• 한국식품영양학회지
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• 제25권3호
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• pp.499-504
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• 2012

흑진주벼, 추청벼, 신동진벼의 현미상태에서 ethanol을 이용하여 ${\gamma}$-oryzanol 추출물을 제조한 다음, 이들의 조성 및 함량을 HPLC로 분석하고 생리활성을 측정하여 비교한 결과는 다음과 같다. 인용문헌을 바탕으로 총 10종의 개별 성분을 분리 및 확인하였으며, 이 중 cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, campesteryl ferulate, sitosteryl ferulate이 주요 성분으로 확인되었다. ${\gamma}$-Oryzanol 총함량은 흑진주벼, 추청벼, 신동진벼의 순으로 나타났으며, 이들 ${\gamma}$-oryzanol 추출물에 대한 DPPH 라디칼 소거율, 콜레스테롤 저하 효과, 항균효과 역시 흑진주벼, 추청벼, 신동진벼 순으로 나타나 ${\gamma}$-oryzanol이 이들 활성에 있어서 중요한 역할을 하는 것으로 판단되었다. 특히 항산화 효과에 있어 합성항산화제와 비교하였을 때 전반적으로 매우 높게 나타나, 이러한 결과들을 바탕으로 현미에 함유된 ${\gamma}$-oryzanol이 식품, 제약, 의료 분야 등 다방면에서 활용될 수 있을 것으로 기대한다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.202-202
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• 2005

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.187.1-187
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• 2003

No Abstract, See Full Text

• 대한화장품학회지
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• 제28권3호
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• pp.91-108
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• 2002

미백제는 기미와 태양광선노출에 의한 색소침착반과 같은 색소불균형이 주로 나타나는 동양 여성들에 특히 관심이 많다. 이러한 색소침착의 불균형은 UV에 의해 더 심해지기 때문에 미백제는 멜라닌합성을 억제시키거나 UV에 의해 활성화된 signal을 억제함으로써 효과가 나타난다. Eumelanin은 UV에 의해 유도된 DNA 손상을 보호하므로 UV에 의해 유도된 DNA손상을 감소시키는 물질이 이상적인 미백제가 된다. 새롭게 합성된 항산화제인 $\alpha$-tocophenyl ferulate의 효과는 UV에 의해 손상된 DNA damage에 의한 보호 효과와 멜라닌 합성을 억제시킴으로서 나타난다. Chemical peeling과 복합적으로 색소 반점을 치료하는 lightning agent의 효과에 대한 결과를 보고하였으며 색소 반점의 개선을 정량적으로 평가하는 새롭게 개발된 facial image analyzer를 소개할 것이다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 추계학술발표회 요지
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• pp.120-121
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• 2005

• 대한화학회지
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• 제13권4호
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• pp.325-332
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• 1969

An attempt has been made to investigate the nature of the characteristic darkening reaction occurring in the rice bran oil during the storage. Several pigments were separated by using column and thin layer chromatography of the pigments was made in the light of the knowledge of absorption spectral behavior. The presence of trace amount of iron and phytosteryl esters of ferulic acid (3-methoxy-4-hydroxy-cinnamic acid) was found to be responsible for the development of the characteristic color in rice bran oil. The model reactions with the ferulate, iron and fatty acids could be suggested the mechanism.

• 생약학회지
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• 제38권4호
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• pp.403-408
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• 2007

xBrassicoraphanus is an intergenic breed crossed between Brassica campetris L. ssp. pekinensis and Raphanus sativus L. that have been daily consumed. xBrassicoraphanus was known to have good tastes and biological activities. Nevertheless, its constituetnts were not elucidated yet. Thus, in the present study, to indirectly evaluate the biological activity of xBrassicoraphanus, 12 compounds were isolated from leaves and roots of xBrassicoraphanus. On the basis of spectroscopic evidences, the structures of these compounds isolated from leaves of xBrassicoraphanus. were identified as ${\beta}-sitosterol$, indole-3-acetonitrile, ferulic acid, methyl ferulate, linolenic acid methyl ester, linolenic acid and coniferyl alcohol, while the chemical structures of compounds isolated from the roots of were xBrassicoraphanus were characterized as ${\beta}-sitosterol$, indole-3-acetonitrile, ferulic acid, methyl ferulate, linolenic acid methyl ester, 1-methoxyindole-3-acetonitrile, goitrin, 4-hydroxycinnamyl alcohol, coniferyl alcohol, palmitic acid and daucosterol. These can be classified as three steroids, two indole cyanides, two cinnamic acid derivatives, one cinnamyl alcohol derivative, three fatty acid derivatives one isothiocyanate. These results suggest that the compounds isolated from xBrassicoraphanus were almost identical with known components of Brassica campetris L. ssp pekinensis or Raphanus sativus L. However, it is necessary to investigate more about the difference of amounts of constituents according to harvest time and variant species amounts.

• Archives of Pharmacal Research
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• 제26권7호
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• pp.535-539
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• 2003

The EtOAc soluble fraction from the stem of Sorghum bicolor showed a strong free radical scavenging activity. Five major compounds were isolated from this fraction. They were identified by spectral data as methyl ferulate (1), methyl p-hydroxycinnamate (2), p-hydroxybenzaldehyde (3), tricin (4), and quercetin 3,4 -dimethyl ether (5). Among these compounds, 1 exhibited a strong, free radical scavenging activity on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) with an $IC_50$ value of 0.7 $\mu$M. We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. All five compounds showed anti-lipid peroxidation activity ($IC_50$ values of 0.5, 0.4, 0.3 and 0.3 $\mu$ M, respectively).

• 생약학회지
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• 제38권3호통권150호
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• pp.222-226
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• 2007

Seven compounds, scopoletin (1), p-hydroxyphenethyl trans-ferulate (2), $1-({\alpha}-L-rhamnosyl(1\;{\rightarrow}\;6)-{\beta}-D-glucopyranosyloxy)-3,4,5-trimethoxybenzene$ (3), benzyl ${\alpha}-L-rhamnopyranosyl(1\;{\rightarrow}\;6)-{\beta}-D-glucopyranoside$ (4), suberic acid (5), sebacic acid (6) and scopolin (7) were isolated from the methanol extract of the roots of Althaea rosea Canival. Compounds $1\;{\sim}\;7$ were first isolated from this plant. The isolated compounds were tested for their cytotoxicity against human cancer cell lines using a SRB method in vitro.

• Journal of Photoscience
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• 제9권2호
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• pp.201-204
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• 2002

Epidermal cells produce a panel of antioxidants as well as cytokines after UVB irradiation, which counteract reactive oxygen species, however, how these antioxidants might regulate melanogenesis is unclear. An important constituent of the cellular antioxidant buffering system which controls the redox state of proteins is thioredoxin (TRX), a 13-kD protein that catalyzes thiol-disulfide exchange reactions, regulates activation of transcription factors, and possesses several other biological functions similar to cytokines. TRX suppressed the UVB-induced production and secretion of $\alpha$-melanocyte stimulating hormone ($\alpha$-MSH) and of adrenocorticotropic hormone (ACTH), and also suppressed proopiomelanocortin (POMC) mRNA expression by normal human keratinocyte (KC)s. Further, L-cysteine, N-acetyl-cysteine, $\alpha$-tocopheryl ferulate showed suppressive effect on UVB-induced POMC mRNA expression. However, TRX released from UVB-irradiated KCs stimulated melanogenesis by up-regulating MSH receptor expression and its binding activity in melanocyte (MC)s. UVB-induced KC derived cytokines such as IL1, IL6, and ET1 upregulated MSH-receptor binding ability as well as MCl-R mRNA expression in cultured normal human MCs. MCl-R has a tendency to be upregulated by UVB-induced KC-derived cytokines as well as by direct UVB irradiation. These results suggest that antioxidants such as TRX suppresses UVB induction of POMC, but in the case of MCl-R, this gene can be mainly in the trend of upregulation by UVB-induced KC-derived factors including TRX.