• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1053-1056
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• 2011

Optimal conditions for a high cell density fermentation were investigated in a recombinant Escherichia coli producing salmosin, a platelet aggregation inhibitor. The optimized carbon and nitrogen sources were glycerol 10 g/l, yeast extract 30 g/l, and bacto-tryptone 10 g/l, yielding the dry cell weight (DCW) of 10.61 g/l in a 500 ml flask culture. The late-stage induction with 1% L-arabinose in a 5 l jar fermentor showed the highest DCW of 65.70 g/l after 27 h of the fed-batch fermentation. Around 2,200 mg/l of the protein was expressed as an inclusion body that was then refolded to obtain the active salmosin of 96 mg/l. We also confirmed the inhibitory activity against platelet aggregation of the active salmosin from the high cell density fermentation.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.340-345
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• 2010

The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Gluconohacter oxydans ZJB09112 in shake flasks and bubble column bioreactors. After fermentation in the optimized medium (g/l: yeast extract 5, glycerol 2.5, mannitol 22.5, $K_2HPO_4$ 0.5, $KH_2PO_4$ 0.5, $MgSO_4{\cdot}7H_2O$ 0.1, $CaCO_3$ 2.0, pH 5.0), when five times of glycerol feeding were applied, $161.9{\pm}5.9\;g/l$ of DHA was attained at a $88.7{\pm}3.2%$ conversion rate of glycerol to DHA.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.65-68
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• 2003

One of the biggest problems encountered during the last phase of silkworm rearing is non-uniform maturation of the silkworms especially during cooler months. Phytoecdysteroid (20-hydroxy ecdysone) was extracted in large-scale from a plant belongs to Caryophyllaceae and fed to silkworm larvae to test the effect of phytoecdysteroid. About 80％ of the silkworms were ready for mounting by 18 hrs after treatment (when the treatment is done for uniform spinning), whereas in control batch only 37％ worms were ready for mounting by the same time.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.376-379
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• 2000

The methylotrophic yeast Pichia pastoris is one of the best host for the production of foreign proteins because of the presence of the strong AOX1 promoter induced by methanol. Methanol feeding induces the protein production and provides energy sources for the host cells. However, excess methanol inhibits the growth of host cells, while an insufficient methanol lead to poor growth and protein production. We have used various controled methanol feeding strategies to obtain the maximum proteins.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.245-246
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• 2000

Agrobacterium sp. ATCC 31750( formerly Alcaligenes faecalis subsp myxogenes) was used to produce curdlan. Since the curdlan is secondary metabolite, it is important for curdlan production to increase cell concentration. The fedbatch operation was used to increase cell concentration with addition of carbon and nitrogen sources. When the initial sucrose concentration was 20g/L, it was consumed in 24 hrs and the cell concentration was 6g/L in a batch culture. The sucrose solution(200g/L) was fed to control the sucrose concentration above 10g/L.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.260-265
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• 2005

In the previous research about ethanol production, we confirmed that SFW(saccharified foodwastes) medium(0.56g-ethanol/g-glucose) is mere efficient than YM medium(0.538g-ethanol/g-glucose). Ethanol production using SFW needs large enzyme cost due to the enzymatic hydrolysis of foodwastes, although the enzymes was obtained from our economical enzyme production methods, using the intact whole culture broth of Trichoderma harzianum FJ1. Therefore, in this research we used synchronous saccharification and fermentationmethod to produce ethanol using foodwastes. Ethanol production yield was 0.45g-ethanol/g-reducing sugar in synchronous saccharification and for-mentation by a fed-batch mode.

• Journal of Environmental Health Sciences
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• v.33 no.6
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• pp.493-496
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• 2007

The influences of contact time and ratio of food to microorganism (F/M) on uptake of wastewater organic matter in a short contact process were investigated using three activated sludge batch reactors fed with synthetic wastewater, sewage and livestock wastewater. About 64% of influent soluble chemical oxygen demand (SCOD) in the synthetic wastewater and 61% of SCOD in the sewage and 43% of SCOD in the diluted livestock wastewater were adsorbed into the activated sludge within 30 min. The specific mass of organic matter uptaken in the synthetic wastewater was 55 mg SCOD/g mixed liquor suspended solids (MLSS). In the same manner, 20 and 14 mg SCOD/g MLSS were calculated as the values in the sewage and livestock wastewater, respectively.

• Textile Coloration and Finishing
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• v.7 no.1
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• pp.51-57
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• 1995

Poly-$\beta$-hydroxybutyrate(PHB) was biosynthesized using Alcaligenes sp. FL-027. Alcaligenes sp. FL-027 was cultivated by fed-batch methods, in order to promote cell growth and PHB accumulation with carbon source. The cells were first grown at 3$0^{\circ}C$ on the fermentor. The structure of biosynthesized PHB is investigated by the NMR, IR. The crystalline portions were identified through the use of DSC and X-ray diffractometer. The melting point was about 16$0^{\circ}C$ and the diffraction peaks of (020) and (110) were shown at 13$^{\circ}$ and 17$^{\circ}$, respectively.

• 제어로봇시스템학회:학술대회논문집
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• 1990.10a
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• pp.275-280
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• 1990

A neural network method is applied for recognizing the orientation o f individual parts being fed from a bowl feeder. The system is designed in such a way that a part can be discriminated and sorting according to every possible stable orientation without implementing any a mechanical tooling. The operation of the bowl feeder is based on a 2D image obtained from an array of fiber optic sensor located on the feeder track. The acquired binary image of a moving and vibrating part is used as input to a neural network which, in turn, determines t he orientation of the part. The main task of the neural network, here is to synthesize the appropriate internal discriminant functions for the part orientation using the part features. A series of the experiments reveals several promising points on performance. Since the operation of the feeder is highly programmable, it is well suited for feeding and sorting small parts prior to small batch assembly work.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.4
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• pp.242-246
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• 2000

Human lipocortin-I was expressed as a secretory product by Saccharomyces cerevisiae harboring an expression system consisting of GAL10 promoter, inulinase signal sequence and lipocortin-I terminator. Fed-batch fermentation was carried out to overproduce recombinant human lipocortin-I. The culture medium was desalted and concentrated by ultrafiltration, and then subjected to hydroxyapatite column chromatography. The lipocortin-I was purified to >98% purity by single-step hydroxyapatite column chromato-graphy. However, it was found that the purified lipocortin-I was a proteolytically-cleaved form which was cleaved immediately after the basic amino acid Lys26.