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Whitening and inhibiting NF-κB-mediated inflammation properties of the biotransformed green ginseng berry of new cultivar K1, ginsenoside Rg2 enriched, on B16 and LPS-stimulated RAW 264.7 cells

  • Xu, Xing Yue;Yi, Eun Seob;Kang, Chang Ho;Liu, Ying;Lee, Yeong-Geun;Choi, Han Sol;Jang, Hyun Bin;Huo, Yue;Baek, Nam-In;Yang, Deok Chun;Kim, Yeon-Ju
    • Journal of Ginseng Research
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    • v.45 no.6
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    • pp.631-641
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    • 2021
  • Background: Main bioactive constituents and pharmacological functions of ripened red ginseng berry (Panax ginseng Meyer) have been frequently reported. Yet, the research gap targeting the beneficial activities of transformed green ginseng berries has not reported elsewhere. Methods: Ginsenosides of new green berry cultivar K-1 (GK-1) were identified by HPLC-QTOF/MS. Ginsenosides bioconversion in GK-1 by bgp1 enzyme was confirmed with HPLC and TLC. Then, mechanisms of GK-1 and β-glucosidase (bgp1) biotransformed GK-1 (BGK-1) were determined by Quantitative Reverse Transcription-Polymerase Chain Reaction and Western blot. Results: GK-1 possesses highest ginsenosides especially ginsenoside-Re amongst seven ginseng cultivars including (Chunpoong, Huangsuk, Kumpoong, K-1, Honkaejong, Gopoong, and Yunpoong). Ginseng root's biomass is not affected with the harvest of GK-1 at 3 weeks after flowering period. Then, Re is bioconverted into a promising pharmaceutical effect of Rg2 via bgp1. According to the results of cell assays, BGK-1 shows decrease of tyrosinase and melanin content in α-melanocyte-stimulating hormone challenged-murine melanoma B16 cells. BGK-1 which is comparatively more effective than GK-1 extract shows significant suppression of the nuclear factor (NF)-κB activation and inflammatory target genes, in LPS-stimulated RAW 264.7 cells. Conclusion: These results reported effective whitening and anti-inflammatory of BGK-1 as compared to GK-1.

A study on the honeycomb entry and exit counting system for measuring the amount of movement of honeybees inside the beehive (벌통 내부 꿀벌 이동량 측정을 위한 벌집 입·출입 계수 시스템 연구)

  • Kim, Joon Ho;Seo, Hee;Han, Wook;Chung, Wonki
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.4
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    • pp.857-862
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    • 2021
  • Recently, rapid climate change has had a significant impact on the bee ecosystem. The decrease in the number of bees and the change in the flowering period have a huge impact on the harvesting of beekeepers. Accordingly, attention is focused on smart beekeeping, which introduces IoT technology to beekeeping. According to the characteristics of beekeeping, it is impossible to continuously observe the beehive in the hive with the naked eye, and the condition of the hive is mostly dependent on knowledge from experience. Although a system that can measure partly through sensors such as temperature/humidity change inside the hive and measurement of the amount of CO2 is applied, there is no research on measuring the movement path and amount of movement of bees inside the beehive. Part of the migration of honeybees inside the hive can provide basic information to predict the most important cleavage time in beekeeping. In this study, we propose a device that detects the movement path of bees and measures and records data entering and exiting the hive in real time. The device proposed in this study was developed according to the honeycomb standard of the existing beehive so that beekeeping farms could use it. The development method used a photodetector that can detect the movement of bees to configure 16 movement paths and to detect the movement of bees in real time. If the measured honeybee movement status is utilized, the problem of directly observing the colony with the naked eye in order not to miss the swarming time can be solved.

Effects of Different Pruning Time on Bush Growth and Fruit Production of Southern Highbush Blueberry 'Scintilla' Cultivated in a Heated Plastic House (전정시기가 가온재배 남부하이부쉬 블루베리 '신틸라'의 신초 생장과 과실 생산에 미치는 영향)

  • Cheon, Mi Geon;Lee, Seo Hyoun;Park, Kyung Mi;Choi, Seong-Tae;Hwang, Yeon Hyeon;Chang, Young Ho;Kim, Jin Gook
    • Journal of Bio-Environment Control
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    • v.30 no.3
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    • pp.183-187
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    • 2021
  • Appropriate pruning is very important for southern highbush blueberries cultivated in a heated plastic house to control their severe crowding within the bush canopy after harvest. Pruning treatments at different times were evaluated to find out an appropriate pruning time to southern highbush 'Scintilla' cultivation with heating. Seven-year-old (2018) bushes, grown in 180-L containers, were summer-pruned on May 20 (35-39 days after harvest) and June 20, or dormant-pruned on December 20 (5 days before flowering), consecutively in both 2018 and 2019 removing 30% of the total woods. May pruning activated occurrence of shoots the following years, increasing number of shoot by 17 to 49% and total shoot length by 18 to 32% compared with those of the dormant pruning. Fruit characteristic was not significantly affected by different pruning times the previous year. The first year pruning treatment did not influence the yield the following year, but the second year consecutive May pruning significantly increased yield per bush by 7% compared with the dormant pruning. The results indicated that summer pruning in May could be favorable to promote shoot growth and to maintain stable yield.

Development of Plant Phenology and Snow Cover Detection Technique in Mountains using Internet Protocol Camera System (무인카메라 기반 산악지역 식물계절 및 적설 탐지 기술 개발)

  • Keunchang, Jang;Jea-Chul, Kim;Junghwa, Chun;Seokil, Jang;Chi Hyeon, Ahn;Bong Cheol, Kim
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.4
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    • pp.318-329
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    • 2022
  • Plant phenology including flowering, leaf unfolding, and leaf coloring in a forest is important to understand the forest ecosystem. Temperature rise due to recent climate change, however, can lead to plant phenology change as well as snowfall in winter season. Therefore, accurate monitoring of forest environment changes such as plant phenology and snow cover is essential to understand the climate change effect on forest management. These changes can monitor using a digital camera system. This paper introduces the detection methods for plant phenology and snow cover at the mountain region using an unmanned camera system that is a way to monitor the change of forest environment. In this study, the Automatic Mountain Meteorology Stations (AMOS) operated by Korea Forest Service (KFS) were selected as the testbed sites in order to systematize the plant phenology and snow cover detection in complex mountain areas. Multi-directional Internet Protocol (IP) camera system that is a kind of unmanned camera was installed at AMOS located in Seoul, Pyeongchang, Geochang, and Uljin. To detect the forest plant phenology and snow cover, the Red-Green-Blue (RGB) analysis based on the IP camera imagery was developed. The results produced by using image analysis captured from IP camera showed good performance in comparison with in-situ data. This result indicates that the utilization technique of IP camera system can capture the forest environment effectively and can be applied to various forest fields such as secure safety, forest ecosystem and disaster management, forestry, etc.

Impact of Climate Change on Yield and Canopy Photosynthesis of Soybean (RCP 8.5 기후변화 조건에서 콩의 군락 광합성 및 수량 반응 평가)

  • Wan-Gyu, Sang;Jae-Kyeong, Baek;Dongwon, Kwon;Jung-Il, Cho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.4
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    • pp.275-284
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    • 2022
  • Changes in air temperature, CO2 concentration and precipitation due to climate change are expected to have a significant impact on soybean productivity. This study was conducted to evaluate the climate change impact on growth and development of determinate soybean cultivar in the southern parts of Korea. The high temperature during vegetative period, which does not accompany the increase of CO2 concentration, increased the canopy photosynthetic rate in soybean, but after flowering, the high temperature above the optimal ranges interrupts the photosynthetic metabolism. In yield and yield components, high temperature reduced both the pod and seed number and single seed weight, resulting in a reduction of total seed yield. On the other hand, the increase in CO2 concentration dramatically increased the canopy photosynthetic rate over the whole growth period. In addition, high CO2 concentration increased the number of pods and seeds, which had a positive effect on total seed yield. Under concurrent elevation of air temperature and CO2 concentration, canopy photosynthesis increased significantly, but enhanced canopy photosynthesis did not lead to an increase in soybean seed yield. The increase in biomass and branch by enhanced canopy photosynthesis seems to be attributed to an increase in the total number of pods and seeds per plant, which compensates for the negative effects of high temperature on pod development. However, Single seed weight tended to decrease rapidly by high temperature, regardless of CO2 concentration level. Elevated CO2 concentration did not compensate for the poor distribution of assimilations from source to sink caused by high temperature. These results show that the damage of future soybean yield and quality is closely related to high temperature stress during seed filling period.

Morphological Characteristics Based on Phenotypes of Flower and Fruit in a New Variety of Anthoxylum schinifolium (산초나무(Zanthoxylum schinifolium) 신품종의 꽃과 열매에 기반한 형태적 특성 분석)

  • An, Mi Yun;Song, Hyun Jin;Kim, Ki Yoon;Lee, Ro Young;Yang, Byeong Hoon;Han, Chi Beok
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.464-470
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    • 2022
  • In this study, the morphological characteristics of flower and fruit in five varieties (Hancho 915ho, 930ho, 10ho, 1020ho, 1030ho) from Zanthoxylum schinifolium were investigated as new varieties with different fruiting seasons. Qualitative and quantitative characteristics were investigated, spectively. For quantitative characteristics can be measured numerically, such as size and number, the varieties were identified using ANOVA and Scheffe's multiple range test. In addition, the validity of whether the quantitative characteristics of five varieties can be explained by factor analysis was reviewed. Using UPGMA, the relationship between the five varieties was confirmed by dendrogram. As a result of ANOVA and Scheffe's multiple range test there was significant difference in inflorescence width and female flower length. It had a high contribution to distinguishing varieties, which was similar to the PCA results using factor analysis. As a result of dendrogram using cluster analysis, Hancho 915ho, 1020ho and 1030ho were similar, but there was a distinct difference in the characteristics of different flowering and fruiting periods, confirming that they were different varieties.

Relationship between Seed Weight and Agronomic Traits in Korean Native Soybeans (재래종 대두의 종실중과 저형질과의 관계)

  • Song, Hi-Sup;Lee, Young-Il;Kwon, Shin-Han;Song, In-Man;Kwon, Kyeong-Hak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.28-33
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    • 1990
  • The present study was conducted to evaluate agronomic characters of the soybean germplasm which was collected from the sites covering southern parts of Korean peninsula. The total collections of 2748 native soybean varieties were grouped by eight levels of seed weight and the correlation between the seed weight and the other agronomic traits were determined. Seed weight was ranged from 7. 3g to 48. 4g with a great variation and averaged 23. 5g. Seed size of the many local lines were relatively large than that of foreign varieties. Frequency distribution of the germplasm for seed weight were 2.2% in Group 1 (below 10.0g), 14.7% in Group 2 (10.1-15.0g), 11.7% in Group 3 (15.1-20.0g), 26.2% in Group 4 (20.1-25.0g), 30.2% in Group 5 (25.1-30.0g). 10.6% in Group 6 (30.1-35.0g), 3.3% in Group 7 (35.1-40.0g) and 1.1% in Group 8 (over 40.0g). There was positive correlationship between seed weight and maturity or fruiting period, while there was negative correlationship between the seed weight and the other traits such as days to flowering, plant height, and number of nodes and pods. While 100 seed weight of 464 lines (16.9% of total lines) were below 15g, and those are adequate for the bean sprouts. The fruiting period of large seeded lines was longer, however the number of pods per plant of those were less lines than that of small seeded lines.

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Effects of Early Planting and Polyethylene Mulch on Soil for Early Maturing Soybean in Kyeonggi Area (경기지역에서 대두콩에 대한 피복재배효과)

  • Song, Su-Hyeun;Kim, Sung-Ki;Park, Kyeong-Yeol;Kim, Byeong-Hyeon;Ree, Dong-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.1
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    • pp.112-118
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    • 1987
  • This study was carried out to investigate the effects of early planting and polyethylene film (P.E) mulch on early maturing soybean of Korean native cultivar Kyeonggi No. 114 in the Kyeonggi area. It was planted four times at the ten days interval from April I to April 30 in 1985 and 1986. The average soil temperature was 1-5$^{\circ}C$ higher under the polyethylene film mulch condition than the conventional cultural practice condition. Soil moisture content also was higher under P.E mulch condition. Days to emergence was shortened 5-16 days and days to flowering was shortened 3-5 days. The fresh pod yield was increased 12% more at the planting date of April 1 and April 10. Income was increased 70, 83, more at the planting date of April 1 and April 10 under the polyethylene mm mulch conditions.

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Effects of Date and Degrees of Defoliation on Seed Yield and Fatty Acid Composition of Perilla(Perilla ocymoids L.) (채엽시기와 정도가 들깨의 종실수량과 지방산조성에 미치는 영향)

  • Bin, Young-Ho;Choe, Zhin-Ryong;Yang, Min-Suk;Kim, Seok-Hyeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.182-188
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    • 1988
  • In order to find out the effects of the time and degree of defoliation on the seed yield, oil content and fatty acid composition of perilla seed, two cultivars, Namhae and Geumnung, were grown in two years, 1985 and 1987. Defoliation was initiated after 110 days from sowing with two-week interval. Defoliation degree was manipulated into four levels i.e. 0, 25, 50 and 75 per cent based on the total leaf area per plant. The results are summarized as follows: Flowering date, maturing date, culm length, culm diameter and branch numbers were not significantly influenced by the defoliation time and degrees, but by the cultivars. The higher levels of defoliation was made, the lower seed yield and 1000-seed were obtained, and either the earlier or the later time of defoliation, the less influence on seed yield were observed for both cultivars. Severe defoliation caused a significant decrease in oil content. The composition of unsaturated and saturated fatty acids was significantly decreased by severe defoliation (above 25 per cent). In conclusion, with a view to achieving higher seed yield and oil content with good quality, it is advisable to defoliate perilla leaf less than 25 per cent based on total leaf area at either or later growth stages.

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Effect of Vinyl-mulching Culture on Growth and Yield of Cotton( Gosspium hirsutum L.) (비닐피복이 모화의 생육 및 수량에 미치는 영향)

  • Lee, Jung-Il;Park, Yong-Hwan;Chung, Kyu-Yong;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.4
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    • pp.434-439
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    • 1986
  • This experiment was conducted to explore the cultural method capable of raising of picked cotton ratio and more high-yielding in the middle part of Korea where growth period of cotton is short. By early vinyl-mulching culture, emergence date was earlier, flowering and boll opening were shorter than non-mulching, especially boll opening date was earlier about 8 to 15 days. Plant height was longer by vinyl-mulching, fruiting branches and number of bolls per plant at vinyl-mulching of the 10th April produced more twice than non-mulching of the 10th May. Picked cotton ratio was 79% at non-mulching of the 10th May, while viny-mulching of the 10th April produced most of picked cotton as picked cotton ratio 99%. At vinyl-mulching of the 10th April, lint yield increased more 76-90% than non-mulching. Staple length was some longer and also content of linolenic acid of cotton seed oil was higher than non-mulching.

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