• 한국임상수의학회지
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• 제6권2호
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• pp.291-305
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• 1989

Present experiment was performed to establish the optimal reaction conditions for measurement of urinary gamma-glutamyltranspeptidase(${\gamma}$-GTP), N-acetyl-${\beta}$-D-glucosaminidase (AGS) and alanine aminopeptidase(AAP) activities in bovine and to investigate in vitro stability of the enzymes, within-run imprecision of the methods, and normal ranges. 1. The optimal wavelength for measurement of ${\gamma}$-GTP activity was 545nm. 2. The optimal pH of Tris-HCI buffer containing glycylglycine for measurement of urinary ${\gamma}$-GTP activity was 7.6～7.8(37$^{\circ}C$). 3. Coefficient of variance for within-run imprecision of urinary ${\gamma}$-GTP activity ranged from 4.8 to 7.2% and there was no significant difference among replications, 4. The optimal wavelength for measurement of urinary AGS activity was 405nm. 5. The optimal pH of citrate buffer for measurement urinary of AGS activity was 4.0(37$^{\circ}C$). 6. Coefficient of variance for within-run imprecision of urinary AGS activity ranged from 3.9 to 6.1％ and there was no significant difference among replications. 7. The optimal wavelength for measurement of urinary AAP activity was 400nm. 8. The optimal pH of phosphate buffer for measurement of urinary AAP was 7.8. 9. Coefficient of variance for within-run imprecision of urinary AAP activity ranged from 2.5 to 4.8% and there was no significant difference among replications. 10. ${\gamma}$-GTP and AGS activities were increased significantly by gel-filtration. 11. Turbidity interfered with measurement of urinary AAP activity in bovine unless the specimen was gel-filterated. 12. Preservation of the specimen at 5$^{\circ}C$ or -20$^{\circ}C$ did not affect the AGS activity at least for 7 days after collection. 13. Preservation of the specimen at 5$^{\circ}C$ or 20$^{\circ}C$ did not affect the ${\gamma}$-GTP and AAP activities statistically, but some individual specimens revealed fluctuation during preservation. 14. ${\gamma}$-GTP, AGS and AAP activities revealed fluctuation by the tine of the day when the specimen was collected. 15. The normal ranges of urinary ${\gamma}$ -GTP, AGS and AAP activities were 6.60${\pm}$3.26(2.36-14.50), 1.31 ${\pm}$ 0.81(0.33-3.78), and 1.73 ${\pm}$ 0.55(0.77-3.03)U/l. respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1268-1273
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• 2011

The present study was conducted to determine a feasible method of protein concentrate extraction from rice bran (RBPC) and its effect as a substitution for skim milk in early weaning pig diets. An investigation to extract protein concentrate from full fat rice bran was undertaken to determine the best ratio of water and rice bran, the amount of NaOH and a HCl solvent to use in a simple paddle-type mixer with modified spinning to produce RBPC. The results stated that the best ratio for water mixing in the RBPC extraction process was 1:5 with 20 g NaOH and 30 min in a paddle-type mixer at 300 rpm. A mix of 250 ml 0.2 N HCl was optimum for neutralization and protein precipitation. After the fluid was spun out with a washing machine, the sediment was left for 12-14 hours to complete the filtration. One kilogram of rice bran could produce an average of 324.5 gram RBPC and it contained 3.40% ash, 496.48 kcal of GE/100 gram, 1.94% crude fiber, 28.20% ether extract, 7.64% moisture and 16.66% crude protein, respectively. A total of 45 crossbred piglets, weaned at 3 weeks of age were allotted into control diet (A) and dietary treatments formulated with a four different rates of RBPC substitution for skim milk at a percentage of 25 (B), 50 (C), 77 (D) and 100 (E) respectively, in a randomized complete block (RCB) design. All piglets had free access to feed and water until 8 week of age when the experiment ended. Feed intake, average daily gain, growth rate and feed efficiency were not affected by dietary treatments. Blood test parameters after completion of the growth trial indicated normal health. Even though the mean of cell hemoglobin concentration was significantly different between treatments (p<0.05) it was still within the normal range. The cost difference for BW gain of 100% RBPC substituted for skim milk in the weaning diet was approximately 35% lower than that of the control and the relative cost of production was 96.67, 92.85, 70.75 and 64.48% lower for the replacement of 25, 50, 75 and 100% of skim milk respectively. These results implied that this technology is feasible for use by small scale farmers to improve their self-reliance.

• 한국작물학회지
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• 제48권3호
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• pp.184-190
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• 2003

국산 맥주보리의 품질향상을 위한 육종을 도모하기 위하여 농촌진흥청 영남농업시험장에서 보유하고 있는 맥주보리 유전자원 59품종 및 계통을 중심으로 원맥 및 맥아의 품질분석을 실시하여 품질이 우수한 자원을 선발하여 품질향상을 위한 육종소재로 이용할 목적으로 수행하였다. 1. 원맥의 단백질함량은 평균 12.3% (10.7-14.9%)를 나타내었으며, $\beta$-글루캔함량은 3.5-5.8%로 평균 4.6%였다. 2. 맥아신장도, 불발아율, Friability, 여과시간, 맥즙추출율, 콜박지수 및 효소력가는 품종간 유의 차가 인정되었으나, 그 밖의 형질은 차이가 없었다. 3. 15개 품질관련 형질간의 상관분석 결과, 원맥의 단백질 함량이 높을수록 맥아 단백질($0.800^{**}$)은 상대적으로 증가하지만, 당도(-$-0.578^{**}$). Friability($-0.554^{**}$), 맥즙추출율($-0.592^{**}$) 및 콜박지수($-0.681^{**}$)등은 낮아져 품질을 저하시키는 고도의 부의 상관이 인정되었다. 4. 맥아의 단백질함량이 높아짐에 따라서 추출을($-0.774^{**}$)과 콜박지수($-0.778^{**}$)가 현저하게 감소되는 등 품질저하의 원인이 되었다. 5. 본시험에서 추출율이 80% 이상이면서 콜박지수 및 효소력가 등 종합적으로 품질이 우수한 품종 및 계통은 외국품종으로서 Viva, Nishino chikara, Kinukei 9 및 Kinukei 12 의 4품종 및 계통과 국내육성 품종으로서 사천6호와 진양보리였다

• 대한환경공학회지
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• 제29권7호
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• pp.783-792
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• 2007

부직포 여과막 생물반응조의 질산화 성능을 파악하기 위하여 주입폐수의 암모니아 농도를 $54\sim1,400$ mg/L 그리고 알칼리도를 $43\sim10,480$ mg/L로 변화시키면서 약 11시간의 체류시간에서 641일간 실험을 실시한 결과 반응조의 MLSS농도는 최초의 2,650 mg/L 에서 830 mg/L까지 감소하였다가 최고 8,340 mg/L까지 증가함으로써 반응조의 용적부하는 $0.120\sim3.130$ kg $NH_3-N/m^3-day$의 범위에서 변하였으나 F/M 비는 $0.067\sim0.414$ kg $NH_3-N/kg$ MLSS-day의 적은 변화를 보였다. 각 실험단계별 평균 질산화 효율이 $35.2\sim100%$로서, 최대 질산화율은 2.970 kg $N/m^3-day$ 또는 0.489 g N/g MLVSS-day로 나타났다. MLVSS의 질산화미생물 분율은 최초의 7.1%에서 최고 100%까지 변하였으나 부직포 여과막에 형성된 생물막의 경우에는 2.2%의 매우 낮은 값을 보였다. 미생물 성장계수는 0.117 g VSS/g N removed로 그리고 알칼리도 소모량은 평균 7.08 g alkalinity/g NOx-N produced로 측정되었다. 이러한 실험결과로 보아 부직포 여과막 생물반응조가 고농도 암모니아 폐수의 질산화에 적합한 공법으로 판단된다.

• 대한환경공학회지
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• 제30권1호
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• pp.45-53
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• 2008

본 연구에서는 국내 한 정수장의 고도정수처리공정의 실험분석 자료를 바탕으로 정수장으로 유입되는 원수의 수질특성과 처리공정별 오염물질 제거효율의 변화특성을 연구하였고, 활성탄여과조의 역세척에 따른 BAC 내의 미생물 거동을 추적하였다. KMnO$_4$ 소비량 등 8$\sim$9개의 주요 항목을 중심으로 제거특성이 연구되었고, 활성탄여과조의 유입 유출수에 대하여 NH$_3$-N 등 4항목의 농도변화를 추적하였다. 역세척 전 후의 활성탄 시료의 SEM 사진을 촬영하였고, 미생물 생체량(HPC) 조사를 통해 활성탄 부착미생물의 존재를 확인하고자 하였다. 유입원수의 특성은 대부분의 조사항목이 일정한 농도범위로 유입되었고 그 중 계절적 일관성을 갖는 항목이 있었다. 공정별 제거효율 변화특성은 월별 일정한 유형을 갖는 것, 공정진행에 따라 지속적으로 제거되는 것, 오존접촉조에서 제거효율이 떨어지는 것 등으로 분류되었고, 현재 운전되고 있는 고도처리공정에서 제거되는 주요 대상오염물질을 구별할 수 있었다. NH$_3$-N의 농도변화 곡선으로 활성탄여과조 내에 미생물이 존재함을 예측하여 SEM 사진과 HPC 측정으로 이를 확인하였다.

• 상하수도학회지
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• 제9권3호
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• pp.116-126
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• 1995

The purpose of this study was to investigate the removal of ammonium nitrogen by biological nitrification in raw water containing LAS using BAC. At batch teats, LAS removal by ozone followed the first order reaction, and the rate constants(k) by ozone dose 1, 3mg/min.L were $0.040min^{-1}$, $0.062min^{-1}$ respectively. Therefore, the more ozone was dosed, the higher LAS was removed The reaction between ozone and ammonium nitrogen also followed the first order, and rate constants(k) at pH7,8 and 9 were $8.9{\times}10^{-4}min-1$, $3.8{\times}10^{-3}min^{-1}$, and $2.9{\times}10^{-2}min^{-1}$ respectively at ozone dose of 3mg/min.L . Therefore, ammonium nitrogen was little removed by ozone under neutral pH of 7. The continuous flow apparatus had four sets composed of a ozone contacter and a GAC column. Through continuous filtration test for 50days, the following conclusions were derived; (1) LAS was removed 23%, 30% respectively by ozone dose 1, 3mg/L, and was not detected in all column effluents during the period of experiment. Therefore, it appeared that adsorption capacities of each column still remained. (2) Ammonium nitrogen concentration after ozone contact varied little in raw Water because pH of raw water was from 6 to 7, and was transfered to nitrite and nitrate within GAC columns as the result of staged nitrification. After 30days, nitrite was not detected in all column effluents due to biological equilbrium between nitro semonas and nitrobacter Average removals of ammonium nitrogen in each column after the lapse of 30days were the following; ${\cdot}$ column A (ozone dose 3mg/L, EBCT 9.5min): about 100% ${\cdot}$ column B (ozone dose 1mg/L, EBCT 9.5min): 91% ${\cdot}$ column C (ozone dose 3mg/L, EBCT 14.2min): about 100% ${\cdot}$ column D (ozone dose 0mg/L, EBCT 9.5min): 53% Though column A and C reached nitrification of about 100%, column C (longer EBCT than column A) was more stable than column A. (3) After backwash, nitrification reached steady state within 5 to 8 hours. Therefore, nitrification was not greatly affected by backwash. (4) According to the nitrification capacity in depth of column A, C, where 100% nitrification occured. LAS was removed within 20cm, while ammonium nitrogen required more depth to be removed by nitrification.

• 약학회지
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• 제34권4호
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• pp.238-243
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• 1990

Pharmacokinetic characteristics of recombinant human interleukin-2 (rH IL-2) wre studied in the rat. First, different doses of rH IL-2 ranging from 6,400 to 1,600,000 U/kg were injected intravenously and the effect of dose size on the pharmacokinetics was examined. There was no dose dependency in the pharmacokinetics of rHIL-2 in the dose range of 6,400-40,000 U/kg. But at the dose of 1,600,000 U/kg, there was a severe hemolysis throughout the experiment and the pharmacokinetic parameters such as Vdss and CLt were significantly increased compared to those obtained from lower doses. It also showed that this drug is hardly distributed to the peripheral tissues and hardly eliminated from the body, since the valume of distribution (Vdss) and total body clearance (CLt) were 45-75 ml/kg and 1-2 ml/min/kg, respectively. The Vdss is close to the actual plasma volume and the CLt is less than glomerular filtration rate (GFR). Therefore it seemed that rH IL-2 is distributed only in the plasma pool and hardly filtered in the kidney due to its very large molecular weight. Second, rH IL-2 was administered to the rat via several routes such as hepatic portal vein (PV), intraperitoneal (IP), peroral (PO) and intranasal (IN) routes. The bioavailabilities (BA) of PV, IP, PO and IN routes were 96.8, 4.9, 0 and 0.1%, respectively. The addition of some nasal absorption enhancers such as taurocholate, taurodeoxycholate, glycocholate and glycodeoxycholate did not increase the BA of intranasaly administered rH IL-2. The result is contrast to the effect of these bile salts on the nasal absorption of ${\alpha}-inteferon$. Considering it together with the pharmacokinetic parameters, very large molecular weight of rH IL-2 seemed again to be the cause to very poor membrane permeability.

• 한국환경농학회지
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• 제28권3호
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• pp.227-232
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• 2009

돈분액비와 같은 가축분 퇴비의 공급은 유용한 유기 자원으로서 뿐만 아니라 작물에 필요한 영양을 공급하는 역할로서 알려져 있다. 하지만, 돈분 액비의 연용에 따른 작물 재배 대한 연구 결과의 보고는 많지 않다. 본 연구의 목적은 4작기 (2007-2008) 배추 연용 토양에서 SCB액비 처리에 대한 토양화학적 특성의 변화 및 수량에의 영향을 화학 비료구를 대조구로 하여 평가하고자 하였다. 시험작물은 봄작기에는 노랑 봄배추를 가을작기에는 노란자배추를 이용하였으며 정식 후 수확까지의 재배일은 연도순으로 각각 봄작기에는 58, 50일이었으며 가을작기에는 72, 75일 기간동안 재배하였다. SCB 액비의 시용량은 토양검정 후 질소요구량에 대하여 액비 중의 총 질소 함량을 기준으로 매 작기별 해당량을 시용하였고 추비는 화학비료로 하여 공급하였다. 비료의 시비비율은 35% : 65%의 비율로 공급하였으며 대조구인 화학비료구도 동일한 비율로 처리하였다. 4작기 동안에의 배추 수량에 있어서 SCB 액비와 화학비료와의 수량의 차이는 없었으며 또한 배추의 당 및 비타민 C 함량 비교 분석에서도 통계적인 유의성은 없었다. 양분이용효율 및 토양 중 미량원소 등에서도 처리간의 차이는 보이지 않았으나, 토양양분함량의 변화에 있어서 액비의 2회 연용 시부터 치환성 나트륨 함량의 증가를 보였다. 이러한 결과는 밑거름으로 추천된 질소량에 대한 SCB 액비의 다년간의 시용은 수량은 만족하지만 토양 중의 치환성 나트륨 함량을 증가시킨다고 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.896-902
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• 2015

Milking center wastewater (MCW) has a relatively low ratio of carbon to nitrogen (C/N ratio), which should be separately managed from livestock manure due to the negative impacts of manure nutrients and harmful effects on down-stream in the livestock manure process with respect to the microbial growth. Simultaneous nitrification and denitrification (SND) is linked to inhibition of the second nitrification and reduces around 40% of the carbonaceous energy available for denitrification. Thus, this study was conducted to find the optimal operational conditions for the treatment of MCW using an attached-growth biofilm reactor; i.e., nitrogen loading rate (NLR) of 0.14, 0.28, 0.43, and $0.58kg\;m^{-3}\;d^{-1}$ and aeration rate of 0.06, 0.12, and $0.24\;m^3\;h^{-1}$ were evaluated and the comparison of air-diffuser position between one-third and bottom of the reactor was conducted. Four sand packed-bed reactors with the effective volume of 2.5 L were prepared and initially an air-diffuser was placed at one third from the bottom of the reactor. After the adaptation period of 2 weeks, SND was observed at all four reactors and the optimal NLR of $0.45kg\;m^{-3}\;d^{-1}$ was found as a threshold value to obtain higher nitrogen removal efficiency. Dissolved oxygen (DO) as one of key operational conditions was measured during the experiment and the reactor with an aeration rate of $0.12\;m^3\;h^{-1}$ showed the best performance of $NH_4-N$ removal and the higher total nitrogen removal efficiency through SND with appropriate DO level of ${\sim}0.5\;mg\;DO\;L^{-1}$. The air-diffuser position at one third from the bottom of the reactor resulted in better nitrogen removal than at the bottom position. Consequently, nitrogen in MCW with a low C/N ratio of 2.15 was successfully removed without the addition of external carbon sources.

• Ecology and Resilient Infrastructure
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• 제3권4호
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• pp.247-255
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• 2016

강우 시 표면 유출에 의한 비점오염물질의 하천 유입은 도시 하천 관리에 부정적인 영향을 미치며 특히 강우 초기의 수질농도가 매우 높은 것으로 알려져 있다. 본 연구에서는 하천의 제외지 또는 유수지 등의 공간을 활용하여 침강 및 여과작용을 이용하여 최소한의 에너지 및 비용을 이용하여 오염물질을 정화시키면서 하천의 수질 및 수량 관리에 긍정적인 작용을 할 수 있는 장치와 시스템을 개발하고자 하였다. 빗물 자동 모니터링 시스템을 이용하여 초기 우수에 대한 유량 및 수질을 파악하였고 이를 토대로 처리 시스템의 설계 기준을 확립하고자 하였다. 초기우수 제어 장치는 침전 장치만으로도 총부유물질 (TSS) 84 - 95%, 총질소 (TN) 31 - 46%, 총인 (TP) 42 - 86%의 제거 효율이 나타났다. 또한 일기 예보를 통해 강우에 의한 유량 및 수질을 예측하고 처리장치의 상태 및 자동 모니터링시스템과 연계하여 시설을 종합적으로 관리할 수 있는 강우 유출 종합관리 시스템 ISTORMS (Integrated Stormwater Runoff Management System)를 개발하였다. 본 연구 결과는 도시 하천의 수질 및 유량 환경개선에 효율적으로 적용될 수 있을 것으로 기대된다.