• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.23-23
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• 2019

Cypripedium is an orchid genus of over 50 species widely distributed in the northern temperate zone, with a few taxa extending central America and the Himalayas and the southwesternmost tip of China in Asia. Of the known species approximately two thirds are found in China. In Korea, 4 species were reported to find with rare popularity ; C. macranthos, C. guttatum, C. japonicum, and C. calceolus. The seed has a firm brown fusiform testa with an opening at one end where the micropyle of the ovule was situated. Cypripedium has small, fusiform, wind-dispersed seeds that weigh about $1{\sim}2{\mu}g$ and range from 0.1 to 2 mm long and 0.07 to 0.4 mm across. The germination of Cypripedium was previously thought to be hard to cultivate in one's garden and impossible to propagate artificially, nowadays lab-propagated seedlings are readily available in EU or United States. We had already reported the successful micropropagation of lady's slipper orchids with green pod sowing methods. The collected Cypripedium species and hybrids were planted in Korea National Arboretum (KNA) on Oct. 2018. The 11 species including C. candidum and the 31 hybrids such as C. Ingrid were introduced from Germany with CITES certification. The lady's slipper orchids bloomed on May to June, and the flowers were pollinated in the same species or hybrids to get seeds for in vitro germination on 2~3 days after fully flowering. The green pods were collected after 49~70 days after pollination. The 6 species of Cypripedium seeds were easily germinated on the POM within 70 days after sowing, and the 12 hybrids PLBs were observed with small roots. We had established the scheme of lady's slipper orchids germination in 3 months with green pods, and get the possibility of seedling in 10 months of sowing in vitro.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.50-50
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• 2019

잇꽃(Carthamus tinctorius L.)은 국화과에 속하는 두해살이 초본 식물로 한자명은 홍화(紅花), 영명으로는 Safflower를 사용하고 있다. 잇꽃의 원산지는 이집트, 아프카니스탄 등의 서남아시아가 원산지로 알려져 있으며, 우리나라의 잇꽃 재배면적은 2010년 39 ha 수준이 재배되다가 2016년에는 67 ha로 재배면적이 증가한 것으로 보고되고 있다. 우리나라에서 잇꽃 이용은 잇꽃 종실을 위주로 이용되고 있으나 일본의 경우 잇꽃의 경관적 가치를 활용하여 산형현(山形縣)에서는 홍화축제가 매년 개최되고 있으며, 미국 등에서는 잇꽃의 저온압착유가 기능성 건강식품류로 판매되고 있다. 본 연구는 일조시수가 짧은 표고 500 m의 준고랭지에서 잇꽃의 경관적 가치 활용도를 평가하기 위해 차광조건에 따른 개화특성 및 종실 수량성을 평가하기 위해 수행되었다. 비가림 하우스에서 5월 9일 파종후 생육 54일차인 7월 5일 개화기에 지상부 발육량을 조사한 결과 차괄비율이 강해질수록 엽장폭비가 감소하여 세장형에서 장타원형으로 성장함을 알수 있었고, 분지수는 30% 차광조건에서 증가하는 경향을 보였다. 같은 시기에 조사한 엽면적에서는 30% 차광에서 증가하다가 차광비율이 높아질수록 감소하였으며, 식물체당 총 착화수는 30% 차광조건에서 20.3화/주로 가장 많았고 2차 화경의 착화수도 많은 경향을 보였다. 착생된 꽃의 개화단계를 3등급으로 구분하여 비율을 조사한 결과 차광 조건에서는 개화 2, 3단계의 비율이 무차광에 비해 낮아 개화가 지연됨을 알수 있었고, 건조한 관상화의 색도에서는 차광비율이 높아질수록 b 값이 증가하여 황색값이 높아지는 것을 알수 있었다. 파종 후 생육 94일 차인 8월 13일 종실을 수확하여 식물체당 100립중을 조사한 결과 무차광 4.9 g에 비해 차광조건에서는 감소하는 경향을 보였고, 종실수량성에서도 무차광에서 201.1 kg/10 a로 가장 높아 차광조건에서 잇꽃 재배는 개화지연에 의한 등숙률의 감소로 종실수량성이 감소하는 것으로 판단되었다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.3
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• pp.246-268
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• 2019

Soybean [Glycine max (L.) Merr.] is a species of legume native to East Asia. The interactions between climatic conditions and genetic characteristics are known to affect the agricultural performance of soybean. Therefore, the present investigation was conducted to identify the main elements affecting the agricultural performances of 11 soybean varieties/lines from China [Harbin ($45^{\circ}12^{\prime}N$), Yanji ($42^{\circ}53^{\prime}N$), Dalian ($39^{\circ}30^{\prime}N$), Qingdao ($36^{\circ}26^{\prime}N$)] and the Republic of Korea [Suwon ($37^{\circ}16^{\prime}N$), and Jeonju ($35^{\circ}49^{\prime}N$)]. The days to flowering (DTF) of soybeans with the e1-nf and e1-as alleles and the E1e2e3e4 genotype, except in 'Keumgangkong', 'Tawonkong', and 'Duyoukong', were relatively short compared to those of soybeans with other alleles. Although DTF of the soybeans was highly correlated with all climatic conditions [negative: precipitation, average temperature (AVT), accumulated temperature; positive: day-length (DL)], days to maturity and 100-seed weight of the soybeans showed no significant correlation with any climatic conditions. The soybeans with a dominant Dt1 allele, except 'Tawonkong', had the longest stem length (STL). Moreover, STL of the soybeans grown in the test fields showed a positive correlation with only DL; however, the results of our chamber test that was conducted to complement the field tests showed that STL of soybean was positively affected by AVT and DL. Although soybean yield (YLD) showed positive correlations with latitude and DL (except L62-667, OT89-5, and OT89-6), the response of YLD to the climatic conditions was cultivar-specific. Our results show that DTF and STL of soybeans grown in six different latitudes are highly affected by DL, and AVT and genetic characteristic also affect DTF and STL.

• Sijohaknonchong
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• v.24
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• pp.259-278
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• 2006

In this study, I've researched the sijo written by Yukdang, Choi, Nam Sun, and Manhae. Han, Yong Woon who were the leading intellectual and the pioneers experiencing the time of flowering and the colonial period. Their lives had the common things in various way. They were involved in Independence movement as the intellectual at that time. They also showed the various activities such as modern poem. sijo, and even Chinese poetry. In this thesis, 1 compared their sijo and considered them. Especially, I've researched the two divided parts focused on the tone shown in the sin one part was focused on the 'lover', and the other was on the 'life'. The summarise which I've researched is as followings. First, I've researched the sijo focused on the 'lover' They have the common things in having the presupposition of farewell to their 'lover'. But there were significant differences as followings. In the case of Yukdang, he expressed the passive tone based upon the despondency and the loss with the absence of his lover. Meanwhile, Manhae tended to reveal the resigned and negative tone based to his lyrical world. The contrasted tone in their poems is considered to have something to do with their realistic matters. There should also be several things to be discussed in the future.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.479-487
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• 2021

This study investigated the life history of the plum sawfly Monocellicampa pruni Wei, 1998 through field observations and laboratory experiments. M. pruni is a univoltine, complete metamorphosis species with five instars at the larval stage. Adults emerge from the soil around mid-March when Japanese plum trees are in their flowering period. The adults deposit one (rarely two) egg(s) under the epidermis layer of the calyx. After hatching, the larva soon burrows into the fruitlet, consumes the endocarp and remains inside the fruit within its four-time moulting transitions before reaching full growth. At maturity (in May), the larva leaves the infested fruitlet through an exit hole and burrows into the soil at a depth of 2 - 11 cm for spinning its cocoon and hibernates until the next spring as the larval form. Under laboratory conditions (T = 20℃; RH = 40 - 60%), the male lived for a slightly shorter period than the female, 6.03 ± 0.40 and 7.55 ± 0.45 days, respectively. A female could produce 30.29 ± 4.50 eggs in her lifespan. In the field, the duration of the egg was approximately 10 - 11 days and that of the larva was approximately 31 - 34 days.

• Korean Journal of Plant Taxonomy
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• v.51 no.3
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• pp.198-204
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• 2021

The plant known as "Hong-do-go-deul-ppae-gi" on Hongdo Island, belonging to the genus Crepidiastrum, has been recorded as C. ×nakaii, a hybrid between C. denticulatum and C. platyphyllum. During a survey of the flora of Hongdo Island, we reexamined the taxonomic identity of "Hong-do-go-deul-ppae-gi". The morphological traits, geographical distribution, and internal transcribed spacer (ITS) sequences of "Hong-do-go-deul-ppae-gi" were compared to related species within the genus. A morphological examination revealed that the plant was similar to C. lanceolatum in that it has subshrub stems with caudex stout, 8 inner involucrals bracts, and 10 to 12 florets. In contrast, similarities to C. denticulatum were observed in terms of radical leaves deciduous in the flowering period, cauline leaves membranous with acute serrations, and beaked achenes. Furthermore, C. denticulatum and C. lanceolatum grew on Hongdo Island and nearby areas, but C. platyphyllum was not distributed in these areas. The results of morphological and distributional investigations revealed that the Hongdo Island plant should be regarded as C. ×muratagenii, a hybrid between C. denticulatum and C. lanceolatum, not C. ×nakaii, which is a hybrid between C. denticulatum and C. platyphyllum. However, the resolution of the ITS sequences was insufficient such that we could not separate the examined species.

• Korean Journal of Breeding Science
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• v.49 no.3
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• pp.180-189
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• 2017

Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by global agricultural companies. Until now, GM crops have not been cultivated commercially in Korea. Commercialization of GM crops requires a compulsory assessment of environmental risk associated with the release of GM crops. This study was conducted to evaluate the frequency of pollen mediated gene flow from Bt transgenic rice (Agb0101) to japonica non-GM rice (Nakdongbyeo), indica non-GM rice (IR36), and weedy rice (R55). A total of 729,917, 596,318 and 230,635 seeds were collected from Nakdongbyeo, IR36, and R55, respectively, which were planted around Agb0101. Selection of the hybrids was determined by repeated spraying of herbicide and Cry1Ac1 immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using specific primer. The hybrids were found in all non-GM rice and out-crossing ranged from 0.0005% at IR36 to 0.0027% at Nakdongbyeo. All of hybrids were located within 1.2 m distance from the Agb0101 rice plot. The meteorological elements including rainfall and temperature during rice flowering time were found to be important factors to determine rice out-crossing rate. Consideration should be taken for many factors like the meteorological elements of field and physiological condition of crop to set up the safety management guideline to prevention of GM crops gene flow.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.294-297
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• 2010

A new intermediate parent 'Wonye 30001' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30001' could be used for hybrid seed production by crossing with pollen parent. The first cross was conducted in 2000. Male sterile plants were selected from 'Ginque' breeding lines and fertile plants were selected from YG-1-1. The male sterile line 'Wonye 30001' has circular bulb and bulb weight of 260 g. As mid-late maturing type, lodging date is around May 20. Plant height and stem diameter are 57 cm and 15.9 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 6 and 135cm, respectively. Flowering date of 'Wonye' is May 23 and is completely male sterile. 'Wonye 30001' is a promising male sterile line for hybrid bulb onion seed production.

• Korean Journal of Breeding Science
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• v.40 no.2
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• pp.196-200
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• 2008

A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Pinky' was bred from a cross between 'Rima' and 'Yen'. Progenies were investigated from 2004 to 2006 under shading culture in spring and retarding culture in autumn and the promising line was selected based on the performance of year-round production, white rust resistance and vase life. Pinky flowered around 20 October, and year-round production was possible by shading and lighting. Its flower was 3.1 cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. Days to flower from short-day start for 'Pinky' was about 47 in spring. 'Pinky' showed a vase life of 20.9 days in autumn. This cultivar was commercially registered in 2007. 'Pinky' would be suitable for culture under the greenhouse and rain shelter condition in Korea.

• Korean Journal of Breeding Science
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• v.40 no.2
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• pp.159-163
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• 2008

A New spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Water Fog' was bred by open pollination of 'Angaesoguk'. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Water Fog' was 24 October, but year-round production was possible by shading and lighting. It had flower diameter of 2.3 cm, 46.5 petals and 34.4 flowers per stem in autumn. The petal was narrow(0.4cm) and the color of petal was white with presence of green at flower center. Days to flower from short-day start for 'Water Fog' was about 48 in spring. 'Water Fog' showed the vase life of 22.6 days in autumn. This cultivar was registered for a commercialization in 2007. 'Water Fog' would be cultured under greenhouse and rain shelter condition in Korea.