Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Microsatellite marker distribution pattern in rock bream iridovirus (RBIV) infected rock bream, Oplegnathus fasciatus

  • Jung, Myung-Hwa (Department of Marine Bio and Medical Sciences, Hanseo University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, Chonnam National University)
  • Received : 2021.06.09
  • Accepted : 2021.06.15
  • Published : 2021.06.30
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Abstract

Rock bream (Oplegnathus fasciatus) is a highly valued aquaculture species in Korea. However, the aquaculture industry suffers huge economic losses due to rock bream iridovirus (RBIV) infection in summer. The objective of this study was to determine genetic diversity and relationships of DNAs isolated from two groups of rock bream after RBIV infection using five microsatellite (MS) markers. The first group of fish died early and the second group of fish died later after RBIV infection. In this experiment, 90 fish (5.1±1.0 cm and 4.1±1.3 g) were injected with 50 μl of RBIV (104 TCID50/ml) and maintained at 26℃ for 15 days. Genomic DNAs were extracted from fins of 20 fish that died earlier or later after RBIV infection. These DNAs were subjected to genotyping using five MS markers (CA-03, CA3-05, CA3-06, CA-10, and CA3-36). Of these markers, CA3-05 (early death group), CA3-06 (late death group), and CA3-36 (both early and late death groups) showed different alleles distribution rates. In-depth studies are needed to provide valuable information for selecting RBIV-resistant fish. In conclusion, microsatellite marker distribution pattern differences between early- and late- death groups of rock bream after RBIV infection showing different RBIV susceptibilities were determined using MS markers and genotyping. Results of this study suggest that MS markers could be used to facilitate the selection of RBIV resistant rock bream.

Keywords

Acknowledgement

This research was a part of a project entitled 'Fish Vaccine Research Center' funded by the Ministry of Oceans and Fisheries, Republic of Korea.

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