• 대한치과교정학회지
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• 제29권5호
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• pp.535-549
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• 1999

본 연구는 Contraction Utility Arch wire를 이용하여 성견의 상악 4절치에 후방 견인력과 압하력을 2주와 4주간 적용하여 치아 및 치주 조직의 조직학적 변화를 관찰하여 생체에서 나타나는 생물학적인 효과와 작용의 진행과정을 확인하고자 시행되었다. 생후 약 10개월 정도의 성견 3 마리를 각각 실험군 2마리와 대조군 1 마리로 나누어 제 1, 2 절치에 1mm의 후방 견인력과 60gm의 압하력이 가해지도록 Contraction UTA를 삽입하였다. 장치 부착없이 2주후 희생시킨 것을 대조군으로, 장치 장착 2주후 희생시킨 것을 실험 1군으로, 장치 장착후 재활성화없이 4주후 희생시킨 것을 실험 2군으로 하여 각 군의 제 1, 2 절치에 대한 탈회 표본을 제작하여 Hematoxylin-Eosin염색과 Masson's Trichrome염색을 시행한 후, 각 치아의 치경부 l/3, 치근 중앙 1/3, 치근단 1/3의 치근 및 치아 주위 조직을 광학 현미경 하에서 관찰하여 다음의 결론을 얻었다. 1. 대조군 소견에서, 치주 인대는 치근단 부위에서 치조정까지 일정한 형태를 이루며 비교적 균일한 두께와 연속성을 나타내고 있었으며, 치조골 표면은 조골 세포와 함께 부드러운 골면을 이루고 있었다. 치근의 중앙부 및 치근단 부위에서는 세포성 백악질의 침착을 보였다. 2. 실험 1군의 제 1 절치에서, 치주 인대는 순설측 치경부에서 치근단 방향으로 신장되었다가 치근단부에서 압축되어 치조골에 평행하게 배열되는 소견을 보였으며, 치조골 흡수는 순측 치근 중앙부와 치근단부에서 활발히 진행되고 있었고 순측 치근단 부위의 치아면을 따라 백악질 흡수 부위가 산재되어 나타났다. 제 2 절치 역시 제 1 절치와 유사한 소견을 나타내고 있었으며 설측 치근단부에서 백악질 흡수 소와가 다수 관찰되었다. 3. 실험 2군의 제 1 절치에서,치주 인대의 주행 방향과 간격이 대조군 수준으로 회복되었으나, 치근단부에서는 여전히 혼재된 치주 인대 섬유의 주행이 관찰되었다. 설측 치근 중앙부에서 신생골의 석회화 반응이 강하게 관찰되었고, 순측 치근단부에서 골 흡수와 신생골 석회화가 진행중인 골 부위가 동시에 관찰되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권10호
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• pp.1366-1376
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• 2009

Effect of sulfur (S) on utilization efficiency of fresh cassava foliage and cassava hay in dairy cows was evaluated using thirty-two $1^{st}-2^{nd}$ lactation Holstein-Friesian crossbred dairy cows. The experimental treatment was a 2${\times}$2 factorial arrangement in a randomized complete block design (RCBD) using two roughages (rice straw+fresh cassava foliage (FCF) and rice straw+cassava hay (CH)) and two elemental sulfur (S) levels (0.15 and 0.4% S of dry matter (DM)), respectively. Four dietary treatments (FCF+0.15, FCF+0.4, CH+0.15 and CH+0.4) were offered ad libitum in the form of a total mixed ration (TMR) with concentrate to roughage (chopped rice straw+chopped cassava foliage) ratio at 60:40. Fresh cassava foliage or cassava hay resulted in similar dry mater intake, rumen ecology parameters, total tract digestibility, blood chemistry, milk production and composition. However, HCN intake, blood and milk thiocyanate concentration were significantly higher (p<0.01) in cows fed fresh cassava foliage with no sign of potential toxicity. Dry matter intake, body weight changes, molar percentage of propionate in rumen, neutral detergent fiber (NDF) digestibility and nitrogen (N) retention of cows tended to be increased while DM digestibility (65.6, 72.7, 68.6 and 72.1% of total DM intake for the respective treatments), rumen bacteria population (1.4, 1.7, 1.6 and $1.7{\times}10^{11}$ cell/ml for respective treatments), fungal zoospore population (0.4, 0.6, 0.4 and $0.5{\times}10^{6}$ cell/ml for respective treatments), urinary allantoin (25.3, 28.0, 26.3 and 27.6 g/d for respective treatments), microbial N yield (136.0, 154.6, 142.8 and 151.3 g N/d for respective treatments) and milk protein content (3.4, 3.5, 3.2 and 3.5% for respective treatments) were significantly (p<0.05) higher in cows fed on supplemented sulfur at 0.4% of DM in comparison with 0.15% S-supplemented diets. Based on these results, it is concluded that cassava foliage could be used as a portion of roughage for dairy cows and supplementation of S would be nutritionally beneficial.

• Asian-Australasian Journal of Animal Sciences
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• 제27권1호
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• pp.46-54
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• 2014

This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of $420{\pm}15.0$ kg, were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The dietary treatments were untreated rice straw (RS) without EuO (T1) and with EuO (T2) supplementation, and 3% urea-treated rice straw (UTRS) without EuO (T3) and with EuO (T4) supplementation. The EuO was supplemented at 2 mL/h/d in respective treatment. Experimental animals were kept in individual pens and concentrate mixture was offered at 3 g/kg BW while roughage was fed ad libitum. Total dry matter and roughage intake, and apparent digestibilites of organic matter and neutral detergent fiber were improved (p<0.01) by UTRS. There was no effect of EuO supplementation on feed intake and nutrient digestibility. Ruminal pH and temperature were not (p>0.05) affected by either roughage sources or EuO supplementation. However, buffaloes fed UTRS had higher ruminal ammonia nitrogen and blood urea nitrogen as compared with RS. Total volatile fatty acid and butyrate proportion were similar among treatments, whereas acetate was decreased and propionate molar proportion was increased by EuO supplementation. Feeding UTRS resulted in lower acetate and higher propionate concentration compared to RS. Moreover, supplementation of EuO reduced methane production especially in UTRS treatment. Protozoa populations were reduced by EuO supplementation while fungi zoospores remained the same. Total, amylolytic and cellulolytic bacterial populations were increased (p<0.01) by UTRS; However, EuO supplementation did not affect viable bacteria. Nitrogen intake and in feces were found higher in buffaloes fed UTRS. A positive nitrogen balance (absorption and retention) was in buffaloes fed UTRS. Supplementation of EuO did not affect nitrogen utilization. Both allantoin excretion and absorption and microbial nitrogen supply were increased by UTRS whereas efficiency of microbial protein synthesis was similar in all treatments. Findings of present study suggested that EuO could be used as a feed additive to modify the rumen fermentation in reducing methane production both in RS and UTRS. Feeding UTRS could improve feed intake and efficiency of rumen fermentation in swamp buffaloes. However, more research is warranted to determine the effect of EuO supplementation in production animals.

• 한국건축시공학회지
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• 제17권2호
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• pp.175-181
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• 2017

본 연구는 초고강도 섬유보강 콘크리트의 인장강도 특성을 파악하기 위한 일환의 연구로서 직접인장시험에 의한 노치가 도입된 시험체의 인장성능을 파악하기 위하여 시험변수는 목표 설계기준강도 120, 150 및 180MPa를 대상으로 하였으며, 양생조건을 일반 수중양생과 $90^{\circ}C$ 고온증기 양생조건으로 하여 그 특성을 검토하였다. 전반적으로 노치타입의 직접인장강도 시험체는 기존 직접인장 시험체에 비해 중앙균열 유도가 효과적인 것으로 나타났으며 데이터를 직접 인장강도-변형률 그래프로 나타낸 결과 먼저 재령 측면에서는 28일에서 56일로 진행할 때의 강도 상승이 가장 높은 것으로 측정 되었으며, 양생조건 측면에서는 고온증기 양생의 경우 수중양생의 비해 초기 강도가 높으나 장기 재령에 가까워질수록 두 가지 양생조건의 직접인장강도 차이가 미비해지는 것을 알 수 있었다. 최대인장강도는 수중양생의 경우 모든 목표설계강도가 재령이 증가할수록 일정하게 증가하는 것으로 나타났으며, 증기양생의 경우 재령 7일에서 초기강도 발현 효과로 인해 상당히 증가하는 경향을 나타내었다. 초기균열 강도는 수중양생의 경우 재령경과에 따라 증가하며, 증기양생의 경우 7일에서 수중양생에 비해 높은 것으로 나타난 반면에 28일 강도는 저하하는 경향을 나타내었다. 이부분에 대해서는 강섬유의 배열상태 등의 검토가 필요할 것으로 사료된다.

• Journal of Periodontal and Implant Science
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• 제26권3호
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• pp.735-751
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• 1996

An ultimate goal of periodontal therapy is to stop the disease process and to regenerate a functionally-oriented periodontium destroyed as a result of periodontal disease. The purpose of this study was to observe the effect of grafting granulation tissue obtained from extraction socket on the regeneration of horizontal furcation defect. Six dogs were used in this study. All mandibular first and third premolars were extracted. At 2, 3, and 5 days after extraction, tissues were obtained from extraction socket of 1 mongrel dog and examined by light microscope. Granulation tissue obtained at 5 days after extraction was chosen as the graft material. Five days later, horizontal furcation defects were created surgically at mandibular second and fourth premolars in the right and left side of the 5 beagle dogs. The entrance area of the artificially prepared "key hole" defects were about $3\;4mm^2$. By random selections, 2 exposed furcation defects were grafted with granulation tissue obtained from extraction socket as experimental group and 1 furcation defect was as control. The flaps were replaced to their original position and sutured with 4-0 chromic cat-gut. Three dogs were sacrificed 4 weeks and two dogs 8 weeks after surgery, and the prepared specimens were examined by light microscope. At 4 weeks, furcations were filled with epithelial lining and fibrous connective tissue infiltrated with chronic inflammatory cells. New bone formation was observed in all groups. Only experimental group showed new cementum formation. At 8 weeks, new cementum, functional arrangement of new PDL fiber, root resorption, and some ankylotic union of newly formed alveolar bone and root surface were observed in all groups. Experimental group showed that epithelial downgrowth was inhibited and new bone formation was more active compared to control. The success rate of the furcation defect healing was higher in experimental group than control. These results suggested that grafting of granulation tissue obtained from extraction socket which combined with reconstructive periodontal flap surgery may promote periodontal regeneration of horizontal furcation defect.

• Journal of the Korean Wood Science and Technology
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• 제29권1호
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• pp.60-67
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• 2001

소나무(Pinus densiflora)재의 1차조직과 2차조직의 조직구조, 미세구조 및 머서화(mercerization)에 의한 셀룰로오스의 결정구조 변화를 검토하였다. 그 결과, 1차조직의 세포는 원형에 가깝고 내강이 크며 배열이 무질서한 데 비하여, 2차조직의 세포는 장방형이고 비교적 규칙적인 배열을 갖고 있다. 섬유장은 1차조직 $200{\sim}250{\mu}m$, 2차조직 $1,500{\sim}1,600{\mu}m$였으며, 내강경은 1차조직 $40{\sim}50{\mu}m$, 2차조직 $10{\sim}20{\mu}m$였다. 그리고 1차조직과 1차방사조직은 2차조직의 세포에 비해 미목화된 세포가 많았다. 1차조직과 2차조직에서 셀룰로오스 결정의 면간격과 미결정 폭의 차이는 없었으나 상대결정화도는 각각 23%와 35%로 다소 차이가 있었다. 배향성에 있어서 1차조직의 미결정은 무배향을 나타냈으나 2차조직의 미결정은 섬유축에 $20{\sim}30^{\circ}$의 배향성을 나타냈다. 머서화 과정 동안에 1차조직 셀룰로오스 결정은 셀룰로오스 II로 변화되었지만 2차조직의 셀룰로오스 결정은 거의 변화되지 않았다. 따라서 양 조직간의 결정변태의 차이는 목화 정도의 차이에 의한 리그닌의 영향으로 생각되었다.

• Journal of Animal Science and Technology
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• 제44권3호
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• pp.341-350
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• 2002

본 시험은 한우수소 24두를 이용하여 성장단계별 증체량 및 건물 섭취량을 측정하고, 그에 대한 추정식을 유도하기 위하여 수행되었다. 시험구는 조사료 3종류(볏짚, 건초, 옥수수사일리지)와 조사료 급여비율 4수준(100, 60, 40 및 20%)의 3${\times}$4요인시험으로서 공시축은 incomplete turnover design에 의해 처리당 4두씩 배치되었다. 조사료의 종류 및 급여비율에 따른 한우수소의 일일 증체량은 옥수수사일리지 급여구 및 조사료 20% 급여구에서 각각 가장 많았다. 한우수소에게 건초 혹은 옥수수사일리지만 전량 급여하였을 경우에도 전 기간동안 증체가 지속되었지만, 볏짚구에서는 체중 350kg대 이후부터 체중이 감소하였다. 대사체중당 건물 섭취량은 체중이 증가할 수록 줄어들었으며, 조사료 공급원과 조사료 급여비율에 따른 건물 섭취량은 옥수수사일리지 및 조사료 20%구에서 각각 가장 높았다. 대사체중당($BWkg^{0.75}$) 건물 섭취량(DMI, kg/ day)에 따른 한우수소의 일일 증체량(DG, kg) 추정공식은 다음과 같이 유도되었다. DG = －0.842 + 17.5DMI/$BWkg^{0.75}$체중(BW, kg)과 일일 증체량(DG, kg)을 고려한 사료의 NDF 함량(NDF, %)과 건물 섭취량(DMI, kg/day)과의 관계식은 다음과 같이 유도되었다 DMI = 4.125 + 0.013BW + 1.075DG － 0.045NDF

• Journal of Periodontal and Implant Science
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• 제23권2호
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• pp.261-281
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• 1993

The author transplanted periodontally-diseased teeth which had been treated with citric acid into a clinically healthy extraction sockets and periodontally-affected extraction sockets, and compared with the healing processes within these tissues. Recipient sites were prepared by surgically removing a part of alveolar bone of premolars of adults dogs, placing elastic orthodontic ligatures for 8weeks, thereby inducing periodontal disease. The diseased roots were extracted and transplanted into healthy extraction sockets, and these were designated as control group 1. Diseased roots transplanted into diseased sockets were designated as control group 2. Diseased roots which had been root planed, treated with citric acid and transplanted into healthy sockets were designated as experimental group 1, while identically treated roots which had been transplanted into diseased sockets were designated as experimental group 2. Observations were made at weeks 2, 8 and 12, with following results. 1. At week 2, experimental group 2 showed some inflammatory cell infiltration in the connective tissue above the extraction sockets, while control groups showed less inflammatory or foreign body reactions throughout the experiment. 2. In both control groups, root surface resorption was observed throughout the experiment, while experimental groups showed a little resorption. 3. Control group 1 & 2 showed ankylosis by newly-formed bone ground the resorbed root surfaces, while experimental group 1 & 2 displayed collagen fibers which are not functionally-arranged, with random, loose arrangement or parallel orientation to root surfaces, and newly-formed bone outside of them. 4. In both control groups & experimental groups which had been transplanted into a clinically healthy extraction sockets & periodontally affected extraction sockets groups, histological differences were not significant. 5. Root resorption or ankylosis in control group 1 & 2 had increased quantitatively as experiment progressed. 6. New bone formation developed from the base and lateral wall of extraction sockets. In both control groups & experimental groups, root surfaces lying next to the upper portion of extraction sockets showed little alveolar bone formation and surrounded by connective tissue fiber at weeks 2 & 8, while at weeks 12, they did show alveolar bone formation. 7. At week 12, experimental group 2 showed numerous cells which appeared to be periodontal ligament cells, with functionally arranged connective tissue fibers between the roots and alveolar bone.

• Asian-Australasian Journal of Animal Sciences
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• 제29권4호
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• pp.539-548
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• 2016

The objective of the present study was to investigate the influence of palm kernel meal (PKM) inclusion and exogenous enzyme supplementation on growth performance, nitrogen-corrected apparent metabolizable energy (AMEn), coefficient of apparent ileal digestibility (CAID) and total tract retention of nutrients in young broilers fed corn-based diets. Four inclusion levels of PKM (no PKM [PKM0], 8% [PKM8], 16% [PKM16], and 24% [PKM24]) and two enzyme additions were evaluated in a $4{\times}2$ factorial arrangement of treatments. A total of 384, one-d-old male broilers (Ross 308) were individually weighed and allocated to 48 cages (eight broilers/cage), and cages were randomly assigned to eight dietary treatments. Results indicated that the inclusion of 8% and 16% PKM increased (p<0.05) the weight gain compared to the PKM0 diet. Birds fed the PKM8 diets had the highest (p<0.05) feed intake. Weight gain and feed intake were severely reduced (p<0.05) by feeding the PKM24 diet. Enzyme supplementation increased weight gain (p<0.05), independent of PKM inclusion level. In PKM0 and PKM8 diets, enzyme addition significantly (p<0.05) lowered feed conversion ratio (FCR); whereas enzyme addition had no effect on FCR of birds fed PKM16 and PKM24 diets. In PKM0 and PKM16 diets, enzyme addition significantly (p<0.05) increased CAID of nitrogen and energy but had no effect in the PKM8 and PKM24 diets. Inclusion of PKM into the basal diet, irrespective of inclusion level, enhanced (p<0.05) starch and fat digestibility. Inclusion of PKM at 16% and 24% resulted in similar CAID of neutral detergent fiber (NDF) but higher (p<0.05) than that of the PKM0 and PKM8 diets. Enzyme addition, regardless of the level of PKM inclusion, significantly (p<0.05) increased CAID of NDF. There was a significant (p<0.05) decrease in AMEn with PKM inclusion of 24%. The present data suggest that inclusion of PKM in broiler diets could be optimized if PKM-containing diets are formulated based on digestible amino acid contents and supplemented with exogenous enzymes. If amino acid digestibility and AME of PKM considered in the formulation, it can be included in broiler diets up to 16% with no deleterious effects on growth performance.

• 한국습지학회지
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• 제19권1호
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• pp.90-102
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• 2017

본 연구에서는 과거 2년에 걸쳐 강우유출수 처리목적의 인공습지로부터 수집된 수질자료를 바탕으로 지금까지 국외에서 개발된 다양한 형태의 영양소 모델의 적용성을 평가하였다. 검토결과 질소제거에 가장 큰 영향을 미치는 요인은 수온과 수리학적 부하율(HLR) 이었는데 습지의 수표면적이 습지의 용량에 비해 더 중요하게 작용했음을 시사해주고 있다. 질소제거는 미국 WEF(water environment federation)가 개발한 준경험적인 형태의 모델이 적합한 것으로 밝혀졌다. 한편 인 제거 모델은 수온과 제거속도상수의 관계를 Power Model로 보고 모의했을 때 실측치와 가장 가깝게 계산치를 산출할 수 있었으며 실측치의 증가와 감소경향을 비교적 정확하게 모의할 수 있었다($R^2=0.79$). 그러나 모든 시도에서 수온이 특정온도 이하로 감소했을 때 가장 심한 오차를 발생하여 취약한 예측성능을 보였다. 한편 인공습지에서 질소제거특성을 검토한 결과 질산화에 비하여 탈질이 저조한 것으로 나타났는데 그 이유는 탄소원의 부족과 높은 용존산소 농도로 인하여 Anoxic 조건의 미형성 때문으로 분석되었다. 질소제거 향상방안으로 용존산소의 제어와 탄소원의 확보차원에서 햇빛에 노출된 개방수역과 햇빛에 노출된 식생구역, 그리고 습지의 기능적 측면을 고려한 구성요소의 배분 및 부유 수생식물 및 무기성 여재를 이용하는 방안을 논의하였다.