• Mycobiology
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• v.47 no.3
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• pp.319-328
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• 2019

Cytospora is a genus including important phytopathogens causing severe dieback and canker diseases distributed worldwide with a wide host range. However, identification of Cytospora species is difficult since the currently available DNA sequence data are insufficient. Aside the limited availability of ex-type sequence data, most of the genetic work is only based on the ITS region DNA marker which lacks the resolution to delineate to the species level in Cytospora. In this study, three fresh strains were isolated from the symptomatic branches of Elaeagnus angustifolia in Xinjiang Uygur Autonomous Region, China. Morphological observation and multi-locus phylogenetic analyses (ITS, LSU, ACT and RPB2) support these specimens are best accommodated as a distinct novel species of Cytospora. Cytospora elaeagnicola sp. nov. is introduced, having discoid, nearly flat, pycnidial conidiomata with hyaline, allantoid conidia, and differs from its relatives genetically and by host association.

• Journal of the Korean Society of Visualization
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• v.17 no.1
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• pp.43-52
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• 2019

'Ionic wind' is phenomenon induced by corona discharge which occurs when large electric potential is applied to electrodes with high curvature. The ionic wind has advantage that it could generate forced convective flow without any external energy like separate pump. In this study, 'pin-mesh' arrangement is utilized for experiments. First, optimization of configuration is conducted with local momentum of ionic wind behind the mesh. Empirical equation for prediction about velocity profile was derived using the measured results. Secondly, the enhancement of mass transfer rate of acetone with ionic wind was analyzed. Also, the drying efficiency using a fan which has same flow rate was compared with ionic wind for identification of additional chemical reaction. At last, the drying process of organic solvent was visualized with image processing. As a result, it was shown that the use of ionic wind could dry organic matter four times faster than the natural condition.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7295-7300
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• 2013

Lung cancer is the most common cause of cancer-related death in the world. The main types are small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC), the latter including squamous cell carcinoma (SCC), adenocarcinoma and large cell carcinoma. NSCLCs account for about 80% of all lung cancer cases. Microcephalin (MCPH1), also called BRIT1 (BRCT-repeat inhibitor of hTERT expression), plays an important role in the maintenance of genomic stability. Recently, several studies have provided evidence that the expression of MCPH1 gene is decreased in several different types of human cancers. We evaluated the expression of protein MCPH1 in 188 lung cancer and 20 normal lung tissues by immunohistochemistry. Positive MCPH1 staining was found in all normal lung samples and only some cancerous tissues. MCPH1-positive cells were significantly lower in lung carcinoma compared with normal tissues. Furthermore, we firstly found that MCPH1 expression in lung adenocarcinoma is higher than its expression in squamous cell carcinoma. Change in MCPH1 protein expression may be associated with lung tumorigenesis and may be a useful biomarker for identification of pathological types of lung cancer.

• Structural Engineering and Mechanics
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• v.26 no.6
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• pp.709-723
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• 2007

Considering the vast usage of time-history dynamic analyses to calculate structural responses and lack of sufficient and suitable earthquake records, generation of artificial accelerograms is very necessary. The main target of this paper is to present a novel method based on nonstationary Kanai-Tajimi model and wavelet transform to generate more artificial earthquake records, which are compatible with target spectrum. In this regard, the generalized nonstationary Kanai-Tajimi model to include the nonstationary evaluation of amplitude and dominant frequency of ground motion and properties of wavelet transform is used to generate ground acceleration time history. Application of the method for El Centro 1940 earthquake and two Iranian earthquakes (Tabas 1978 and Manjil 1990) is presented. It is shown that the model and identification algorithms are able to accurately capture the nonstationary features of these earthquake accelerograms. The statistical characteristics of the spectral response of the generated accelerograms are compared with those for the actual records to demonstrate the effectiveness of the method. Also, for comparison of the presented method with other methods, the response spectra of the synthetic accelerograms compared with the models of Fan and Ahmadi (1990) and Rofooei et al. (2001) and it is shown that the response spectra of the synthetic accelerograms with the method of this paper are close to those of actual earthquakes.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.767-773
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• 2012

The esophageal squamous cell carcinoma (ESCC) is an aggressive tumor with a poor prognosis. Understanding molecular changes in ESCC should improve identification of risk factors with different molecular subtypes and provide potential targets for early detection and therapy. Our study aimed to obtain a molecular signature of ESCC through the regulation network based on differentially expressed genes (DEGs). We used the GSE23400 series to identify potential genes related to ESCC. Based on bioinformatics we constructed a regulation network. From the results, we could establish that many transcription factors and pathways closely related with ESCC were linked by our method. STAT1 also arose as a hub node in our transcriptome network, along with some transcription factors like CCNB1, TAP1, RARG and IFITM1 proven to be related with ESCC by previous studies. In conclusion, our regulation network provided information on important genes which might be useful in investigating the complex interacting mechanisms underlying the disease.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.761-766
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• 2012

Objective: Although various human cancer stem cells (CSCs) have been defined, their applications are restricted to immunocompromised models. Developing a novel CSC model which could be used in immunocompetent or transgenic mice is essential for further understanding of the biomolecular characteristics of tumor stem cells. Therefore, in this study, we analyzed murine lung cancer cells for the presence of CSCs. Methods: Side population (SP) cells were isolated by fluorescence activated cell sorting, followed by serum-free medium (SFM) culture, using Lewis lung carcinoma cell (LLC) line. The self-renewal, differentiated progeny, chemosensitivity, and tumorigenic properties in SP and non-SP cells were investigated through in vitro culture and in vivo serial transplantation. Differential expression profiles of stem cell markers were examined by RT-PCR. Results: The SP cell fraction comprised 1.1% of the total LLC population. SP cells were available to grow in SFM, and had significantly enhanced capacity for cell proliferation and colony formation. They were also more resistant to cisplatin in comparison to non-SP cells, and displayed increased tumorigenic ability. Moreover, SP cells showed higher mRNA expression of Oct-4, ABCG2, and CD44. Conclusion: We identified SP cells from a murine lung carcinoma, which possess well-known characteristics of CSCs. Our study established a useful model that should allow investigation of the biological features and pharmacosensitivity of lung CSCs, both in vitro and in syngeneic immunocompetent or transgenic/knockout mice.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.10
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• pp.77-84
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• 1998

The temperature difference occurs at the test points of chamber, because the traditional Test Handler consists of a heater and a fan. To reduce the temperature variation at different points in the chamber, we divided the heater to three parts which are controlled independently. First, we identified the chamber's parameter and then designed a LQG controllers by the identification model. RTD sensors and VME system were used to construct the temperature control system. In our experiment for the proposed control system, the temperature variation was reduced from ${\pm}1.5^{\circ}C$ to ${\pm}0.35^{\circ}C$ at 50$^{\circ}C$ ${\cdot}$ 80$^{\circ}C$ and 120$^{\circ}C$.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.03a
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• pp.369-375
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• 2004

Gas turbine engine simulation in terms of transient, steady state performance and operational characteristics is complex work at the various engineering functions of aero engine manufacturers. Especially, efficiency of control system design and development in terms of cost, development period and technical relevance implies controlling diverse simulation and identification activities. The previous engine simulation has been accomplished within a limited analysis area such as fan, compressor, combustor, turbine, controller, etc. and this has resulted in improper engine performance and control characteristics because of limited interaction between analysis areas. In this paper, we propose a new simulation methodology for gas turbine engine performance analysis as well as its digital controller to solve difficulties as mentioned above. The novel method has particularities of (ⅰ) resulting in the integrated control simulation using almost every component/module analysis, (ⅱ) providing automated math model generation process of engine itself, various engine subsystems and control compensators/regulators, (ⅲ) presenting total sophisticated output results and easy understandable graphic display for a final user. We call this simulation system GT3GS (Gas Turbine 3D Graphic Simulator). GT3GS was built on both software and hardware technology for total simulation capable of high calculation flexibility as well as interface with real engine controller. All components in the simulator were implemented using COTS (Commercial Off the Shelf) modules. In addition, described here includes GT3GS main features and future works for better gas turbine engine simulation.

• Korean Journal of Ichthyology
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• v.36 no.1
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• pp.101-106
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• 2024

In May 2020, a single larval specimen (5.14 mm in total length) was collected from the southeastern sea of Jeju Island of Korea using bongo net. The specimen was identified as Lepidotrigla longifaciata based on mitochondrial DNA cytochrome c oxidase subunit I sequences. The morphological traits of the L. longifaciata larva are as follows: a long snout, a large mouth, large fan-shaped pectoral fins, and black melanophores scattered on the abdominal cavity and nape. We propose the new Korean name 'Gin-meo-ri-dal-jae' for this species, which was first discovered in Korea.

• BMB Reports
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• v.43 no.5
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• pp.355-362
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• 2010

The sterile alpha motif (SAM) is a putative protein interaction domain involved in a wide variety of biological processes. Here we report the identification and characterization of a novel gene, SAMD4B, which encodes a putative protein of 694 amino acids with a SAM domain. Northern blot and RT-PCR analysis showed that SAMD4B is widely expressed in human embryonic and adult tissues. Transcriptional activity assays show SAMD4B suppresses transcriptional activity of L8G5-luciferase. Over-expression of SAMD4B in mammalian cells inhibited the transcriptional activities of activator protein-1 (AP-1), p53 and p21, and the inhibitory effects can be relieved by siRNA. Deletion analysis indicates that the SAM domain is the main region for transcriptional suppression. The results suggest that SAMD4B is a widely expressed gene involved in AP-1-, p53- and p21-mediated transcriptional signaling activity.