• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1060-1066
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• 2005

Under environmental stress, such as strong irradiance or nitrogen deficiency, unicellular green algae of the genus Haematococcus accumulate secondary carotenoids, i.e. astaxanthin, in the cytosol. The induction and regulation of astaxanthin biosynthesis in microalgae has recently received considerable attention owing to the increasing use of secondary carotenoids as a source of pigmentation for fish aquacultures, and as a potential drug in cancer prevention as a free-radical quencher. Accordingly, this study generated expressed sequence tags (ESTs) from a library constructed from astaxanthin-induced Haematococcus pluvialis. Partial sequences were obtained from the 5' ends of 1,858 individual cDNAs, and then grouped into 1,025 non-overlapping sequences, among which 708 sequences were singletons, while the remainder fell into 317 clusters. Approximately $63\%$ of the EST sequences showed similarity to previously described sequences in public databases. H. pluvialis was found to consist of a relatively high percentage of genes involved in genetic information processing ($15\%$) and metabolism ($11\%$), whereas a relatively low percentage of sequences was involved in the signal transduction ($3\%$), structure ($2\%$), and environmental information process ($3\%$). In addition, a relatively large fraction of H. pluvialis sequences was classified as genes involved in photosynthesis ($9\%$) and cellular process ($9\%$). Based on this EST analysis, the full-length cDNA sequence for superoxide dismutase (SOD) of H. pluvialis was cloned, and the expression of this gene was investigated. The abundance of SOD changed substantially in response to different culture conditions, indicating the possible regulation of this gene in H. pluvialis.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1195-1206
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• 2020

Beringraja pulchra, Cham-hong-eo in Korean, is a mottled skate which is belonging to the cartilaginous fish. Although this species is economically valuable in South Korea as an alkaline-fermented food, there are few microbial studies on such fermentation. Here, we analyzed microbial changes and pH before, during, and after fermentation and examined the effect of inoculation by a skin microbiota mixture on the skate fermentation (control vs. treatment). To analyze microbial community, the V4 regions of bacterial 16S rRNA genes from the skates were amplified, sequenced and analyzed. During the skate fermentation, pH and total number of marine bacteria increased in both groups, while microbial diversity decreased after fermentation. Pseudomonas, which was predominant in the initial skate, declined by fermentation (Day 0: 11.39 ± 5.52%; Day 20: 0.61 ± 0.9%), while the abundance of Pseudoalteromonas increased dramatically (Day 0: 1.42 ± 0.41%; Day 20: 64.92 ± 24.15%). From our co-occurrence analysis, the Pseudoalteromonas was positively correlated with Aerococcaceae (r = 0.638) and Moraxella (r = 0.474), which also increased with fermentation, and negatively correlated with Pseudomonas (r = -0.847) during fermentation. There are no critically significant differences between control and treatment. These results revealed that the alkaline fermentation of skates dramatically changed the microbiota, but the initial inoculation by a skin microbiota mixture didn't show critical changes in the final microbial community. Our results extended understanding of microbial interactions and provided the new insights of microbial changes during alkaline fermentation.

• 한국수산과학회지
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• 제40권2호
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• pp.84-94
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• 2007

The comparative feeding ecology of greenling Hexagrammos otakii and Schlegel's black rockfish Sebastes schlereli populations was investigated in the Jangbong tidal flat near Incheon, Korea. Monthly samples were taken using a modified otter trawl from November 1999 to January 2001. The stomach contents of 124 greenling and 115 Schlegel's black rockfish were analyzed. The diet of H. otakii was dominated by the amphipods Gammaropsis iaponicus, Caprella scaura, Isaeopsis sp. and Monocorophium acherusicum, the shrimps Latreutes mucronatus and Alpheus japonicus, the mysid Neomysis orientalis and the bivalve Mytilus edulis. In contrast, S. schlegeli mainly fed on the mysids N. orientalis, N japonica, and N. awatschensis, the shrimps Exopalaemon carinicauda, A. japonicus and L. mucronatus, and the fish Pholis fangi and unidentified gobiids. A comparison of the frequency and abundance of food items showed that crustaceans (e.g. amphipods, mysids and shrimps) were important foods for both species. Their main dietary components, however differed which is probably due to differences in the spatial distribution of the two species and their prey items. In conclusion, greenling and Schlegel's black rockfish, despite being sympatric on a tidal flat have distinct food habits and low dietary overlap (Schooner's index: 0.1). These results indicate, to some extent, resource partitioning for the maximum utilization of available foods in the tidal flat.

• 한국수산과학회지
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• 제27권2호
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• pp.183-192
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• 1994

대서양 남빙양 새우의 주된 분포역은 South George Island, Laurie/Coronation Islands와 Livingston/King George Islands 지역으로 공간적인 구분을 뚜렷이 하였다. 어획 수심은 층에서 150 m층까지 달했고, 10 m층으로 구분한 단위노력당어획량의 수직 분포는 층간에 큰 차이가 없었으나 수심이 깊어 질수록 낮아지는 경향을 보였다. 수온과 단위노력당 어획량의 관계로 부터 남빙양 새우는 주로 $0.8{\sim}l.0^{\circ}C$ 범위에서 높은 밀도를 나타내었다. 남빙양 새우의 암수별 체장 조성은 큰 차이를 보여 숫컷의 비율은 작은 체장에서, 암컷의 비율은 큰 체장에서 각각 높았다. 두흉갑장과 체장관계, 체장과 체중관계가 암수별로 추정되었다. 암수별 성비조성은 숫컷이 $60.3\%$, 암컷이 $39.7\%$로서 유의한 차이를 나타내었다.

• 한국수산과학회지
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• 제44권1호
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• pp.78-84
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• 2011

We investigated the stomach contents of finless porpoises collected in the Yellow Sea. Prey organisms in the stomachs of 109 finless porpoises were identified as 11 species of fish, 8 species of shrimp and 4 species of cephalopod. Index of Relative Importance analysis revealed that the porpoises fed mainly on the Japanese sand shrimp (Crangon affinis). The energy density of the prey was estimated to be 5.46 kJ. The daily energy requirement was estimated to be 18,051 kJ/day for a porpoise with an average weight of 32.49 kg. The estimated annual consumption by finless porpoises in the Yellow Sea was 25,454 tons. The average commercial catch in the Yellow Sea by Korean fisheries was 135,913 tons from 2005 to 2009. Assuming that the abundance of finless porpoises is stable, porpoises ate approximately 18.7% of the commercial catch. The fishery catch may be influenced by the consumption by finless porpoises, while fishery bycatch is a cause of porpoise mortality in the Yellow Sea.

• Ocean and Polar Research
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• 제34권2호
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• pp.165-173
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• 2012

A 26 day experiment was conducted to determine the effects of feeding and starvation on the survival, morphology, and histology in chum salmon (Oncorhynchus keta) fry. We included three experimental groups: starved, fed, and initial. The survival and growth rates were lower in the starved group than in the fed group (P < 0.05). In the starved group, survival began to decline after 16 days, and all fish had died after 26 days. We determined the effects of starvation on the morphometric parameters using the truss and classical dimensions. The dimensions in the head region were larger in the starved group than in the initial and fed groups. In contrast, the truss dimensions of the fed group were larger than those of the initial and starved groups. Starvation reduced the heights of the hepatocyte nuclei and of the intestinal epithelium (P < 0.05). The starved group also showed atrophy of the digestive structures and shrinkage of the foregut and midgut. Starvation led to the degeneration and atrophy of the exocrine pancreas, in which the lumen was markedly diminished and the folds of mucosa were less apparent. The hepatocyte morphology in the starved group was abnormal compared with that of the initial and fed groups, with highly compact, irregularly shrunken nuclei. Melanomacrophages were randomly distributed in the kidneys of the starved group, and their abundance increased rapidly during the experiment. In contrast, neither the initial nor fed group had any melanomacrophages. These results suggest that the nutritional parameters used in this study are useful indices of nutritional status in chum salmon.

• 한국해양학회지:바다
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• 제6권3호
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• pp.164-179
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• 2001

1991년부터 1994년 사이에 한국 근해에서 대형선망에 어획된 수산생물의 종조성, 어획량, 분포의 계절 및 연 변동 양상을 파악하였다. 조사 기간 중 전체의 0.1%이상을 차지하는 주요 종은 19종이 출현하였으며, 그중 고등어(Scomber japonicus)가 전체 생체량의 54.7%, 정어리 (Sardinops melanostictus) 20.4%, 전갱이 (Trachurus japonicus) 9.1% 그리고 삼치(Scomberomorus niphonius) 6.2%로 이들 부어류 4종이 전체의 90% 이상을 차지하였다. 어획노력량은 봄부터 높아져 여름에 최고였고 가을철까지 높았으나, 단위노력당 어획량은 가을부터 높아져 겨울에 최고를 보였고 봄까지 높게 나타났다. 이는 산란과 섭이를 위해 남북 이동을 하여 여름동안 분산되었던 부어류가 겨울철에는 월동장으로 회유하여 위집됨으로써 어획률이 높았기 때문으로 판단된다.

• 수산해양기술연구
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• 제43권4호
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• pp.241-250
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• 2007

Fishes were caught by a bottom trawl in the Yeo-ja bay of Korea by every three month(March, June, September and December) and seasonal variation of abundance and species composition were studied in 2005. The catches were 1,187kg and were composed of 115 species with 20 hauls by 5 stations. The dominant species caught were Charybdis bimcaulata, Crangon hakodatei, Parapenaeus fissurus, Oratosguilla oratoria, and Thryssa purava. One hundred fifty species were identified as fish(38%), crustacea(20%), mollusca(4%) and others(38%) which including of Asterias amurensis. The amount of species was high in June and September and low in March and December. The diversity index(H') was about 1.2－2.5 and station similarity of fishes caught among the community was 0.009－0.230.

• 한국수산과학회지
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• 제6권1_2호
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• pp.49-57
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• 1973

우리나라 동안의 꽁치에 대해서 1968년, 1970년 및 1971년 $1\~7$월의 어획통계 자료와 1968년의 체장측정자료를 이용하여 꽁치의 체형군에 따른 회유를 고찰한 결과는 다음과 같다. 1. 어장내의 전어군의 분포중심의 이동은 북상 회유에서나 남하 회유에서나 대체로 $130^{\circ}\~130^{\circ}30'$의 범위를 중심으로 이루어지고 있으며, 각월의 동서 방향으로의 편배의 정도는 해에 따라 대단히 심하다. 이 중심의 이동은 해황외에 해에 따른 체형군별의 자원량의 크기와도 깊은 관계를 가질 것이라 추측된다. 2. 체형군을 분류하는 기준체장의 주년에 걸친 변화곡선을 구한 결과는 다음과 같다. 5, 6월에 있어서 중형군을 구분하는 체장의 변화곡선은 $Y=24.807X^{0.0885}$이며, 중형군과 소형군을 구분하는 체장의 변화곡선은 $Y=21.532X^{0.1177}$이다. 단 Y는 체장, X는 역상의 월을 표시함. 3. 회유에 있어서 북상기에는 체형이 작은 것이 먼저 북상하고 남하기에는 체형이 큰 것이 먼저 남하하는 경향을 보인다. 4. 회유경로는 어장내의 분포중심의 이동방향으로서 추적될수 있는 것이 아니며, 1968년의 각 체형군의 회유경로는 Fig. 6과 같이 추정된다.

• 한국수산과학회지
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• 제24권4호
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• pp.248-254
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• 1991

1990년 8월 아산만에서 otter trawl을 이용하여 저어류를 채집하여 정점을 대표할 수 있는 저어류 군집 자료수집 방법을 추정하였다. 조사기간 중 총 17종, 957개체, 21,840g의 어류가 채집되었으며, 참서대 (Cynoglossus joyneri), 곤어리 (Thrissa koreana), 쥐노래미 (Hexagrammos otakii)와 민태 (Johnius belengerii)가 출현 빈도도 높고 개체수와 생체량에서 우점하였다. 주야 10회 반복 채집결과 종조성은 주야에 따라 유의한 차이를 보이지 않았으나, otter trawl 20분 예인의 개체수 자료는 우점종의 경우는 평균에 대하여 $2.2-385.1\%$, 전어종은 $52.2-162.0\%$의 넓은 변이를 보였다 채집면적을 늘려 갔을 때 누적종수는 예인 4회 자료 누적 이후에는 증가가 둔화되었고 종다양성지수는 3회 이후 거의 점관선에 도달하였다. 개체수의 평균에 대한 분산은 자료를 누적시켰을 때 감소하여 4회 이후에는 자료가 Poisson 분포 양상을 보였다. Otter trawl을 이용하여 아산만 저어류를 채집할 때 20분 예인 4회 이상의 자료를 합하면 정성, 정량적으로 한 조사정점을 대표할 수 있는 것으로 판단된다.