The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Quantification of γ-Oryzanol Components and Comparison Its Biological Activity in Brown Rice

현미(추청벼, 흑진주벼, 신동진벼)에 함유된 γ-Oryzanol의 함량 분석 및 생리활성 효과 비교

  • Received : 2012.07.25
  • Accepted : 2012.08.08
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

We analyzed the contents of ${\gamma}$-oryzanol, which is contained in brown rice of the nation rice varieties Chucheong, Heukjinju and Sindongjin, by HPLC. Furthermore we also performed experiments on its biological activity, to prove the effectiveness of rice bran. The contents of ${\gamma}$-oryzanol contained in hulled rice showed 1,587 ppm for Heukjinju, followed by Chucheong(1,038 ppm), and by Sindongjin(472 ppm). In anti-oxidative activity, we performed an experiment, by measuring the radical scavenging activity of DPPH. Heukjinju showed the best effect, and Chucheong showed the worst effect. In cholesterol lowering activity, Heukjinju showed the best activity and Sindongjin showed the worst effect. In anti-bacterial activity as well, Heukjinju showed the best activity, and Sindongjin showed the worst effect. Through these experiments, we compared the contents of ${\gamma}$-oryzanol, which is contained in hulled rice(Chucheong, Heukjinju, Sindongjin). Also, we found the anti-oxidation effect, cholesterol lowering effect, and anti-bacterial activity of the ${\gamma}$-oryzanol extracts. Based on our research, we expect that ${\gamma}$-oryzanol can work as a new drug, or nutritional supplement.

흑진주벼, 추청벼, 신동진벼의 현미상태에서 ethanol을 이용하여 ${\gamma}$-oryzanol 추출물을 제조한 다음, 이들의 조성 및 함량을 HPLC로 분석하고 생리활성을 측정하여 비교한 결과는 다음과 같다. 인용문헌을 바탕으로 총 10종의 개별 성분을 분리 및 확인하였으며, 이 중 cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, campesteryl ferulate, sitosteryl ferulate이 주요 성분으로 확인되었다. ${\gamma}$-Oryzanol 총함량은 흑진주벼, 추청벼, 신동진벼의 순으로 나타났으며, 이들 ${\gamma}$-oryzanol 추출물에 대한 DPPH 라디칼 소거율, 콜레스테롤 저하 효과, 항균효과 역시 흑진주벼, 추청벼, 신동진벼 순으로 나타나 ${\gamma}$-oryzanol이 이들 활성에 있어서 중요한 역할을 하는 것으로 판단되었다. 특히 항산화 효과에 있어 합성항산화제와 비교하였을 때 전반적으로 매우 높게 나타나, 이러한 결과들을 바탕으로 현미에 함유된 ${\gamma}$-oryzanol이 식품, 제약, 의료 분야 등 다방면에서 활용될 수 있을 것으로 기대한다.

Keywords

References

  1. Akihisa T, Yasukawa K, Yamaura M, Ukiya M, Kimura Y, Shimizu N, Arai K. 2000. Triterpene alcohol and sterol ferulates from rice bran and their anti-inflammatory effects. J Agric Food Chem 48:2313-2319 https://doi.org/10.1021/jf000135o
  2. Blios MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  3. Fujita A, Masumoto K, Kawakami K, Mikami T, Nomura M. 2005. Anti-oxidation activity of various rice brans. J Oleo Sci 55:585-591
  4. Hu W, Wells JH, Shin, TS Godber IS. 1996. Comparison of isopropanol and hexane for extraction of vitamin E and oryzanols from stabilized rice bran. J Am Oil Chem Soc 73:1653-1656 https://doi.org/10.1007/BF02517967
  5. James GC, Sherman J. 1978. Chemotheraperapeutic Agent in Microbiology, a Laboratory Manual Chemical Agents of Control. pp.247-254 Prentice Hall, USA
  6. Juliano C, Cossu M, Alamanni MC, Piu L. 2005. Antioxidant activity of gamma-oryzanol: Mechanism of action and its effect on oxidative stability of pharmaceutical oils. Int J Pharm 299:146-154 https://doi.org/10.1016/j.ijpharm.2005.05.018
  7. Kim IH, Kim CJ, You JM, Lee KW, Kim CT, Chung SH, Tae BS. 2002. Effect of roasting temperature and time on the chemical composition of rice germ oil. J Am Oil Chem Soc 79:413-418 https://doi.org/10.1007/s11746-002-0498-2
  8. Kim JS. 2007. Antioxidant activity of $\gamma$-oryzanol and synthetic phenolic compounds in an oil/water(O/W) emulsion system. J Food Sci Nutr 12:173-176 https://doi.org/10.3746/jfn.2007.12.3.173
  9. Miller A, Engel KH. 2006. Content of $\gamma$-oryzanol and composition of steryl ferulates in brown rice(Oryza sativa L.) of European origin. J Agric Food Chem 54:8127-8133 https://doi.org/10.1021/jf061688n
  10. Nakayama S, Manabe A, Suzuki J, Sakamoto K, Inagaki T. 1987. Comparative effects of two forms of $\gamma$-oryzanol in different sterol compositions on hyperlipidemia induced by cholesterol diet in rats. Jpn J Pharmacol 44:135-143 https://doi.org/10.1254/jjp.44.135
  11. Sakamoto K, Tabata T, Shirasaki K, Inagaki T. 1987. Effects of $\gamma$-oryzanol and cycloartenol ferulic acid ester on cholesterol diet induced hyperlipidemia in rats. Jpn J Pharmacol 45:559-565 https://doi.org/10.1254/jjp.45.559
  12. Shin TS, Godber JS, Martin DE, Wells JH. 1997. Hydrolytic stability and changes in E vitamers and oryzanol of extruded rice bran during storage. J Food Sci 62:704-728 https://doi.org/10.1111/j.1365-2621.1997.tb15440.x
  13. Wilson TA, Nicolosi RJ, Woolfrey B, Kritchevsky D. 2007. Rice bran oil and oryzanol reduce plasma lipid and lipoprotein cholesterol concentrations and aortic cholesterol ester accumulation to a greater extent than ferulic acid in hypercholesterolemic hamsters. J Nutr Biochem 18:105-112 https://doi.org/10.1016/j.jnutbio.2006.03.006
  14. Xu Z, Godber JS. 1999. Purification and identification of components of $\gamma$-oryzanol in rice bran oil. J Agric Food Chem 47:2724-2728 https://doi.org/10.1021/jf981175j
  15. Xu Z, Godber JS. 2000. Comparison of supercritical fluid and solvent extraction methods in extracting $\gamma$-oryzanol from rice bran. J Am Oil Chem Soc 77:547-551 https://doi.org/10.1007/s11746-000-0087-4
  16. Yu S, Nehus ZT, Badger TM, Fang N. 2007. Quantification of vitamin E and $\gamma$-oryzanol components in rice germ and bran. J Agric Food Chem 55:7308-7313 https://doi.org/10.1021/jf071957p
  17. http://ko.wikipedia.org. 2011.04.20 '쌀' 검색

Cited by

  1. Effects of high hydrostatic pressure treatment on the enhancement of functional components of germinated rough rice (Oryza sativa L.) vol.166, 2015, https://doi.org/10.1016/j.foodchem.2014.05.150
  2. Treatment Effect of Green Manure Crops on Content of γ-Oryzanols from Korean Rice Variety, Unkwangbyeo vol.34, pp.2, 2015, https://doi.org/10.5338/KJEA.2015.34.2.20
  3. Changes in the Functional Components of Barley Produced from Different Cultivars and Germination Periods 2017, https://doi.org/10.1094/CCHEM-05-17-0114-R
  4. Cultivar Comparison on Tocopherols, Tocotrienols, and Antioxidant Compounds in Rice Bran vol.58, pp.4, 2013, https://doi.org/10.7740/kjcs.2013.58.4.367
  5. Influence of Applied Pressure on Bioactive Compounds of Germinated Rough Rice (Oryza sativa L.) vol.8, pp.10, 2015, https://doi.org/10.1007/s11947-015-1565-1