• Journal of Nutrition and Health
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• 제35권6호
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• pp.643-649
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• 2002

This study was done to investigate effects of ${\gamma}$-irradiated pork feeding on the formation of glutathione S-transferase placental form positive (GST-P$^{+}$) foci, lipid peroxidation, cytochrome P450 system and microsomal glucose 6-phosphatase activity in diethylnitrosamine (DEN)-initiated rat hepatocarcinogenesis. Weaning Sprague-Dawley male rats were fed the diet containing ${\gamma}$-irradiated ground pork at the dose of 0, 3, 10, 30 kGy as a 20％ of protein source for 8 weeks. One week after feeding, rats were intraperitoneally injected twice with a dose of DEN (50 mg/kg BW). As a promote.,0.05％phenobarbital was fed in drinking water from one week after DEN treatment until the end of experiment. At the end of 8th week, rats were sacrificed and hepatic GST-P$^{+}$ foci, microsomal malondialdehyde (MDA) and conjugated diene contents were determined. In addition, cytochrome P450 content and the activities of NADPH cytochrome P450 reductase and glucose 6-phosphatase were also measured. There was no significant effect by gamma irradiation on microsomal MDA content, conjugated diene, cytochrome P450 content and activities of NADPH cytochrome P450 reductase and glucose 6-phosphatase. However with DEN treatment, microsomal MDA content showed a increasing tendency. Cytochrome P450 content was also significantly increased while microsomal glucose 6-phophatase activity was significantly decreased with DEN treatment. However the activity of NADPH cytochrome P450 reductase was not affected. An interesting finding in this study was that the number and area of hepatic GST-P$^{+}$ foci of rats fed gamma irradiated pork were tended to be decreased by high dose of irradiation, but were not significantly different. These results might imply that the consumption of low dose of gamma irradiated pork does not affect the formation of hepatic GST-P$^{+}$ foci and lipid peroxide and membrane stability.ability.

• 한국어병학회지
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• 제28권1호
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• pp.53-62
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• 2015

Here, we report the physiological changes and growth in disk abalone, Haliotis discus discus, in relation to dietary supplementation with citrus pomace (CP) 6%, Ecklonia cava residue (ECR) 6%, and CP + ECR (3% + 3%). The composition and nutrient content, survival rate and growth rate were measured 0, 4, 8 and 12 weeks after feeding the supplemented diets of CP and/or ECR. Moreover, the experiment of low salinity stress (25psu) for environmental resistance was examined for a period of 48 hours after feeding the supplemented diets for 12 weeks. The activities of superoxide dismutase (SOD), catalase (CAT), lysozymes, respiratory burst, and phenoloxidase were measured. The moisture content and crude protein condition of the body were increased with the addition of ECR only (P<0.05). We observed higher levels of survival in the experimental group compared with the control group. Moreover, the growth disk abalone that were fed a diet containing ECR was higher compared with the control group. However, the growth of abalone fed a diet containing CP was similar to the control group. With a rearing condition of low salinity stress, survival rate and lysozyme activity were increased in the ECR group compared with the control group. Dietary ECR reduced the level of CAT activity to approximately 30% of the control, however the level of CAT activity in the ECR group was similar to the start level of the previous stress. These results suggest that dietary ECR gives rise to an enhanced immunity in disk abalone, as a result of the decrease in CAT and lysozyme activity in particular. Accordingly, the growth and survival rate were increased by feeding an ECR-supplemented diet in the rearing of disk abalone, Haliotis discus discus.

• 한국식품영양과학회지
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• 제28권3호
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• pp.638-645
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• 1999

This study was done to investigate effects of ${\gamma}$ irradiated beef feeding on the formation of gluta thione S transferase placental form positive(GST P+) foci, lipid peroxidation, cytochrome P450 system and microsomal glucose 6 phosphate activity in diethylnitrosamine(DEN) initiated rat hepatocarci nogenesis. Weaning Sprague Dawley male rats were fed the diet containing ${\gamma}$ irradiatied ground beef at the dose of 0, 3, 5kGy as a 20% of protein source for 8 weeks. One week after feeding, rats were intraperitoneally injected twice with a dose of DEN(50mg/kg BW). As a promoter, 0.05% phenobarbital was fed in drinking water from one week after DEN treatment until the end of experiment. At the end of 8th week, rats were sacrificed and hepatic GST P+ foci, microsomal malondialdehyde(MDA) and conjugated diene contents were determined. In addition, cytochrome P450 content and the activities of NADPH cytochrome P450 reductase and glucose 6 phosphatase were also measured. There was no significant effect by gamma irradiation on microsomal MDA content, conjugated diene, cytochrome P450 content and activities of NADPH cytochrome P450 reductase and glucose 6 phosphatase. However with DEN treatment, microsomal MDA content and conjugated diene contents were significantly changed. Cytochrome P450 content was also significantly increased while microsomal glucose 6 phophatase activity was significantly decreased with DEN treatment. However activity of NADPH cytochrome P450 reductase was not affected. An interesting finding in this study was that the number and area of hepatic GST P+ foci of the rats fed gamma irradiated beef were significantly(p<0.05) lower than those of the control. Such a lowering effect on GST P+ foci formation was highest at the dose of 3kGy than others. Overall results suggest that the consumption of low dose of gamma irradiated beef does not affect the formation of lipid peroxide, cytochrome P450 system and membrane stability.

• Fisheries and Aquatic Sciences
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• 제27권9호
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• pp.580-587
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• 2024

Spotted scat Scatophagus argus has a high nutritional value and is among the most widely consumed fish species in Asia. This study was conducted to learn more about the solution for stimulating digestive enzyme synthesis with commercial probiotics. Three different methods of probiotic supplementations of Probai A (Bayer) (feeding - OR, feeding and environment - OE, environment - EN) and control treatment (no probiotic applied) - CT were performed in triplicates by using a completely randomization design method. The results showed no differences in growth performance and survival rates of spotted scat at the fingerling stage among the treatments (p > 0.05). The hepatosomatic index (HSI) and conditional factor did not depend on the probiotic supplementation methods (p > 0.05), but these values had a significant difference between experimental treatments compared to control treatments (p < 0.05). After 15-45 days, only amylase activity in the stomach was significantly higher than those of the control group (p < 0.05). Amylase activity in the intestine and trypsin activity in both the stomach and intestine of experimental treatments were significantly higher than those of the control treatment during 15-30 days (p < 0.05), and then no significant differences were found from day 30 to day 45 (p > 0.05). Our findings indicate that probiotic administration through feeding was the best way to increase the growth weight of spotted scat at the fingerling stage. Further research on the mechanism of stimulating digestive enzyme synthesis when probiotics are used in commercial fish seed production is required.

• Animal Bioscience
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• 제37권2호
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• pp.253-260
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• 2024

Objective: The objectives of the present study were to evaluate the effects of once-a-day milk feeding on growth performance and routine behavior of preweaning dairy calves. Methods: At 22nd day of age, twenty-four Holstein calves were randomly assigned to one of two treatment groups (n = 12/treatment) based on milk feeding frequency (MF): i) 3 L of milk feeding two times a day; ii) 6 L of milk feeding once a day. The milk feeding amount was reduced to half for all calves between 56 and 60 days of age and weaning was done at 60 days of age. To determine the increase in weight and structural measurements, each calf was weighed and measured at 3 weeks of age and then at weaning. The daily behavioral activity of each calf was assessed from the 22nd day of age till weaning (60th day of age) through Nederlandsche Apparatenfabriek (NEDAP) software providing real-time data through a logger fitted on the calf's foot. Results: There was no interaction (p≥0.17) between MF and sex of the calves for routine behavioral parameters, body weight and structural measurements. Similarly, there was no effect of MF on routine behavioral parameters, body weight and structural measurements. However, the sex of the calves affected body weight gain in calves. Male calves had 27% greater total body weight and average daily gain than female calves. There was no effect of the sex of the calves on behavioral measurements. Collectively, in the current study, no negative effects of a once-a-day milk feeding regimen were found on routine behavioral and growth parameters of preweaning calves in group housing. Conclusion: Once-a-day milk feeding can be safely adopted in preweaning calves from 22nd day of age.

• 한국식품영양과학회지
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• 제14권1호
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• pp.77-81
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• 1985

사양기간(飼養期間)이 서로 다른 닭의 골격근에서 actomyosin 을 추출하여 추출성과 생물 활성을 비교하였다. 가슴부위에서 추출한 actomyosin의 추출성은 사양기간(飼養期間)이 3,4,5,6,7주(週)의 순으로 각각 184.5, 364.0, 784.8, 926.1, 985.2, 1020.1 mg/100g 이었고 다리부위는 각각 28.6, 70.8, 137.9, 139.5, 608.3 그리고 646.2mg/100g 이었다. 사양기간(飼養期間)이 3,6,8주(週)인 경우 24시간 추출한 actomyosin의 EDTA-ATPpase활성은 각각 0.68, 0.59, 0.50${\mu}M$ Pi/mg protein min. 이었고 Mg^{+2}$-ATPase 활성은 각각 0.66, 0.71, 0.75 $0.50\;{\mu}M$ Pi/mg protein min.이었고 Mg^{+2}$-ATPase 활성은 각각 0.66, 0.71, $0.75\;{\mu}moles$ Pi-mg protein/min.이었다. 사양기간(飼養期間)에 관계없이 actomyosin 의 3, 6, 8 Mg^{+2}$-ATPase 활성은 저(低) ion 강도에서 높은 활성과 rh(高) ion 강도에서 낮은 biphasic response를 나타내었고 $125\;{\mu}mole$ EGTA로서 Mg^{+2}$-ATPase 활성을 $0.1\;{\mu}mole/mg$ protein/min 이하로 저해시켰다.

• Animal cells and systems
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• 제1권4호
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• pp.571-576
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• 1997

The playas of the Southern High Plains (SHP) of Texas, USA are an important habitat for over one million wintering waterfowl. However, the recent trend toward the modification of playas for agricultural use is threatening winter habitat of waterfowl in this region. Diurnal activity budgets of wintering mallards (Anas platyrhynchos) were conducted from 1 October to 31 March, 1983-1984, and 1984-1985) at three habitat types; steep-sided pits, terraced pits, and open lakes. All seven activity patterns (feeding, locomotion, resting, comfort, courtship, alert, and agonistic) were different (P<0.05) among the three habitat types for wintering mallards on the SHP of Texas. Terraced pits supported more feeding activity (27.8%) (P<0.001) than steep-sided pits (11.2%) or open lakes (2.6%) due to their abundance of natural seeds and aquatic invertebrates. Hens (17.5%) fed more than drakes (11.7%) (P<0.05). Locomotion (32.2%) and alert (2.8%) behavior across the three habitat types showed the highest level during the early morning (6:00-9:00 AM). Paired mallards rested more (37.9%) than unpaired mallards (25.8%) (P<0.05). Agonistic activity was highest (2.4%) in terraced pits throughout the season.

• Journal of Ginseng Research
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• 제37권2호
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• pp.194-200
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• 2013

Korean red ginseng (KRG) is prepared by the process of steaming the roots of Panax ginseng. In this study, the feeding effects of KRG-water extract (KRGE) on ethanol-induced liver damage were elucidated by measuring serum biomarkers in rats. Serum ${\gamma}$-glutamyltranspeptidase (g-GT) activity and the concentration of malondialdehyde (MDA) were significantly increased by short-term and long-term ethanol treatment in rats, whereas the activities of serum glutamate pyruvate transaminase (GPT) and glutamate oxaloacetate transaminase (GOT) did not respond. Pretreatment with KRGE maintained the activity of serum GPT, and the MDA concentration induced by short-term ethanol ingestion remained within the normal range. However, co-feeding of KRGE to rats decreased the concentration of MDA but failed to modulate the serum ${\gamma}$-GT activity induced by long-term ethanol treatment. Our studies suggest that in rats, it appears that KRGE does not sufficiently reverse the physiological response evoked by long-term ethanol ingestion to maintain normal conditions, in view of the serum biomarker ${\gamma}$-GT, regardless of KRGE's favorable antioxidant activity.

• 한국식품영양과학회지
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• 제23권6호
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• pp.879-886
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• 1994

This study was carried out to determine, the effects of sodium alginate and cellulose on the plasma lipoportein composition and cholesterol metabolism inrats.Each experimental diet contained 105 sodium alginate and cellulose by weight, respectivley and rats were fed fro 4 weeks. The results obtained were as follows : The feeding of sodium alginate and cellulose decreased total plasma cholesterol slightly . total cholesterol of Chylomicron /VLDL- , LDL-fraction and liver were decreased significantly insodium alginate group. HDL-cholesterol was slightly increased in soidum alginate group. The feeding of sodium alginate significantly lowered plasma , Chylomicron VLDL-, LDL-fraction and liver TG concentrations compared with those fed fiber-free diet . The HMG-CoA reductase activity was not different among diet groups but the lowest activity was observed in sodium alginate group. The feeding of sodium alginate significantly increased fecal cholesterol , TG, and bile acid excretion . In summary , the ingestion of sodium alginate decreased cholesterol and TG concentrations of plasma and liver. This may be explained by the facts that fecal cholesterol, bile acid and TG level were increased significantly in sodium alginate group.

• 한국가금학회지
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• 제23권2호
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• pp.85-98
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• 1996

Results of experiments on the nutrient requirements and feeding system of broiler breeder hens were reviewed, and daily requirements of energy and protein were calculated using the prediction equations reported by Scott(1977) and NRC(1981). The experimental reports on daily ME needs of broiler breeder hens were ranged from 400 to 450 kcal, however, the ME needs of caged hens were 92~93% to those of floor-housed hens due to the difference of ME need for activity. The ME needs of broiler breeders decreased with increasing environmental temperature corresponding to a drop of 25 kcal per day for each 5˚C rise. About 80~90% of the daily ME needs were used for body rnaintenance and activity of hens. Experimental results on daily protein needs of broiler breeder hens were ranged from 18 to 22 g, however, calculated protein needs decreased as the BW gain and eggmass output decreased after peak production, and about 60~65% of the daily protein needs were used for egg production. In the current practice, broiler breeder hens are restricted in feed, and consume their daily allowance in the first 2 to 6 h after dawn. The results suggest that eggshell quality can be significantly improved in hens fed during the afternoon when shell calcification is initiated, with no adverse effect on laying rate and fertility of eggs.