• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.5
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• pp.286-290
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• 2005

Oxytocin (OT)-related peptide, isotocin was purified from the brain extract of conger eel (Conger myriaster) using reverse-phase, ion-exchange and size exclusion high performance liquid chromatography (HPLC). The sequence of the peptide, with a molecular weight of 967.30 Da, was determined as Cys-Tyr-Ile-Ser-Asn­Cys-Pro-Ile-Gly-$NH_2$, where the Cys between 1st and 6th residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis and MALDI-TOF mass spectrometry. The sequence was confirmed by identical elution with the purified and synthetic peptide using the HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of OT -superfamily member, isotocin. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-7}M$ on the intestinal smooth muscle of goldfish (Carassius auratus).

• Journal of Ginseng Research
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• v.31 no.3
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• pp.159-174
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• 2007

Pharmacology of Korean Red ginseng gives us unique possibility to develop new class of antiepileptic drugs today and to improve one's biological activity. The chemical structures of ginsenosides (GS) have some principal differences from well-known antiepileptic new generation drugs. The antiepileptic effect of GS was also demonstrated in all models of epilepsy in rats (young and adult), which have studied, in all models of epilepsy including status epilepticus (SE), induced by lithium - pilocarpine. In our experiments in rats new evidences on protective effects were exerted as a result of premedication by GS. Pre-treatment of several GS could induce decrease of the seizures severity and brain structural damage (by MRI), neuronal degeneration in hippocampus. Wave nature of severity of motor seizures during convulsive SE was observed during lithium-pilocarpine model of SE in rats (the first increase of seizures was 30 min after the beginning of SE and the second - 90 min after. The efficacy of treatment on SE by ginsenoside as expected was observed after no less 3 weeks by daily GS i.p. administration. It is blocked SE or significantly decrease the severity of seizures during SE. The implication of presented data is that combination of ginsenosides from Korean Red ginseng and ginseng cell culture Dan25 that could be applied for prevention of epileptical status development. However, a development of optimal ratio of different ginsenosides $(Rb_1$ Rc, Rg, Rf,) should consummate in the new antiepileptic drug development.

• The Plant Pathology Journal
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• v.37 no.3
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• pp.205-214
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• 2021

The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant's defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms.

• Advances in materials Research
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• v.12 no.4
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• pp.287-308
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• 2023

The main objective of this article is to highlight the progress made in the development of new materials that have been gradually used by humans until today. Of course, this progress must be associated with other parameters in order to guarantee sustainable development. For this, today, it has become urgent to reduce the consumption of cement by resorting to its partial or total replacement by other similar materials in order to reduce CO₂ emissions in our environment. This should certainly help to develop greener building materials. In this study, it was decided to proceed with the partial or total replacement of Portland cement type CEM II/B-L-42.5N by slate and lime that had not undergone any previous transformation. The results obtained revealed that the mortar whose substitution compared to the replacement of cement (100%) cement and sand (0/4) confers better kinetics than those of the series composed of(100%) cement and fraction rubble (0/1).

• International Journal of Knowledge Content Development & Technology
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• v.13 no.4
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• pp.95-117
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• 2023

The Digitalia MUNI ARTS - a local node of the LINDAT/CLARIAH-CZ research infrastructure at the Faculty of Arts of Masaryk University constitutes a repository in the Islandora system. It is used for long-term preservation of research data together with their research environment in the form of digital platforms. We transfer the digital outputs of humanities scholars research to the repository according to a set plan, which is based on best practice recommendations for project management and digital curation. In this paper, we present how the results of interviews with platform developers and infrastructure stakeholders translate into the curation workflow, and a resulting model for migrating digital platforms to the repository. Reflecting on three types of problems we encountered during the implementation of platforms into the repository - communication problems, problems of external dependence, and management problems - we describe a modification of the migration process. We present six recommendations for repository administrators and curators in an academic setting - holding an introductory meeting with developers, researching significant and relevant theories of knowledge domain, consulting license experts, prioritizing requirements, and preparing handover protocol and progress reports.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.261-262
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• 2002

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.342-343
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• 2002

• Structural Engineering and Mechanics
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• v.44 no.2
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• pp.257-266
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• 2012

Walls are the most important vertical load-carrying elements of masonry structures. Their bond designs are different from one country to another. This paper presents the shear effects of some structural bond designs commonly used for masonry walls. Six different bond designs are considered and modeled using finite element procedures under lateral loading to examine the shear behavior of masonry walls. To obtain accurate results, finite element models are assumed in the inelastic region. Crack development patterns for each wall are illustrated on deformed meshes, and the numerical results are compared.

• Proceedings of the Korean Aquaculture Society Conference
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• 2002.08a
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• pp.213-214
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• 2002

• Proceedings of the Korean Aquaculture Society Conference
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• 2002.08a
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• pp.241-242
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• 2002