• Korean Journal of Ichthyology
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• v.30 no.2
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• pp.71-74
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• 2018

The karyotype analysis of an endangered freshwater fish, Microphysogobio koreensis, was performed to obtain basic data for phylogenetic information. To carry out our study, 4 specimens were collected in Seomjingang River and Nakdongang River and its kidney was treated by flame-drying method. The chromosome number of this species demonstrated 50 diploid chromosomes, with two populations of M. koreensis not significantly different. The karyotype revealed 2n=26m+24sm, consisting of 26 metacentric (m) and 24 submetacentric (sm) chromosomes with the total fundamental arm number determined as FN=100. Total arm length and arm ratio of the chromosomes were $1.44{\sim}2.68{\mu}m$ and 1.27~2.27, respectively. The karyotype of M. koreensis was first reported in this study.

• Korean Journal of Plant Taxonomy
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• v.43 no.2
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• pp.139-145
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• 2013

This study was carried out to determine the karyotype and chromosomal localizations of 45S and 5S rDNAs using FISH in Astragalus koraiensis. The somatic metaphase chromosome number of this species was 2n = 16 with basic chromosome number of x = 8. The karyotype of A. koraiensis was consisted of six pairs of median region chromosomes(chromosome 1, 3, 4, 5, 6, 8) and two pairs of submedian chromosomes(chromosome 2, 7). Based on the FISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 5. Whereas, two pair of 5S sites were detected on the short arm of chromosome 4 and centromeric region of chromosome 7, respectively. These are quite different patterns from A. membranaceus, A. membranaceus var. alpinus, and A. mongholicus. Although A. koraiensis is considered as Korean endemic species, therefore, it should be conducted out comparative FISH study with A. sikokianus and A. bhotanensis which are very similar to A. koraiensis morphologically.

• Korean Journal of Plant Taxonomy
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• v.38 no.2
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• pp.151-162
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• 2008

Chromosome analysis using karyotyping and bicolor FISH were carried out in Pimpinella hallaisanensis which is one of the endemic plants in Jeju island of Korea. The somatic methaphase chromosomes number of this plant was 2n=2x=22 and the size of this chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentrics (chromosomes 1 and 2), four pairs of submetacentrics (chromosomes 3, 4, 6 and 8) and five pairs of subtelocentrics (chromosomes 5, 7, 9, 10 and 11). Using bicolor FISH, three pairs of 5S and four pairs of 45S rDNA loci were observed. Two pairs of 5S rDNA signals were detected on the end of the long arm of chromosome 4 and one pair of them were observed between long arm end and centromere. Another 45S rDNA signals were detected on the end of short arm of chromosome 4, 6, 10 and 11, respectively. Hence, the chromosome number reexamined using both conventional staining and FISH methods was different from previous report.

• Korean Journal of Ichthyology
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• v.20 no.1
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• pp.61-65
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• 2008

The karyotype analysis of Kichulchoia brevifasciata was carried out, known as an end-angered small endemic cobitid fish of Korea. Five females and one male were collected from Geum-san-myeon, Goheung-gun, Jeollanam-do, Korea and its gill, kidney and digestive tract tissues were treated by flame-drying technique. The karyotype was found to be 2n= 48 from 33 cells; 16 meta-submetacentric and 32 subtelo-telocentric chromosomes; 64 FN (fundamental number). This karyotype showed a remarkably different karyotype formula from Niwaella multifasciata which is closely related to this species.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.65-68
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• 2008

Molecular cytogenetics allows the identification of unknown chromosome rearrangements, which is clinically useful in patients with mental retardation and/or development delay. We report on a 31-year-old woman with severe mental retardation, behavior development delay, and verbal performance delay. Conventional cytogenetic analysis showed a 46,XX,add(8)(p23.3) karyotype. To determine the origin of this unbalanced translocation, we performed array CGH and subtelomeric FISH. The results showed that the distal region of chromosome 8p was added to the terminal of chromosome 13q. This was confirmed the final result of 46,XX,der(8)t(8:13)(p23.3;q32.1)dn.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.47-54
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• 2008

Purpose : Fluorescence in situ hybridization (FISH) on uncultured amniotic fluid cells offers the opportunity for rapid screening of aneuploidies and has become an integral part of the current practice in many clinical cytogenetics laboratories. Here, we retrospectively analyzed the results of interphase FISH in 943 amniotic fluid samples and assessed the efficiency of FISH for rapid detection of aneuploidies. Methods : Interphase FISH for chromosome 13, 18, and 21 was performed in 943 consecutive amniotic fluid samples for rapid diagnosis of aneuploidies referred from 2004 to 2006. Karyotypes from standard cytogenetic analysis were compared to the FISH results. Results : A total of 45 chromosomal rearrangements (4.8%) were found after conventional cytogenetic analysis of the 943 amniotic fluid. After exclusion of known familiar chromosomal rearrangements and inversions (2.1%, 20/943), 2.7% (25/943) were found to have chromosomal abnormalities. Of this group, 0.7% (6/943) were chromosomal abnormalities not detectable by FISH and 2.0% (19/943) were numerical abnormalities detectable by FISH. All 14 cases of Down syndrome (Classic type, 13 cases; Robertsonian type, 1 case) and 5 cases of trisomy 18 were diagnosed and detected by FISH and there were no false-positive or -negative results (specificity and sensitivity=100%). Conclusion : The present study demonstrates that FISH can provide a rapid and sensitive clinical method for prenatal identification of chromosome aneuploidies. However, careful genetic counseling is essential to explain the limitations of FISH, including the inability to detect all chromosomal abnormalities and the possibilities of uninformative or false-negative results in some cases.

• The Korean Journal of Zoology
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• v.34 no.1
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• pp.54-58
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• 1991

감돌고기속 어류에는 2종이 알려져 있으며 이들은 모두 한국고유어종이다. 감돌고기 Pseudopugtungia nigra의 핵형분석 결과 diploid chromosome number는 50이었으며, 7쌍의 metacentric, 18쌍의 $_{submeta}$telocentric chromosome으로 구성되어져 잇었다. 가는 돌고기 P. tenuicorpus의 2N은 50이었으며 10쌍의 metacentric, 15쌍의 $_{submeta}$telocentric chromosome으로 구성되어져 있었다.

• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.193-198
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• 2009

Chromosome analysis using karyotyping and bicolor FISH were carried out for two Maackia species (M. fauriei and M. amurensis) found in Korea. The somatic metaphase chromosome number was 2n = 2x = 18 in both, and the size of these chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentric (chromosomes 1 and 7), four pairs of submetacentrics (chromosomes 4, 6, 8 and 9) and three pairs of subtelocentrics (chromosomes 2, 3 and 5) in M. fauriei but, chromosomes 4 (subtelocentric) and 7 (submetacentric) of M. amurensis have different morphology. Using bicolor FISH, a pair of 45S rDNA loci were observed for both M. fauriei and M. amurensis, but the number and site of the 5S rDNA signal were different in the two species. M. fauriei has two pairs of 5S signals on chromosomes 7 and 8 but, M. amurensis has four paris on chromosomes 3, 4, 7 and 7. Hence, the 5S rDNA is a useful FISH for Maackia species.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.48-51
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• 2005

Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged $1.95{\sim}3.50\;{\mu}m$. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.

• Korean Journal of Ichthyology
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• v.28 no.3
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• pp.175-178
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• 2016

The karyotype analysis of Liobagrus somjinensis, an endemic Korean freshwater fish with restricted waters and a new species of torrent catfish was carried out from nine females and eight males of Somjingang River, Sikjeong-dong, Namwon-si, Jeollabuk-do, Korea. The diploid number of chromosomes was 42, and its karyotype was composed of 28 metacentrics and 14 submetacentrics; 84 FN (fundamental number). Polyploidy and sex dimorphism were not observed in the present species. The chromosome number of L. somjinensis was the same as its congeners, but there was a difference in karyotypes.