• 제목/요약/키워드: FRT

검색결과 188건 처리시간 0.031초

Yeast의 FLP/FRT 시스템을 이용한 BmNPV의 유전자 재조합 (Construction of Recombinant Bombyx mori Nuclear Polyhedrosis Virus Using a FLP/FRT System of Yeast, Saccharomyces cerevisiae 2$\mu$m plasmid)

  • 강석우;윤은영
    • 한국잠사곤충학회지
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    • 제40권1호
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    • pp.52-59
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    • 1998
  • For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.

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Correlation of Berg Balance Scale and Functional Reach Test

  • Park, Eun-Young;Kim, Won-Ho
    • 한국전문물리치료학회지
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    • 제14권4호
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    • pp.28-34
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    • 2007
  • The purposes of this study were to provide the basic data and investigate the reliability of functional reach test and identify correlation of Berg balance scale (BBS) and functional reach test (FRT). The subjects were twenty healthy young adults and forty-five over 65 years old in order to compare balance ability. These data were analyzed by independent t-test and Pearson's correlation test using SPSS WIN 10.0. The results were as follows. Intrarater reliability coefficients of FRT was .976 and interrater was .942. FRT was significantly correlated with age, height, and BBS (p<.05). There were no significant differences in FRT and BBS by sex. There was significant difference in reach distance between below 74 elderly and above in FRT. FRT is very reliable test for balance and significantly correlated with BBS. Therefore, it is suggested that FRT is a clinically useful tool to substitute for BBS measuring balance ability in the elderly.

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Ferritin 유전자 전이 효모(Saccharomyces serevisiae)의 급여가 닭의 생산성, 장기 및 계란의 철분함량에 미치는 영향 (Effects of Feeding Ferritin Gene Transferred Yeast (Saccharomyces serevisiae) on Performance, Iron Concentration in Organs and Egg of Chickens)

  • 유병선;박재홍;김대혁;류경선
    • 한국가금학회지
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    • 제30권4호
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    • pp.245-251
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    • 2003
  • Ferritin 유전자 이식 효모 (FRT, Saccharomyces cerevisiae)를 생균제로 급여시에 육계와 산란계의 생산성과 장기 및 난황의 철분 함량에 미치는 영향을 구명하기 위하여 3회의 사양실험을 실시하였다. FRT의 급여효과는 일반 효모 (W0)와 20mM의 구연산 철을 첨가한 배지에서 배양한 효모(W20)의 급여시와 비교하였다. 실험 1에서는 철분을 첨가급여구(75mg/kg; Fe75), 무첨가구 (Fe0) 효모의 급여(무첨가, W0, FRT)가 육계의 생산성과 장기의 철분함량에 미치는 영향을 구명하기 위하여 1일령 육계 수컷 420수를 이용하여 5주간 사양실험을 실시하였다. 매주 증체량과 사료섭취량, 사료요구율을 측정하였다. 실험 2에서는 33주령 이사브라운 산란계 15수를 산란케이지에 개체수용하여 대조구와 W0, FRT 사료를 3주간 급여하였다. 실험 3은 45주령 이사브라운 산란계 24수를 산란케이지에 개체수용하여 1주간 기초사료를 급여한 뒤 시험사료(대조구, W0, W20, FRT)를 3주간 급여하였다. 모든 실험의 종료시 간과 심장, 비장, 경골의 철분함량을 측정하였으며 주간별로 난황의 철분함량을 측정하였으며 주간별로 난황의 철분함량을 측정하였다.(Expt 2, 3) 실험 1과 2에서 효모의 급여량은 사료에 $1{\times}10^8$cfu/kg이었으며 철분함량은 세포 건물기준 500mg/kg이었다. 실험 3에서는 사료에 $2{\times}10^{10}$cfu/kg을 첨가하였으며 철분함량은 1000mg/kg이었다. 실험 1에서 Fe75의 급여는 Fe0에 비해 증체량이 현저히 증가하였다.(P<0.05). 실험 3에서 FRT의 급여는 간과 비장의 철분함량을 증가시키는 경향을 보였으나 경골의 철분함량은 감소하는 경향을 보였다. 이상의 결과에서 FRT의 급여는 생균제로서 육계와 산란계의 생산성과 장기의 철분함량을 개선하지 못하였다.

Flavonoids of Rosa roxburghii Tratt Act as Radioprotectors

  • Xu, Ping;Zhang, Wen-Bo;Cai, Xin-Hua;Lu, Dan-Dan;He, Xiao-Yang;Qiu, Pei-Yong;Wu, Jiao
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권19호
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    • pp.8171-8175
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    • 2014
  • Background: To study the radioprotective effects of flavonoids from Rosa roxburghii Tratt (FRT). Materials and Methods: The radioprotective effects of FRT were investigated by examining cell viability, 30-day survival of mice and the number of colony-forming units in spleen (CFU-S) after total-body 60Co irradiation. Results: The survival rates of irradiated cells gradually increased with increasing concentrations of FRT. The survival rate was the highest at 87% with a concentration of $30{\mu}g/mL$. Pretreatment with FRT was needed to realize its radioprotective activity in mice at the dose of 60 mg/kg. With the increasing doses of 30 mg/kg, 60 mg/kg and 120 mg/kg, the numbers of CFU-S increased, and were significantly different compared with the control group. Conclusions: Pretreatment with FRT prior to irradiation resulted in significantly higher cell survival at 24 h after 5 Gy radiation, increased 30-day survival in mice after exposure to a potentially lethal dose of 8 Gy, and resulted in a higher number of CFU-S in mice after exposure to a dose of 6 Gy. These results collectively indicate that FRT is an effective radioprotective agent.

분수차 퓨리에 변환을 위한 평판집적 광학계 구현 (Planar integrated optics for implementation of fractional fourier transform)

  • 박선택;김필수;오차환;송석호
    • 한국광학회지
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    • 제7권4호
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    • pp.333-340
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    • 1996
  • 기존 퓨리에 변환의 일반화된 형식인 분수차 퓨리에 변환을 평판집적 광학계로 구현하였다. 구현된 평판집적 광학계는 동일한 2차원 입력패턴에 대해 서로 다른 분수차를 갖는 4개의 FRT의 결과를 얻을 수 있는 구조를 가지며 모든 부품들은 레이저 빔 묘화장치를 이용하여 하나의 유리판에 동시에 제작되었다. FRT 실험결과를 계산치와 비교 분석함으로써 평판집적 광학계가 기존의 자유공간 광학계가 갖는 3차원적 광신호 전달 특성과 이에 따른 높은 신호의 공간대역폭을 가지고 매우 정확한 FTR를 수행할 수 있음을 검증하였다.

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스위스볼 요부안정화운동에 따른 뇌졸중 환자 하지의 경직도와 균형 분석 (Analysis of Spasticity and Balance of Lower Extremity on Swiss Ball Lumbar Stabilization Exercise(LSE) in Patients with Stroke)

  • 고대식;김찬규;정대인
    • 한국콘텐츠학회논문지
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    • 제11권3호
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    • pp.262-270
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    • 2011
  • 본 연구는 swiss ball LSE에 따른 하지 경직도, 균형 및 보행에 관한 효과를 알아보고자 BBS, FRT, 수정된 Ashworth 척도(MAS), TUG 측정 분석 하였다. 대상자는 H병원에서 입원 또는 통원치료를 받고 있는 뇌졸중 환자 중 선정기준에 적합한 34명을 고전적 신경물리치료군 17명, swiss ball LSE 실시군에 17명씩 할당하여 하지의 균형능력은 BBS와 FRT, 경직도는 MAS, 보행능력은 TUG를 사용하여 비교 분석하였다. Swiss ball LSE군에서 BBS와 FRT는 유의하게 증가하였으며, MAS와 TUG는 유의하게 감소하였다. 또한 군간의 비교에서는 FRT와 MAS에서 유의한 차이가 나타났다. 결과적으로 swiss ball LSE는 뇌졸중 환자의 하지 근경직, 균형능력 및 보행능력을 회복시킬 수 있는 것으로 사료된다.

노인요양병원 입원환자의 TUG, FRT와 SPPB와의 상관관계 연구 (Correlations between SPPB, FRT, and TUG in Hospitalized Frail People - The Timed Up and Go test, the Functional Reaching Test, and the Short physical performance battery -)

  • 박래준;손호희;조정선;오현주;이현희;이문환;김성길
    • The Journal of Korean Physical Therapy
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    • 제23권2호
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    • pp.17-21
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    • 2011
  • Purpose: The purpose of this study was to determine the correlations, if any, between SPPB (Short physical performance battery), FRT (Functional reach test), and TUG (Timed up and go test) in hospitalized frail people. Methods: Seventy frail elderly patients (male/female: 16/54) participated in this study. All subjects could walk with or without an assisting device and they had no mental problems. Subjects were scored on physical functioning using the SPPB, FRT, and TUG. The data were analyzed using Pearson Correlation Coefficients. Results: There was a statistically significant difference between (a) SPPB and (b) FRT (p<0.01) and TUG (p<0.01). SPPB and FRT scores showed a positive correlation (r = 0.38) and SPPB and TUG scores showed a negative correlation (r = -0.56). Conclusion: SPPB, FRT, TUG scores are correlated with each other. From these results, we conclude that the SPPB is a useful method for predicting walking, balance, and physical function in frail elderly people.

다당류 및 TGase를 처리한 동결 무지개송어(Oncorhynchus mykiss)를 활용한 라이스페이퍼롤의 품질 특성 (Quality Characteristics of Rice Paper Roll with Frozen Rainbow Trout Oncorhynchus mykiss Meat Treated with Polysaccharide and TGase)

  • 박혜민;이종봉;안병규;이우진;인정진;한형구;손승아;배연주;심길보
    • 한국수산과학회지
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    • 제56권5호
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    • pp.585-595
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    • 2023
  • This study was conducted to optimize the rice paper roll processing conditions with frozen rainbow trout Oncorhynchus mykiss meat (RPR-FRT) treated with polysaccharide and TGase using a response surface methodology and to examine their quality characteristics. The RSM results for the RPR-FRT showed that the optimum condition for the garlic-pepper mixture was 21.2 g and that for starch was 22.6 g based on 150 g of RPR-FRT. The RPR-FRT contained 58.47 g/100 g of moisture, 8.57 g/100 g of crude protein, 3.28 g/100 g of crude lipid, and 1.00 g/100 g of ash. The vitamin E content was 1,010.91 ㎍/100 g. Based on their contents, the samples could be considered good supplements for P, Cr, and Se. The RPR-FRT contained unsaturated fatty acids (75.84%), DHA (10.33%), and EPA (2.56%). Anserine, arginine, glycine, and taurine accounted for 41.93%, 11.27%, 7.13%, and 7.00% of free amino acids in the RPR-FRT, respectively. The sulfur compounds in the RPR-FRT constituted 73.16% of the total flavor compounds. The RPR-FRT prepared using the optimum conditions was superior in masking off-flavor and showed improved nutritional content.

FRT 디자인을 위한 가시적, 비가시적 요인 구축 (Establishment of Visible & Invisible Factors for FRT Design)

  • 한석우
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2004년도 추계학술대회 논문집
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    • pp.1701-1706
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    • 2004
  • FRT design aims at high-tech vehicles of high efficiency and cleanness, it must materialize the meaning and role as new added value by applying car design aesthetics of a new future-oriented concept. In addition, different from ordinary vehicle design, FRT involves invisible factors such as pleasantness, safety, accessibility, controllability, movability, conveniency, culture, identity, brand image and fashionableness. Because FRT vehicles are transportation means used by various groups of people, they must be approached with the barrier-free design concept. Therefore, in order to enhance the potential of future transportation culture emphasizing creativity based on diversity, it is important to establish core design factors that create new added values and to apply new concepts to their roles.

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도시형 FRT Exterior Design 연구 (A study of urban FRT's Exterior Design)

  • 김성남;김상중;김창현
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2004년도 추계학술대회 논문집
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    • pp.1757-1764
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    • 2004
  • It is the urgent task of induce the inhabitants to utilize the public transportation instead of the personal transportation to dissolve the traffic jam. But construction of the subway, the representative substitute means, demands a great of investment expense including various kinds of expenses. So the technical development and research of standardization of FRT(Fuel cell Rubber tired Train), the eco-friendly public transportation system low in construction expenses, effective, excellent in approach as well as in traffic, are propelled positively. This project wants to propose exterior design FRT in which original creative methods are utilized with the target of development and construction of FRT which coincide with anthropocentric eco-friendly traffic system. Utilizing the proposed style as the basic materials, this project wants to grasp difficulties, and to establish the way to solve and develop them.

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