• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.362-369
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• 1996

Seven different drying methods were tested in peony roots of Euisung cultivar, harvested in February, in three year's old plant. The roots were selected in length and diameter and half of the samples were removed cork-layers to compare the effects of cork-layer in processing of drying. The 3$0^{\circ}C$ heat drying without cork-layer reduced in days to drying by five days compared to those of the with cork-layers at the same temperature. The 5$0^{\circ}C$ heat drying after cork-layers removed was the most effective in days to drying. In quality of skin color of the 5$0^{\circ}C$ heating was worse to compare with the lower drying temperature. In the drying at room temperature and the heat drying at lower temperature, the paeoniflorin content in drying after cork-layers removed were higher than that of the drying with cork-layers. However, in the boiling water treatment, the paeoniflorin contents in drying after cork-layers removed were lower than those of with cork-layers. In heat drying, paeoniflorin content showed a decreasing tendency to increase of drying temperature. Total sugars in the peony roots showed a decreasing tendency according to the drying temperature increasing, but starch concentration showed a increasing tendency at the same condition. Concentrations of crude protein, crude fiber and crude ash were showed no differences in various drying methods and the materials with or without cork-layers. Relationships between the paeoniflorin and total sugars, and the paeoniflorin and starch were different significantly by the materials of cork-layers removed or not. The 30~4$0^{\circ}C$ heat drying without cork-layers was the most advisable condition for drying in paeoniflorin concentration, days to drying and skin color after drying.

• Archives of Plastic Surgery
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• v.40 no.5
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• pp.496-504
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• 2013

Background Amniotic-fluid-derived stem cells and amniocytes have recently been determined to have wound healing effects, but their mechanism is not yet clearly understood. In this study, the effects of amniotic fluid stem cells and amniocytes on wound healing were investigated through animal experiments. Methods On the back of Sprague-Dawley rats, four circular full-thickness skin wounds 2 cm in diameter were created. The wounds were classified into the following four types: a control group using Tegaderm disc wound dressings and experimental groups using collagen discs, amniotic fluid stem cell discs, and amniocyte discs. The wounds were assessed through macroscopic histological examination and immunohistochemistry over a period of time. Results The amniotic fluid stem cell and amniocyte groups showed higher wound healing rates compared with the control group; histologically, the inflammatory cell invasion disappeared more quickly in these groups, and there was more significant angiogenesis. In particular, these groups had significant promotion of epithelial cell reproduction, collagen fiber formation, and angiogenesis during the initial 10 days of the wound healing process. The potency of transforming growth factor-${\beta}$ and fibronectin in the experimental group was much greater than that in the control group in the early stage of the wound healing process. In later stages, however, no significant difference was observed. Conclusions The amniotic fluid stem cells and amniocytes were confirmed to have accelerated the inflammatory stage to contribute to an enhanced cure rate and shortened wound healing period. Therefore, they hold promise as wound treatment agents.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.132-141
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• 1994

In order to study the effects of silage additive on the quality and in preventing aerobic deterioration, reed canarygrass silages were made with various levels of formic acid, caproic acid and a mixture of the two acids. Preparation of silages cutted into about 1.5 cm length with the forage cutter and packed in vinyl bags(50 kg of capacity, 0.1 mrn of thickness), and it also divided nine types of experimental treatments. Aerobic deterioration in the silages were investigated for 7 days of aerobic exposure periods, and the plastic vessel of diameter 25 cm, a height 30 cm used as a tool. The results obtained from this experiment are summarized as fellow. 1. Crude protein and NFE contents at the time if opening were increased in the silage with mixture of formic and caproic acids(P<0.01). Crude fiber and ADF contents during the 7 days of aerobic exposure periods were the decreased in the sslages with mixture of two acods treatment(P<0.01). 2. Temperature of the silage treated with fromic acid alone during the 2 days of aerobic exposure period reached$34.4^{\circ}C$, while that of the silage with caproic acid and mixture formic and caproic acids were not rapidly increased resulting by reduced aerobic deteriorarion. 3. The pH of silages treated with formic acid alone during 7 days of aerobic exposure was not effectively changed, while solages treated with caproic acid were effectively stabilized. 4. The ratio of amminia nitrogen to total nitrogen was significantly decreased in silages with formic acid>caproic acid>mixture of both in the order(P<0.01). 5. Microbiological population of the silage were positively changed during the 7 days of aerobic exposure period. Solages with formic acid were deteriorated at ensiling observed, while applicarion of 1.2% caproic acid was partially reduced deteriorarion by precenting of the growth moulds.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.15-22
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• 2002

This study was conducted from April 13 to October 8, 2001 in Jeju to determine the influence of cutting height (2, 4, 6, 8 and 10 cm from the soil surface) on growth, forage yield and chemical composition of kenaf (Hibiscus cannabinus L.). As cutting height was increased from 2 to 10 cm, plant height averaged across two cuttings increased from 157.7 to 184.7 cm. This pattern held fir the number of leaves and branches per plant, stem diameter and plant weight per plant. As cutting height increased from 2 to 10 cm, fresh forage, dry matter, crude protein and TDN yields increased from 85.5 to 113.7MT/ha, from 11.97 to 15.63 MT/ha, from 1.63 to 2.72, and from 4.95 to 7.54 MT/ha, respectively. As cutting height was increased from 2 cm to 10 cm, crude protein, ether extract, nitrogen free extract, and TDN contents increased from 14.2 to 17.6%, 2.9 to 3.9%, 24.2 to 25.8% and 43.1 to 48.5%, respectively, while crude fiber and crude ash contents decreased from 35.5 to 30.4 % and 9.9 to 8.1 %, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.9-14
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• 2002

Evegradies 71' kenaf (Hibiscus cannabinus L.) was grown at 160,000 plants per ha in a volcanic ash soil in Jeju island to determine the optimum frequency of split N application fer forage production. Nitrogen fertilizer was applied with 240 kg/ha, and frequencies of the split N applications were 1, 2, 3, 4 and 5 times. Plant height averaged across two harvests increased from 187 to 201cm as N was split-applied from one to three applications and then decreased to 201cm in five applications. This pattern held for the number of branches and leaves per plant stem diameter and weight of plant per plant. Fresh forage yield increased from 91.8 to 114.2 MT/ha. dry matter yield from 12.70 to 16.6 MT/ha, crude protein yield from 1.75 to 2.48MT/ha, and total digestible nutrients (TDN) yield from 5.39 to 7.63 MT/ha as N was split-applied from one to four applications, and then decreased to 106.6, 15.0, 2.32, and 7.22 MT/ha in five applications, respectively As N was split-applied from one to five applications, crude protein content increased from 13.8 to 15.4%. ether extract content from 4.1 to 5.9%, and TDN content from 42.4 to 48.1%. but crude fiber decreased from 38.2 to 37.1% and crude ash content from 11.5 to 8.6%. Nitrogen free extract content was about 19.0% regardless of cutting height.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.225-232
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• 2001

In order to identify the optimum seeding rate of Jeju barnyard grass (Echinochloa crusgalli var. fiurnentacea(Roxb) Wight), 15, 20, 25, 30, 35 and 40 kilograms per a hectare were planted in April 16, 2000, respectively. The growth characterization, the yield and chemical composition were then investigated in June 28(1st cutting) and August 30(2nd cutting), 2000. According to increasing the seeding rate, plant height(155.5 to 162 cm), the yield of fresh forage(61 to 73 MT/ha), dry matter forage(11.9 to 16.9 MT/ha), crude protein(0.9 to 1.6 MT/ha), and TDN(6.1 to 9.7 MT/ha) were gradually increased until the seeding furrow, 35 kg/ha. In 40 kg/ha of seeding furrow, however, those results were decreased than that of the former seeding furrow. The contents of crude protein(7.4 to 10.4%), ether extract(3.3 to 5.2%), nitrogen free extract(46.4 to 47.8%), and TDN(52.1 to 60.4%) were also similar to those of the plant heights and the each yield excepted the seeding furrow, 40 kg/ha. Namely, the each results about the contents increased in 40kg/ha of seeding furrow. According to increasing the seeding rate, the stem diameter, number of leaves, number of withering leaves, and fresh weight were gradually decreased. The contents of crude fiber(30.3 to 27.5%) and crude ash(12.7 to 9.0%) were also decreased These results showed that the optimum seeding rate for the best of the feed production was the seeding furrow, 35 kg/ha.

• Food Science and Preservation
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• v.9 no.2
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• pp.228-233
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• 2002

In order to exploit as a new functional decocted beverage, chemical components, antioxidative and antimicrobial activities young stems of mulberry(Morus alba) were surveyed. The proximate composition was composed of crude fiber 51.12%, ash 13.46%, total sugar 10.38%, crude fat 9.10% and crude protein 5.01%. The P(295.9 mg%) was the highest mineral found in young stem of mulberry and Ca 289.6 mg%, K 209.6 mg%, Na 58.3 mg%, Mg 45.0 mg% and Fe 4.6 mg% in that order. Free sugars was composed of glucose 1.08%, galactose 022%, sucrose 0.20% and fructose 0.16%. Eight fatty acids in stem of mulberry were identified and the major fatty acids were linoleic acid(46.10%), palmitic acid(27.84%) and linolenic acid(10.85%). Among the 17 amino acids detected, total amino acid was 2,450.5 mg% and proline(313.7 mg%) was the most predominant. Methanol extract and ethyl acetate fraction showed stronger activity of the hydrogen donating activities, each of 77.24% and 80.08%, respectively. The methanol extract from young stem of mulberry showed the strongest antimicrobial activities to Bacillus subtitis and Bacitus cereus. Chloroform and ethyl acetate fractions from methanol extract of young stem showed a inhibition zone of 9.0∼19.0 ㎜ in diameter against pathogen bacteria.

• Journal of Periodontal and Implant Science
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• v.34 no.3
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• pp.543-549
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• 2004

Chitosan has been widely researched as bone substitution materials and membranes in orthopedic/periodontal applications. Chitosan nanofiber membrane was fabricated by chitosan nanofiber using electrospinning technique. The structure of the membrane is nonwoven, three-dimensional, porous, and nanoscale fiber-based matrix. The aim of this study was to evaluate the biocompatibility of chitosan nanofiber membrane and to evaluate its capacity of bone regeneration in rabbit calvarial defect. Ten mm diameter round cranial defects were made and covered by 2 kinds of membranes (Gore-Tex membrane, chitosan nanofiber membrane) in rabbits. Animals were sacrificed at 4 weeks after surgery. Decalcified specimens were prepared and observed by microscope. Chitosan nanofiber membrane maintained its shape and space at 4 weeks. No inflammatory cells were seen on the surface of the membrane. In calvarial defects, new bone bridges were formed at all defect areas and fused to original old bone. No distortion and resorption was observed in the grafted chitosan nanofiber membrane. However bone bridge formation and new bone formation at the center of the defect could not be seen in Gore-Tex membranes. It is concluded that the novel membrane made of chitosan nanofiber by electrospinning technique may be used as a possible tool for guided bone regeneration.

• Korean Journal of Optics and Photonics
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• v.10 no.3
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• pp.195-200
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• 1999

We made sharp optical fiber tips with less than 100 nm diameter by using the heating and pulling method with a good repetition and fabricated the photon scanning tunneling microscope (PSTM) using constant intensity mode. The 3-dimensional PZT (Piezoelctric transducer) scanner made of a long PZT tube is consisted of three divided parts, that is, a pair of $\pm$ x and a pair of $\pm$y scanning parts and a z scanning part for the fine approach and scanning. The scanning dimension is 1.43 $\mu\textrm{m}$$\times$1.76 $\mu\textrm{m}$. The height of a optical tip to maintain a constant height within $1/{\lambda}_0$ (${\lambda}_0$ is the incident wavelength) from surface of a specimen to a optical tip is controlled automatically by using the electric feedback circuit. The 3-dimensional shape of standing evanescent waves generated on the surface of a dove prism was measured successfully by using the constant intensity mode PSTM.

• Journal of Life Science
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• v.27 no.12
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• pp.1479-1485
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• 2017

Muscle atrophy due to aging, starvation, and various chronic diseases leads to a decrease in muscle fiber area and density due to reduced muscle protein synthesis and increased protein breakdown. This study investigated the effect of dexamethasone and hydrogen peroxide on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes. C2C12 myoblasts were differentiated into myotubes in differentiation medium. During myoblast differentiation, muscle-specific transcription factors, such as myogenin, and MyoD expression increased. Differentiated C2C12 myotubes exposed to noncytotoxic levels of dexamethasone and hydrogen peroxide showed a decrease in myotube diameter, which was associated with up-regulation of muscle-specific ubiquitin ligases, such as muscle atrophy F-box (MAFbx)/atrogin-1 and muscle RING finger-1 (MuRF1), and down-regulation of myogenin and MyoD. These results demonstrated that dexamethasone and hydrogen peroxide induced atrophy through regulation of muscle-specific ubiquitin ligases and muscle-specific transcription factors in C2C12 myotubes. In this study, we confirmed the process of differentiation of C2C12 myoblasts into myotubes in in vitro experiments in the presence of atrophy. This muscle atrophy model of C2C12 cells induced by dexamethasone or hydrogen peroxide seems suited to studies of the mechanism of muscle atrophy suppression and to exploit the experiment for excavating new muscle atrophy.