• The Korean Journal of Malacology
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• v.21 no.2 s.34
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• pp.81-93
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• 2005

Reproductive cycle with the gonadal phases, first sexual maturity, artificial spawning amount by the size and spawning interval of the hard clam, Meretrix lusoria were investigated by histological observations and morphometric data by artificial spawning induction. Meretrix lusoria is dioecious and oviparous. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (February to May), ripe stage (April to August), partially spawned stage (June to September), and spent/inactive stage (September to February). The spawning period was from June to September, and the main spawning occurred between July and August when the seawater temperature exceeds over $20^{\circ}C$. Percentage of first sexual maturity of female and male clams ranging from 40.0 to 45.0 mm in shell length was over 50%, and all clams over 50.0 mm in shell length sexually matured. Female and male clams ranging from 40.0 to 45.0 mm in shell length are considered to be two years old. Therefore, we assume that the hard clams of both sexes begin reproduction from two years of age. The mean number of the spawned eggs increased with the increase of size (shell length) classes. In case of artificial spawning induction, the number of spawned eggs from the clams of a sized class was gradually decreased with the increase of the number of the spawning frequencies (the first, second, and third spawnings). In the experiments of artificial spawning induction during the spawning season, the interval of each spawning was estimated to be 15-18 days (average 17 days).

• The Korean Journal of Malacology
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• v.21 no.1
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• pp.1-11
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• 2005

Reproductive cycle, first sexual maturity, spawning amount related with the size and spawning interval in female Ruditapes philippinarum were investigated by histological observation and the analysis of morphometric data during artificial spawning induction. Ruditapes philippinarum is dioecious and oviparous. The reproductive cycle of this species can be subdivided into five successive stages: early active stage (January to March), late active stage (February to May), ripe stage (April to August), partially spawned stage (May to October), and spent/inactive stage (August to February). The spawning period was once a year between May and early October, and the main spawning occurred between July and August when seawater temperature was approximately $20^{\circ}C$. Percentages of first sexual maturity of female clam of 15.1-20.0 mm in shell length were 56.3%, and 100% for the clams > 25.1 mm. The mean number of the spawned eggs increased with the increase of size classes (shell length). In case of spawning induction by the same size class, the number of spawned eggs were gradually decreased with the increase of spawning frequencies (the first, second, and third spawnings). In the experiments of artificial spawning induction during the spawning season, the interval of each spawning was estimated to be 15-17 days (average 16.5 days).

• Development and Reproduction
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• v.16 no.3
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• pp.205-217
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• 2012

The ovarian cycle, the biological minimum size, and artificial spawning frequency by artificial spawning induction of the female hard clam, Meretrix petechialis, were investigated by histological observations and morphometric data. The ovarian cycle of this species can be classified into five successive stages: early active stage, late active stage, ripe stage, partially spawned stage, and spent/inactive stage. The spawning period was from June to September, and the main spawning occurred between July and August when the seawater temperature exceeds over $20^{\circ}C$. The biological minimum size (shell length at 50% of first sexual maturity) in females were 40.39 mm in shell length (considered to be two years of age), and all clams over 50.1 mm in shell length sexually matured. In this study, the mean number of the spawned eggs by spawning induction increased with the increase of size (shell length) classes. In case of artificial spawning induction for the clams > 40.39 mm, the number of spawned eggs from the clams of a sized class was gradually decreased with the increase of the number of the spawning frequencies (the first, second, and third spawning). In the experiments of artificial spawning induction during the spawning season, the interval of each spawning of this species was estimated to be 15-18 days (approximately 17 days).

• Fisheries and Aquatic Sciences
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• v.4 no.4
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• pp.208-218
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• 2001

We have investigated the gonad index (GI), gonadal development, reproductive cycle, first sexual maturity, sex ratio, the number of spawned eggs and spawning frequency of the Manila clam, Ruditapes philippinarum. Samples were collected from the intertidal zone of Komso Bay, Korea from January to December in 1999. Monthly changes in the gonad index (GI) and condition index showed a similar pattern in the reproductive cycle. The spawning period was once a year between early June and early October, there was a spawning peak between July and August when seawater temperature was over $20^{\circ}C$. The reproductive cycle of this species can be categorized into five successive stages; early active (February to March), late active (April to May), ripe (April to August), partially spawned (June to October), and spent/inactive stage (August to March). Percentages of first sexual maturity of female and male clams of l5.1-20.0mm in shell length were $56.3\%$ and $60.0\%$, respectively, and $100\%$ for the clams >25. mm. The sex ratio of individuals >15.1 mm in shell length was about 1:1 $(\chi^2= 0.02,\;p>0.05)$. Number of the eggs released from each clam by the induction increased as the size of clam in terms of shell length increased. Mean number of the eggs from the second induction of the spawning was $75.35-84.30\%$ $(average\;79.81\%)$ of the number of the eggs released in the first spawning. Our data indicated that R. philippinarum in Komso Bay has one major spawning peak with over two minor spawning, and the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.

• Journal of Aquaculture
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• v.4 no.2
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• pp.85-95
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• 1991

Spawning inducement of flounder, Paralichthys olivaceus, was attemped by the control of water temperature and photoperiod from June 1, 1989 through January 5, 1990 (189 days) Water temperature was gradually decreased from $21.7^{\circ}C$ to $10.6^{\circ}C$ which was the minim biological temperature for spawning of flounder. Water temperature was increased asain to $15^{\circ}C$ gradually, and then maintained at this level through the end of spawning. Photoperiod was also changed gradually from 10L/14D in June 1, 1989 to 14L/10D in July 25, 1989. Spawning of the fish occurred from October 4, 1989 through January 5, 1990 (93 days). The average number of eggs spawned during the spawning season per female were 2.67 billion. The first spawning occurred on the 60th day after the time of minimun water temperature $10.6^{\circ}C$. The water temperature and photoperiod at the first spawning were $13^{\circ}C$ and 14L/10D respectively It took 71 days to spawn since the photoperiod had changed from 10L/14D to 14L/10D. Spawning period can be devided into three terms. The first term was continued for 30 days from the beginning of the spawning. The second term for 41 days was the major spawning period followed by the third term, the final period of the spawning, for 22 days. The percentage of average fertilization rate of the eggs in the first, second and third spawning terms were $37.4{\%},\;54.1\%\;and\;19.6\%$, respectively. Feeding rate was increased in the maturing period but, decreased in the spawing period. During the final spawning period, the abrubt decreasing of the feeding occurred again.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.153-153
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• 2003

Reproductive cycle with the ovarian developmental phases, first sexual maturity of the female venus clam, Cyclina sinensis, was investigated by a histological study, and the number of spawned eggs and spawning frequency by artificial spawning induction of the clams were estimated. Samples were collected from the intertidal zone of Simpo, Gimje city, Korea between January and December, 2001. The reproductive cycle of this species can be classified into five successive stages; early active (February to April), late active (March to June), ripe (May to August), spent (July to September), and spent and inactive (September to February). The spawning period was once a year between July and August, a spawning peak was seen in July and August. Percentages of first sexual maturity of female clams of 25.1-30.0 mm in shell length were 64.3%, and 100% for the clams > 40.1 mm. The number of the eggs released from each clam by spawning induction increased as the size of clam in terms of shell length increased. The mean number of the eggs released from the second induction of spawning was 76.87% of the number of the eggs released in the first spawning. Our data indicated that the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.

• Korean Journal of Ichthyology
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• v.26 no.1
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• pp.11-16
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• 2014

The best spawning timing in a day and the first spawning position of Pseudopungtungia nigra was investigated at the Jujacheon stream in Jinan-gun Jeollabuk-do, Koera from May to July 2009. P. nigra spawned only at the nest of Coreoperca herzi from 7 May to 11 July, 2009. P. nigra spawned at 56 nests among 61 nests of C. herzi, and the spawning of P. nigra started on after 1st or 2nd day spawning of C. herzi. The spawning behavior was dominantly observed at around 06:00 to 07:00 AM, when parental C. herzi males are usually inactive in the territorial defence. The hatching rate of P. nigra eggs was closely related with the parental activity of the guarding C. herzi, and P. nigra deposit their eggs as close as possible to the egg clutches of C. herzi (normally at the perimeter of the clutch), likely to take much of the potential effects from fanning and guarding, as the hosts focus their care on the centre of the egg clutch.

• The Korean Journal of Malacology
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• v.28 no.4
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• pp.363-375
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• 2012

The gametogenic cycle, the number of spawning seasons per year and first sexual maturiity of the pen shell, Atrina (Servatrina) pectinata, were investigated by quantitative statistical analysis using an Image Analyzer System. Compared two previous results (the spawning periods in the reproductive cycles in 1998 and 2006) by qualitative histological analysis with the present results by quantitative statistical analysis, there are some differences in the spawning periods: the spawning period (June to September) by quantitative statistical analysis was one month longer than those of two previous reports (June to July or June to August) by qualitative histological analysis. However, the number of spawning seasons studied by the qualitative and quatitative analyses occurred once per year. In quantitative statistical analysis using an image analyzer system, the patterns of monthly changes in the percent (%) of the areas occupied by follicles to the ovary area in females (or that of the areas occupied by spermatogenic stages to the testis area in males) showed a maximum in May, and then sharply droped from June to September, 2006. From these data, it is apparent that the spawning season of A. (S.) pectinata occurred once a year from June to September, indicating a unimodal gametogenic cycle during the year. Shell heights of sexually mature pen shells (size at 50% of group sexual maturity, $GM_{50}$) that were fitted to an exponential equation were 15.81 cm in females and 15.72 cm in males (considered to be one year old).

• Fisheries and Aquatic Sciences
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• v.7 no.3
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• pp.136-140
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• 2004

We examined the ovaries of 312 robust tonguefish (Cynoglossus robustus) collected each month from January to December 2001, off the coast of Yeosu, Korea. The samples were used to investigate robust tonguefish reproductive activity. Monthly changes in the gonadosomatic indexindicated that spawning peaked between June and August. Fish size at first spawning was 15-19 cm total length (TL). Over $50{\%}$ of female fish 25-29 cm TL were sexually mature, and this rose to $100{\%}$ for female fish 35-39 cm TL. The tonguefish spawned four or more times during the spawning season. Most females commenced spawning in their second year. The relationship between total length (cm TL) and fecundity (F) was F=528646Ln(TL)-1E+06.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.2
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• pp.188-192
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• 2018

The reproduction characteristics of hagfish Eptatretus burgeri were examined using individuals caught in the South Sea of Korea. The spawning season and size at minimum sexual maturity of this species were characterized based on a gonad-somatic index (GSI) and monthly variation egg size (long axis). From monthly variation of GSI, the spawning season was estimated to be from August to September. Developing eggs larger than 10 mm were found in March, and the largest egg size was found in July. The first spawning length was 34.2 cm TL. Batch fecundity ranged from 13 to 117 eggs for hagfish sized from 34.2 cm TL to 77.0 cm TL, respectively, and increased linearly with total length.