• 제목/요약/키워드: Fast ion

검색결과 321건 처리시간 0.023초

Purification and Characterization of Extracellular Aspartic Proteinase of Candida albicans

  • Na, Byoung-Kuk;Lee, Seong-Il;Kim, Sin-Ok;Park, Young-Kil;Bai, Gill-Han;Kim, Sang-Jae;Song, Chul-Yong
    • Journal of Microbiology
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    • 제35권2호
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    • pp.109-116
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    • 1997
  • An extracellular proteinase of Candida albicans was purified by a combination of 0~75% ammonium sulfate precipitation, DEAE Sepharose Fast Flow ion exchange chromatography, and Sephacryl S-200 HR molecular sieve chromatography. Its mlecular weight was approximately 41 kDa on SDS-PAGE and isoelectric point was 4.4. The enzyme was inhibited by pepstain A. Optimum enzyme activity ranged from pH 2.0 to 3.5 with its maximum at pH 2.5 and a temperature of 45$^{\circ}C$. The addition of divalent cations, $Ca^{2+}$, Zn$^{2+}$ and $Mg^{2+}$, resulted in no significant inhibition of enzymatic activity. However, some inhibitory effects were observed by Fe$^{2+}$, Ag$^{2+}$ and Cu$^{2+}$. With BSA as substrate, an apparent $K_m$ was determined to be 7$\times$10$^{-7}$ M and $K_i$, using pepstatin A as an inhibitor, was 8.05$\times$10$^{-8}$ M. N-terminal amino acid sequence was QAVPVTLXNEQ. Degradation of BSA and fibronectin was shown but not collagen, hemoglobin, immunoglobulin G, or lysozyme. The enzyme preferred peptides with Glu and Leu at the P$_1$ position, but the enzyme activity was highly reduced when the P$_2$ position was phe or pro. This enzyme showed antigenicity against sera of patients with candidiasis.

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Selective adsorption of Cs+ by MXene (Ti3C2Tx) from model low-level radioactive wastewater

  • Jun, Byung-Moon;Jang, Min;Park, Chang Min;Han, Jonghun;Yoon, Yeomin
    • Nuclear Engineering and Technology
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    • 제52권6호
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    • pp.1201-1207
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    • 2020
  • This study explored whether MXene (Ti3C2Tx) could remove radioactive Cs+ from model nuclear wastewater. Various adsorption tests were performed and the physical aspects of the interaction were investigated. We varied the MXene dosage, Cs+ initial concentration, solution pH, solution temperature and exposure time. MXene adsorption exhibited very fast kinetics, based on the fact that equilibrium was achieved within 1 h. MXene exhibited an outstanding adsorption capacity (148 mg g-1) at adsorbent and adsorbate concentrations of 5 and 2 mg L-1, respectively, at neutral pH condition (i.e., pH 7). We explored Cs+ adsorption by MXene in the presence of four different ions (NaCl, KCl, CaCl2 and MgCl2) and three different organic acids (sodium oleate, oxalic acid, and citric acid). The Cs+ removal rate changed in the presence of these components; adsorption of Cs+ by MXene thus involved ion exchange, supported by both Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. We confirmed that MXene was re-usable for at least four cycles. MXene is cost-effective and practical when used to adsorb radionuclides (e.g., Cs+) in nuclear wastewater.

A Study on the Electrode Characteristics of Hypo-Stoichiometric Zr-based Hydrogen Storage Alloys

  • Lee, Sang-Min;Kim, Seoung-Hoe;Lee, Jai-Young
    • 한국수소및신에너지학회논문집
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    • 제10권4호
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    • pp.197-210
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    • 1999
  • The hydrogen storage performance and electrochemical properties of $Zr_{1-X}Ti_X(Mn_{0.2}V_{0.2}Ni_{0.6})_{1.8}$(X=0.0, 0.2, 0.4, 0.6) alloys are investigated. The relationship between discharge performance and alloy characteristics such as P-C-T characteristics and crystallographic parameters is also discussed. All of these alloys are found to have mainly a C14-type Laves phase structure by X-ray diffraction analysis. As the mole fraction of Ti in the alloy increases, the reversible hydrogen storage capacity decreases while the equilibrium hydrogen pressure of alloy increases. Furthermore, the discharge capacity shows a maxima behavior and the rate-capability is increased, but the cycling durability is rapidly degraded with increasing Ti content in the alloy. In order to analyze the above phenomena, the phase distribution, surface composition, and dissolution amount of alloy constituting elements are examined by S.E.M., A.E.S. and I.C.P. respectively. The decrease of secondary phase amount with increasing Ti content in the alloy explains that the micro-galvanic corrosion by multiphase formation is little related with the degradation of the alloys. The analysis of surface composition shows that the rapid degradation of Ti-substituted Zr base alloy electrode is due to the growth of oxygen penetration layer. After comparing the radii of atoms and ions in the electrolyte, it is clear that the electrode surface becomes more porous, and that is the source of growth of oxygen penetration layer while accelerating the dissolution of alloy constituting elements with increasing Ti content. Consequently, the rapid degradation (fast growth of the oxygen-penetrated layer) with increasing Ti substitution in Zr-based alloy is ascribed to the formation of porous surface oxide through which the oxygen atom and hydroxyl ion with relatively large radius can easily transport into the electrode surface.

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고인성내화모르터(FR-ECC)를 사용한 장대터널 내화안전대책에 관한 연구 (A study on the fire resistance method using FR-ECC in long tunnel)

  • 김세종;김동준;권영진
    • 한국터널지하공간학회 논문집
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    • 제13권1호
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    • pp.9-18
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    • 2011
  • 최근 이동거리와 물류수송의 급격한 증가로 인해 거리의 단축과 지형의 한계를 극복하기위해 장대터널의 수요가 급격히 증대되고 있으며 이에 따라 터널내화재사고에 대한 우려가 함께 증가하고 있다. 특히 터널 화재 온도는 일반 화재 온도와 달리 초기에 급격히 증가하는 현상을 보이고 있다. 하지만 국내에서는 이에 대한 대책마련이 미흡하고 실제시공한 사례가 없으므로 일본의 히다터널에 사용된 고인성 내화모르터의 사용과 국내에서 개발한 신기술의 현장적용을 통해 시공한 사례와 함께 그에 따른 앞으로의 터널구조물의 내화 및 내구성에 대한 방안을 제시해 보았다.

스마트 홈에서 연소에 따른 화재감지기 원격 IoT 작동 시간에 관한 연구 (A Study on Remote IoT operating time for Fire Detector of Smart Home)

  • 고은영;홍성호;차재상
    • 한국인터넷방송통신학회논문지
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    • 제20권2호
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    • pp.235-238
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    • 2020
  • 스마트홈 시대에 화재안전은 인명 및 시설안전에 매우 중요한 사항이다. 화재로 인한 사상자 및 재산상 피해는 국가적으로 큰 손실일 것이다. 본 논문에서는 스마트홈에서 화재에 따라 화재감지기 작동시간을 판단하여 위험성을 예측할 수 있도록 제안하고자 한다. IoT 화재감지기인 열감지기와 연기감지기 중에서 화재에 따라 작동하는 시간의 차이로 인하여 위험성을 예측을 할 수 있다. 본 실험결과를 바탕으로 이온식 연기감지기가 매우 빠른 특성을 보여 추후 화재예방시설시에 본 결과물이 잘 활용되면 좋을 것이다.

1-Aza-15-Crown-5-Styrene (위험물)-DVB 수지에 의한 희토류 금속 이온들의 흡착 특성 (Adsorption Characteristic of Rare Earth Metal Ions on 1-Aza-15-Crown-5-Styrene (Hazardous Materials)-DVB Resin)

  • 노기환;김관천;김선화;김준태
    • 공업화학
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    • 제20권1호
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    • pp.21-27
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    • 2009
  • 1%, 2%, 5% 및 20%의 가교도를 가진 스타이렌(제4류 위험물) 디비닐벤젠 공중합체에 1-aza-15-crown-5 거대고리 리간드를 치환반응으로 결합시켜 수지를 합성하였으며, 이들 수지의 특성은 염소 함량, 원소 분석, 열 중량 분석, 비표면적(BET), 그리고 적외선 분광법으로 확인하였다. 수지 흡착제에 대한 금속 이온의 흡착에 미치는 pH, 시간, 수지의 가교도 그리고 용매의 유전상수에 따른 영향들을 조사한 결과 금속 이온들은 pH 3 이상에서 큰 흡착율을 보였으며, 금속 이온들의 흡착 평형은 2 h 정도였다. 한편, 에탄올 용매에서 수지에 대한 흡착 선택성은 우라늄($UO_2^{2+}$) > 망간 ($Mn^{2+}$) > 프라세듐($Pr^{3+}$) 이온이었고, 금속 이온의 흡착력은 1%, 2%, 5% 및 20%의 가교도 순이며, 용매의 유전상수 크기에 반비례하였다.

농업용 드론의 배터리 셀 밸런싱을 위한 퍼지제어기 개발 (Development of Fuzzy controller for battery cell balancing of agricultural drones)

  • 이상현
    • 한국인터넷방송통신학회논문지
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    • 제17권5호
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    • pp.199-208
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    • 2017
  • 리튬 폴리머 배터리는 높은 안전성, 빠른 충전 및 긴 라이프 사이클 등으로 인해 에너지 저장치(ESS: Energy Storage System), 전기자동차(EVs: Electric Vehicles)등에 채택이 되어 사용되고 있으며, 그리고 현재는 농업용 드론에서 까지 사용이 되고 있다. 그러나 리튬 폴리머 배터리는 과충 방전에는 리튬-이온 배터리 내의 격차구조가 파괴되어 배터리 수명이 줄어들게 되며, 과충 방전을 방지하기 위해 불균등한 셀 전압을 균등 제어 할 수 있는 셀 밸런싱 시스템이 필수적이다. 본 논문은 각 셀의 충 방전할때의 전압차이를 검출하여 불균형된 셀을 확인하여 비선형 시스템에 적합한 퍼지 제어기를 개발하여 적용한 셀별 밸런싱 알고리즘을 제안한다. 본 논문은 농업용 드론의 배터리팩의 셀 밸런싱을 퍼지제어를 하여 셀 간 균등 제어를 위해 설계하였으며, 최종 결과로 셀 간 밸런싱이 잘 되는지 확인하고 자 셀이 2개 있을 때와 6개 그리고 최종적으로 12개의 각 셀 밸런싱이 되는지를 확인하였다. 이는 다른 제품에도 사용할 수 있는지를 실험하고자 하였으며, 확인결과 사용된 셀의 개수와는 관계없이 셀별 밸런싱이 잘 되고 있음을 확인하였다.

A Solid-Contact Indium(III) Sensor based on a Thiosulfinate Ionophore Derived from Omeprazole

  • Abbas, Mohammad Nooredeen;Amer, Hend Samy
    • Bulletin of the Korean Chemical Society
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    • 제34권4호
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    • pp.1153-1159
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    • 2013
  • A novel solid-contact indium(III)-selective sensor based on bis-(1H-benzimidazole-5-methoxy-2-[(4-methoxy-3, 5-dimethyl-1-pyridinyl) 2-methyl]) thiosulfinate, known as an omeprazole dimer (OD) and a neutral ionophore, was constructed, and its performance characteristics were evaluated. The sensor was prepared by applying a membrane cocktail containing the ionophore to a graphite rod pre-coated with polyethylene dioxythiophene (PEDOT) conducting polymer as the ion-to-electron transducer. The membrane contained 3.6% OD, 2.3% oleic acid (OA) and 62% dioctyl phthalate (DOP) as the solvent mediator in PVC and produced a good potentiometric response to indium(III) ions with a Nernstian slope of 19.09 mV/decade. The constructed sensor possessed a linear concentration range from $3{\times}10^{-7}$ to $1{\times}10^{-2}$ M and a lower detection limit (LDL) of $1{\times}10^{-7}$ M indium(III) over a pH range of 4.0-7.0. It also displayed a fast response time and good selectivity for indium(III) over several other ions. The sensor can be used for longer than three months without any considerable divergence in potential. The sensor was utilized for direct and flow injection potentiometric (FIP) determination of indium(III) in alloys. The parameters that control the flow injection method were optimized. Indium(III) was quantitatively recovered, and the results agreed with those obtained using atomic absorption spectrophotometry, as confirmed by the f and t values. The sensor was also utilized as an indicator electrode for the potentiometric titration of fluoride in the presence of chloride, bromide, iodide and thiocyanate ions using indium(III) nitrate as the titrant.

연료전지용 술폰화 폴리아릴렌에테르술폰 랜덤공중합체 강화복합막의 제조 및 특성 (Preparation and Characterization of Sulfonated Poly(Arylene Ether Sulfone) Random Copolymer Reinforced Membranes for Fuel Cells)

  • 안주희;이창현
    • 멤브레인
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    • 제26권2호
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    • pp.146-151
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    • 2016
  • 술폰화 폴리아릴렌에테르술폰(SPAES) 랜덤 공중합체는 고분자 전해질 연료전지에 적용될 때 높은 수소이온전도도, 상대적으로 낮은 생산 단가 그리고 열화학적 저항성등과 같은 장점을 갖는다. 반면, SPAES 공중합체는 가혹한 구동 조건하에서 낮은 화학적 안정성과 치수 불안전성으로 인해 실제 연료전지 막에 직접적으로 적용하는데 어려움이 있다. 그에 타당한 해결책은 SPAES 공중합체를 상호 연결된 기공 구조와 높은 열화학적 강도를 가지는 지지체 필름(예 : 전기방사된 폴리이미드 지지체)에 함침시키는 것이다. 본 연구에서는 함침막 제조를 위한 이오노머로 빠른 이온 수송을 위해 높은 자유 체적을 유도하는 회전 그룹을 갖는 SPAES 공중합체를 선택하였다. 제작된 막의 실용가능성은 막 특성화를 통해 평가되었다.

Lithspermic acid-A slows down the inactivation kinetics of cardiac $Na^+$ current by intracellular $Ca^{2+}$-dependent mechanisms

  • Yoon, Jin-Young;Hyuncheol Oh;Ho, Won-Kyung;Lee, Suk-Ho
    • 한국생물물리학회:학술대회논문집
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    • 한국생물물리학회 2003년도 정기총회 및 학술발표회
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    • pp.46-46
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    • 2003
  • Salviae Miltiorrhizae Radix has been used for treatment of cardiovascular diseases in oriental medicine. To investigate the possible involvement of cardiac ion channel in this effect, we examined electrophysiological effects of the extract of Salviae Miltiorrhizae Radix on action potentials and ionic currents in rat ventricular myocytes. The extracts of Salviae Miltiorrhizae Radix were fractionated into nine fractions, and the effect of each fraction on action potential was tested. The fraction containing monomethyl lithospermic acid-A (LSA-A) induced a significant prolongation of action potential duration (APD). LSA-B which is a major component of Salviae Miltiorrhizae Radix, however, did not cause a significant effect. In voltage clamp experiments, the effects of LSA-A on K currents, Ca currents and Na currents were tested. Neither K currents nor L-type Ca currents were affected by LSA-A. On the contrary, LSA-A significantly slowed down the inactivation kinetics of the Na current with no effect on the fast component of the inactivation process. The amplitude of the peak current and the voltage-dependence of activation were not changed by LSA-A. The effect of LSA-A on Na current was abolished when high concentration of $Ca^{2+}$ buffer (10 mM BAPTA) was included in the pipette solution or when Ca2+ current was blocked by nicardipine (1 $\mu$M) in the bath solution.n.

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