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Differential Regulation of the Promoter Activity of the Mouse UCP2 and UCP3 Genes by MyoD and Myogenin

  • Kim, Dong-Ho;Jitrapakdee, Sarawut;Thompson, Mary
    • BMB Reports
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    • v.40 no.6
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    • pp.921-927
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    • 2007
  • UCP2 and UCP3 are members of the uncoupling protein family, which may play roles in energy homeostasis. In order to determine the regulation of the predominant expression of UCP3 in skeletal muscle, the effects of differentiation and myogenic regulatory factors on the promoter activities of the mouse UCP2 and UCP3 genes were studied. Reporter plasmids, containing approximately 3 kb of the 5'-upstream region of the mouse UCP2 and UCP3 genes, were transfected into C2C12 myoblasts, which were then induced to differentiate. Differentiation positively induced the reporter expression about 20-fold via the UCP3 promoter, but by only 2-fold via the UCP2 promoter. C2C12 myoblasts were cotransfected with expression vectors for myogenin and/or MyoD as well as reporter constructs. The simultaneous expression of myogenin and MyoD caused an additional 20-fold increase in the reporter expression via the UCP3 promoter, but only a weak effect via the UCP2 promoter. In L6 myoblasts, only MyoD activated the UCP3 promoter, but in 3T3-L1 cells neither factor activated the UCP3 promoter, indicating that additional cofactors are required, which are present only in C2C12 myoblasts. The expression of UCP2 and UCP3 is differentially regulated during muscle differentiation due to the different responsiveness of their promoter regions to myogenin and MyoD.

Study on making tea for sensory test, quality characteristics of bitter melon

  • Lee, Sang-Chang;Kim, Dong-il
    • Journal of Evidence-Based Herbal Medicine
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    • v.2 no.2
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    • pp.25-31
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    • 2009
  • Bitter melon Tea for this research and making tea is a sensual quality research on the Characteristics. Presented based on sensory evaluation. It's not about tea culture in quantitative and systematic quantitative description of the phrase was established. The progress of the research industry, Kyungwoon University 20 of students and faculty members representing, eight of specialist personnel, total 28 people in a group was formed. Bitter melon to highlight the health and functional matter. Aspects as the development of easy-to-drink tea, processing of tea and a variant of the changes made throughout the taste and flavor. Through the study of the drinking bitter melon tea is the optimum temperature of 70-72 ${^{\circ}C}$ and not match concentration of standard evaluation and the concentration of drinking volume, to drink soften the opinion has been established. The bitter melon tea through the systematic study of the phrase describes the temperature and the concentration was established.

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Zika Virus on YouTube: An Analysis of English-language Video Content by Source

  • Basch, Corey H.;Fung, Isaac Chun-Hai;Hammond, Rodney N.;Blankenship, Elizabeth B.;Tse, Zion Tsz Ho;Fu, King-Wa;Ip, Patrick;Basch, Charles E.
    • Journal of Preventive Medicine and Public Health
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    • v.50 no.2
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    • pp.133-140
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    • 2017
  • Objectives: The purpose of this study was to describe the source, length, number of views, and content of the most widely viewed Zika virus (ZIKV)-related YouTube videos. We hypothesized that ZIKV-related videos uploaded by different sources contained different content. Methods: The 100 most viewed English ZIKV-related videos were manually coded and analyzed statistically. Results: Among the 100 videos, there were 43 consumer-generated videos, 38 Internet-based news videos, 15 TV-based news videos, and 4 professional videos. Internet news sources captured over two-thirds of the total of 8 894 505 views. Compared with consumer-generated videos, Internet-based news videos were more likely to mention the impact of ZIKV on babies (odds ratio [OR], 6.25; 95% confidence interval [CI], 1.64 to 23.76), the number of cases in Latin America (OR, 5.63; 95% CI, 1.47 to 21.52); and ZIKV in Africa (OR, 2.56; 95% CI, 1.04 to 6.31). Compared with consumer-generated videos, TV-based news videos were more likely to express anxiety or fear of catching ZIKV (OR, 6.67; 95% CI, 1.36 to 32.70); to highlight fear of ZIKV among members of the public (OR, 7.45; 95% CI, 1.20 to 46.16); and to discuss avoiding pregnancy (OR, 3.88; 95% CI, 1.13 to 13.25). Conclusions: Public health agencies should establish a larger presence on YouTube to reach more people with evidence-based information about ZIKV.

Shear strength estimation of RC deep beams using the ANN and strut-and-tie approaches

  • Yavuz, Gunnur
    • Structural Engineering and Mechanics
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    • v.57 no.4
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    • pp.657-680
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    • 2016
  • Reinforced concrete (RC) deep beams are structural members that predominantly fail in shear. Therefore, determining the shear strength of these types of beams is very important. The strut-and-tie method is commonly used to design deep beams, and this method has been adopted in many building codes (ACI318-14, Eurocode 2-2004, CSA A23.3-2004). In this study, the efficiency of artificial neural networks (ANNs) in predicting the shear strength of RC deep beams is investigated as a different approach to the strut-and-tie method. An ANN model was developed using experimental data for 214 normal and high-strength concrete deep beams from an existing literature database. Seven different input parameters affecting the shear strength of the RC deep beams were selected to create the ANN structure. Each parameter was arranged as an input vector and a corresponding output vector that includes the shear strength of the RC deep beam. The ANN model was trained and tested using a multi-layered back-propagation method. The most convenient ANN algorithm was determined as trainGDX. Additionally, the results in the existing literature and the accuracy of the strut-and-tie model in ACI318-14 in predicting the shear strength of the RC deep beams were investigated using the same test data. The study shows that the ANN model provides acceptable predictions of the ultimate shear strength of RC deep beams (maximum $R^2{\approx}0.97$). Additionally, the ANN model is shown to provide more accurate predictions of the shear capacity than all the other computed methods in this study. The ACI318-14-STM method was very conservative, as expected. Moreover, the study shows that the proposed ANN model predicts the shear strengths of RC deep beams better than does the strut-and-tie model approaches.

Estimation about Local Strength using FE-Analysis for Steel Yacht (유한요소해석을 이용한 강선요트의 국부강도 평가)

  • Park Joo-Shin;Ko Jae-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.11 no.2 s.23
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    • pp.77-82
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    • 2005
  • Previously sailing yachts or leisure yachts were mainly made from FRP(Fiber glass reinforced plastic) in the small shipbuilding, but recently there is a trend to replace it for steel or aluminum to substitute FRP for environmental friendly materials. Although It have to need a many checked item in case of hull girder strength and transverse strength normally evaluate base on calculation of class guideline so called direct calculation method. Otherwise. this method of initial structural design considered enough for safety margin on the structure. But, case of small craft must consider for evaluating local strength through rational method. In this paper, check the bow structure members for satisfying results base on allowable stress criterion of damaged bow structure by dynamic load due to slamming and bottom impact load due to pitching motion through finite element analysis. and investigate engine bed structure considering engine weight load and transverse wave load.

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Effect of Skin Cancer Training Provided to Maritime High School Students on Their Knowledge and Behaviour

  • Sumen, Adem;Oncel, Selma
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.17
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    • pp.7769-7779
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    • 2015
  • Background: This study was conducted with the purpose of evaluating the effect of skin cancer training provided to maritime high school students on their knowledge and behaviour. Materials and Methods: The study had a quasi-experimental design with pre-test and post-test intervention and control groups. Two maritime high schools located in the city of Antalya were included within the scope of the study between March and June 2013, covering a total of 567 students. Results: While the knowledge mean scores of students regarding skin cancer and sun protection did not vary in the pre-test ($6.2{\pm}1.9$) and post-test ($6.8{\pm}1.9$) control group, the knowledge mean scores of students in the experimental group increased from $6.0{\pm}2.3$ to $10.6{\pm}1.2$ after the provided training. Some 25.4% of students in the experimental group had low knowledge level and 62.2% had medium knowledge level in the pre-test; whereas no students had low knowledge level and 94.3% had high knowledge level in the post-test. It was determined that tenth grade students, those who had previous knowledge on the subject, who considered themselves to be protecting from the sun better, had higher knowledge levels and their knowledge levels increased as the risk level increased. It was found that the provided training was effective and increased positively the knowledge, attitude and behaviour levels of students in the experimental group in terms of skin cancer and sun protection. Conclusions: Along with the provided training which started to form a lifestyle, appropriate attitudes and behaviours concerning skin cancer and sun protection could be brought to students who will work in outdoor spaces and are members of the maritime profession within the risk group.

Exploring Welfare Discourse in Korea Based on M. Foucault's Power And Knowledge Relations (M. Foucault의 권력지식관계론에 기초한 한국의 복지담론 해석)

  • Seo, Jeonghoon
    • Korean Journal of Social Welfare
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    • v.67 no.4
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    • pp.79-101
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    • 2015
  • What is the role of welfare discourse? Michel Foucault suggests the power and knowledge relation that power in a particular society and period controls the society and members by creating knowledge affecting the formation of cognitive and normative systems. Having the formation of exclusions(constraint of cognition), and materiality and reality(normative system) as an analytical framework, this article attempts the exploration of welfare discourse analyses with public statements relating to welfare subjects of the four former Korean presidents. As a result, It is found that dominant epistemic system is formed by balancing welfare and growth and regarding jobs as the best welfare(the linkage of welfare-growth-employment), emphasizing individual economic responsibility and self-reliance, pursuing welfare selectivism, and excluding comprehensive welfare provisions. At the same time, it is observed that power is not always formulating systematic knowledge and that there is a gap between cognition and norm. While the Foucauldian discourse analysis provides a causal inference about low social welfare expenditure, excessive focus on the role of power as knowledge generator and infuser causes a question of how to accommodate contemporary changes into knowledge system.

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A Study on the Awareness of Academic Library Users on the Research Support Service (대학도서관 이용자의 연구지원서비스 인식에 관한 연구)

  • Nam, Young-Joon;Kim, Hee-Sun
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.23 no.4
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    • pp.369-390
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    • 2012
  • This research analyzed the type of major research and support services provided by libraries in academic institutions. The research also investigated the awareness of users using the research and support services. We found that the awareness was influenced by users' major and status. In particular, prior usage of the services had statistically significant result depending on users' major and status. On the other hand, the rank of faculty members or the year of study for students were found to not have a significant influence on the general perception of the research support services. We also found that researchers require the support services in every stages of their study. In addition, the preference of training method and research guidance for such services differed by users' major.

Citizen Participation of the Million Amenity Park (100만평문화공원 조성을 위한 주민참여활동)

  • Kim Seung-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.6 s.113
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    • pp.22-39
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    • 2006
  • This paper is the study on the activity of citizen participation in the movement of Million Amenity Park's creation which was proposed by citizen participation for large scale's flat park within city. This park was proposed by the Busan Green Plan, commission study in 1999. The location of Park site was finally proposed at Dunchi-do, Bonglim-dong, Gangseo-gu, Busan and neighbor areas. The area of park site is approximately 500ha included the surface area of West-Nakdong River. Citizen's organization for Million Amenity Park which has 3,500 members, was established in 2001 in order to compose Million Amenity Park The development processes of this study were found to have quickening period, germination period, the 1st growing period, and the 2nd growing period, and then the results of this activity was also arranged by the researcher of this study. The movement for this park was found to have a positive activity for participating and understanding all the citizens during 7 years. And then the activities for this park were as follows: every information, fund-raising campaign, organization, purchase of park site, contribution of purchasing park site to Busan city. The results of this study, through the movement of citizen's participation such as this park movement were 1) respectively found to have the settlement in the movement of NGO, 2) the possibility of large scale's park fostering by public and civil partnership, and 3) the model presentation of frontier park in the citizen participation's types which will be able to introduce the development of city.

What do patients with psoriasis think about the causes of their disease and use for psoriasis?

  • Gonul, Muzeyyen;Cakmak, Seray Kulcu;Gul, Ulker;Biyikli, Zeynep
    • CELLMED
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    • v.2 no.1
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    • pp.8.1-8.4
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    • 2012
  • There are few reports about the beliefs of psoriasis patients related to their disorder and therapy modalities except for the medical ones. We aimed to investigate the beliefs of psoriasis patients about their disorders, where they had attended therapies unrelated to dermatologists and what modality of unconventional therapies they used for psoriasis in Turkey. The study was performed on 141 psoriasis patients. The age, sex, education status and the number of visits to the dermatology clinics were recorded. How the patients decided to visit the dermatology clinic (themselves, directed by others), where they attended for treatment of psoriasis outside of the dermatologist, what they think about the causes of their disease and what they used for treatment of psoriasis were asked. The mean age of the patients was $33.44{\pm}15.13$ years. The patients whose ages were over 30 years significantly more went to dermatology clinics. 68.1% of the patients attended the dermatology clinic based on the individual decision, 18.4% were directed by family members and 5.7% by friends. 45.4% of the patients believed that stress was the cause of their disease. 54.6% of the patients had used at least one type of unconventional therapy for psoriasis. The most frequently used unconventional therapy forms were herbal (24.1%) and spiritual therapies (22%). One conclusion is psoriasis patients need education about their diseases. Dermatologists should have information about the tendencies of the patients and approach the patients with sensitivity and empathy and give objective knowledge about psoriasis.