroxides of egg yolk from hens fed diets containing FO. iii) VE in the breeder diet significantly promoted the development of liver and heart of the chick offspring.

Early Life History of Coreoperca herzi in Han River, Korea

  • Park, Jae-Min;Jeon, Hyung-Bae;Suk, Ho Young;Cho, Seong-Jang;Han, Kyeong-Ho
    • Development and Reproduction
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    • v.24 no.1
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    • pp.63-70
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    • 2020
  • This study aimed to investigate early life of Coreoperca herzi living in Han River and compare morphological differences between different groups during the development in order to provide basic data for relevant taxonomic research. On average, one female individual spawned 541 to 861 eggs (average 701) at once. After 259 hours, the tail broke out of the egg membrane and hatching began. Immediately after hatching, the larvae were average 7.81±0.10 mm (n=5) in total length. 60 days the juvenile was average 35.9±1.30 mm (n=5) in total length. The white spots spread to the rest of the body, rending the same pattern as that on the body of their broodstock fish.

Morphological Development of Eggs, Larvae and Juveniles of the Hemibarbus longirostris (Cypriniformes : Cyprinidae) from Korea (한국산 참마자(Hemibarbus longirostris) (Cypriniformes : Cyprinidae)의 난 발생 및 자치어 형태 발달)

  • Mun, Seong Jun;Park, Jae Min;Han, Kyeong Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.2
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    • pp.203-208
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    • 2020
  • This study was carried out to clarify the larvae and juveniles of egg development and autonomous development of Hemibarbus longirostris from Korea, and to obtain basic data for species conservation and seed production. The shape of the egg was circular and sticky. The average size of the eggs was 2.01 mm (n=10). At 185 hours after fertilization, more than 50% of the total embryos were hatched. The newly hatched larvae had an average total length of 8.10 mm (n=5) and had egg yolk in the abdomen. At 3 days after hatching, the larvae absorbed all egg yolk was average total length 8.64 mm. On the 6 days after hatching, the caudal tip of the notochord started to curve upwards was average total length 10.9 mm. At 70 days after hatching, the average total length 37.9 mm. The number of fins was i 8-10 in dorsal fin, iii 7 in the anal fin, and ii 5 in the ventral fin.

Early Life History of Lefua costata (Cypriniformes : Balitoridae) from Korea

  • Park, Jae-Min;Cho, Seong-Jang;Han, Kyeong-Ho
    • Development and Reproduction
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    • v.24 no.4
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    • pp.307-316
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    • 2020
  • The purpose of this study is to observe the early life history of Korean Lefua costata and use the result as basic taxonomic research data for balitorid fish. The fertilized eggs were light green color with completely circle shape and mean size was 1.21±0.06 mm (n=30). Immediately after hatching, the size of the larvae was 2.81±0.11 mm (n=5) in mean length, with egg yolk. On the 3rd day after hatching, the preflexion larvae had a mean length of 4.64±0.09 mm (n=5), and their mouth was opened to start feeding. On the 8th day after hatching, a mean length of the postflexion larvae was 9.43±0.46 mm (n=5), the distal part of the notochord was bent to 45°, and 16 fin rays were developed on the caudal fin. On the 31st day after hatching, a mean length of juveniles was 22.3±0.85 mm (n=5), and the number of fin rays was the same as that of adult fish with (iv8) dorsal fins and (iii8) anal fins.

Spawning Behavior, Egg Development, Larvae and Juvenile Morphology of Hyphessobrycon eques (Pisces: Characidae) Characidae Fishes

  • Park, Jae-Min;Kim, Na-Ri;Han, Kyeong-Ho;Han, Ji-Hyeong;Son, Maeng-Hyun;Cho, Jae-Kwon
    • Development and Reproduction
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    • v.18 no.4
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    • pp.241-249
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    • 2014
  • Hyphessobrycon eques is a famous fish for ornamental fish market and aquarium. They are inhabit in regions of Amazon and Paraguay River basin. Serpae fishs were investigated 2-3 males are chased to female, and then males attempted to simulate the females abdomen. After fertilization, eggs were kept in incubators at $28^{\circ}C$. The fertilized eggs had adhesive and demesal characteristics and had a mean diameter of $0.92{\pm}0.01mm$. Larvae hatched at 16 hrs post fertilization. The hatched larvae averaged $2.90{\pm}0.16mm$ in total length ($L_T$). Complete yolk sac resorption and mouth opening occurred on the third day post hatching. At 45 days post hatching, the larvae were $12.5{\pm}1.60mm$ $L_T$ and had reached the juvenile stage.

Chemical Dissection of Zebrafish Egg Envelop, the Chorion

  • Hwang, C. N.;H. J. Kang;Kim, C.;D. S. Na;S. K. Chae;B. K. Joo;Lee, J. W.;Lee, S. H.
    • Proceedings of the KSAR Conference
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    • 2004.06a
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    • pp.283-283
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    • 2004
  • The eggs of most animal species are surrounded by an extracellular matrix known as chorion, egg envelope, egg coat, or zona pellucida. Development of fish embryo usually takes several days in an aquatic environment. During embryonic development, the chorion must protect embryo from physical damage and microbial infection in the exposed aquatic environment. (omitted)

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Morphological Development of Eggs, Larvae and Juvenile of the Sunrise Sculpin, Pseudoblennius cottoides (Teleostei: Cottidae) (가시망둑(Pseudoblennius cottoides)의 난발생 및 자치어 형태발달)

  • YOO Dong-Jae;HAN Kyeong-Ho;BAEK Seung-Rok;KIM Kwang-Su;HA Sung-Chan;ZANG Hu-Chun;LEE Gi-Seok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.3
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    • pp.263-269
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    • 2003
  • Morphological development of eggs, larvae and juvenile of the sunrise sculpin, Pseudoblennius cottoides were studed in the field and laboratory at the coastal Dolsan-do, Yeosu-shi from October, 2000 to April, 2001 Egg mass of Pseudoblennius cottoides in peribranchial cavity of Halocynthia hilgendorfi, were observed during late fall to winter in the study area. Fertiliged eggs were spherical in shape, demersal, adhesive, transparent and greenwish yellow color, measuring 1.84 mm (1.83-1.87 mm) in diameter. There were numerous and 17 (15-20) various-sized oil globules accounted in the yolk. Granular materials formed a mass in the yolk. Fertiliged eggs hatched at 301 hr 20 min after morula stage. Newly hatched larvae 6.31 mm (6.24-6.37 mm) in total length (TL), had a large yolk. At 3 days after hatching, the larvae, 6.77 mm (6.69-7.14 mm) in TL came out through the excurrent siphon of Halocynthia hilgendorfi. At 13 days after hatching, the larvae 12.59 mm (12.42-12.63 mm) in TL transformed to postlarval stage. At 32 days after hatching, postlarvae of TL 19.18 mm (19.01-19.46 mm) have reached the juvenile stage.

Ovarian Development and Reproductive Cycle of the Female Red Tongue Sole, Cynoglossus joyneri (Teleostei: Cynoglossidae) (참서대 (Cynoglossus joyneri) 암컷의 난소발달 및 생식주기)

  • LEE Jung Sick;KIM Sung Yeon;MA Kyung Hwa;HUH Sung Hoi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.6
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    • pp.554-558
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    • 2000
  • Reproductive biology of the female red tongue sole, Cynoglossus joyneri was investigated by means of histological methods. Gonadosomatic index (GSI) was the highest in June. Developmental pattern of oocytes was group-synchronous. Egg stalk and yolk nucleus were observed in the early growing oocytes. Reproductive cycle could be classified into the growing ($February{\~}May$), maturation ($May{\~}June$), ripe and spent ($June{\~}August$), and recovery and resting ($August{\~}February$). The absolute fecundity per individual ($TL 28.1{\~}30.8 cm$) was 2,197 and relative fecundity per g body weight was 18.0.

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