• Title/Summary/Keyword: FIM

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Optimal culture conditions for production of Escherichia coli Adhesin protein coupled to Escherichia coli Heat Labile Enterotoxin A2B in Escherichia coli TB1.

  • Lee, Yong-Hwa;Rhee, Dong-Kwon;Pyo, Suhk-Neung
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.226.2-226.2
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    • 2003
  • The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause of urinary tract infection. To produce a possible vaccine antigen against urinary tract infection, the fimH gene was genetically linked to the Itxa2b gene, which was then cloned into the pMAL -p2E expression vector. The chimaeric construction of pMALfimH/Itxa2b was transformed into Escherichia coli TB1 and its N-terminal amino acid sequence was analyzed. (omitted)

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Expression and Characterization of Escherichia coli Adhesin Protein Linked to Cholera Toxin A2/B Subunits in Escherichia coli

  • Lee, Young-Hwa;Ryu, Dong-Kyun;Rhee, Dong-Kwon;Pyo, Suhk-Neung
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.309.2-309.2
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    • 2002
  • The FimH subunit of type l-fimbriated Escherichia coli has been determined as a major cause of urinary tract infection. To produce a possible vaccine antigen against urinary tract infection, the fimH gene was genetically coupled to the ctxa2b gene, which was then cloned into pMAL -p2E expression vector. The chimaeric construction of pMALfimH/ctxa2b was transformed into Escherichia coli TB1 and its N-terminal amino acid sequence was analyzed. (omitted)

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Effects of Client-centered Mental Practice on Stroke Patients' Activities of Daily Living and Quality of Life (클라이언트 중심 상상훈련이 뇌졸중 환자의 일상생활 수행과 삶의 질에 미치는 영향)

  • Park, Ju-Hyung;Yoo, Chan-Uk
    • The Journal of the Korea Contents Association
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    • v.17 no.11
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    • pp.533-540
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    • 2017
  • The aim of this study was to investigate the effects of Client-centered mental practice on activities of daily living and quality of life with stroke. For 16 persons with stroke, we executed Client-centered mental practice for 4 weeks, five times per week, and 30 minutes each time. Before and after the Client-centered mental practice, the patients were tested with Functional Independence Measure(FIM) and Stroke specific quality of life(SS-QOL). After the Client-centered mental practice, the subjects showed significant changes in the score of FIM and SS-QOL(p<0.05). The results of this study show that Client-centered mental practice for persons with stroke is a useful therapeutic approach by enhancing through improving their activities of daily living and quality of life.

The Correlations between the Balance Test, functional movement, Visual Perception Test and Functional Independent Measure in Stroke Patients (뇌졸중 환자의 균형, 기능적 보행, 시지각, 일상생활 평가도구의 상관성)

  • Lee, Dong-Jin;Kim, Seong-Yeol;Song, Chang-Ho
    • The Journal of Korean Physical Therapy
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    • v.21 no.2
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    • pp.39-45
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    • 2009
  • Purpose: The purpose of this study was to determine correlations between the Berg Balance Scale (BBS), Functional Reach Test (FRT), Timed Up & Go (TUG), Motor-Free Visual Perception Reach Test Vertical format (MVPT-V), Functional Independence Measure (FIM). Methods: The subjects used in this study were 35 stroke patients from Cheongju ST. Mary's hospital. Balance was measured by BBS, FRT. Functional mobility was measured by TUG. Visual perception was measured by MVPT-V. FIM was used to evaluate the activities of daily living. Data was analyzed using pearson product correlation. The TUG and MVPT-V index were analyzed by linear regression. Results: There was a statistically significant difference between FRT and BBS (r=0.89, p<0.01), FIM (r=0.74, p<0.05), MVPT-V (r=0.40, p<0.05), and TUG (r=-0.36, p<0.05). There was significant statistical differences between TUG and MVPT-V (r=-0.64, p<.01). However, statistically significant differences were observed between BBS and FIM (r=0.79, p<0.01). The visual close item of the MVPT-V showed the strongest variance in predicting TUG. Conclusion: The use of both quantitative and qualitive scales was shown to be a good measuring instrument for the classification of general clinical performances of stroke patients. In particular, the results suggest that the visual perception test may be able to predict functional locomotion in stroke patients.

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Effects on Improvement of Activities of Daily Living Through Short-Term Home Visiting Physical Therapy (단기 가정방문물리치료 시행이 일상생활동작의 개선에 미치는 효과)

  • An, Duk-Hyun
    • Physical Therapy Korea
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    • v.14 no.2
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    • pp.53-60
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    • 2007
  • The objective of this study was to discover the effects of the short-term home visiting physical therapy program involving patients with some chronic brain disorders at Gimhae City, Kyongnam. Recovery of activities of daily living (ADL) is a very important factor of rehabilitative procedures, and Functional Independence Measure (FIM) is a useful standard of evaluation for it. The FIM is widely used in brain disorder research because it measures real functional activities of daily living. We applied the physical therapy exercise program twice per week (10~15 times repeatedly); a warm-up and cool down exercise performed every ten minutes by active & active-assistive ROM and stretching exercises. Main exercises were composed of getting up & laying down in bed, standing training, walking exercise in the room, and window or wall sliding exercise using affected upper limbs for a total duration of 30 minutes. We collected the data from 20 patients with chronic brain disorders at his/her home and analyzed by means of SPSS/PC+ program (Ver. 10.0). After the six week long physical therapy exercise program, the average was $56.10{\pm}22.59$ point compared with initial $50.55{\pm}19.12$ point by FIM, improved functional ADL ability about 5.55 point, and these changed scores were statistically significant (p=.000). We also studied another factor regarding patient's satisfaction. The majority of subjects (10 people) rated the program with the maximum score of ten points (50.0%), and three people rated it a seven point program (15.0%), the other two subjects gave a rating of nine and eight points (10.0%). Because the program was effective at improving the physical ADL ability and satisfaction of each subject, we suggest continual development and implementation of a home visiting physical therapy program. Further study should involve a longer period of observation with a larger population that is involved in an individually designed home physical therapy program.

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Development of Reverse Transcriptase-Polymerase Chain Reaction of fimA Gene to Detect Viable Salmonella in Milk (우유 내 활력있는 Salmonella를 검출하기 위한 fimA 유전자의 역전사중합효소 연쇄반응의 개발)

  • Choi, S.H.;Lee, S.B.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.841-848
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    • 2004
  • Rapid detection of viable Salmonella in pasteurized milk is important to protect public health from food poisoning. Reverse transcriptase-polymerase chain reaction(RT-PCR) is recognized as a molecular genetical method to differentiate between live and dead bacteria The RT-PCR in this study was designed to detect specifically viable Salmonella in milk by using the primers whose nucleotide sequences were determined based on fimA gene which encodes the submit of type 1 fimbriae. Treatment of RNA preparation with RNase-free DNase was adequate enough to destroy DNA, which may otherwise be amplified in the RT PCR Seven strains of Salmonella were detected in the RT-PCR but Escherichia coli, Shigella sonnei, Citrobacter freundii, and Klebsiella pneumoniae were not. $10^7/ml$ and $10^6/ml$ of dead Salmonella which were heat-treated in milk were detectable by using the RT-PCR but $10^5{\sim}10/ml$ of the dead bacteria were not. The sensitivity of the RT-PCR in detecting viable Salmonella was 100 cells/ml.

A Study on the Performance of Multicast Transmission Protocol using FEC Method and Local Recovery Method based on Receiver in Mobile Host (이동 호스트에서 FEC기법과 수신자 기반 지역복극 방식의 멀티캐스트 전송 프로토콜 연구)

  • 김회옥;위승정;이웅기
    • Journal of Korea Multimedia Society
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    • v.5 no.1
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    • pp.68-76
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    • 2002
  • Multicast in mobile host has the problem of hast mobility, multicast decision, triangle routing, tunnel convergence, implosion of retransmission, and bandwidth waste. In particular, the bandwidth waste in radio is a definite factor that decreases transmission rate. To solve the problems, this paper proposes a new multicast transmission protocol called FIM(Forward Error Correction Integrated Multicast), which supports reliable packet recovery mechanism by integrating If Mobility Support for the host mobility, IGMP(Interned Group Management Protocol) for the group management, and DVMRP(Distance Vector Multicast Routing Protocol) for the multicast routing, and it also uses FEC and the local recovery method based on receiver. The performance measurement is performed by dividing the losses into the homogeneous independent loss, the heterogeneous independent loss, and the shared source link loss model.. The result shows that the performances improves in proportion to the size of local areal group when the size of transmission group exceeds designated size. This indicates FIM is effective in the environment where there are much of data and many receivers in the mobile host.

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Development of Integrated Flood Analysis Program for Standardization of Disaster Map (재해지도 작성 표준화를 위한 내·외수 통합 침수해석 프로그램(i-FIM)의 개발)

  • Lee, Jae Yeong;Keum, Ho Jun;Kim, Beom Jin;Cha, Young Ryong;Han, Kun Yeun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2018.05a
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    • pp.278-278
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    • 2018
  • 현재 우리나라에서는 행정안전부의 풍수해저감종합계획, 사전재해영향성검토협의, 재해위험지구개선사업 등에 해외에서 개발된 상용프로그램이 사용돼 접근성 저하로 인해 지자체 방재담당자의 실무나 대학에서 연구용으로 다루기에는 한계가 있다. 이에 본 연구에서는 내수침수, 외수침수, 2차원 침수해석으로 구성하여 GUI 기능을 강화한 통합침수재해지도 작성시스템(i-FIM, Integrated Flood Inundation Modeling system)을 개발하여 입력자료의 구성 및 매개변수의 수정이 용이하게 함으로써 하수관망 등에 부분적인 설계 변경이 있는 경우 지자체 방재담당자가 간단한 작업을 통해 침수영향 변화를 쉽게 파악할 수 있도록 하였다. 또한, 상세한 지형의 반영이 필요한 도시지역의 2차원 침수해석의 경우 계산격자 망의 크기가 작아질수록 소요되는 계산시간이 기하급수적으로 증가하는 한계가 있어 i-FIM에서는 계산격자를 $2{\times}2$, $3{\times}3$, $5{\times}5$ Subgrid 형태의 격자를 적용하고, 병렬프로그래밍과 계산시간조정 기능을 추가하여 2차원 침수해석 모형의 계산 속도를 향상시켰다. 이를 실무에 적용하기 앞서 2006년 집중호우로 인해 안성시에서 발생한 제방 붕괴사상, 2016년 태풍 차바로 인해 울산시에서 발생한 제방 월류 사상을 통해 침수흔적도와 비교하여 검증을 실시하였다. i-FIM에서 최종적인 2차원 침수해석 결과는 2017년에 개정된 '재해지도 작성 기준 등에 관한 지침'의 침수심 등급 구분의 색채 설정에 따라 각 격자별 침수심을 표출함으로써 표준화된 재해지도 작성이 가능하도록 하였다. 또한, 포털사이트의 지도 및 위성지도에 표출함으로써 침수 위험이 발생할 수 있는 지역의 현재 이용 용도를 파악하여 침수재해에 대한 상세한 대책을 마련할 수 있을 것으로 판단된다.

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The Correlation Between Early Clinical State and Functional Outcome in Acute Stroke Patients (급성기 뇌졸증 환자의 상태와 기능회복도와의 상관관계)

  • Choi, Eun-Jung;Lee, Won-Chul
    • The Journal of Dong Guk Oriental Medicine
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    • v.6 no.2
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    • pp.167-190
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    • 1998
  • Nowadays there were two tendencies of studies about prognostic factors in stroke. One way was to define prognostic factors according to the radiological features. And the other way was to define according to the mental state, recognition, perception, motors, language, urinary&bowel incontinence etc.. The former could be objectively investigated, while the latter was difficult. The purpose of this study was to determine which variables would be predictors of stroke and which factors would be affect predictions most. The subjects of this study were 32 patients who were admitted to the Dept. of Internal Medicine, Dongguk Univ. College of Oriental Medicine whthin 48 hours from attack, Medical records were reviewed FIM, CNS, NIH stroke scale. We compared each sub-items of FIM, CNS, NIH stroke scale about mental state, recognition, perception, motors, language, urinary&bowel incontinence with MBI score at 4 weeks from admission. Also, we analyzed the correlations of sub-items and groups which devided into 5 according to independence of MBI score. And we found out the most influent factors with multiple regression analysis. The major results were as follows; 1. In mean of MBI score at 4 weeks of each groups devided low, middle, high score at mental state, recognition, perception, motors, language, urinary&bowel incontinence items, there were statistical differences in all items. 2. The mental state and lim ataxia sub-items had no significant correlations with groups divided according to independence of MBI score. All the other items were significantly correlated. 3. The most influent factors was recognition. The second was sensory and the third was bowel incontinence. 4. The most influent scales was FIM, and the second was CNS, and NlH had no statistical significancy.

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Determination of optimal accelerometer locations using modal sensitivity for identifying a structure

  • Kwon, Soon-Jung;Woo, Sungkwon;Shin, Soobong
    • Smart Structures and Systems
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    • v.4 no.5
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    • pp.629-640
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    • 2008
  • A new algorithm is proposed to determine optimal accelerometer locations (OAL) when a structure is identified by frequency domain system identification (SI) method. As a result, a guideline is presented for selecting OAL which can reflect modal response of a structure properly. The guideline is to provide a minimum number of necessary accelerometers with the variation in the number of measurable target modes. To determine OAL for SI applications effectively, the modal sensitivity effective independence distribution vector (MS-EIDV) is developed with the likelihood function of measurements. By maximizing the likelihood of the occurrence of the measurements relative to the predictions, Fisher Information Matrix (FIM) is derived as a function of mode shape sensitivity. This paper also proposes a statistical approach in determining the structural parameters with a presumed parameter error which reflects the epistemic paradox between the determination of OAL and the application of a SI scheme. Numerical simulations have been carried out to examine the proposed OAL algorithm. A two-span multi-girder bridge and a two-span truss bridge were used for the simulation studies. To overcome a rank deficiency frequently occurred in inverting a FIM, the singular value decomposition scheme has been applied.