• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.97.1-97
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• 2003

An antagonistic bacterium, KJ1R5,, to Phytophthora capsici was obtained from root interior of a healthy pepper plant. To identify the bacterial antagonist, 16S rDNA sequence analysis, Biolog system, fatty acid methyl-esters (FAMEs), and physiological and biochemical characterization were conducted. The determined 165 rDNA sequence of KJ1R5, showed higher similarities to those of a group consisting of several Chryseobacterium strains with 95.2, 95.2, and 95,1％ similarity to C. defluvii, Chryseobacterium sp. FR2, and C. scophthalmum, respectively, In addition, Halounella gailinarum, Bergeyella zoohelcum, and Riemerella anatipestifer are another group for KJ1R5, with 94.1, 89.7, and 87.2％ similarities, respectively When identification of the antagonistic bacterium, KJ1R5, was conducted using BIOLOG system, the strain KJ1R5, was identified as Flavobacterium tirrenicum (similarity； 0.75％). Fatty acid profiles of the strain KJ1R5, were composed mainly of iso-17：0 w9c and iso-15：0 and identified as Chryseobacterium balustinum (similarity 0.524％). KJ1R5, was Gram-negative, regular short rods ranging from 0.8 $\mu\textrm{m}$ to 1.0 $\mu\textrm{m}$ and had no flagella. Phenotypic characterization of the antagonistic bacterium indicated that KJ1R5, were included in the genus Chreseobacterium, which belongs to the family Flavobacteriaceae. The strain was distinguished from these six existing species. These results indicated that strain might be placed as a new species in the genus Chryseobacterium.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.18-18
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• 2010

Glucosinolates(GSLs) are major secondary products($\beta$-thioglucoside N-hydroxysulfates) containing sulfates that are found mainly in Cruciferae family such as cabbage, broccoli, radish, turnip, kale, mustard and rapeseed as the important crops in agronomic and economic aspects. Especially, isothiocyanates(ITCs) have been shown to inhibit carcinogenesis with regulation cancer cell development followed by regulating target enzymes, inducing cell cycle arrest and apoptosis against various human cancer cell lines. Total desulfo(DS)-GSLs of nine type in 210 Korean leaf mustard accessions were isolated and confirmed using electrospray ionization mass spectrometric(ESI-MS/MS) method and DS-GSLs powerful library maked from several reference data. The isolated individual DS-GSLs were identified by removed $\beta$-D-glucopyranose residue ($C_6H_{11}O_5$, MW 163) from MS/MS data. Among them sinigrin(41.7%), glucoiberverin(21.7%) and gluconasturtiin(12.6%) were major components, Especially, despite gluconapin was minor component, accession K046197-1 and K046197-2 showed higher content of 4.11 and 3.31 mg/g(DW), respectively. The total GSLs contents in 210 accessions were ranged from 5.3 to 23.2 mg/g(DW) with a mean value of 13.0 mg/g(DW). As a result of principal component analysis(PCA), the individual GSLs loading plots were composed of three groups and components belonged to each group showed correlationship in quantitative pattern.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.160-160
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• 2017

Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic Leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of both OsbZIP66 in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.

• Proceedings of the Korean Society of Food Science and Nutrition Conference
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• 2001.12a
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• pp.38-47
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• 2001

L-theanine is a unique amino acid, found almost solely in tea plants. It is the main component responsible for the exotic taste of green tea. In our studies of L-theanine, we have found a variety of biological activities including relaxation and the alleviation of PMS. In general, animals generate very weak electric pulses on the surface of the brain, called brain waves. Brain waves are classified into four types, namely $\alpha$, $\beta$, $\delta$ and $\theta$-waves, based on their frequency. Brain waves correlate with individual mental conditions. For example, generation of $\alpha$-waves is considered an index of relaxation. In human volunteers, $\alpha$-waves were generated on the occipital and parietal regions of the brain surface within 40 minutes after the oral administration of 50 or 200 mg Suntheanine$^{TM}$ without causing drowsiness. Premenstrual Syndrome (PMS) is a symptom unique to women which appears in the luteal phase from the ovulation period through the first day of menstruation. It possesses characteristics of having a peak just prior to menstruation and disappearing 1 - 2 days following the start of menstruation. Symptoms of PMS are generally categorized as mental, physical and social symptoms. When comparing the reported Symptoms of PMS by the methods of MDQ score, the Suntheanine$^{TM}$ group was found to have a lower incidence of PMS symptoms, including physical, mental and social symptoms. Overall, a significant alleviation of PMS symptoms by the administration of 200 mg Suntheanine$^{TM}$ was observed. With the successful industrial production of L-theanine, we are now able to supply Suntheanine$^{TM}$, offering a tremendous opportunity for designing functional foods targeting relaxation and the alleviation of PMS.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.212-212
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• 2022

Soluble starch synthase (SS) IV-2 is one of the starch synthase gene family members and responsible for starch chain elongation interacting with other rice eating and cooking quality controlling genes (e.g., AGPlar and PUL). SSIV-2 is mainly expressed in leaves, especially at grain-filling stage and its alleles can significantly affect rice quality. Here, we investigated the genetic diversity and population structure analyses of SSIV-2 gene by using 374 rice accessions. This rice set was grouped into 320 cultivated bred (subsequently classified into temperate japonica, indica, tropical japonica, aus, aromatic and admixture) and 54 wild rice. Haplotyping of cultivated rice accessions provided a total of 7 haplotypes, and only three haplotypes are functional indicating four substituted SNPs in two exons of chromosome 5: T/A and G/T in exon 4, and C/G and G/A in exon 13. Including the wild, a highest diverse group (0.0041), nucleotide diversity analysis showed temperate japonica (0.0001) had a lowest diversity value indicating the origin information of this gene evolution. Higher and positive Tajima5s D value of indica (1.9755) indicate a selective signature under balancing selection while temperate japonica (-0.9018) was in lowest Tajima's D value due to a recent selective sweep by positive selection. We found the most diverse genetic components of the wild in PCA but shared in some portion with other cultivated groups. Fixation index (F_ST-values) and phylogenetic analysis indicate a closer relationship of the wild with indica (F_ST=0.256) than to its association to both of temperate japonica (F_ST=0.589). Structure analysis shows a clear separation of cultivated subpopulations at every K value, but genetic components were admixed within the wild illustrating the same genetic background with japonica and indica in some proportion.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.84-88
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• 2007

우리나라의 토양조사는 미농무성(USDA)의 분류방법에 따라 개략토양조사($'65{\sim}'67$년), 정밀토양조사($'68{\sim}'79$년), 논토양배양사업 10개년계획($'80{\sim}'90$년), 그리고 밭토양세부정밀토양조사($'95{\sim}'99$년) 등을 실시하였다. 현지에서의 토양조사는 지형, 배수등급, 토성, 자갈함량, 유효토심, 경사 등 현지의 지형지물 및 환경을 통하여 많은 정보를 얻을 수 있으며, 이를 통하여 전국에 대한 토양조사 결과, 토양통 (심토의 특성이 유사한 토양) 390개, 토양구(표토의 토성을 세분) 536개, 토양상(경사도 및 침식등급을 세분) 1,321개로 분류되어 있다. 우리나라 토양형성 발달에 미친 기후인자는 습윤권역(Udic moisture regime)과 토양온도는 mesic과 일부 남해안과 제주지역의 thermic인 토양온도(Soil moisture regime)였으며, 식생인자는 높은 지역의 경우에는 산림이 낮은 저지대 평탄지는 자연초지가 대부분이다. 지형인자는 동고서저지형으로 동부는 산악지, 서부는 경사가 완만한 구릉지며, 한강 등 대부분이 서남방향으로 흐르는 지형이다. 모재는 다양하나 70%이상이 화강암, 화강편마암이 주를 이루고 있었다. 시간인자의 경우에는 기간이 매우 오래되었으나, 지형이 복잡하여 토양발달이 약한 Inceptisol과 층위발달이 없는 Entisol이 대부분이며, 다음으로 Ultisol과 Alfisol이 뒤를 이었다. 일부 Andisol, Mollisol, Histosol이 존재하였다. 우리나라의 토양분류는 Soil Taxonomy에 의해 분류되었는데, 이것은 토양의 단면내에 존재하는 감식층위(Diagnostic Horizon)의 유무 및 종류에 따른 분류이다. 토양분류 결과 최고 상위분류 단위인 목(order)이 7개, 아목(suborder) 14개, 대군(great group) 27개, 아군(sub group) 67개, 속(family) 161개로 분류되었으며, 가장 저차단위인 통(series)이 390개 분류되었다. 또한 작물이용측면에서 실용적 분류를 실시하였는데, 논토양의 경우 보통논, 미숙논, 사질논, 습논, 염해논, 특이산성논으로 분류할 경우 각각의 분포면적이 31.9%, 23.0%, 31.9%, 9.1%, 3.9%, 0.2%이었다. 밭토양의 경우에도 보통밭, 사질밭, 미숙밭, 중점밭, 고원밭, 화산회밭으로 6개 유형으로 분류할 경우 각각의 분포면적은 41.9%, 23.3%, 17.5%, 13.9%, 1.1. 2.2% 이었다. 도시화 및 도로확대 등 다양한 토지이용 및 지형개변으로 과거의 토양정보가 많이 변경되었다. 그래서, 앞으로는 인공위성자료 및 항공사진을 이용하여 빠르고 쉽게 활용할 수 있는 토양조사 방법개발과 기 구축된 토양도의 수정, 보완 작업이 필요한 절실히 요구되고 있는 현실이다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.708-711
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• 2017

The purpose of this study was to examine the perception of abortion and suicide for 115 paramedic students in 2016. The questionnaire consisted of perception of abortion(8items), the perception of suicide(58items), related 6items of the abortion and related 2 items of the suicide. The collected data were analyzed through t-test, Chi-squared test and ANOVA, using SPSS 21.0. As a result, the total perception score against abortion was $20.57{\pm}4.12$ and the total score of suicide perception was $-14.16{\pm}15.76$. The most answer was that pregnant women were abortion decision makers in both male and female students, and abortions were the highest when they might be unwanted pregnancies. The group who had thought about suicide was higher of perceptions of suicide than the group who did not. The female students who had suicide attempt or suicide experience of close acquaintances showed higher suicide perception score than those who did not. Therefore, A bio-ethics education curriculum should be prepared to establish ethics that respects life for future paramedic students who must make decisions at the boundary between death and life. It is necessary to raise the level of positive suicide perceptions through continuing counseling and education focusing on students who have experienced suicide by family members or acquaintances.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.522-526
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• 2007

Since newly emerged disease, the Severe Acute Respiratory Syndrome (SARS), spread from Asia to North America and Europe rapidly in 2003, many researchers have tried to determine where the virus came from. In the phylogenetic point of view, SARS virus has been known to be one of the genus Coronavirus, but, the overall conservation of SARS virus sequence was not highly similar to that of known coronaviruses. The natural reservoirs of SARS-CoV are not clearly determined, yet. In the present study, the genomic sequences of SARS-CoV were analyzed by bioinformatics techniques such as multiple sequence alignment and phylogenetic analysis methods as well multivariate statistical analysis. All the calculating processes, including calculations of the relative synonymous codon usage (RSCU) and other genomic parameters using 30,305 coding sequences from the two genera, Coronavirus, and Lentivirus, and one family, Orthomyxoviridae, were performed on SMP cluster in KISTI, Supercomputing Center. As a result, SARS_CoV showed very similar RSCU patterns with feline coronavirus on the both axes of the correspondence analysis, and this result showed more agreeable results with serological results for SARS_CoV than that of phylogenetic result itself. In addition, SARS_CoV, human immunodeficiency virus, and influenza A virus commonly showed the very low RSCU differences among each synonymous codon group, and this low RSCU bias might provide some advantages for them to be transmitted from other species into human beings more successfully. Large-scale genomic analysis using bioinformatics techniques may be useful in genetic epidemiology field effectively.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2010.05a
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• pp.49-49
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• 2010

The most commonly used numerical modelling techniques for acoustics and vibration are based on element based techniques, such as the nite element and boundary element method. Due to the huge computational eorts involved, the use of these deterministic techniques is practically restricted to low-frequency applications. For high-frequency modelling, probabilistic techniques such as SEA are well established. However, there is still a wide mid-frequency range, for which no adequate and mature prediction techniques are available. In this frequency range, the computational eorts of conventional element based techniques become prohibitively large, while the basic assumptions of the probabilistic techniques are not yet valid. In recent years, a vast amount of research has been initiated in a quest for an adequate solution for the current midfrequency problem. One family of research methods focuses on novel deterministic approaches with an enhanced convergence rate and computational eciency compared to the conventional element based methods in order to shift the practical frequency limitation towards the mid-frequency range. Amongst those techniques, a wave based prediction technique using an indirect Tretz approach is being developed at the K.U.Leuven - Noise and Vibration Research group. This paper starts with an outline of the major features of the mid-frequency modelling challenge and provides a short overview of the current research activities in response to this challenge. Next, the basic concepts of the wave based technique and its hybrid coupling with nite element schemes are described. Various validations on two- and threedimensional acoustic, elastic, poro-elastic and vibro-acoustic examples are given to illustrate the potential of the method and its benecial performance as compared to conventional element based methods. A closing part shares some views on the open issues and future research directions.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1650-1654
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• 2007

홍수와 가뭄의 증가, 수질악화, 물부족 등 물 문제 해결을 위한 대안으로 통합수자원관리에 대한 논의가 국제사회에서 활발하게 진행되고 있다. 국내에서도 많은 학자들이 수자원관리를 위한 새로운 대안으로 통합수자원관리를 주장하고 있지만, 추상적인 설명이 대부분이다. 따라서 본 연구는 통합수자원관리에 대한 정의, 원칙, 추진전략 등을 정리하여 통합수자원관리에 대한 종합적이고 체계적인 정보를 제공하고자 한다. 본 연구는 다음과 같은 흐름을 갖는다. 첫째, 수자원관리를 위한 새로운 대안으로 통합수자원관리가 등장하게 된 배경을 살펴보았다. 물문제를 해결하기 위해서는 연속적이고 상호작용하는 하나의 '시스템(係)'으로 수자원을 인식하고 관리해야 한다는 주장이 확산되고 있으며, 통합수자원관리가 그러한 인식을 갖고 있는 대안으로 등장하였다. 둘째, 통합수자원관리의 정의, 원칙 등을 조사하였다. 통합수자원관리는 자연의 지속가능성을 훼손하지 않으면서 경제적, 사회적 복지를 최대화하기 위하여 물, 토지 및 관련 자원의 조화로운 개발을 위한 과정으로 정의된다. 이러한 통합수자원관리를 위한 원칙으로는 지속가능한 수자원의 개발, 이용 및 관리원칙, 물순환 체계 고려 및 유역관리원칙, 이해당사자 참여 및 조정의 원칙, 비용부담 및 편익 균형배분의 원칙 등이 있다. 셋째, 통합수자원관리를 위한 추진방안을 제시하였다. 법제도 정비 및 부문별 통합관리강화, 수자원 관련 자료의 신뢰도 제고 및 의사결정 체계 구축, 관련계획과의 연계, 계획-실행-평가의 순환구축, 물관리 비용부담체계 구축 등이 통합수자원관리의 실행을 앞당길 수 있을 것이다. 본 연구는 통합수자원관리에 대하여 종합적인 정보를 제공하고 개선을 위한 추진방안을 제시하여 국내 수자원관리의 문제해결을 위한 기반을 구축하는 데에 기여할 것으로 기대된다. 범람범위의 1차적인 정보에서 수립할 수 있었던 계획이 가질 수 있었던 한계점을 좀 더 향상된 정보를 이용하여 극복함으로써 효율적이고 체계적인 치수 방재 계획수립이 가능할 것으로 판단된다.group) 67개, 속(family) 161개로 분류되었으며, 가장 저차단위인 통(series)이 390개 분류되었다. 또한 작물이용측면에서 실용적 분류를 실시하였는데, 논토양의 경우 보통논, 미숙논, 사질논, 습논, 염해논, 특이산성논으로 분류할 경우 각각의 분포면적이 31.9%, 23.0%, 31.9%, 9.1%, 3.9%, 0.2%이었다. 밭토양의 경우에도 보통밭, 사질밭, 미숙밭, 중점밭, 고원밭, 화산회밭으로 6개 유형으로 분류할 경우 각각의 분포면적은 41.9%, 23.3%, 17.5%, 13.9%, 1.1. 2.2% 이었다. 도시화 및 도로확대 등 다양한 토지이용 및 지형개변으로 과거의 토양정보가 많이 변경되었다. 그래서, 앞으로는 인공위성자료 및 항공사진을 이용하여 빠르고 쉽게 활용할 수 있는 토양조사 방법개발과 기 구축된 토양도의 수정, 보완 작업이 필요한 절실히 요구되고 있는 현실이다.브로 출시에 따른 마케팅 및 고객관리와 관련된 시사점을 논의한다.는 교합면에서 2, 3, 4군이 1군에 비해 변연적합도가 높았으며 (p < 0.05), 인접면과 치은면에서는 군간 유의차를 보이지 않았다 이번 연구를 통하여 복합레진을 간헐적 광중합시킴으로써 변연적합도가 향상될 수 있음을 알 수 있었다.시장에 비해 주가가 비교적 안정적인 수준을 유지해 왔다고 볼 수 있다.36.4%)와 외식을 선호(29.1%)${\lrcorner}$ 하기 때문에 패스트푸드를 이용하게 된 것으로 응답 하였으며, 남 여 대학생간에는 유의한 차이(p<0.05)가 인정되었다. 응답자의 체형은 ${\