• Applied Microscopy
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• 제32권4호
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• pp.411-419
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• 2002

사람의 위암 조직을 미세구조와 metallothionein (MT)에 대한 면역 조직 및 세포화학적 방법으로 조사하였던 바, 다음과 같은 결과를 얻어냈다. 위암 세포들은 핵 세포질비가 정상세포에 비해 크고, 불규칙한 핵과 이질염색질의 분포가 증가하였으며, 세포질내에서 유리리보솜의 분포가 뚜렷이 증가하였다. 면역 조직 및 세포화학적 방법으로 MT의 발현을 조사하였던 결과, 이 단백질은 위암조직의 암세포에서 반응성이 높게 나타났으며, 주로 핵 부위에 집중되는 경향을 보였으며, 특히 이질염색질과 인 부위에서 면역 금입자들이 주로 분포하는 것으로 관찰되었다. 이러한 결과들은 위암 세포의 미세구조가 미분화세포들이 나타내는 일반적인 특징과 비교되었으며, 위암 세포에서 MT가 증가하는 현상은 이 단백질이 세포질에서 합성되어 핵내로 수송된 후, 세포 증식을 위한 전사과정에 관여할 것임을 시사하는 것이다.

• Journal of Preventive Medicine and Public Health
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• 제44권3호
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• pp.125-130
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• 2011

Objectives: The microRNA (miRNA) miR-196a2 may play an important role in lung cancer development and survival by altering binding activity of target mRNA. In this study, we evaluated their associations with the susceptibility of non-small cell lung cancers (NSCLC) by case-control study in a Korean population. Methods: We performed genotyping analyses for miR-196a2 rs11614913 T/C at miRNA regions in a case-control study using blood samples of 406 NSCLC patient and 428 cancer-free control groups. Results: The total C allele frequencies for miR-196a2 were 48.8% for the patients and 45.6% for the controls; and the genotype frequencies of TT, TC, and CC were 23.7%, 55.2%, and 21.1% for the patients and 31.1%, 46.35%, and 22.4% for the controls (p<0.05). Participants who possesses TC/CC genotypes showed high risk for NSCLC compared to those possessed TT genotypes (OR, 1.42; 95% CI, 1.03 to 1.96). The association was persisted in 60 and older age group, male, smokers, those without family history for cancer. However, no significant association of CC genotypes in recessive genetic model was observed. Conclusions: In conclusion, this case-control study provides evidence that miR-196a2 rs11614913 C/T polymorphisms are associated with a significantly increased risk of NSCLC in a dominant model, indicating that common genetic polymorphisms in miR-196a2 rs11614913 are associated with NSCLC. The association of miR196a2 rs11614913 polymorphisms and NSCLC risk require confirmation through additional larger studies.

• Journal of Chest Surgery
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• 제17권1호
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• pp.118-124
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• 1984

Congenital Cystic Adenomatiod Malformation (C.C.A.M.) is rare, but one of the most common congenital pulmonary anomalies that cause acute respiratory distress in the newborn infants. It is characterized and differentiated from the diffuse pulmonary cystic disease pathologically, i.e. adenomatoid appearance due to marked proliferation of the terminal respiratory components. An 2/12 year old male patient was suffered from respiratory distress and cyanosis on crying since birth, but no specific therapy was given. With progression of symptoms, he came to Korea University Hospital for further evaluation and then transfered to Dept. of Chest Surgery for operative correction under the impression of Congenital Obstructive Emphysema suggested by a pediatrician. On gestational and family history, there was nothing to be concerned such as congenital anomaly. Physical examinations showed; moderate nourishment and development (Wt. 5.5kg), cyanosis on crying, both intercostal and lower sternal retraction on inspiration, Lt. chest building with tympany, Rt. shifting of cardiac dullness, decreased breathing sound with expiratory wheezing on entire Lt. lung field, decreased breathing sound on Rt. upper lung filed, and tachycardia. The remainders were nonspecific. Laboratory findings were normal except WBC $14000/mm^3$ (lymphocyte 70%), Hgb 9.8m%, Hct 28%, negative Mantaux test, and sinus tachycardia and counter-clockwise rotation on EKG. Preoperative simple Chest PA revealed marked hyperlucent entire Lt. lung, herniation of Lt. upper lobe to Rt., collapsed Rt. upper lobe, tracheal deviation and mediastinal shifting to Rt., and no pleural reaction. At operation, after Lt. posterolateral thoracotomy, 4th rib was resected. Operative findings were severe emphysematous changes limited to both lingular segmentectomy was done. The resected specimen showed slight solidity, measuring $8{\times}4.5{\times}2cm$ in size, and small multiple cystic spaces filled with air. Microscopically, entire tissue structures were glandular in appearance, cyst were lined by ciliated columnar epithelium, and occasional cartilages were noted around the cystic spaces. Bronchial elements were dilated but normal pattern on histologically. The patient had a good postoperative courses clinically and radiologically, and discharged on POD 10th without event. The authors report a case of Cogenital Cystic Adenomatoid Malformation (C.C.A.M.)

• 지역사회간호학회지
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• 제14권2호
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• pp.323-333
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• 2003

This is a comparative descriptive study conducted for 178 middle-aged women who were residing in Seoul and aged between 41 to 64 (95 in control group, and 83 in experimental group). The study was aimed to examine their climacteric symptoms, degree of fatigue, depression, anxiety and quality of sleep and to examine the differences on the symptoms between the two groups. Data were collected from April 1 to May 15, 2002 through self-statement using structured questionnaires. The collected data were verified with descriptive statistics, ? -test, t-test, ANOVA using SPSS/PC(+). The results are as follows. 1) The average scores of the climacteric symptoms were 1.56 .36 for the control group, and 1.55 .33 for the group taking relexzone massage, showing no significant difference between the groups (t=.15, p=.88). 2) The average scores of the degree of fatigue were 2.17 .65 for the control group, and 2.40 .66 for the group taking relexzone massage, showing a significant difference between the groups. (t=-2.31, p=.02) 3) The average scores of depression were 1.91 .50 for the control group, and 2.05 .42 for the group taking relexzone massage, showing a significant difference between the groups (t=-1.99, p=.05). 4) The average scores of anxiety were 1.54 .60 for the control group, and 1.57 .53 for the group taking relexzone massage, showing no significant difference between the groups (t=-.33, p=.74). 5) The average scores of quality of sleep were 2.97 .49 for the control group, and 2.98 .42 for the group taking relexzone massage, showing no significant difference between the groups(t=-.08, p=.93). Based on these results, the middle-aged women who take relexzone massage are considered to have less fatigue and depression than those who do not. As physiological regression progresses and the structure and role in the family are restructured, middle-aged women are like to experience various climacteric symptoms. As a nursing intervention, relexzone massage can be applied to middle-aged women to reduce their stress, climacteric symptoms and emotional anxiety, ultimately promoting their health. In order to implement relexzone massage as a nursing intervention, further testing on the psycho-neuroimmunologjcal effects of relexzone massage is necessary.

• 지역사회간호학회지
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• 제7권2호
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• pp.183-202
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• 1996

This study was designed out to develop a home health care service for nurses working in community care services. This study investigates actual conditions at welfare institutions related to health needs the demands of clients, and the state of home health care services we hope that this study will improve upon the current service system. In Korea home health care services are still developing and only new becoming a part of the health care supply system. The data was collected by recording the client home nursing assessments modified to the situation of UTMB home health agency. In this study 107 clients were selected for home care who needed care for physical and mental deficits. The study lasted from March to November of 1995 at one of the welfare service institutions in Chunchon city. The results show that those who most frequently needed care services were over 50 years old with a health deficit of 80.3%, followed by sex as women who needed care at 59.8%. 50.5% of the clients had very little education. 99.1% of the clients live with their family, and a medical diagnostic analysis reveals that 73.9% of the 5 year period of illnesses were the following : 38.8% - muscular -skeleton system disorder, 24.4% - hypertension and stroke, 25.7% sole disease of arthritis. For behavioral conditions 43.3% of the patients were without care services, 56.6% of the patients were taking treatment that 73.5% of those were taking medication. The most main complaint of patients were 22.4% of pain in the extremities, next were 16.8% of a limitation of body activities, 15.0% was lumbo-sacralgia. According to the investigator who was a senior student nurse, the following suggestions were made: 32.7% for curative medical services, 29.9% for physical exercise, 19.6% for emotional support. Consultation nursing services consisted of 67.2% for physical therapy, 11.2% for the maintaining healing, 9.4% for counseling. The patients at home, required assistance most frequently for muscular-skeleton problems under the category of physical systems (33.3%). But, on the other hand, 49.5% of the patients required care givers at home, 28.2% had a knowledge deficit, 21.0% had malnutrition, 18.4% had bad impaired communication. The character of health problems were devided into chronic disease(67.0%), accidents(I3.1%), and general disease(15.9%). 86% of the disabled client had an impairment of the physical system. Eating (86.9%) , Toileting(77.6%), and personal care showed much the same of ADL condition, the level and range of achievement of mobility, the most frequently self performed was 81.3% only in a room size area, and 40.2% were completely dependent when going out. Although there were a large number of home care services in th community at these welfare institutions, many clients needed a variety of curative services. As policy changes have gathered momentum, responsibility for the development of a more suitable program was demanded by the clients from the community.

• 대한예방한의학회지
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• 제13권3호
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• pp.55-71
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• 2009

Background : Traditional Korean medicine(TKM) has gained in popularity among chronic patients in recent years. The use of TKM among patients with chronic diseases is common, with about two thirds of patients using some form of TKM in Korea. Objectives : The purpose of this study is to analyze the use of TKM and determine what factors affect to use TKM among patients with chronic diseases. The study also aims to provide information on TKM therapies and assist therapy selection among various TKM therapies for patients with chronic diseases. Methods : The design of the study was descriptive cross-sectional, and data were collected using a 21-item questionnaire. This study was conducted with subjects with confirmed diagnosis of chronic diseases, who live in Jeju Province, Korea. Results : Among the participants, past or current TKM use was 66.7%, with a statistically significant difference in gender and level of health status groups(p<0.05), but no difference in age, marital status, education, occupation, and income groups. The most common therapies of TKM used by the patients included acupuncture(51.1%), physiotherapy(16.8%), cupping(13.5%), and herbal medicine(4.8%). The main benefits from TKM perceived by the patients were chronic diseases management and health promotion. In a logistic regression analysis, significant influencing factors related to TKM use were gender, family income level, the extent of recognition of efficacy, heath status, and health security program. Conclusions : In this study the socio-demographic and health status, recognition of TKM efficacy factors associated with TKM use among patients with chronic diseases were similar to those found in the general population. The findings suggest that due to the relatively high use of TKM among patients with chronic diseases in Korea, this topic should be taken into account in the development of a holistic approach for patients with chronic diseases and an efficient chronic disease management system. Additionally proactive and consistent management of TKM is necessary in the health care system in Korea.

• The Korean Journal of Physiology and Pharmacology
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• 제14권3호
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• pp.119-125
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• 2010

We investigated the effects of a hot-water extract of Artemisia iwayomogi, a plant belonging to family Compositae, on cardiac ventricular delayed rectifier $K^+$ current ($I_K$) using the patch clamp technique. The carbohydrate fraction AIP1 dose-dependently increased the heart rate with an apparent $EC_{50}$ value of $56.1{\pm}5.5\;{\mu}g/ml$. Application of AIP1 reduced the action potential duration (APD) in concentration-dependent fashion by activating $I_K$ without significantly altering the resting membrane potential ($IC_{50}$ value of $APD_{50}$: $54.80{\pm}2.24$, $IC_{50}$ value of $APD_{90}$: $57.45{\pm}3.47\;{\mu}g/ml$). Based on the results, all experiments were performed with $50\;{\mu}g/ml$ of AIP1. Pre-treatment with the rapidly activating delayed rectifier $K^+$ current ($I_{Kr}$) inhibitor, E-4031 prolonged APD. However, additional application of AIP1 did not reduce APD. The inhibition of slowly activating delayed rectifier $K^+$ current ($I_{Ks}$) by chromanol 293B did not change the effect of AIP1. AIP1 did not significantly affect coronary arterial tone or ion channels, even at the highest concentration of AIP1. In summary, AIP1 reduces APD by activating $I_{Kr}$ but not $I_{Ks}$. These results suggest that the natural product AIP1 may provide an adjunctive therapy of long QT syndrome.

• 한국산학기술학회논문지
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• 제19권5호
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• pp.489-498
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• 2018

본 연구는 일반인들 가운데에서 죽음을 어떻게 인식하고 있느냐에 따라 삶의 태도나 방식이 달라지기 때문에 행복에 따른 죽음인식에 차이가 있는지 알아보고, 행복지수가 높은 그룹과 행복지수가 낮은 그룹간의 호스피스 관심이 차이가 있는지 연구하고자 한다. 2017년 4월 22일 D 광역시에서 열린 걷기대회 행사에 참가한 20세 이상 성인 남녀 200명을 대상으로 설문조사를 실시하였다. 설문구성은 일반적 특성이 성별, 연령, 학력, 직업, 결혼상태, 가족 동거여부, 종교, 건강상태 8항목이었다. 행복에 관한문항은 총 29항목, 죽음 인식은 9항목으로 구성하였다. 통계 검정법으로는 설문지의 항목에 대하여 행복지수를 기준으로 카이제곱 검정, 독립표본 t-검정, 상관분석, 정준상관분석을 실시하였다. 연구결과, 임종하기 적합한 장소에 관한 문항에서 행복지수가 낮은 그룹은 "의료기관"이 46.3%로 가장 높았으며, 행복지수가 높은 그룹에서는 "살던 집"이 59.4%로 가장 높게 나타나 유의한 차이를 보였다. 병원에서 임종하는 경우가 훨씬 많은 우리나라의 현실에서 환자들이 원하는 편안하고 익숙한 장소인 집에서 임종을 맞이할 수 있는 방법인 가정형 호스피스의 이용률을 높일 수 있도록 전문 인력의 확보 및 교육 등 관리체계를 보완해야 할 것이라고 보여 진다. 그리고 대상자의 일반적 특성을 고려한 개별적이고 다양한 접근을 통하여 죽음인식을 높이고 호스피스를 효과적으로 이용할 수 있도록 하여야 할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2413-2418
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• 2015

Background: Although adjuvant chemotherapy is a standard treatment in stage III colon cancer, its benefit is not as clear for stage II patients. In this retrospective analysis, we aimed to evaluate the survival of patients with low-risk stage II colon cancer, the efficacy of adjuvant chemotherapy in high-risk stage II colon cancer patients, and prognostic factors in stage II disease. Materials and Methods: One hundred and seventeen patients who were diagnosed with stage II colon cancer between January 2006 and December 2011 were included in the study. Patients were stratified into two groups as being low-risk and high-risk according to risk factors for stage II disease. Adjuvant 5-fluorouracil-based chemotherapy were administered to the patients with risk factors. Results: Ninety-four patients were treated with adjuvant chemotherapy due to high risk factors and 23 were monitored without treatment. Median follow-up time was 43 months. In terms of disease free survival and overall survival, adjuvant chemotherapy did not provide a statistically significant difference. Univariate analysis demonstrated that bowel obstruction was the major risk factor for shortened disease-free survival, while bowel perforation and perineural invasion were both negative prognostic factors for overall survival. Conclusions: The recommendation of adjuvant chemotherapy for stage II colon cancer is not clear. In our study, it was found that adjuvant chemotherapy did not contribute to survival in high-risk stage II patients. Due to the fact that prognosis of stage II patients is good, many more patients will be needed for statistically significant differences in survival. Adjuvant chemotherapy containing 5 fluorouracil is being used to high-risk stage II patients although it is not a standard treatment approach.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9655-9660
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• 2014

Background: Nigella Sativa (NS) is an herb from the Ranunculaceae family that exhibits numerous medicinal properties and has been used as important constituent of many complementary and alternative medicines (CAMs). The ability of NS to kill cancer cells such as PC3, HeLa and hepatoma cells is well established. However, our understanding of the mode of death caused by NS remains nebulous. The objective of this study was to gain further insight into the mode and mechanism of death caused by NS in breast cancer MCF-7 cells. Materials and Methods: Human breast cancer cells (MCF-7) were treated with a methanolic extract of NS, and a dose- and time-dependent study was performed. The $IC_{50}$ was calculated using a Cell Titer $Blue^{(R)}$ viability assay assay, and evidence for DNA fragmentation was obtained by fluorescence microscopy TUNEL assay. Gene expression was also profiled for a number of apoptosis-related genes (Caspase-3, -8, -9 and p53 genes) through qPCR. Results: The $IC_{50}$ of MCF-7 cells was $62.8{\mu}L/mL$. When MCF-7 cells were exposed to $50{\mu}L/mL$ and $100{\mu}L/mL$ NS for 24h, 48h and 72h, microscopic examination (TUNEL assay) revealed a dose- and time-dependent increase in apoptosis. Similarly, the expression of the Caspase-3, -8, -9 and p53 genes increased significantly according to the dose and time. Conclusions: NS induced apoptosis in MCF-7 cells through both the p53 and caspase pathways. NS could potentially represent an alternative source of medicine for breast cancer therapy.