• 융합정보논문지
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• 제11권4호
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• pp.111-121
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• 2021

감국의 유산균 발효를 통한 감국 배양물(CILL)의 비만억제 식품의 소재로서 가능성을 알아보기 위해 고지방식이로 유도된 C57BL/6 mice에서의 체중, body fat mass, T cell 등을 측정하였다. 체중 변화에서 CILL 군 (25.15±2.44 g)은 4주부터 체중 감소를 보였으며, 1주차부터 8주차까지 낮은 증체량을 유지했다(1.00±0.53 g). 8주 체중(30.38±4.17 g)은 HFD 군(60% high fat diet, 34.99±2.09 g) 대비 13.15%의 체중 감소를 보였다. Fat mass는 10.3022±2.8813 g로 감소했으며, 간 절대 중량에서 HFD 군에 비해 감소하였다. CD4+ T cell 4.84±1.33%, CD8+ T cell 7.02±2.26%, CD4+CD8+ T cell 1.46±0.81%로 HFD 군에 비해 높게 측정되었다. 결과적으로 CILL은 비만억제 효과가 있으며, CILL내의 비만억제 물질을 선별한다면 효과적인 비만억제 식품 소재로서의 가능성이 있을 것으로 사료된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권1호
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• pp.22-29
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• 2008

Purpose: The purpose of this study is to test the efficacy of various methods of fat harvesting in animal model by viability comparison with assay including cell counting, MTT assay, and histologic evaluation. Materials and methods: New Zealand white rabbits experiments were used. Groin fat pads were subjected to different harvest method varying ingredients of solution(Experiment 1: T1 solution= lidocaine 1000mg/L, epinephrine 1mg/L, sodium bicarbonate 10mgEq/L, Triamcinolone 10mgEq/L; T2 solution=lidocaine 1000mg/L, epinephrine 1mg/L, sodium bicarbonate 0mgEq/L, Triamcinolone 0mgEq/L) and pressure exerted on harvesting with Luer-Lock syringe connected to suction cannula.(Experiment 2: P1 group=3cc intermittent pressure; P2 group=10cc sustained pressure) Fat cell viability was assessed with cell counting with a hemocytometer, MTT assay, and histologic evaluation. Results: Experiment 1 Cell count: T1=2.4/3.4/4.2, T2=9.6/8.4/7.2($\times10^5$ per mL); MTT assay: T1=0.516/0.41/0.453/0.412/0.421, T2=0.925/0.765/0.54/0.634/0.614 in 21 days(absorbance); Histology: T1 showed elongated and, different in size and shape, and ruptured adipocytes with only a few normal adipocytes whereas T2 showed central core of fat with almost intact fat cells Experiment 2 Cell count: P1=1.2/3.2/4.2, P2=1.2/2.4/3.8($\times10^5$ per mL); MTT assay:P1=0.256/0.245/0.258/0.21/0.264, P2=0.12/0.231/0.245/0.313/0.281 in 21 days(absorbance); Histology: P1 showed somewhat evenly distributed normal-looking fat cells and P2 showed relatively irregular shape of fat cells with small blood vessel amongst adiopocytes. Conclusion: Viability was higher in ‘modified tumescent solution’without sodium bicarbonate and triamcinolone and we also found no significantly different viability between using intermittent pressure and using sustained pressure. But in terms of initial viability of fat cell, we can assume that lower intermittent pressure would make better clinical results.

• Journal of Nutrition and Health
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• 제17권4호
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• pp.253-261
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• 1984

식이중 지방 수준과 fiber 종류가 흰 쥐의 혈청과 간 조직내의 지질 함량에 미치는 영향을 알아보기 위해 지방 수준을 0% , 5% , 20% 수준으로 달리 하였으며 fiber 종류는 고지방 식이군( 20% 지방 )에 pectin과 cellulose를 각각 10% 수준으로 첨가하였다. 또 식이내 cholesterol첨가의 효과를 관찰하기 위해 고지방 식이군에 0.5% cholesterol을 첨가한 후 pectin과 cellulose를 10%씩 넣어 전체적으로 7군으로 나누어 각 각 다른 식이로 4주간 사양한 후 혈청과 간에서 지질 함량을 조사하였으며 간 조직의 형태학적 조사를 실시하여 다음과 같은 결과를 얻었다. 1) 식이 섭취량은 지방 수준이 낮을수록 높았으나 뚜렷한 차이는 보이지 않았으며 pectin과 cellulose 첨가로 인해서 섭취량이 줄었다. 증체량은 지방 수준이나 fiber종류에 따라 큰 차이를 보이지 않았다. 변의 무게는 지방 수준에 따른 차이는 없었으나 fiber 첨가로 인해서는 pectin은 약간의 감소를 cellulose 는 뚜렷한 증가를 보였다. 또 cholesterol을 첨가한 군 간의 비교에서도 pectin첨가군은 감소를 cellulose첨가군은 증가를 나타냈다. 간과 epididymal fat pad의 무게는 지방 수준이 높아짐에 따라 증가하였으며 fiber 종류에 따라서는 pectin첨가군이 cellulose 첨가 군 보다 낮았다. 2) 혈청 분석 결과는 식이내 지방 수준이 높을수록 total lipids수준이 높아지는 경향을 보였으며 pectin이 cellulose보다 혈청내 지질 수준을 뚜렷하게 낮추었다. Total cholesterol 및 phospholipids 량에 있어서도 지방 수준이 증가함에 따라 높아졌으며 cellulose보다 pectin이 첨가 되었을때 현저한 감소를 나타냈다. HDL- cholesterol수준은 무지방군에서는 매우 높게 나타났으나 표준군과 고지방군간에 는 명확한 결과를 얻을 수 없었다. 3) 간 분석 결과는 지방수준이 높을수록 total lipids량이 높아지는 경향을 보였다. 또 pectin 첨가가 cellulose첨가군보다 현저히 낮은 lipids수준을 나타냈다. 간의 total cholesterol함량은 지방 수준이 높아짐에 따라 증가하였으나 뚜렷한 차이는 아니었으며 pectin첨가가 cellulose첨가보다 cholesterol 수준이 상당히 낮았다. 4) 간 조직의 현미경 관찰 결과는 지방 수준이 높아짐에 따라 조직내 지방 침착이 심해졌으며 fiber 의 첨가로 인해서는 pectin은 뚜렷한 지방 침착의 감소를 보였으나 cellulose는 거의 감소 효과를 보이지 않았다.

• 대한추나의학회지
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• 제3권1호
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• pp.15-29
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• 2002

Objectives : This experimental study was designed to investigate the effects of Galgunseunggi-tang on the weight of obese rats Induced by high fat diet. Methods : A series of have been conducted in order to measure the effects of the above materials weight. serum and fat cells. The have been performed on; (1) the Increasing amount of body weight; (2) the quantity of total cholesterol, tryglyceride, total lipid, free fatty acid, phospholipid, HDL -cholesterol and LDL -cholesterol in serum; (3) the average size of epididymal fat cells and the area rate of fat drops in hepatic lobule. Results : The results were summarized as follows: 1. It was proved that Galgunseunggi-tang suppressed the increasing amount of body weight. GS II group (supplied 60mg per 100g) showed a significant value. 2. It was proved that Galgunseunggi-tang improved the total cholesterol, HDL -cholesterol In serum. but did not show a significance. 3. It was proved that Galgunseunggi-tang decreased the quantify of free fatty acid, phospholipid, LDL -cholesterol in serum. GS I group (supplied 30mg per 100g) showed a significant value. 4. It was proved that Galgunseunggi-tang decreased the quantity of triglyceride, total lipid in serum, and GS II group showed a significant value. 5. Galgunseunggi-tang decreased the average size of epididymal fat cells and the area rate of fat drops In hepatic lobule, and GS I group showed a significant value. Conclusions : Based on the above result, it is assumed that the clinical application of Galgunseunggi-tang can help the treatment of obesity.

• 대한의생명과학회지
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• 제25권3호
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• pp.227-236
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• 2019

In this study, the anti-oxidant, anti-inflammatory, and anti-obesity activities of an ethanol extracts of Ulmus divididiana var. japonica (UDE) were investigated in vitro and in vivo. UDE anti-oxidant activity was evaluated with an Electron Spin Resonance (ESR) spectrometer, which measured 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. Cell viabilities were estimated using 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. LPS-stimulated BV-2 microglia were used to study the production of nitric oxide (NO). Cells stimulated with LPS produce more NO than normal control cells. However, cells treated with the UDE decreased this production in a concentration dependent manner (100, 250, 500, $1,000{\mu}g/mL$). Also, we investigated the anti-obese activity of UDE in SD rats. The SD rats were randomly divided into five groups: 10% low fat diet (N), 45% high fat diet (H), 45% high fat diet + garcinia extracts 200 mg/kg/day (HG200), high fat diet + UDE 200 mg/kg/day (HU200), high fat diet + UDE 400 mg/kg/day (HU400). UDE was found to lower whole body and abdominal and epididymal adipose tissue weights and lowered plasma levels of triglyceride (TG), compared to those in H group. Histological analyses of the liver and fat tissues of rat treated with UDE revealed significantly decreased number of lipid droplets and decreased size of adipocytes compared to the H group. These results suggest that UDE might be used to develop potent anti-oxidant, anti-inflammatory, and anti-obesity agents, and may be useful as ingredients for related new functional raw materials.

• Journal of Ginseng Research
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• 제44권2호
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• pp.350-361
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• 2020

Background: Black ginseng (BG) is a type of Korean ginseng prepared by steaming and drying raw ginseng to improve the saponin content. This study examined the effects of BG on nonalcoholic fatty liver disease (NAFLD) in HepG2 cells and diet-induced obese mice. Methods: HepG2 cells were treated with free fatty acids to induce lipid accumulation before supplementation with BG. NAFLD-induced mice were fed different doses (0.5%, 1%, and 2%) of BG for 8 weeks. Results: BG significantly reduced lipid accumulation and expression of lipogenic genes, peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, sterol regulatory element-binding protein-1c, and fatty acid synthase in HepG2 cells, and the livers of mice fed a 45% high-fat diet with 10% fructose in the drinking water (HFHF diet). BG supplementation caused a significant reduction in levels of aspartate aminotransferase and alanine aminotransferase, while antioxidant enzymes activities were significantly increased in 45% high-fat diet with 10% fructose in the drinking water diet-fed mice. Expression of proliferator-activated receptor alpha and carnitine palmitoyltransferase I were upregulated at the transcription and translation levels in both HepG2 cells and diet-induced obese mice. Furthermore, BG-induced phosphorylation of AMP-activated protein kinase and acetyl CoA carboxylase in both models, suggesting its role in AMP-activated protein kinase activation and the acetyl CoA carboxylase signaling pathway. Conclusion: Our results indicate that BG may be a potential therapeutic agent for the prevention of NAFLD.

• 대한한방소아과학회지
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• 제29권2호
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• pp.37-48
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• 2015

Objectives This experimental study was designed to investigate the antiobese effects of Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan. Methods The cellular lipid contents were assessed by Oil-Red-O staining. The expression of $PPAR{\gamma}$ and $C/EBP{\alpha}$ were determined by real time RT-PCR and western blotting. In addition, body weight gain and serum lipid levels were measured in the mice with obesity induced by the high fat-diet for four weeks. Results Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan is reduced 3T3-L1 cells' differentiation and the expressions of $PPAR{\gamma}$ and $C/EBP{\alpha}$ in high concentration group. High-fat diet + Fermented GamiKwakhyangjungkisan group significantly reduced body weight gain. High-fat diet + Fermented GamiKwakhyangjungkisan group significantly increased HDL-cholesterol contents and reduced LDL-cholesterol contents. Furthermore, Fermented GamiKwakhyangjungkisan is excellent antiobese effects than Gamikwakhyangjungkisan. Conclusions These results demonstrate that Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan exerts antiobese effect in 3T3-L1 cells and mice fed high fat diet. Furthermore, Fermented GamiKwakhyangjungkisan is excellent antiobese effects than Gamikwakhyangjungkisan.

• 대한세포병리학회지
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• 제19권2호
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• pp.200-205
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• 2008

Hibernoma is a rare, benign adipose tumor composed of brown fat cells with eosinophilic granular or multivacuolated cytoplasm. The cytologic features of hibernoma have been rarely reported and may mimic other polygonal cell neoplasms. We report the imprint cytologic features of a case of hibernoma in the left thigh of a 68-year-old woman. Microscopic examination showed large, round, or polygonal brown fat cells. The cells were arranged in fragments or clusters. The nuclei were uniformly round with finely granular chrornatin. The cytoplasm was multivacuolated or univacuolated. The abundant eosinophilic granular cytoplasm was also present. No nuclear atypia were present. Immunohistochemical staining showed that cells were positive for S-100 protein.

• Archives of Plastic Surgery
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• 제39권6호
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• pp.659-662
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• 2012

Progressive facial hemiatrophy, also known as Parry-Romberg syndrome, is a progressive and self-limited deformation of the subcutaneous tissue volume on one side of the face that creates craniofacial asymmetry. We present the case of a patient with a five-year history of progressive right facial hemiatrophy, who underwent facial volumetric restoration using cell-assisted lipotransfer (CAL), which consists of an autologous fat graft enriched with adipose-derived stem cells (ASCs) extracted from the same patient. ASCs have the capacity to differentiate into adipocytes. They also promote angiogenesis, release angiogenic growth factors, and some can survive as stem cells. The use of autologous fat as a filler in soft tissue atrophy has been satisfactory in patients with mild and moderate Parry-Romberg syndrome. Currently, CAL has showed promising results in the long term by decreasing the rate of fat reabsorption. The permanence and stability of the graft in all the injected areas has showed that autologous fat grafts enriched with stem cells could be a promising technique for the correction of defects caused by this syndrome.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권4호
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• pp.412-418
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• 2008

Bone morphogenetic proteins (BMPs) in combination with stem cells gain more significance for their use in bone tissue engineering. The mesenchymal stem cell can be differentiated into osteoblast by the treatment of BMP. The aim of this study is to characterize the osteogenic differentiation process of adult stem cells derived from buccal fat pad according to BMP-2 within culture media and decide the appropriate concentration of BMP-2 to facilitate osteogenesis. The authors procured the stem cell from buccal fat pad and analyzed for presence of stem cell by flow cytomety against CD-34, CD-105 and STRO-1. The buccal fat derived stem cells (BFDC) were treated by application of the different concentration with BMP-2 of 0, 10, 50, 100 and 200ng/ml, respectively. And their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase(ALP) staining, Alizarin red staining and RT-PCR for osteocalcin(OC) gene expression at 7, 14 and 21day of culture. Flow cytometric analysis and biochemical assays demonstrated that BFDC might be a distinguished stem cells, and mineralization was accompanied in proportion to BMP-2 concentration. However, with 100ng/ml concentration of BMP-2, the BFDC demonstrated most efficient staining pattern of ALP and Alizarin red. The feasibility of the osteogenic differentiation in the group of both 50ng/ml and 200ng/ml of BMP-2 showed similar activity and relatively weaker than that of 100ng/ml. These results suggest that the BMP-2 stimulate osteogenesis by BFDC effectively and that bone induction might be controlled through negative regulatory feedback in higher concentration.