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Effect of Variety and Stage of Maturity on Nutritive Value of Whole Crop Rice Silage for Ruminants: In situ Dry Matter and Nitrogen Degradability and Estimation of Metabolizable Energy and Metabolizable Protein

  • Islam, M.R.;Ishida, M.;Ando, S.;Nishida, T.;Yoshida, N.;Arakawa, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.11
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    • pp.1541-1552
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    • 2004
  • The effect of eight varieties of whole crop rice silage (WCRS) harvested at four stages of maturity were investigated for in situ DM and N degradability, ME and MP yield and content in an 8${\times}$4 factorial experiment. The varieties were Akichikara, Fukuhibiki, Habataki, Hamasari, Hokuriku 168, Kusanami, Tamakei 96 and Yumetoiro. Hamasari and Kusanami were forage varieties while all others were grain varieties. Forages were harvested on 10, 22, 34 and 45 days after flowering, ensiled and kept in airtight condition. Between 45 and 49 days after ensiling, silages opened, chopped and milled green to pass through 4 mm screen. Samples were incubated in the rumen of two Holstein steers for 0, 3, 6, 9, 12, 24, 48, 72 and 96 h over eight 4 d periods. Bags at 0 h were washed in a washing machine. Variety affected DM (p<0.001: except 'a+b', p<0.01) and N (p<0.001) degradability characteristics of WCRS. Stages of maturity also affected DM (p<0.001: except 'a+b', p<0.05; 'c', p<0.08) and N (p<0.01: except 'c', p<0.05) degradability characteristics of WCRS. Interactions between variety and stages of maturity occurred in all DM (p<0.001) and N (p<0.001) degradability characteristics except (p>0.05) for DM 'b', DM 'c', DM 'a+b' nd N 'c'. Effective DM degradability was higher in grain varieties than forage varieties and degradability increased with maturity. N availability decreased only slightly with maturity. Variety was the key factor for N degradability characteristics of WCRS since variety accounted for most of the total variation for degradability characteristics. Both ME and MP content and yield were higher (p<0.001) in grain varieties, and they increased (p<0.001) with the maturity. The results clearly demonstrated that the grain type varieties contained higher ME and MP content than forage varieties, and increase in maturity increases both ME and MP content of WCRS.

Intake, Digestibility In vivo, N Utilization and In sacco Dry Matter Degradability of Grass Silage Harvested at Three Stages of Maturity

  • Vranic, Marina;Knezevic, Mladen;Perculija, Goran;Bosnjak, Kresimir;Leto, Josip
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.2
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    • pp.225-231
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    • 2009
  • The objective of this experiment was to study the effects of grass maturity at harvest on the nutritive value of grass silage (GS) in relation to voluntary intake, digestibility, nitrogen (N) utilization and in sacco dry matter (DM) degradability. Silage was cut from a sward dominated by orchardgrass (Dactylis glomerata L.) at the late-vegetative (early-cut), internode elongation (mediumcut) and flowering (late-cut) stages of growth. The DM yield at harvest was the lowest for early-cut silage (5.4 t/ha) and increased to 6.5 and 7.0 t/ha for the medium and late-cut silage respectively. As the crop matured, the crude protein (CP) concentration decreased significantly (p<0.05) and there was a marked increase in acid detergent fiber (ADF) concentration (p<0.001). The three different silages were offered to four 18-month old Charolais wether sheep to measure the voluntary intake, in vivo digestibility and N retention over four 21-day periods in an incomplete changeover design. Silage degradability characteristics were determined using four fistulated sheep to measure DM degradability over 3, 6, 12, 24, 48 and 72 h. There was a linear decrease in the voluntary intake of silage fresh matter, DM, organic matter (OM) and neutral detergent fiber (NDF), digestibility of DM, OM, NDF, ADF and CP, and digestibility of OM in DM (Dvalue) ($P_L<0.01$) as harvesting of grass was delayed. Nitrogen intake, N output in urine, N output in faeces and N balance also linearly decreased ($P_L<0.01$) with postponed harvesting of grass for silage. DM degradability and effective degradability (ED) significantly decreased with increasing maturity of grass at harvest. The results suggest that harvesting date has a significant influence on the nutritive value of GS in terms of intake, digestibility, N balance and in sacco degradability in the rumen. It was concluded that early harvest GS ensured higher intake, digestibility, N intake and DM degradability in comparison with the medium and the late cut GS as a result of improved rumen N efficiency and utilization probably due to a better balance of available energy and protein.

Studies on the Storage of Apple -(V) On the Harvesting Date of Spur Golden Delicious Apple- (사과저장(貯藏)에 관(關)한 연구(硏究) -(V) Spur Golden Delicious의 수확기(收穫期)에 관(關)하여-)

  • Park, Nou-Poung;Kim, Yun-Jin;Rhee, Chong-Ouk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.5 no.1
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    • pp.19-24
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    • 1976
  • A determination of proper maturity is a significant factor for a good quality and storage. This study is aimed at a determination of optimum harvest date related to a decisive measuring of maturity in Spur Golden Delicious. In this case, respiratory status and other characteristics are taken as a indicator for maturing determination. The results are as follows. 1. Respiratory rate has been decreased through a fruit development and come to its minimized point on Sep. 25. at last. On the contrary its tendency has been increased after Sep. 25. It is also estimated that the former is pre-climacteric, the latter climacteric rise based on dimacteric minimum Sep. 25. 2. A flesh hardness has maintained comparably high value until Sep. 18. But in the point of climacteric minimum, Sep. 25, curve of flesh firmness has rapidly been broken down, and after Sep. 25, tendency has slowly decreased at the sucessive date. 3. Pectin content, optimum acidity are remarkably decreased after Sep. 25, Oct. 2 respectively. 4. When water soluble sugar content shows to be swung from climacteric minimum, any resonable tendency are not found. 5. In this experiment, optimum harvest time is found by Oct. 2, in time of 145 days after flowering.

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Effects of Minimizing the Heating Space on Energy Saving and Hot Pepper(Capsicum annuum L.) Growth in the Plastic Greenhouse (온실 난방공간 최소화가 에너지 절감 및 고추 생육에 미치는 영향)

  • Tae Young Kim;Young Hoe Woo;Ill Hwan Cho;Young Sam Kwon;Si Young Lee;Han Ik Jang
    • Journal of Bio-Environment Control
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    • v.10 no.4
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    • pp.213-218
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    • 2001
  • In 2000, domestic protected cultivation area was about 52,189 ha including 13,621 ha of heating greenhouses. Recently, heating cost accounts for 25 to 30% of total production cost which has been increased due to the rise of oil price, while the heating cost was about 15% in other advanced countries. To reduce the heating energy cost, the study of minimizing the heating space of greenhouse have been conducted from 1998 to 1999. The system was developed to control the heating space according to crop growth by moving horizontal curtain up and down. Installation of the heating space-control curtain in greenhouse decreased heating capacity to 264 m$^3$compared to 661.5 m$^3$in the traditional curtain, and consumpted fuel was saved about 56% point in semiforcing culture and 28% point in retarding culture of pepper. In addition, uniform distribution of air temperature and relative humidity in greenhouse environment resulted in earlier flowering and higher yields in hot pepper.

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A Study on the Original Planting Form and Management of Vegetation of the Scenic site Nr. 60 Chungam Pavilion and Seokchun Valley in Bonghwa (명승 제60호 봉화 청암정과 석천계곡의 식재 원형 및 식생 관리 방안에 관한 연구)

  • Yee, Sun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.32 no.4
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    • pp.73-84
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    • 2014
  • This research has conducted historical data analysis and on-site investigation on Chungam Pavilion and Seokchun Valley in Bonghwa. Based on the results, the original planting form was identified, and the vegetation management were drawn up. The results of this study can be summarized as follows. First of all, The Book of Seokchun states that there used to be forest trees such as pine tree, saw-leaf zelkova and maple tree, and flowering trees such as peony, royal azalea, rose, chrysanthemum, and lotus, planted around Chungamjeong. Around Seokchunjeongsa, there used to be boxwood, bamboo, maple, peach, gooseberry and peony planted in the area. Through this discovery it can be realized that the vicinity of Chungam Pavilion and Seokchunjeongsa provided not only the grand aesthetics of four clear seasons, but also planting landscapes with various symbolistic significance and meanings. The description of the planting situation in the historical records offers a useful reference for future vegetation management in Chungam Pavilion, Seokchunjeongsa and surrounding valleys. Furthermore, it holds critical value in the identification of planting pattern in late Chosun dynasty. Second, the planting landscape in and around Chungam Pavilion and Seokchunjeongsa will have to be maintained as described in the historical records The Book of Seokchun. Also, the once famous colony of pine trees in the area is gradually reducing in its scale, which calls for active management measures such as elimination of broadleaf trees.

Studies on the cultivation preceding crop of paddy-field of green vegetable(soybean for cook with rice) by short-day treatment (혼반용 대두(풋콩)의 답전작재배에 관한 연구 (제3보))

  • Ki-Jun Kim;Jong-Sun Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.31-36
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    • 1972
  • These studies were carried out to find out the proper plant spacing and the optimum amount of nitrogen for maximum yield, on the occasion of early planting with creen vegetable (soybean for cook with rice) at the low temperature in early spring. The plant spacing were applied in 5 levels (50cm$\times$5cm, 50cm$\times$10cm, 40cm$\times$10cm, 30cm$\times$10cm and 30cm$\times$20cm) and the amount of nitrogen were applied in 4 levels (non, standard, twice and triple-amount), The triple super phosphate and potassium chloride were applied only in standard amount. The promotion of flowering was practised by the short-day treatment for 10 days (11 hr. a day). The variety examined was the early maturing one, HOKKAI # 1. and the results are as follow. 1. The plant spacing for maximum yield by the promotion of branch, pod and garin per a plant recognized the fact that there were 2 levels (50cm$\times$10cm and 30cm$\times$20cm planting method) and the maximum yield by the promotion of pod and grain per area showed the fact that there were 2 levels (50cm$\times$5cm and 30cm$\times$10cm planting method) in narrow planting method. 2. The optimum amount of nitrogen applied for maximum yield of pod and grain per area recognized w hat was sufficient as standard amount.

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Studies on Rapid Advancement of Generations for a Breeding Efficiency Promotion of Rape Oil Improvement IV. Effect of Green Plant Vernalization on Shortening the Growing Period of Summer Cultured Rape in Field (유채 성분육종 효율을 증진키 위한 세대단축 기술개발에 관한 연구 제4보 Winter형 유채의 유묘기 록체 저온감응이 하계고온 포장재배에 미치는 효과)

  • Lee, J.I.;Kwon, B.S.;Kim, W.C.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.1
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    • pp.133-138
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    • 1983
  • In Korea, Brassica species planted in summer remain in rosette and do not flower. Seed production of vernalized rape seedlings grown in summer was evaluated to establish method of generation shortening and genetically pure seed production without isolation of seed fields. When vernalized rape seedlings of different maturity groups were transplanted in summer, all plants bolted and flowered, and average days from sowing to bolting and flowering was 37 and 58. respectively. It took 85days for early maturity group, 88 days for medium maturity group, and 108 days for late maturity group to mature. Seed yield of vernalized plants grown in summer was 8.8 gr per plant and 53kg per l0a which was 23% of seed yield of rape planted in autumn. However, enough seeds were produced to test oil composition and cake and to plant for a next generation. If seedlings are raised, they can be transplanted in more than 1, 000 times of original seed field, indicating that genetically pure seed can be produced without caging seed fields with nets and isolation of seed fields since other Brassica species do not flower in summer.

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Effects of Transplanting Dates on Major Agronomic Characteristics and Stem Yield in Mat Rush(Juncus effusus L. var. decipiens, Bush) (골풀 정식시기에 따른 주요형질 및 수량변이)

  • Kwon, Byung-Sun;Lee, Jung-Il;Son, Eung-Ryong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.3
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    • pp.317-322
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    • 1987
  • In order to find out the optimum transplanting date of mat rush, this study was investigated ecological characters, yield components and yield of three varieties (Okayama#2, Sajanami and Sedo #4) at 5 transplanting dates(October 25, November 5, November 15, November 25 and March 5). As transplanting date was delayed, the flowering date and maturing date was delayed also and stem length was longest in October 25 transplanting but it grew short in the late transplanting. Number of stems per plant was increased most in October 25 transplanting, that is, increased to 143 in okayama#2, 138 in Sedo#4 and 135 in Sajanami, and it was decreased in the late transplanting. Fresh stem yield and dry stem yield were heaviest in October 25 transplanting, and it was light in the late transplanting. Judging from the results reported above, in optimum transplanting date of mat rush seemed to be before the October 25.

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The Endophytic Bacteria Bacillus velezensis Lle-9, Isolated from Lilium leucanthum, Harbors Antifungal Activity and Plant Growth-Promoting Effects

  • Khan, Mohammad Sayyar;Gao, Junlian;Chen, Xuqing;Zhang, Mingfang;Yang, Fengping;Du, Yunpeng;Moe, The Su;Munir, Iqbal;Xue, Jing;Zhang, Xiuhai
    • Journal of Microbiology and Biotechnology
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    • v.30 no.5
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    • pp.668-680
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    • 2020
  • Bacillus velezensis is an important plant growth-promoting rhizobacterium with immense potential in agriculture development. In the present study, Bacillus velezensis Lle-9 was isolated from the bulbs of Lilium leucanthum. The isolated strain showed antifungal activities against plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea and Fusarium fujikuroi. The highest percentage of growth inhibition i.e., 68.56±2.35% was observed against Fusarium oxysporum followed by 63.12 ± 2.83%, 61.67 ± 3.39% and 55.82 ± 2.76% against Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi, respectively. The ethyl acetate fraction revealed a number of bioactive compounds and several were identified as antimicrobial agents such as diketopiperazines, cyclo-peptides, linear peptides, latrunculin A, 5α-hydroxy-6-ketocholesterol, (R)-S-lactoylglutathione, triamterene, rubiadin, moxifloxacin, 9-hydroxy-5Z,7E,11Z,14Z-eicosatetraenoic acid, D-erythro-C18-Sphingosine, citrinin, and 2-arachidonoyllysophosphatidylcholine. The presence of these antimicrobial compounds in the bacterial culture might have contributed to the antifungal activities of the isolated B. velezensis Lle-9. The strain showed plant growth-promoting traits such as production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, and nitrogen fixation and phosphate solubilization. IAA production was accelerated with application of exogenous tryptophan concentrations in the medium. Further, the lily plants upon inoculation with Lle-9 exhibited improved vegetative growth, more flowering shoots and longer roots than control plants under greenhouse condition. The isolated B. velezensis strain Lle-9 possessed broad-spectrum antifungal activities and multiple plant growth-promoting traits and thus may play an important role in promoting sustainable agriculture. This strain could be developed and applied in field experiments in order to promote plant growth and control disease pathogens.

Analysis of Protein Function and Comparison on Expression of Protein in Taekwang During Maturation using Proteomic Techniques (Proteomics를 이용한 등숙기 차이에 따른 콩 종실 저장단백질 발현양상 비교 분석)

  • Cho, Seong-Woo;Kim, Tae-Sun;Kwon, Soo-Jeong;Roy, Swapan Kumar;Lee, Chul-Won;Kim, Hong-Sig;Woo, Sun-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.1
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    • pp.41-46
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    • 2015
  • In the present study, different expression of protein from Taekwang was revealed by 2-DE, and expressions of protein on each week after flowering was investigated. After analysis of expression of protein, MALDI-TOF was executed to identify expected protein function. Results revealed that there were three patterns of expression of protein during the maturing. The first pattern was that proteins were gradually expressed as up-regulation from 1 week to 6 week. The second pattern was that proteins were expressed gradually from 1 week to 5 week and then it started down-regulation in 6 week. The last pattern was that proteins were gradually as up-regulation from 1 week to 3 week and then down-regulation until 6 week. This phenomenon suggests that young stage has more protein related to correspondence mechanism against disease and growth and then maturing stage has more expression of protein related to storage protein. In MALDI-TOF analysis, p24 oleosin isoform A protein was identified that relates oleosin which is synthetic product in oil body. This protein spot increased gradually until 5 week and then decreased after 5 week. It explained that the protein is active until maturing stage to protect oil in seed and then its activity has gradually degraded. This result may be expected that a protein, related to growth of a seed has increased until maturing and then a seed fills up with a storage protein.