• Weed & Turfgrass Science
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• v.3 no.4
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• pp.370-377
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• 2014

Research was initiated to evaluate cool-season grass (CSG) overseeding effects on coverage and species transition in Korean lawngrass (Zoysia japonica Steud.) and to determine CSGs and their seeding rate for a practical overseeding. Treatments were comprised of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), tall fescue (TF, Festuca arundinacea Schreb.) and their mixtures. Significant differences were observed in turfgrass coverage and species transition. Since overseeding, the coverage was highest in PR, followed by TF and lowest in KB. The highest coverage in polystand was associated with Mixture IV (TF 50 + PR 50) at a seeding rate of $150gm^{-2}$ which had highest PR and TF, but without KB. Regarding turfgrass transition, zoysiagrass recovery continued to come back up to 80% from late March. But it reduced after early October. Zoysiagrass in monostand was greatest in KB and lowest in TF, but variable in polystand. For the fast and great coverage in winter, it would be the best to apply with PR at $100gm^{-2}$ and equal combination of PR and TF by 1/2 in the mixture at $150gm^{-2}$. For the smooth spring transition to zoysiagrass, however, KB application at $50gm^{-2}$ is recommended.

• Korean Journal of Environment and Ecology
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• v.22 no.5
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• pp.481-504
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• 2008

This study was carried out to investigate the flora of Mt. Biseul. The vascular plants collected 10 times(from May 2006 to October 2007) were identified as 468 taxa in total, including 89 families, 302 genera, 397 species, 1 subspecies, 59 varieties and 11 forms. Korean endemic plants of this area were identified as 17 taxa including Thalictrum uchiyamai, Coreanomecon hylomeconoides, Lonicera subsessilis, etc.; the Ministry of Environment-designated plants, which should be protected by the wildlife protection law, were identified as one taxon including Aconitum austrokoreense; the Office of Forestry-designated rare & endangered plants were identified as 6 taxa including Viola albida, Lloydia triflora, Streptopus ovalis, etc.; the naturalized plants were identified as 34 taxa, and their naturalization ratio and urban index were found to be 7.3%, and 12.2% respectively. This study newly identified about 160 taxa of the plants, among which 17 taxa were newly added to the list of naturalized plants, such as Phytolacca americana, Dicentra spectabilis, Lepidium apetalum, Medicago lupulina, Euphorbia supina, Ambrosia artemisiifolia, Cosmos bipinnatus, Crassocephalum crepidioides, Galinsoga ciliata, Rudbeckia bicolor, Sonchus oleraceus, Tagetes minuta, Taraxacum officinale, Chloris virgata, Festuca arundinacea, Lolium perenne, Tradescantia reflexa, etc. There existed many kinds of plants resources having conservational value like Aconitum austrokoreense etc. in this area, but there remains a fear of nature destruction's acceleration due to indiscriminate human development and access of a lot of visitors, so it is judged that there should be a management plan, such as a limit on the number of visitors or rest-year-system for restoration of nature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.237-242
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• 2004

A system for the production of transgenic plants has been developed for tall fescue (Festuca arundinacea Schreb.) via Agrobacterium-mediated transformation of mature seed-derived embryogenic callus. Seed-derived calli were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase (HPT), neomycin phosphotransferase II (NPTII) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. Inclusion of $200\mu\textrm{M}$ acetosyringone (AS) in inoculation and co-culture media lead to a increase in stable transformation efficiency. Transformation efficiency was increased when embryogenic calli were co-cultured for 5 days on the co-culture medium. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agyobacterium in the presence of 0.1% Tween20 and $200\mu\textrm{M}$ AS. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and Southern blot analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of tall fescue.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.2
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• pp.119-124
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• 2015

In this study, we sought to evaluate the growth characteristics and seed productivities of domestic tall fescue that were cultivated with differing combinations of drill widths and nitrogen-application rates in early spring; the examination was conducted at the National Institute of Animal Science, RDA, from 2013 to 2014. The main plots were three widths of 15 cm, 30 cm, and 45 cm, and the subplots were subject to nitrogen-application rates in early spring of 45 kg/ha, 90 kg/ha, 135 kg/ha, and 180 kg/ha. The growth and development characteristics did not vary by treatment in terms of drill width and early-spring nitrogen application. Tall fescue Purumi showed a stable seed productivity of two tons or greater when the drill width was set at 15 cm and 90 kg/ha of nitrogen was applied in early spring. The narrower the drill width, the greater the numbers of both the ears and seeds, while the length of the ear was longer when the drill width was wider. Regarding the influence of the drill width and early-spring nitrogen application on the productivity of seed-producing straw, the dry matter productivity was higher when the drill width was narrower; however, no particular trend was observed with respect to different amounts of fertilizer. The average production amount of the first round of straw after seed gathering was 6,920 kg/ha. The second round produced an average 8,134 kg/ha of dry matter.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.1
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• pp.61-71
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• 2016

The purpose of the research is to identify the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria as leguminous woody plants for the cut-slope revegetation. To investigate the coverage ratio and appearance frequency, we divided Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria into five treatment groups with various quantities of $0.0g/m^2$, $1.0g/m^2$, $2.4g/m^2$, $3.8g/m^2$, and $5.2g/m^2$. For each treatment group, we mixed the identical quantities of herbaceous flowers (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena and Pennisetum alopecuroides) seeds and cool-season turfgrasses (Festuca arundinacea and Poa pratensis) seeds. In this result, as the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria increased in the spray, the coverage ratio for leguminous woody plants appeared to increase whereas the coverage ratio decreased in herbaceous flowers and cool-season turfgrasses. However, when the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria exceeded $3.8g/m^2$ in the spray, the coverage ratio of leguminous woody plants rather decreased compared to four treatment groups less than $3.8g/m^2$ seeding ratio. Based on the longitudinal data of coverage ratio in five treatment groups, we observed the gradual process of a short-term succession in which the dominant species shifted in the following order: First, cool-season turfgrasses; Second, herbaceous flowers; Third, leguminous woody plants. Comparing the appearance frequency of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria, Lespedeza cyrtobotrya appeared more frequent in 2014 whereas Indigofera pseudo-tinctoria appeared to be relatively more frequent in 2015. As a result, Indigofera pseudo-tinctoria was discovered to be a dominant species among woody plants. In this study, we observed that the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria was $2.4g/m^2{\sim}3.8g/m^2$. Moreover, the coverage ratio of 29.1%~35.4% and appearance frequency of 4.6plants~5.8plants were found in the condition of optimal seeding quantity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.2
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• pp.77-82
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• 2006

An efficient transformation system for the production of transgenic plants has been developed for tall fescue (Festuca arundinacea Schreb.) via Agrobacterium-mediated transformation of seed-derived callus. From the point of morphogenetic capacity, three types of callus were selected. High frequency of plant regeneration was obtained by selection of type II callus, and the plant regeneration frequency was 52.6% when embryogenic callus were cultured on the regeneration medium. Supplementation of the media with 10 mg/L $AgNO_3$ and 40 mg/L cysteine enhanced frequencies of plant regeneration up to 65.3%. The highest transformation efficiency was also obtained when type II callus were inoculated with Agrobacterium. Southern blot analysis of PCR products of transgenic plants demonstrated that transgenes were successfully integrated into the genome of tall fescue. Efficient regeneration system and transformation established in this study will be useful for molecular breeding of tall fescue through genetic transformation.

• Asian Journal of Turfgrass Science
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• v.16 no.2
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• pp.65-73
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• 2002

Studies were conducted to determine the effect of undersoil heating on growth and quality of turfgrasses including Kentucky bluegrass (Poa pratensis L.‘Nuglade’), perennial ryegrass (Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb.‘Pixie’), and Korean lawngrass (Zoysia japonica Steud.) in simulated athletic field during winter season in Korea. Mineral contents in clippings of turfgrasses grown at different soil mixtures and temperatures were also analyzed. Undersoil heating (approximately 20$\pm$2$^{\circ}C$) was effective in protecting turfgrasses except Korean lawngrass from freezing injury and discoloration of shoots due to extremely cold temperatures during midwinter. Among turfgrasses grown at undersoil heating zone, tall fescue and perennial ryegrass showed the highest clipping weights and chlorophyll contents, respectively. However, anthocyanin contents of shoots were higher in Kentucky bluegrass. There was little or no difference in clipping weights, chlorophyll contents, anthocyanin contents and greenness of shoots between turfgrasses grown at two soil mixtures composed of 80% sand＋10% peat moss＋10% soil (v/v/v) and 80% sand＋20% pea moss (v/v). Contents of mineral K, Ca and Mg in clippings of cool-season turfgrasses were comparatively higher in a soil mixture composed of 80% sand＋10% peat moss＋10% soil, but little difference in contents of N and P was observed between two soil treatments. Results indicated that undersoil heating can improve quality of turf surface by thawing soil, melting snow, and maintaining shoot growth and greenness of turfgrasses in sports field during winter season.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.23-34
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• 2009

Research was initiated to investigate early establishment characteristics and germination pattern of tall fescue(TF, Festuca arundinacea Shreb.). Four varieties of TF were evaluated in the study. Experiment was conducted under a room temperature condition of $5^{\circ}C$ to $25^{\circ}C$(natural conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination patterns, days to the first germination, days to 50% germination, days to 75% germination, and germination percentage with different environments and varieties. Germination percentage was variable with varieties at the end of study. It was 85.25 to 97.00% under natural conditions. There were considerable variations in early germination characteristics and patterns among the entries according to different conditions. The first germination was initiated between 7 and 9 DAS(days after seeding) under natural conditions, being 2 to 3 days later as compared with ISTA conditions. It was 11 to 12 DAS in days to the 50% germination, which was 3 to 4 days after the first germination. Days to the 75% germination were 12.38 to 14.29 DAS, being 1.55 to 4.77 days slower as compared with ISTA conditions. Olympic Gold was regarded as the fastest variety and Tar Heel II was slowest. Data from this study demonstrated that information on differences in germination characteristics and patterns among varieties would be usefully applied for TF establishment such as golf course construction.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.227-244
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• 2013

The vascular plants in the trail from Jangansan to Palgongsan were listed 389 taxa (8.0% of all 4,881 taxa of Korean vascular plants); 84 families, 226 genera, 325 species, 1 subspecies, 55 varieties and 8 forms. Furthermore, hemicryptophytes (H) were 110 taxa (28.3%), geophytes (G) were 77 taxa (19.8%), megaphanerophytes (MM) were 48 taxa (12.3%), nanophanerophytes (N) were 47 taxa (12.1%) showed high proportional ratio in life form. So, resource plants of 237 taxa of edible (60.9%), 278 taxa of medicinal (71.5%), 229 taxa of ornamental (58.9%) and 178 taxa of the others (45.8%). Based on the list of rare plants, 7 taxa (1.8% of all 389 taxa of flora and 1.2% of all 571 taxa of rare plants); Paeonia japonica (VU), Viola albida, Viola diamantica, Lilium distichum, Disporum ovale, Tricyrtis dilatata, Iris ensata var. spontanea (LC), etc. Based on the list of endemic plants, 7 taxa (1.8% of all 389 taxa of flora and 2.1% of all 328 taxa of endemic plants); Salix koriyanagi, Aconitum pseudolaeve var. erectum, Thalictrum actaefolium var. brevistylum, Philadelphus schrenckii, Asperula lasiantha, Weigela subsessilis, Carex okamotoi, etc. Based on the list of specific plants, 46 taxa (11.8% of all 389 taxa of flora and 4.3% of all 1,071 taxa of specific plants); 2 taxa (Wisteria floribunda for. floribunda, Cardamine yezoensis, etc.) in class IV, 6 taxa (Acer palmatum, Ulmus davidiana, etc.) in class III, 14 taxa (Ligularia fischeri, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Ilex macropoda, Fraxinus mandshurica, etc.) in class I. Based on the list of naturalized plants, 6 families, 16 genera, 18 taxa (Phytolacca americana, Robinia pseudoacacia, Oenothera odorata, Erechtites hieracifolia, Festuca arundinacea, etc.) and invasive alien plants were 3 taxa; Rumex acetocella, Ambrosia artemisiifolia, Aster pilosus. Naturalization rate (NR) was 4.6% of all 389 taxa of vascular plants and urbanization index (UI) was 5.6% of all 321 taxa of naturalized plants.

• Journal of Wetlands Research
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• v.14 no.4
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• pp.699-713
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• 2012

Palustrine wetland is often used for development work and green area creation. However, the ecological characteristic of vegetation is not frequently considered in planning. Therefore, this study was conducted to investigate vegetation characteristic according to land-use of palustrine wetland and to provide basic materials in assessment of palustrine wetland and restoration and creation of wetland. Survey sites were caterogized into paddy, field, and mountain. Each category had two sites, so total 6 survey sites were investigated for vegetation. As a result, with a range of 61~92 species, total 216 taxa were confirmed, consisting of 71 families, 168 generic, 187 species, 2 variety 1 forma 1 subforma. Mountain received a high score in the evaluation score range of floristic regional indicator plants, and it was found that there were 10 families and 28 species of naturalized plant. Mountain showed low appearance, and fiddy and field showed relatively high appearance. Naturalized plants with slightly high vegetation cover ratio are Erigeron annuus, Amorpha fruticosa, Dactylis glomerata, Festuca arundinacea, Eragrostis curvula, Trifolium repens, Erigeron Canadensis, Ambrosia trifida, Oenothera odorata, Bidens frondosa, etc. Such naturalized plants should be removed to prevent terrestrialization of wetland and its harmful damages. Long-term monitoring and proper management of naturalized plants are expected to improve palustrine wetland into high quality biotope of rural area in the future.