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Electrospun Antimicrobial Polyurethane Nanofibers Containing Silver Nanoparticles for Biotechnological Applications

  • Sheikh, Faheem A.;Barakat, Nasser A.M.;Kanjwal, Muzafar A.;Chaudhari, Atul A.;Jung, In-Hee;Lee, John-Hwa;Kim, Hak-Yong
    • Macromolecular Research
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    • v.17 no.9
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    • pp.688-696
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    • 2009
  • In this study, a new class of polyurethane (PU) nanofibers containing silver (Ag) nanoparticles (NPs) was synthesized by electrospinning. A simple method that did not depending on additional foreign chemicals was used to self synthesize the silver NPs in/on PU nanofibers. The synthesis of silver NPs was carried out by exploiting the reduction ability of N,N-dimethylformamide (DMF), which is used mainly to decompose silver nitrate to silver NPs. Typically, a sol-gel consisting of $AgNO_3$/PU was electrospun and aged for one week. Silver NPs were created in/on PU nanofibers. SEM confirmed the well oriented nanofibers and good dispersion of pure silver NPs. TEM indicated that the Ag NPs were 5 to 20 nm in diameter. XRD demonstrated the good crystalline features of silver metal. The mechanical properties of the nanofiber mats showed improvement with increasing silver NPs content. The fixedness of the silver NPs obtained on PU nanofibers was examined by harsh successive washing of the as-prepared mats using a large amount of water. The results confirmed the good stability of the synthesized nanofiber mats. Two model organisms, E. coli and S. typhimurium, were used to check the antimicrobial influence of these nanofiber mats. Subsequently, antimicrobial tests indicated that the prepared nanofibers have a high bactericidal effect. Accordingly, these results highlight the potential use of these nanofiber mats as antimicrobial agents.

Dietary Optimum Phosphorus Level of Juvenile Korean Rockfish (Sebastes schlegeli)

  • Lee Sang-Min;Park Sung-Real;Kim Jeong Dae
    • Fisheries and Aquatic Sciences
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    • v.1 no.2
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    • pp.180-186
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    • 1998
  • A 10-week feeding experiment was conducted to determine the phosphorus requirement of juvenile Korean rockfish (Sebastes schlegeli). Three replicate groups of fish initially averaging 4.2g were fed the semipurified experimental diets containing graded levels of $NaH_2PO_4\;\cdot\;2H_2O$ to provide from $0.1\%$ to $1.32\%$ total phosphorus level in a flow-through seawater system. Korean rockfish muscle and casein were used as the protein sources of the basal diet. Weight gain, feed efficiency and protein retention of fish fed the $0.35\%$ phosphorus were higher than those of fish fed the $0.1\%$ phosphorus, although no significant improvements $(P>0.01)$ were observed above the level. Determined phosphorus requirement using the broken line model was found to be $0.3\%$ for weight gain. Moisture, protein and lipid contents of whole body and muscle were not affected by dietary phosphorus levels $(P>0.01)$. Lipid contents of liver in fish fed the $0.1\%$ phosphorus were lower than those in fish fed the $0.35\%$ and $1.32\%$ phosphorus $(P<0.01)$. Dietary phosphorus increased ash and phosphorus contents of the whole body, while those of bone were not affected $(P>0.01)$. The data obtained in this study indicate that a $0.3\%$ dietaryphosphorus level could be recommended for the optimum growth and efficient nutrient utilization of juvenile Korean rockfish.

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Hydrogen Absorption at a Low Temperature by MgH2 after Reactive Mechanical Grinding

  • Song, Myoung Youp;Lee, Seong Ho;Kwak, Young Jun;Park, Hye Ryoung
    • Korean Journal of Materials Research
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    • v.24 no.3
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    • pp.129-134
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    • 2014
  • Pure $MgH_2$ was milled under a hydrogen atmosphere (reactive mechanical grinding, RMG). The hydrogen storage properties of the prepared samples were studied at a relatively low temperature of 423 K and were compared with those of pure Mg. The hydriding rate of the Mg was extremely low (0.0008 wt% H/min at n = 4), and the $MgH_2$ after RMG had higher hydriding rates than that of Mg at 423 K under 12 bar $H_2$. The initial hydriding rate of $MgH_2$ after RMG at 423 K under 12 bar $H_2$ was the highest (0.08 wt% H/min) at n = 2. At n = 2, the $MgH_2$ after RMG absorbed 0.39 wt% H for 5 min, and 1.21 wt% H for 60 min at 423K under 12 bar $H_2$. At 573 K under 12 bar $H_2$, the $MgH_2$ after RMG absorbed 4.86 wt% H for 5 min, and 5.52 wt% H for 60 min at n = 2. At 573 K and 423 K under 1.0 bar $H_2$, the $MgH_2$ after RMG and the Mg did not release hydrogen. The decrease in particle size and creation of defects by reactive mechanical grinding are believed to have led to the increase in the hydriding rate of the $MgH_2$ after RMG at a relatively low temperature of 423 K.

Analysis on Residential Micro Climate of the Urban Heat Island and Oxygen Concentration in Winter (겨울철 열섬 및 산소농도의 측정을 통한 주거지별 국지기후의 특성분석)

  • Hwang Jee Wook;Kim So Chong
    • Journal of Environmental Science International
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    • v.13 no.12
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    • pp.1023-1032
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    • 2004
  • Rapid progress in urbanization has resulted in a change of the micro climate, especially in the urban area. In order to investigate the phenomenon of the heat island in the residential micro climate, a field survey was carried out by 4 sets of the residential type in Jeonju under typical winter synoptic condition. As analytic methode, it is used the comparison on the relation of the Land-to-Coverage Rate to Heat Island and Oxygen Concentration. And as a key question it is asked how stable characteristics of the micro climate will result from the survey of the Heat Island and the Oxygen Concentration, used as indicator. To ensure the trustworthy result of research, it is calculated the critical influence of the wind velocity and the Land-to-Covearage Rate. As a result of comparative analysis, it could be confirmed that the local temperatures in all sets of the residential type were higher than the average temperature in Jeonju. But the housing type A 'exclusive use for housing zone' has relativly the most stable and best living condition. On the contrary the residential type B and D has the worst toward the oxygen concentration in the time zone 9-12 a.m., which didn't reach the minimum of the oxygen concentration $20.5{\%}.$ It means that the higer the development and population density is, the worse is the situation of the Quality of Life in the residential types in accordance with the heat island and oxygon con­centration.

Isolation and Characterization of 3,4-Dichloroaniline Degrading Bacteria (3,4-Dichloroaniline 분해 미생물의 분리 및 특성)

  • Kim, Young-Mog;Park, Kun-Ba-Wui;Kim, Won-Chan;Han, Won-Sub;Yu, Choon-Bal;Rhee, In-Koo
    • Microbiology and Biotechnology Letters
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    • v.35 no.3
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    • pp.245-249
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    • 2007
  • Chloroanilines are widely used in the production of dyes, drugs and herbicides. Chloroanilines, however, are considered potential pollutants due to their toxic and recalcitrant properties to humans and other species. With the increase of necessity of bioremediation, this study was conducted to isolate the chloroanilines-degrading bacteria. A bacterium capable of growth on 3,4-dichloroaniline (DCA) was isolated by the 3,4-DCA-containing enrichment culture. The strain KB35B was identified as Pseudomonas sp. and also able to degrade several chloroanilines. The isolated strain showed high level of catechol 2,3-dioxygenase activity in the presence of 3,4-DCA. The activity of catecho1 2,3-dioxygenase was supposed to be ones of the important factors for 3,4-DCA degradation. The activity toward 4-methykatechol was 60.6% of that of catechol, while the activity toward 3-methylcatechol and 4-chlorocatechol were 27.0 and 13.5%, respectively.

Streptochlorin Isolated from Streptomyces sp. Induces Apoptosis in Human Hepatocarcinoma Cells Through a Reactive Oxygen Species-Mediated Mitochondrial Pathway

  • Shin, Dong-Yeok;Shin, Hee-Jae;Kim, Gi-Young;Cheong, Jae-Hun;Choi, Il-Whan;Kim, Se-Kwon;Moon, Sung-Kwon;Kang, Ho-Sung;Choi, Yung-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.18 no.11
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    • pp.1862-1867
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    • 2008
  • Streptochlorin is a small molecule isolated from marine Streptomyces sp. that is known to have antiangiogenic and anticancer properties. In this study, we examined the effects of this compound on reactive oxygen species (ROS) production and the association of these effects with apoptotic tumor cell death, using a human hepatocarcinoma Hep3B cell line. The results of this study demonstrated that streptochlorin mediates ROS production, and that this mediation is followed by a decrease in the mitochondrial membrane potential (MMP, ${\Delta}{\Psi}_m$), activation of caspase-3, and downregulation of antiapoptotic Bcl-2 protein. The quenching of ROS generation by N-acetyl-L-cysteine administration, a scavenger of ROS, reversed the streptochlorin-induced apoptosis effects via inhibition of ROS production, MMP collapse, and the subsequent activation of caspase-3. These observations clearly indicate that ROS are involved in the early molecular events in the streptochlorin-induced apoptotic pathway. Taken together, our data imply that streptochlorin-induced ROS is a key mediator of MMP collapse, which leads to the caspase-3 activation, culminating in apoptosis.

Dietary Supplementation with Raspberry Extracts Modifies the Fecal Microbiota in Obese Diabetic db/db Mice

  • Garcia-Mazcorro, Jose F.;Pedreschi, Romina;Chew, Boon;Dowd, Scot E.;Kawas, Jorge R.;Noratto, Giuliana
    • Journal of Microbiology and Biotechnology
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    • v.28 no.8
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    • pp.1247-1259
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    • 2018
  • Raspberries are polyphenol-rich fruits with the potential to reduce the severity of the clinical signs associated with obesity, a phenomenon that may be related to changes in the gut microbiota. The aim of this study was to investigate the effect of raspberry supplementation on the fecal microbiota using an in vivo model of obesity. Obese diabetic db/db mice were used in this study and assigned to two experimental groups (with and without raspberry supplementation). Fecal samples were collected at the end of the supplementation period (8 weeks) and used for bacterial 16S rRNA gene profiling using a MiSeq instrument (Illumina). QIIME 1.8 was used to analyze the 16S data. Raspberry supplementation was associated with an increased abundance of Lachnospiraceae (p = 0.009), a very important group for gut health, and decreased abundances of Lactobacillus, Odoribacter, and the fiber degrader S24-7 family as well as unknown groups of Bacteroidales and Enterobacteriaceae (p < 0.05). These changes were enough to clearly differentiate bacterial communities accordingly to treatment, based on the analysis of UniFrac distance metrics. However, a predictive approach of functional profiles showed no difference between the treatment groups. Fecal metabolomic analysis provided critical information regarding the raspberry-supplemented group, whose relatively higher phytosterol concentrations may be relevant for the host health, considering the proven health benefits of these phytochemicals. Further studies are needed to investigate whether the observed differences in microbial communities (e.g., Lachnospiraceae) or metabolites relate to clinically significant differences that can prompt the use of raspberry extracts to help patients with obesity.

A Multimedia Data Compression Scheme for Disaster Prevention in Wireless Multimedia Sensor Networks

  • Park, Jun-Ho;Lim, Jong-Tae;Yoo, Jae-Soo;Oh, Yong-Sun;Oh, Sang-Hoon;Min, Byung-Won;Park, Sun-Gyu;Noh, Hwang-Woo;Hayashida, Yukuo
    • International Journal of Contents
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    • v.11 no.2
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    • pp.31-36
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    • 2015
  • Recent years have seen a significant increase in demand for multimedia data over wireless sensor networks for monitoring applications that utilize sensor nodes to collect multimedia data, including sound and video. However, the multimedia streams generate a very large amount of data. When data transmission schemes for traditional wireless sensor networks are applied in wireless multimedia sensor networks, the network lifetime significantly decreases due to the excessive energy consumption of specific nodes. In this paper, we propose a data compression scheme that implements the Chinese remainder theorem to a wireless multimedia sensor network. The proposed scheme uses the Chinese Remainder Theorem (CRT) to compress and split multimedia data, and it then transmits the bit-pattern packets of the remainder to the base station. As a result, the amount of multimedia data that is transmitted is reduced. The superiority of our proposed scheme is demonstrated by comparing its performance to that of an existing scheme. The results of our experiment indicate that our proposed scheme significantly increased the compression ratio and reduced the compression operation in comparison to those of existing compression schemes.

Study of the Early Life of Kjellmaniella crassifolia and Its Growth in the Eastern Coast of the Korean Peninsula (동해안 개다시마 Kjellmaniella crassifolia Miyabe (Phaeophyta)의 초기배양과 생장특성 연구)

  • Kim, Young-Dae;Song, Hong-In;Kim, Jin-Hee;Hong, Jung-Pyo;Jeon, Chang-Yeong;Han, Hyoung-Kyun;Kim, Su-Kyoung;Kim, Dong-Sam;Kim, Myoung-Rae;Jin, Hyung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.5
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    • pp.315-322
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    • 2007
  • Kjellmaniella crassifalia Miyabe is one of the valuable seaweed crops cultured in the Korean coastal waters of the East Sea. Unfortunately recent environmental changes have adversely influenced its growth, prompting the need to develop techniques for species restoration. To obtatin biological information, the growth of the species was monitored for one year. The maximum blade length, $110{\pm}45cm$, was attained in July, and zoospores were released in November. The optimum culture conditions allowing juvenile sporophytes to grow to young blades consisted of $20{\mu}mol/m^2s\;at\;10^{\circ}C$ under which the blade length grew to $1,732.5{\pm}143.3{\mu}m$ in 42 days. Sporangial sori started to form in September, reaching 85% maturity in November and 89% maturity in December. Examining the growth of the species according to depth, the individuals growing at 5 meters grew the most, reaching a blade length of $75.9{\pm}18.9cm$ in August.

Is Koi Herpesvirus (KHV) Related to the Mass Mortality Occurring among Cultured Carp, Cyprinus carpio, in Korea?

  • Kim, Wi-Sik;Jung, Sung-Ju;Kim, Du-Woon;Kim, Seok-Ryel;Kim, Jeong-Ho;Oh, Myung-Joo
    • Fisheries and Aquatic Sciences
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    • v.13 no.1
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    • pp.79-83
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    • 2010
  • Since 1998, a new viral disease with high mortality has been consistently recorded in Korea in cultured carp, Cyprinus carpio. In this study, we investigated an epizootic of the disease that caused high mortality rates in carp obtained from 11 farms in Korea between 1999 and 2007. Assessment of koi herpesvirus (KHV) levels in diseased carp was carried out to determine if this virus was the etiologic agent of disease in this instance. High mortality rates in carp were recorded mainly in the spring and autumn at water temperatures between $19^{\circ}C$ and $24^{\circ}C$. Diseased fish typically showed surface discoloration, with a thick opaque mucus covering the body and gills. Protozoan parasites and bacteria were recovered from 7/29 (24%) and 2/26 (8%) of fish, respectively. Evidence of viral infection was marked; cytopathic effects (CPEs), characterized by cell rounding and an extended cytoplasm in fathead minnow (FHM) cells, were detected in 40/41 fish (98%). A high mortality rate (80%) resulted when supernatants of cell cultures showing CPEs were applied to previously healthy fish. KHV was detected by polymerase chain reaction in 6/41 fish (15%), but was not detected in supernatants obtained from cell cultures showing CPEs. These results suggest that KHV may not be the etiologic agent of the high mortality occurring among cultured carp in Korea; therefore, some other-as yet unidentified-infective agent must be responsible.