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Identification of Molecular Markers Linked to Ti Locus in Soybean

  • Kim Myung Sik;Park Min Jung;Hwang Jung Gyu;Jo Soo Ho;Ko Mi Suk;Chung Jong Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.5
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    • pp.419-422
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    • 2004
  • Soybean is a major source of protein meal in the world. Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to identify RAPD markers linked to KTI protein allele using bulked segregant analysis. Cultivar Jinpumkong2 (TiTi) was crossed with C242 (titi, absence of KTI protein) and F. seeds were planted. The $F_1$. plants were grown in the greenhouse to produce $F_2$ seeds. Each $F_2$ seed from $F_1$. plants was analysed electrophoretically to determine the presence of the KTI protein band. The present and absent bulks contained twenty individuals each, which were selected on the basis of the KTI protein electrophoresis, respectively. Total 94 $F_2$ individuals were constructed and 1,000 Operon random primers were used to identify RAPD primers linked to the Ti locus. The presence of KTI protein is dominant to the lack of a KTI protein and Kunitz trypsin inhibit protein band is controlled by a single locus. Four RAPD primers (OPAC12, OPAR15, OPO12, and OPC08) were linked to the Ti locus. RAPD primer OPO12 was linked to Ti locus, controlling kunitz trypsin inhibitor protein at a distance of 16.0 cM. This results may assist in study of developing fine map including Ti locus in soybean.

A Study on the Sediment Flux in Gwangyang Bay during Spring Tide, March 2003 (2003년 3월 대조기 광양만 부유퇴적물의 유 · 출입에 관한 연구)

  • KIM Seok Yun;LEE Byoung Kwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.6
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    • pp.511-516
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    • 2004
  • As a preliminary study on the sediment flux, concentrations of suspended particulate matter and current speeds were measured at three inlets of Gwangyang Bay during one tidal cycle of a spring tide of March 2003. The suspended sediment flux rate $(g/m^{2}/s)$ at the mouth of Seomjin River (St. K1) was observed to be higher throughout surface layer during ebb tide $(14.3\;g/m^{2}/s)$ and throughout near-bottom layer during the flood tide $(23.2\;g/m^{2}/s),$ resulting in a net upstream-ward transport of$0.9{\times}10^{3}kg/m$ during 13 period. At the inlet toward Yeosu Bay (St. K2), a relatively low rate ($(5.0-6.7\;g/m^{2}/s)$ of sediment flux occurred throughout the water column compared to St. K1, with a depth-integrated net transport of $5.6{\times}10^{3}kg/m$ toward the outer reaches of Gwangyang Bay inlet. At St. K3 located at Gwangyang Bay-side of Noryang Strait, the outward flux toward the Jinju Bay was observed to be dominant during the flood tide $(16.2-23.2\;g/m^{2}/s)$, especially through the mid and near bottom layer, compared to the inward flux throughout the whole water column during the ebb tide $(13.1-19.7\;g/m^{2}/s).$ The net transport at St. K3 was calculated to be $4.0{\times}10^{3}kg/m$ toward the outside of Gwangyang Bay. The outward net transport of suspended sediment at all three inlets seems to be consistent with a trend of bottom sediment texture, which suggests a net movement of sediment from a relatively coarse and poorly sorted inner-bay toward a relatively fine and better sorted outer-bay environment.

Spatial Distribution of Benthic Macroinvertebrates at Three River Weirs in The Namhan River (남한강 본류 3개 보의 저서성 대형무척추동물의 공간적 분포)

  • Kown, Yongju;Kim, Jin-Young;Kim, Piljae;Kim, Jungwoo;Kim, Jeong-KI;Kong, Dongsoo
    • Journal of Korean Society on Water Environment
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    • v.36 no.1
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    • pp.36-47
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    • 2020
  • Three large scale weirs were constructed 2010 - 2011 in the Namhan river, Korea. The purpose of this study was to investigate the spatial distribution of benthic macroinvertebrates and the influence of environmental factors at the weirs 2014 - 2015. The number of species was higher in the riparian zone than in the transition or the limnetic zone. This seems to be because of the diversification of microhabitats and food sources according to the development of littoral zones. From the riparian zone to the limnetic zone, the individual abundance proportion of gathering collectors among functional feeding groups decreased, and that of filtering collectors increased. In the limnetic zone, sprawlers and climbers among habitat orientation groups decreased, and burrowers increased. This means that coarse particulate organic matter originated from land or riparian zone was transformed to fine particulate organic matter in the limnetic zone. Asian clam (Corbicula fluminea) and chironomids were dominant species based on individual abundance. Asian clam, a major taxon considering biomass, was abundant toward the limnetic zone. This is becasue of the shallow depth, suitable water current, slightly coarse substrate, and good water quality. There was no significant relationship between the water quality and the characteristics of the benthic macroinvertebrate community because the water quality was spatially not heterogenous. The more influential factors for benthic community were physical factors, especially water depth. Water depth showed a markedly significant correlation with Shannon-Weaver's species diversity (r=-0.90), Margalef's species richness (r=-0.82), and McNaughton's dominance (r=0.86). Water depth showed a positive correlation (r=0.68) with the Kong and Kim BMSI (Bentic Macroinverebrates Streambed Index), and this may be related to the coarse substrate of the limnetic zone.

The distribution characteristics of trace elements of fine ambient particulate in Korea

  • Lim, Jong-Myoung;Lee, Jin-Hong;Kim, Ki-Hyun
    • Analytical Science and Technology
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    • v.18 no.3
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    • pp.241-249
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    • 2005
  • In this study, instrumental neutron activation analysis was used to measure the concentrations of about 30 trace elements in airborne particulate (PM2.5) samples at urban area of Daejeon city located in the southwestern region of Korea. An inspection of the measurement data indicates that the distribution patterns of trace elements could clearly distinguish the elements with their concentrations ranging over five orders of magnitude. The mean values for Lu and Dy were found to be the lowest at values of 0.01 and $0.04ng/m^3$, while those for K and Fe showed the highest value of 671 and $653ng/m^3$, respectively. The results of correlation analysis showed that PM2.5 concentrations can exhibit much more enhanced correlations with the elements of earth crustal components. The results of factor analysis further indicated that there are no more than six factors with statistical significance, which may exert dominant roles in regulating the elemental concentration levels in the study area. Enrichment factor analysis supports explicit interpretation of results found by this factor analysis.

Punching Fracture Simulations of Circular Unstiffened Steel Plates using Three-dimensional Fracture Surface (3차원 파단 변형률 평면을 이용한 비보강 원판의 펀칭 파단 시뮬레이션)

  • Park, Sung-Ju;Lee, Kangsu;Choung, Joonmo
    • Journal of Ocean Engineering and Technology
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    • v.30 no.6
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    • pp.474-483
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    • 2016
  • Accidental events such as collisions, groundings, and hydrocarbon explosions in marine structures can cause catastrophic damage. Thus, it is extremely important to predict the extent of such damage, which determines the total amount of oil spills and the residual hull girder strength. Punching fracture tests were conducted by Choung (2009b), where various sizes of indenters and circular unstiffened steel plates with different thicknesses were used to quasi-statically realize damage extents. A three-dimensional fracture strain surface was developed based on a reference (Choung et al., 2015b), where the average stress triaxiality and average normalized Lode angle were used as the parameters governing the fracture of ductile steels. In this study, new numerical analyses were performed using very fine axisymmetric elements in combination with an Abaqus user-subroutine to implement the three-dimensional fracture strain surface. Conventional numerical analyses were also conducted for the tests to identify the best fit fracture strain values by changing the fracture strains. Based on the phenomenon of the average normalized Lode angle starting out positive and then becoming slightly negative, it was inferred that the shear stress primarily dominates in determining the fractures locations, with a partial contribution from the compressive stress. It should be stated that the three-dimensional fracture surface effectively predicted at least the shear stress-dominant fracture behavior of a mild steel.

Evaluation of the Effect of Aggregate Structure on Rutting Performance of Asphalt Pavement (아스팔트 포장의 소성변형에 대한 골재 구조의 영향 평가)

  • Kim, Boo-Il
    • International Journal of Highway Engineering
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    • v.9 no.4
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    • pp.135-147
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    • 2007
  • Segregation in asphalt pavements occurs as a result of the non-uniform distribution of coarse and fine aggregates and causes premature distresses, such as cracking, raveling, and stripping. The effect of segregation on rutting, however, has not been clearly identified. Experimental and analytical work performed in this study indicates that rutting performance is affected by segregation of mixtures. However, the aggregate structure of mixtures appears to be a more critical factor that determines the rutting performance, rather than the level of segregation. Based on the field mixtures evaluated, an increase of coarse aggregate volume in an asphalt mixture is an important factor that results in good rutting performance. This effect holds true for mixtures with lower levels of air voids, but for mixtures with higher levels of air voids, the air voids effect becomes dominant, resulting in a reduction in rutting performance. An air void content of 10% appears to be a threshold that determines the rutting performance of Superpave mixtures. Once the air void content exceeds 10%, the rutting performance of Superpave mixtures decreases significantly, despite the coarse aggregate volume.

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Electron beam weldability of Niobium (니오븀의 전자빔 용접성)

  • An, Byung-Hun;Yoon, Jong-Won;Kim, Sook-Hwan
    • Laser Solutions
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    • v.11 no.2
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    • pp.15-19
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    • 2008
  • Electron beam (EB) weldability of pure grade Nb sheet was studied. One of Nb sheets was as-annealed and the other was cold rolled. Microstructures, Vickers hardness, and transverse weld tensile test were carried out for the base metal, the heat affected zone (HAZ) and weld metal. In the case of the EB welds made using the annealed Nb sheeet, fine equiaxed grains and coarse grains were dominant at the base metal and the HAZ, respectively, and columnar grains were observed at the weld metal. For the EB welds made using the cold rolled Nb sheet, elongated grains in the rolling direction at the base metal, and the microstructures of the weld metal and the HAZ are similar to those of the EB welds made using the annealed Nb sheet, respectively. For both annealed and cold rolled Nb sheet, the width of the HAZs are unusually wide in spite of using high density heat source, i.e. electron beam, and the grain sizes of both HAZs are similar. When tensile test was carried out using the transverse weld specimens, the failure occurred at the HAZ for both EB welds made using Nb sheets annealed and cold rolled, respectively and the tensile strengths of both specimens were 161MPa. Vickers hardness of EB welds made using annealed Nb was 56-57 Hv at both base metal and weld metal, 52-53 Hv at the HAZ. On the other hand, Vickers hardness of EB welds made using cold rolled Nb was 97-99 Hv at the base metal, but the hardness values of weld metal were similar to those obtained at the weld metal of annealed Nb.

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Spatio-temporal Variation and Evaluation of Benthic Healthiness of Macrobenthic Polychaetous Community on the Coast of Ulsan (울산 연안 해역 저서다모류 군집의 시·공간 변동 및 저서건강도 평가)

  • Jeong, Bong Geun;Shin, Hyun Chool
    • Ocean and Polar Research
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    • v.40 no.4
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    • pp.223-235
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    • 2018
  • This study was carried out to investigate benthic sedimentary environments and benthic polychaetous communities on the coast of Ulsan, located on the southern East Sea of Korea. This survey was conducted at 15 stations, four times seasonally in January, April, July and October 2016. From the coast to the outer sea, surface sediments turned into fine grained sediments. There were complex coarse-grained sedimentary facies in various forms in the coastal zones while those with mud facies were found in the offshore zone. Organic matter content (LOI) and sulfide amount (AVS) recorded extremely high values, and increased from the coast to the outer sea, showing a similar trend to mud content with depth. The benthic polychaetous community revealed a mean density of $525ind./m^2$, and the total species number of species was 84. The major dominant polychaetous species were Magelona japonica, Lumbrineris longifolia and Heteromastus filiformis throughout the four seasons. Magelona japonica was concentrated predominantly in shallow coastal areas, but was present in all the regions of the survey area. Lumbrineris longifolia showed higher density in offshore regions more than 30 m deep, whereas H. filiformis showed higher density in coastal areas less than 30 m in depth. As a result of cluster analysis, the study area was divided into three ecological areas according to species composition, such as the northern coastal area between Ulsan PortOnsan Port, the southern area around Hoeya River and the outer sea area. Benthic environments in the study area, as determined by AMBI and BPI index, maintained a healthy condition in all four seasons with the AMBI at a level above GOOD and BPI at a level above FAIR. As organic matter accumulation continues to take place in the Ulsan coastal area, it is essential that detailed research activities continue to be carried out and ongoing monitoring be maintained.

Effects of Solution Treatment Temperatures on Microstructure and Mechanical Properties of TIG-MIG Hybrid Arc Additive Manufactured 5356 Aluminum Alloy

  • Zuo, Wei;Ma, Le;Lu, Yu;Li, Shu-yong;Ji, Zhiqiang;Ding, Min
    • Metals and materials international
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    • v.24 no.6
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    • pp.1346-1358
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    • 2018
  • A novel additive manufacturing method with TIG-MIG hybrid heat source was applied for fabricating 5356 aluminum alloy component. In this paper the microstructure evolution, mechanical properties and fracture morphologies of both as-deposited and heat-treated component were investigated, and how these were affected by different heat-treated temperature. The as-deposited microstructure showed dominant equiaxed grains with second phase, and the size of them is coarse in the bottom region, medium in the middle region and fine in the top region owing to different thermal cycling conditions. Compared with as-deposited microstructure, the size of grain becomes large and second phases gradually dissolve in the matrix as heat-treated temperature increase. Different microstructures determine the mechanical properties of component. Results show that average ultimate tensile strength enhances from 226 to 270 MPa and average microhardness increases from 64.2 to 75.3 HV0.1 but ductility decreases from 33 to 6.5% with heat-treated temperature increasing. For all components, the tensile properties are almost the same in the vertical direction (Z) and horizontal direction (Y) due to equiaxed grains, which exhibits isotropy, and the mechanisms of these are analyzed in detailed. In general, the results demonstrate that hybrid arc heat source has the potential to fabricate aluminum alloy component.

Analysis of Consumer Preference on Mid to Long Term Power Sources by Using a Choice Experiment (선택실험법을 이용한 중장기 전원별 소비자 선호 분석)

  • Jung, Heayoung;Bae, Jeong Hwan
    • Environmental and Resource Economics Review
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    • v.27 no.4
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    • pp.695-723
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    • 2018
  • Recently, extreme weather due to climate change has become more frequent, and increase of fine dust has worsen air quality in Korea. Therefore, not only negative perception on coal-fired power generation is dominant, but also the social acceptance of nuclear power generation declines. This study aims at deriving consumer preferences on the mid and long term power mix with various energy sources. Willingness to pay for each generation source was estimated and the preference heterogeneity of consumers was examined by using mixed logit and latent class models. Mixed logit estimation results show that the preference heterogeneity of consumers is especially large for the nuclear power relative to renewable or coal energy. According to the estimation results from the latent class model, group 1 prefers renewable energy while group 2 prefers coal energy. Group 3 shows lexicographic preference which means restricted rationality. As for the policy implication, it is necessary to understand the preference heterogeneity of consumer groups in planning the mid to long term power mix.