Defatted rice bran was microparticulated using fluidized bed opposed jet mill and air-classified at different air classifying wheel speed (ACWS) in Turboplex classifier. The median particle size and the standard deviation decreased, and concomitantly the specific surface area increased generally with increasing ACWS. The protein, fat and ash contents of the recovered rice bran increased with ACWS. The contents of minerals; magnesium, zinc, iron and manganese; increased positively with ACWS. The phytic acid content, however, was slightly higher at middle ACWS. The dietary fiber content was highest in the ACWS 15,000 rpm fraction showing 31.47%. Higher ACWS resulted in lighter colored powder. The water holding capacity (WHC) showed the maximum value at ACWS 12,000 rpm and decreased with increasing ACWS, while the oil holding capacity (OHC) increased with ACWS. The rheological property of the microparticulated rice bran/water suspension fitted to the linear model. The yield stress and viscosity of the suspension increased with ACWS. The shape of microparticulated rice bran at ACWS 21,000 rpm was spherical, and the median particle size was $3.7{\mu}m$. When cake was prepared with substitution of microparticulated rice bran at 5%, the cake height and volume increased remarkably.
Chee, Kew M.;Chun, Kwang S.;Huh, Bong D.;Choi, Jin H.;Chung, Mahn K.;Lee, Hyung S.;Shin, In S.;Whang, Kwang Y.
Asian-Australasian Journal of Animal Sciences
/
v.18
no.6
/
pp.861-867
/
2005
Feeding values of soybean hulls (SH) were compared to those of wheat bran (WB) for swine diets by chemical compositions, a digestion trial, a preference test by self-selection, and two feeding trials. The SH and the WB appeared to have, on airdry basis, 11.1 vs. 15.4% CP, 32.5 vs. 8.7% crude fiber (CF), 36.8 vs. 10.7% ADF, 0.6 vs. 0.1% Ca, and 492 vs. 92 ppm Fe, respectively. Lysine and total sulfur-containing amino acids in the SH were 0.66 vs. 0.37%, respectively. Apparent digestibility values of the SH were 71% for dry matter, 50% for CP, and 74% for CF. Apparent digestible energy and MEn values of the SH were 2,420 and 2,370 kcal kg$^-1$, respectively, which were comparable to those of the WB, 2,420 and 2,275 kcal kg$^-1$ (NRC, 1998), respectively. The first feeding trial was conducted with 72 crossbred growing pigs with an average weight of 29.6 kg. The pigs when fed the diets containing 0, 6 and 10% SH by replacing the WB on a weight basis for 42 days did not show significant differences in body weight gain and feed/gain ratio among the treatments. The same trends were observed in the second trial with 60 crossbred finishing pigs with an average weight of 64.5 kg when fed the diets containing 12% SH or WB for 41 days. Back-fat thickness and adjusted loin eye muscle area of the finisher pigs were also not significantly different between the two groups. When allowed to self-select from two different feed troughs containing 10% SH or WB for two weeks, two groups of 80 pigs with 10 pigs per pen consumed the two diets exactly in equal proportion. In conclusion, the soybean hulls can be included up to 10 and 12% for growing or finishing pig diets, respectively, replacing the wheat bran on a weight basis without any adverse effects on palatability of diets and animal performances.
Malate dehydrogenase in the mosquito ovary after a blood meal, Aedes aegypti, was purified and characterized. MDH purification steps involved DEAE-Sepharose, S-Sepharose and Cibacron blue affinity chromatography. The purified MDH was 70,000 daltons in molecular weight and was a homodimer consisting of tow identical subunits. Optimal activity of purified MDH was obtained pH 9.0-9.2 in malate-oxaloacetate reaction and pH 9.8-10.2, in oxaloactate-malate reaction. With obtained pH 9.0-92 in malate-oxaloacetate reaction and pH 9.8-10.2, in oxaloactate-malate reaction. With malate as substrate, purified mitochondrial MDH (1.28$\times$${10}^{-4}$ M) had lower Km value than cytoplasmic MDH (8.92x${10}^{-3}$ M). MDH activity was inhibited by citrate, $\alpha$-ketoglutarate, and ATP. Inhibition of MDH activity by ATP and citrate was less in malate-oxaloacetate reaction and in oxaloacetate-malate reaction. MDH activity was completely inhibited by ATP in oxaloacetate-malate reaction and not inhibited by citrate in malate-oxaloacetate reaction. Temporal activity change of MDH is similar to that of isocitrate dehydrogenase in the ovary after blood feeding; their activities in the ovary began to rise at 18 hours after a blood meal, and reached at the maximal level at 48 hours.
Kim, Yiseul;Jeon, Byungduk;Choi, Seokrip;Kim, Woocheol;Lee, Dong Woon;Ryu, Sungpil
Korean journal of applied entomology
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v.55
no.1
/
pp.53-62
/
2016
The purpose of this study was to analyze the effect of grasshopper (Oxya chinensis sinuosa) powder ingestion with/without aerobic exercise (treadmill running) on energy metabolism. To achieve this purpose, 28 Institute of Cancer Research (ICR) mice were divided into four groups: normal diet control group (CON), a normal diet with exercise control group (COEX), a grasshopper powder-supplemented diet group (GH), and a grasshopper powder-supplemented diet with exercise group (GHEX). Duration of the powder ingestion and aerobic exercise training were 6 weeks. Body weight gain ratio was not significant. Fat mass significantly decreased in GH and GHEX. There were no changes in blood glutamic oxaloacetic transaminase and glutamic pyruvic transaminase levels between groups. Glucose transporter type 2 and glucose transporter type 4 protein levels were not significantly different between groups. Fibronectin type III domain-containing protein 5 level was the highest in GHEX. AMP-activated protein kinase level significantly increased in GHEX compared to the levels in the other groups. Glycogen synthase kinase 3 beta protein level was reduced in GHEX compared to that in CON. These results suggest that grasshopper powder ingestion and endurance exercise training influence energy metabolism.
Some high frequency sounds alter physiological processes of the beet armyworm, Spodoptera exigua. This study investigated the effect of ultrasound (${\geq}$ 20 kHz) on larval feeding, pupal development, and adult mating behavior of S. exigua. Ultrasound suppressed feeding behavior of fifth instar larvae, and 30 or 45 kHz treatment inhibited more than 50% of feeding activity. Larvae treated with ultrasound exhibited alterations in major nutrient compositions in the hemolymph plasma. Plasma protein levels decreased with an increase in ultrasound frequency. In contrast, sugar levels increased with an increase in ultrasound frequency. Lipid levels increased with an increase in ultrasound frequency up to 30 kHz and then decreased at treatments > 30 kHz. Hemocytes, the fat body, and epidermis expressed three heat shock proteins and apolipophorin III. Ultrasound treatment markedly inhibited expression of some stress-related genes. Ultrasound treatment also inhibited S. exigua pupal development by extending the pupal developmental period and preventing adult emergence. Last, ultrasound treatment significantly inhibited adult mating behavior, which resulted in a significant decrease in female fecundity. These results show that ultrasound is a physiological stress to S. exigua.
Shelf-life of functional beverage for the purpose of body fat reduction during storage was estimated at different temperatures. To estimate quality change of functional beverage, vacuum gauge, sugar concentration pit acidify, Hunter's color value, browning color intensity, total cell count and sensory evaluation were measured periodically. Vacuum gauge, sugar concentration pH and acidity were little changed and total cell count was not detected. By using the correlation coefficient between sensory evaluation and physicochemical properties, b-value was chosen for quality index of functional beverage. $Q_{10}-values$ for shelf-life were in the range of $2.13{\sim}2.59$. When sensory evaluation was 2.5 at $50^{\circ}C$, storage period was 6.83 weeks. And shelf-life calculated by $Q_{10}-values$ were 73.89, 34.21 and 13.21 weeks in $20^{\circ}C,\;30^{\circ}C\;and\;40^{\circ}C$, respectively.
Currently, there is a growing interest among consumers in selecting healthier meat with a greater proportion of essential fatty acids (FA). This experiment was conducted to evaluate the role of different ratios of dietary n-6:n-3 on growth performance, FA profile of longissimus dorsi (LD), relative gene expression of cytokines, meat quality, and blood parameters in finishing pigs. A total of 108 finishing pigs was randomly allotted to three treatments including a control (basal diet) and low ratios (4:1 and 2:1) of n-6:n-3. The 4:1 and 2:1 diets decreased the overall stearic acid in LD. There were reductions in the content of stearic acid, palmitoleic acid, total saturated acid, and n-6:n-3 ratio of LD in pigs fed 4:1 and 2:1 diet compared with the control diet. The 4:1 and 2:1 diets increased the concentration of α-Linolenic acid and polyunsaturated FA in the LD of pigs. Acetyl-CoA carboxylase enzyme gene was down-regulated in pigs fed 2:1 diet compared with finishing pigs fed the control or 4:1 diets. The relative expression of hormone-sensitive lipase was increased in pigs fed 2:1 and 4:1 ratio diets. Lower total cholesterol of plasma was observed in finishing pigs fed 2:1 and 4:1 diets. The cooking loss ratio of meat was lower in pigs fed the 2:1 and 4:1 diets compared with the control diet. Pigs fed the 4:1 and 2:1 diets had greater final body weight. In conclusion, the 2:1 and 4:1 diets have the potential to increase the meat quality and growth performance of pigs.
Journal of Korean Home Economics Education Association
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v.28
no.2
/
pp.41-50
/
2016
Fast foods are very popular to adolescent and the sales are increasing due to the convenience, low price, and a broad scale advertisement through mass media. Fast foods are often named as junk foods because of high calorie and fat, low vitamins and minerals contents. This study investigated the intake frequency of hamburger, pizza and fried chicken, the 3 representative fast foods, in 306 male and female high school students living in Daejeon. Data were collected by food frequency questionnaire, and analyzed by SPSS(ver. 18.0). Intake frequency of hamburger, pizza and fried chicken were 33, 29, 17 times per year, respectively. Intake frequency of these 3 fast foods were not sig. different by BMI obesity, breakfast and supper meal frequency, and first time of fast foods intake. But intake frequency of fast foods were different by the allowance, intake frequence of pizza and fried chicken are higher in students with higher allowance. Restrain score of fast foods intake were higher in underweight and obese than normal weight students. There were no sig. difference of intake restrain score by preferable future body figure. As conclusion, the most effective way of reducing the fast food intake frequency in adolescent were cutting down the allowance. Besides that education based on analysis about what are the specific nutritional defects in each fast food may help to change the behavior of fast foods intake.
Thirty-six yearling (18 bulls, 18 heifers) swamp buffaloes (Bubalus bubalis) with average liveweight $177{\pm}26kg$, were randomly allotted to receive 6 dietary treatments according to a $2{\times}3$ factorial arrangement in a completely randomized design (CRD). Factor A assigned for two types of straw; untreated rice straw (RS) and urea-treated (5%, w/w) rice straw (UTS). Factor B assigned for three ratio of roughage to concentrate (R:C) at 80:20, 50:50 and 20:80. Feeding trial lasted for 9 months during which respective feeds were offered at 3% body weight and adjusted at bi-weekly intervals using corresponding liveweights. Parameters measured under this experiment were feed intake, ruminal fluid for pH, $NH_3-N$, volatile fatty acids (VFA), liveweight change at bi-weekly intervals, carcass characteristics and cost-net profit analysis. It was found that intakes and digestion coefficients of DM, OM, CP except NDF and ADF were improved appreciably when ratio of concentrate increased. The average daily gain (ADG) and feed conversion ratio (FCR) were highest in group fed urea-treated rice straw at R:C levels of 20:80 (551.2 g/d, 10.7 kg/kg) and 50:50 (542.3 g/d, 10.6 kg/kg). It was obvious that FCR was best in the group fed on urea-treated rice straw (13.8 kg/kg) as compared to untreated rice straw fed-group (24 kg/kg). Carcass compositions of buffaloes measured resulted in 48.2 dressing percentage in all treatments offered at R:C levels of 50:50 and 20:80, however, loin eye area were 46.0, 53.6, 50.0 and $54.0cm^2$ for RS and UTS at respective levels of R:C. It was notable that carcass fat content was low which resulted in higher content of lean meat particularly in group fed UTS at 50:50 ratio R:C. Simple cost-net profit analysis was performed, it was found that net profits were obtained as follows 27, 30, -47, 44, 58, 22 $US/hd for respective treatment groups of RS and UTS at respective R:C levels. As shown, the best net profit resulted in group fed UTS at 50:50 R:C level.
This study was carried out to establish an optimum number of phase feeding regimen which enable to reduce nutrients excretion without affecting growth performance and to investigate the effects of different feeding regimens on growth performance, nutrients excretion and carcass characteristics in finishing pigs. A total of 120 finishing pigs (an average initial body weight of 54.3 kg) were assigned to the feeding trial and 12 pigs were assigned to the metabolic trial. Treatments included one phase (54 to 104 kg), two phase (54 to 80 and 80 to 104 kg), three phase (54 to 70, 70 to 90 and 90 to 104 kg) and four phase (54 to 65, 65 to 80, 80 to 95, 95 to 104 kg) feeding regimens. Experimental diets were formulated to contain 16% crude protein for one phase feeding regimen, 16% and 12% crude protein for two phase feeding regimen, 16%, 14% and 12% crude protein for three phase feeding regimen, and 16%, 14.7%, 13.4% and 12% crude protein for four phase feeding regimen, respectively. Although there were no significant differences in any criteria measured during the entire experimental period, pigs reared in three phase feeding regimen grew slightly faster than those reared in other feeding regimens and showed a tendency to increase ADFI during the whole experimental period. The metabolic trial indicated that there were no significant differences in DM (dry matter), CP (crude protein) and P (phosphorus) digestibilities. However, fecal nutrient excretion except P was significantly influenced by feeding regimens. DM excretion of one phase feeding group was significantly higher than that of three phase feeding group and daily fecal N (nitrogen) excretion of one phase feeding group was higher than that of other phase feeding groups (p<0.05). Three and four phase feeding regimens resulted in 12% lower fecal N and DM excretion than one phase feeding regimen. Blood urea concentrations were lower for pigs reared in two, three and four phase feeding regimens than for those reared in one phase feeding regimen (p<0.05). Three phase feeding regimen for the finishing period showed better carcass grade than one phase feeding regimen, though the difference was not significant. The tenth rib fat thickness of pigs fed on four phase feeding regimen was reduced most and there was a trend that backfat thickness decreased as the number of phases increased. Feed cost per kg weight gain was significantly low in four phase feeding group than one phase feeding group (p<0.05). In summary, it seemed that producers generally oversupply the expensive nutrients for the finishing pigs. High nutrient diets do not always guarantee high growth rate of pigs and cause more unwanted nutrient excretion. It rather seems that meeting nutrient requirements for the each growth phase is more important for the reduction of pollutants and economical pork production.
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