Effects of $\gamma$-Irradiated Beef Feeding on Preneoplastic Hepatic Lesion, Cytochrome P450 System and Microsome Glucose 6-Phosphatase Activity in Rat Hepatocarcinogenesis

실험적 간 발암모델에서 감마선 조사 쇠고기 섭취가 전암성병변의 생성, 약물대사 효소계 및 소포체 막 안정성에 미치는 영향

  • 김정희 (서울여자대학교 영양학과) ;
  • 김미정 (서울여자대학교 영양학과) ;
  • 강일준 (한림대학교 식품영양학과) ;
  • 변명우 (한국원자력연구소)
  • Published : 1999.06.01


This study was done to investigate effects of ${\gamma}$ irradiated beef feeding on the formation of gluta thione S transferase placental form positive(GST P+) foci, lipid peroxidation, cytochrome P450 system and microsomal glucose 6 phosphate activity in diethylnitrosamine(DEN) initiated rat hepatocarci nogenesis. Weaning Sprague Dawley male rats were fed the diet containing ${\gamma}$ irradiatied ground beef at the dose of 0, 3, 5kGy as a 20% of protein source for 8 weeks. One week after feeding, rats were intraperitoneally injected twice with a dose of DEN(50mg/kg BW). As a promoter, 0.05% phenobarbital was fed in drinking water from one week after DEN treatment until the end of experiment. At the end of 8th week, rats were sacrificed and hepatic GST P+ foci, microsomal malondialdehyde(MDA) and conjugated diene contents were determined. In addition, cytochrome P450 content and the activities of NADPH cytochrome P450 reductase and glucose 6 phosphatase were also measured. There was no significant effect by gamma irradiation on microsomal MDA content, conjugated diene, cytochrome P450 content and activities of NADPH cytochrome P450 reductase and glucose 6 phosphatase. However with DEN treatment, microsomal MDA content and conjugated diene contents were significantly changed. Cytochrome P450 content was also significantly increased while microsomal glucose 6 phophatase activity was significantly decreased with DEN treatment. However activity of NADPH cytochrome P450 reductase was not affected. An interesting finding in this study was that the number and area of hepatic GST P+ foci of the rats fed gamma irradiated beef were significantly(p<0.05) lower than those of the control. Such a lowering effect on GST P+ foci formation was highest at the dose of 3kGy than others. Overall results suggest that the consumption of low dose of gamma irradiated beef does not affect the formation of lipid peroxide, cytochrome P450 system and membrane stability.



  1. Technical report series No. 695 wholesomeness of irradiation food WHO
  2. Food Sci. Industry v.30 Application and aspect of irradiation techonology in food industry Byun, M. W.
  3. Appl. Envir. Microbiol. v.59 Elimination of Escherichia coli O157:H7 in meats by gamma irradiation Thayer, D. W.;Boyd, G.
  4. Korean J. Food Sci. Technol. v.30 Sterilization of Escherichia coli O157:H7 contaminated beef by gamma irradiation Kim, S.;Yook, H. S.;Lee, J. W.;Choi, C.;Byun, M. W.
  5. Korean J. Food Sci. Technol. v.30 Changes of physicochemical properties of gamma irradiated porks during storage Kwak, H. J.;Kang, I. J.;Kim, H. K.;Chang, H. G.;Park, S. A.
  6. J. Agric. Food Chem. v.23 Chemical chages associated with flabor in irradiated meat Merritt, C.;Angelini, P.;Wierbicki, E.;Shults, G. W.
  7. Korean J. Food Sci. Technol. v.30 Effects of gamma irradiation on pigments of beef Yook, H. S.;Lee, J. W.;Lee, K. H.;Kim, S.;Byun, M. W.
  8. J. Food Technol. v.20 Effect of low-dose irradiation on the shelf life of beefburgers stired at 3℃ Dempster, J. F.;Hawrysh, Z. J.;Shand, P.;Lahola-Chomiak, L.;Corletto, L.
  9. J. Food Sci. v.51 Effect of low dose irradiation of pork loins on the microflora sensory characteristics and fat stablity Mattison, M. L.;Kraft, A. A.;Olson, D. G.;Walker, M. W.;Rust, R. E.;James, D. D.
  10. Food Technol. Aust. v.40 The use of irradiaton for extending the storage life of fresh and processed meats Shay, B. J.;Egan, A. F.;Wills, P. A.
  11. Food Technology Wholesomeness of irradiated foods Thayer, D. W.
  12. J. Biol. Chem. v.264 Protein kinase C located in rat liver nuclei Masmoudi, A.;Labourdette, G.;Mersel, M.;Huang, F. L.;Huang, K. P.;Vincendon, G.;Malviya, A. N.
  13. J. Histochem. Cytochem. v.29 Use of avidinebiotin peroxidase complex complex(ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody(PAP) procedure Hsu, S. M.;Raine, L.;Fanger, H.
  14. Methods Enzymol. v.105 Spectrophotometric detection of lipid conjugated dienes Rechnagel, R. O.;Glende, E. A.
  15. Lipids v.8 Damage to microsomal membrane by lipid peroxidation Bidlack, W. T.;Tapple, A. L.
  16. J. Biol. Chem. v.239 The carbon monoxide binding pigment of liver microsome Omura, T.;SAto, R.
  17. Methods of enzymatic analysis (2nd ed.) Glucose-6-phosphatase Baginiski, E. S.;Foa, P. P.;Zak, B.
  18. J. Biol. Chem. v.193 Protein measurement with the Folin-Phenol reagent Lowry, O. H.;Rosebrough, N. J.;Farr, A. L.;Randall, R. T.
  19. Korean J. Food Sci. Technol. v.30 Genotoxical and acute toxicological sageties of gamma irradiated beef Kang, I. J.;Kwak, H. J.;Lee, B. H.;Kim, K. H.;Byun, M. W.;Yook, H. S.
  20. Federation Proc. v.15 Wholesomeness of γ-irradiated diet fed to chickens Burns, C. H.;Brownell, L. E.;Echstein, H. C.
  21. Federation Proc. v.19 Effect of irradiated meat upon growth and reproduction of dogs McCay, C. M.;Rumsey, G. L.
  22. J. Food Sci. Nutr. v.1 Effects of sardine oil feeding and vitamin E supplement on the preneoplastic hepatic lesion and cholesterol metabolism in hepatocarcinogenesis of rats Kim, J. H.;Yoon, H. J.;Jang, J. J.
  23. J. Korean Assoc. Cancer Prev. v.1 Anticarcinogenic effects of d-limonene on DEN-induced rat hepatocarcinogenesis system Kim, J. H.;Ju, E. S.;Lee, M. S.;Jang, J. J.
  24. Bull. Exp. Biol. Med. v.87 Metabolism of lipid peroxide during chemical carcinogenesis Lankin, V. Z.;Polyakov, V. M.;Askhangel, S. V.;Gurevich, S. M.
  25. CRC Critical REv. Toxicol. v.18 Lipid peroxidation and mechanism of toxicity Horton, A. A.;Fairhust, S.
  26. Free Radic. Res. v.28 Measurement of lipid peroxidation Roberts, M. K.
  27. J. Sci. Food Agric. v.11 Treatment of meats with ionizing radiations-V. Radiation pasteurization of beef for chiled storage Lea, C. H.;Macfarlane, J. J.;Parr, L. J.
  28. Meat Sci. v.36 Improvement of shelf-life and sholesomenesss of ground beef by erradiation-2. Chemical analysis and sensory evaluation Lefebvre, N.;Thibault, C.;Charbonneau, R.;Piette, J. P. G.
  29. Biochim. Biophys. Acta. v.620 Lipid peroxidation of rat liver microsomes Koster, J. F.;Slee, R. G.
  30. Korean J. Food Sci. Technol. v.30 Effects of γ-irradiation on meat proteins Yook, H. S.;Kim, M. R.;Kim, J. O.;Lim, S. I.;Byun, M. W.