• 한국수산과학회지
• /
• 제56권4호
• /
• pp.532-540
• /
• 2023

This study aimed to evaluate the effects of dietary supplementation with two different types of hydrolyzed blood meal (HBM) on the growth performance, feed utilization, digestibility and innate immunity of olive flounder Paralichthys olivaceus. A control diet (Con) consisting of 60% fish meal was formulated and four diets containing two different types of HBM at varying concentrations were prepared 2.5 and 5.0% liquid HBM (L2.5 and L5.0) and 0.5 and 1.0% powdered HBM (P0.5 and P1.0). A total of 450 olive flounder (average body weight: 50±0.07 g) were distributed in 15 tanks (240 L), with three replicate groups per diet. The fish were fed the diets to apparent satiation for 9 weeks and subsequently exposed to Edwardsiella tarda. The results showed that fish fed L2.5, L5.0 and P0.5 diets exhibited significantly higher lysozyme activity compared to those fed the Con and P1.0 diets. During the challenge test against E. tarda, the L5.0 and P0.5 fish groups exhibited higher disease resistance than that of the Con group. These findings indicate that dietary supplementation with HBM could positively effect the innate immunity and disease resistance of olive flounder.

• 한국어병학회지
• /
• 제21권3호
• /
• pp.259-270
• /
• 2008

Disease surveillance was performed to monitor the prevalence of fish pathogens in wild marine fish caught in coastal offshore water in Korea. A total of 333 of fish samples were collected at set net or fish market at landing port in Pohang (East Sea), Taean (Western Sea), Goseong and Tongyeong (Southern Sea) and 21 species of pathogens causing clinical infections to farmed fish were investigated. The detection rates of fish pathogens from Mugili formes, Tetraodontiformes, Pleuroneciformes, Sorpaeniformes, erciformes and Clupeiformes were 90.9, 61.1, 47.6, 43.6, 37.2 and 11.8%, respectively. Comparing with prevalence of diseases seasonally, both the detection rates of bacteria and parasite were higher than those of virus in April but the detection rates of parasites were distinctively higher than those of bacteria in August with high water temperature. Virus were detected in fish samples caught in the Western and Southern Sea in April. The detected parasites were Trichodina, Ichthyophthirius, Dactylogyrus, Microcotyle, Bivagina, Caligus, Alella and Myxobolus. Among the bacterial pathogens, Vibrio, Streptococcus, Photobacterium, Psuedomonas were predominant. Viral nervous necrosis virus (VNNV) and flounder lymphocystis disease virus (FLDV) were detected from the 6 species of fish virus examined in this study.

• 한국양식학회:학술대회논문집
• /
• 한국양식학회 2001년도 추계 한국양식학회 학술대회
• /
• pp.227.2-228
• /
• 2001

• 한국어병학회지
• /
• 제12권2호
• /
• pp.107-113
• /
• 1999

능성어의 바이러스성 신경괴사증 바이러스(fish nodavirus)의 병원성을 능성어 및 다른 해산어류를 대상으로 인위감염법에 의해 조사한 결과, 능성어 바이러스성 신경괴사증 바이러스는 능성어에서는 소형어(27 g)부터 대형어(104 g)까지 제사를 일으켜 강한 병원성을 나타냈지만, 실험에 사용한 크기의 방어(537 g), 참돔(207 g), 돌돔(43 g), 넙치(41 g), 자주복(27 g) 및 조피볼락(94 g)에는 폐사를 일으키지 않아 병원성을 나타내지 않았다. 그리고 사육수온별 능성어에 대한 바이러스의 병원성은 수온 $15^{\circ}C$에서는 병원성을 나타내지 않았고, 수온 $20\sim28^{\circ}C$에서는 수온이 높을 수록 병원성을 강하게 나타냈다. 그리고 바이러스는 근육주사, 복강 주사 및 경구감염뿐만 아니라 침지감염과 혼합사육감염에 의해서도 쉽게 능성어에 감염이 되지만, 감염 후 생존한 능성어에 대해서는 바이러스를 재감염시켜도 폐사가 일어나지 않았다.

• 한국어병학회지
• /
• 제34권1호
• /
• pp.9-15
• /
• 2021

Rock bream (Oplegnathus fasciatus) is a highly valued aquaculture species in Korea. However, the aquaculture industry suffers huge economic losses due to rock bream iridovirus (RBIV) infection in summer. The objective of this study was to determine genetic diversity and relationships of DNAs isolated from two groups of rock bream after RBIV infection using five microsatellite (MS) markers. The first group of fish died early and the second group of fish died later after RBIV infection. In this experiment, 90 fish (5.1±1.0 cm and 4.1±1.3 g) were injected with 50 μl of RBIV (10⁴ TCID⁵⁰/ml) and maintained at 26℃ for 15 days. Genomic DNAs were extracted from fins of 20 fish that died earlier or later after RBIV infection. These DNAs were subjected to genotyping using five MS markers (CA-03, CA3-05, CA3-06, CA-10, and CA3-36). Of these markers, CA3-05 (early death group), CA3-06 (late death group), and CA3-36 (both early and late death groups) showed different alleles distribution rates. In-depth studies are needed to provide valuable information for selecting RBIV-resistant fish. In conclusion, microsatellite marker distribution pattern differences between early- and late- death groups of rock bream after RBIV infection showing different RBIV susceptibilities were determined using MS markers and genotyping. Results of this study suggest that MS markers could be used to facilitate the selection of RBIV resistant rock bream.

• Journal of Animal Science and Technology
• /
• 제52권4호
• /
• pp.337-346
• /
• 2010

우리 연구팀은 넙치를 대상으로 사료 내 발효마늘분말(fermented garlic powder, FGP)의 첨가에 의한 비특이적 면역반응과 어병세균(V. anguillarum, S. iniae, E. tarda)에 대한 질병 저항성에 대해 처음으로 보고한다. 동일한 단백질 함량(45%)과 칼로리(17.1 MJ/kg)를 갖은 4개의 실험사료는 발효마늘분말이 0%, 0.5%, 1% 및 2% (G-0, G-0.5, G-1 및 G-2)가 포함되도록 조성되었다. 실험은 3반복구로 수조 당 30마리의 어류를 대상으로 유수식 시스템에서 수행되었다. 5주간의 성장실험 종료 후, 각 수조에서 건강한 어류를 선별하여 어류의 항병력을 평가하기 위해 3가지 박테리아 부유액(1 ml)을 복강에 주사하였다. 5주간의 성장실험기간 동안에 성장률, 일간성장률, 사료전환효율, 단백질전환효율 및 생존율에서는 실험사료에 의한 유의적인 차이가 없었다. 그러나 사료섭취율은 발효마늘분말이 2% 첨가된 그룹에서 대조구와 비교하여 유의적으로 낮았다. 헤모글로빈, 헤마토크릿, 대식세포 활성 및 라이소자임 활성에서는 사료 내 발효마늘분말 함량이 증가함에 따라 유의적으로 증가하였다. 발효마늘분말 0.5% 첨가구의 혈장 중성지질은 대조구보다 유의적으로 낮았다. 누적사망률은 E. tarda의 경우를 제외한 모든 공격실험에서 발효마늘분말이 포함된 사료를 섭취한 어류가 대조구와 비교하여 유의적으로 낮았다. 이번 연구의 결과를 볼 때, 사료 내 발효마늘분말의 첨가는 넙치에 있어서 V. anguillarum과 S. iniae에 대한 항병력 및 비특이적 면역반응을 향상시킨 것으로 사료된다.

• 한국어병학회지
• /
• 제33권1호
• /
• pp.55-62
• /
• 2020

본 연구에서는 점액포자충에 의한 넙치의 여윔증 치료 후보물질을 탐색하기 위하여, Coxi-stop, Coxiclin 및 BLEAN80 등의 제품을 사용하여 in vitro 및 in vivo 실험을 실시하였다. 톨트라주릴이 주성분인 Coxi-stop의 경우, BF-2 cell을 사용한 in vitro 실험에서 점액포자충의 활성을 감소시키는 결과를 보였고, cell lysis와 같은 세포 독성 현상이 나타나지 않았다. In vivo 실험에서 Coxi-stop을 사용하여 약욕한 실험군은 대조군에 비하여 누적폐사율이 낮게 나타났으며, 이것은 톨트라주릴이 어류 기생충성 질병에 치료 효과가 있다는 기존의 보고와 유사한 결과이다. 본 연구에서는 톨트라주릴이 넙치의 여윔증 치료제로서 가능성이 있는 후보 물질이라는 것을 제시한다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권4호
• /
• pp.1235-1240
• /
• 2012

Background: Opisthorchis viverrini infection is a serious public-health problem in Southeast Asia. It is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the etiology of cholangiocarcinoma. Objectives: This study aimed to investigate Opisthorchis viverrini metacercarial infection in cyprinoid fish collected from 32 districts of Nakhon Ratchasima province, Northeastern Thailand during one year period from February 2010 to February 2011. Methods: A cross-sectional study was conducted, data being collected with pepsin-HCl digestion and stereomicroscope, respectively. Analysis was performed using SPSS Windows Version 12.0. Results: A total of 640 Cyprinidae family fish including 5 species were collected from different study sites, and investigated for O. viverrini metacercariae. The infection rate was 12.3% (79/640), predominantly in Cyclocheilichthys armatus, C. repasson, Puntioplites proctzysron, Hampala macrolepitota and Hampala dispar, respectively. The prevalence of O. viverrini metaceria in Nakhon Ratchasima area was 78.1%, predominantly in Sida and KiaKham Thale So. Conclusion: This findings stress that natural fish species in rural communities are still a source of O viverrini infection and put local people at risk, therefore public awareness and prevention campaigns are urgently required.

• 한국어병학회지
• /
• 제35권2호
• /
• pp.215-223
• /
• 2022

Disease including parasite, bacteria and virus cause serious mortality to salmonid fish in the aquaculture. In this study, we investigated the current disease status of the rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch) in Yanayang, Pyeongchang, Jeongseon and Yeongwol of Gangwon province in 2021 and performed molecular characterization of those pathogen. For parasites, Ichthyophthirius multifiliis was observed at 2 farms. For bacteria, we identified Aeromonas sobria from kidney of rainbow trout using phylogenetic analysis of gyrB gene. A. salmonicida were isolated from necrosis site of gill cover and fin in coho salmon and necrotic lesion of fin in rainbow trout. Phylogenetic analysis using vap gene indicated that A. salmonicida isolated in this study were clustered with previously reported A. salmonicida subsp. salmonicida isolates. For virus, JRt-Nagano type of infectious haematopoietic necrosis virus was detected in rainbow trout, but infectious pancreatic necrosis virus and Oncorhynchus masou virus were not detected. These results provide useful information for the prevention of disease spread and transmission when cultivating new species such as Atlantic salmon in Korea.

• 한국어병학회지
• /
• 제28권3호
• /
• pp.133-143
• /
• 2015

The antigenic proteins of Lymphocystis disease virus (LCDV) from tumors of olive flounder, Paralichthys olivaceus, are described following characterization by mass spectrometry. In SDS-PAGE, predominant protein bands were observed at 114, 88, 70, 54, 52, 47, 42 and 24 kDa. Western blot analysis showed that antisera reacted strongly at molecular weights of 114, 67 and 54 kDa, and reacted weakly at molecular weights of 74, 70, 36, 24 and 22 kDa. In the identification of LCDV antigenic proteins by matrix-assisted laser desorption ionization (MALDI) TOF mass spectrometry, 10 of 14 excised bands consisted mostly of proteins with amino acid sequences that matched LCDV-C (lymphocystis disease virus isolate China) ORFs. Strong antigens with molecular weights of 114, 67 and 54 kDa were identified as LDVICp236 (chromosome segregation ATPase), LDVICp033 (membrane bound metallopeptidase) and LDVICp157 (hypothetical protein), respectively. Minor antigens with molecular weights of 70, 36, 24 and 22 kDa proteins were identified as LDVICp160 (acetyl-coA hydrolase), LDVICp213 (hypothetical protein), LDVICp039 (hypothetical protein) and LDVICp213 (hypothetical protein). However, the major capsid protein (LDVICp043) did not react with the polyclonal antibody.