• Biomolecules & Therapeutics
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• 제29권4호
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• pp.427-433
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• 2021

Free fatty acid receptor 2 (FFA2, also known as GPR43), a G-protein-coupled receptor, has been known to recognize short-chain fatty acids and regulate inflammatory responses. FFA2 gene deficiency exacerbated disease states in several models of inflammatory conditions including asthma. However, in vivo efficacy of FFA2 agonists has not been tested in allergic asthma. Thus, we investigated effect of 4-chloro-α-(1-methylethyl)-N-2-thiazoylylbenzeneacetanilide (4-CMTB), a FFA2 agonist, on antigen-induced degranulation in RBL-2H3 cells and ovalbumin-induced allergic asthma in BALB/c mice. Treatment of 4-CMTB inhibited the antigen-induced degranulation concentration-dependently. Administration of 4-CMTB decreased the immune cell numbers in the bronchoalveolar lavage fluid and suppressed the expression of inflammatory Th2 cytokines (IL-4, IL-5, and IL-13) in the lung tissues. Histological studies revealed that 4-CMTB suppressed mucin production and inflammation in the lungs. Thus, results proved that FFA2 functions to suppress allergic asthma, suggesting 4-CMTB activation of FFA2 as a therapeutic tool for allergic asthma.

• Biomolecules & Therapeutics
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• 제28권3호
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• pp.267-271
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• 2020

Gut microbiota produce dietary metabolites such as short-chain fatty acids, which exhibit anti-inflammatory effects. Free fatty acid receptor 2 (FFA2, formerly known as GPR43) is a specific receptor for short-chain fatty acids, such as acetate that regulates inflammatory responses. However, the therapeutic potential of FFA2 agonists for treatment of atopic dermatitis has not been investigated. We investigated the efficacy of the FFA2 agonist, 4-chloro-α-(1-methylethyl)-N-2-thiazoylylbenzeneacetanilide (4-CMTB), for treatment of atopic dermatitis induced by 2,4-dinitrochlorobenzene (DNCB). Long-term application of DNCB to the ears of mice resulted in significantly increased IgE in the serum, and induced atopic dermatitis-like skin lesions, characterized by mast cell accumulation and skin tissue hypertrophy. Treatment with 4-CMTB (10 mg/kg, i.p.) significantly suppressed DNCB-induced changes in IgE levels, ear skin hypertrophy, and mast cell accumulation. Treatment with 4-CMTB reduced DNCB-induced increases in Th2 cytokine (IL-4 and IL-13) levels in the ears, but did not alter Th1 or Th17 cytokine (IFN-γ and IL-17) levels. Furthermore, 4-CMTB blocked DNCB-induced lymph node enlargement. In conclusion, activation of FFA2 ameliorated DNCB-induced atopic dermatitis, which suggested that FFA2 is a therapeutic target for atopic dermatitis.

• Natural Product Sciences
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• 제25권2호
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• pp.165-171
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• 2019

Although the functions of a standardized extract of Gingko biloba leaves (EGb $761^{(R)}$) has been reported with regard to neurobiological properties, no attention has been paid to the impact of EGb $761^{(R)}$ on the neuronal regulation of energy homeostasis. To evaluate the hypothesis that EGb $761^{(R)}$ affect the secretion of peptide tyrosine tyrosine (PYY) and the activation of free fatty acid receptor 4 (FFA4), which are involved in the neuronal circuitries that control energy homeostasis by inducing the transfer of information about the influx of energy to the brain, we examined whether EGb $761^{(R)}$ can stimulate PYY secretion in the enteroendocrine NCI-H716 cells and if EGb $761^{(R)}$ can activate FFA4 in FFA4-expressing cells. In NCI-H716 cells, EGb $761^{(R)}$ stimulated PYY secretion and the EGb $761^{(R)}$-induced PYY secretion was involved in the increase in intracellular $Ca^{2+}$ concentration and the activation of FFA4. Furthermore, in FFA4-expressing cells, EGb $761^{(R)}$ activated FFA4. These results suggest that EGb $761^{(R)}$ may affect the control of energy homeostasis via the regulation of PYY secretion and FFA4 activation.

• Journal of Ginseng Research
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• 제38권2호
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• pp.97-105
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• 2014

Background: Understanding what causes changes in the flux of free fatty acids (FFA) is important to elucidate the etiology of metabolic syndrome. The first aim of this study was to test whether or not hormones and the autonomic nervous system influence blood FFA levels. A secondary aim was to test by means of a multiple group path analysis whether the consumption of fermented red ginseng (FRG; Panax ginseng) would influence those causal relationships. Methods: Ninety-three postmenopausal women (age 50e73 yr) were randomly divided into two groups. One group (44 women; age, $58.4{\pm}5.9yr$; body mass index, $3.6{\pm}2.5kg/m^2$) was supplied place capsules and the other group (49 women, age $58.4{\pm}5.5yr$; body mass index, $22.9{\pm}2.4kg/m^2$) was supplied FRG capsules. Both prior to and after the study (2 wk), blood samples were collected from the participants and several blood variables were measured and analyzed. Results: Squared multiple correlations of FFA were 0.699 in the placebo group and 0.707 in the FRG group. The unstandardized estimate of estradiol (E2) for FFA was 0.824 in both groups. Conclusion: The path coefficients of cortisol and the branchial pulse for FFA were significantly different between the FRG group and the placebo group.

• 한국식품과학회지
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• 제22권5호
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• pp.526-529
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• 1990

국내에서 생산되고 있는 버터의 유리지방산 조성을 분석하기 위하여 silicic acid column chromatography 방법을 이용하여 버터로부터 직접 유리지방산을 분리 용출시켜 농축한 후 GLC로 분석하였다. 국산 버터 2종류 제품을 분석한 결과, 버터 A의 유리지방산 조성에 있어서는 $C_{18}$ 유리지방산$(C_{18_0},\;C_{18:1},\;C_{18:2}\;및\;C_{18:3})$ 함량이 52.83%로 가장 높았고, 그 다음으로 palmitic acid가 29.39%, myristic acid가 11.85% 함유되어 있었으며, 버터 B의 경우에는 $C_{18}$ 유리지방산이 47.50%, palmitic acid가 30.52%, myristic acid가 13.76% 함유되어 있었고, 그 외의 유리지방산은 소량씩 함유되어 있었다. 한편 함량면에서는 적지만 버터의 풍미에 중요한 영향을 미치는 것으로 알려진 butyric, caproic, capric 및 lauric acid의 농도를 분석한 결과, 버터 A에서는 각각 35, 10, 48, 60ppm, 버터 B에서는 각각 56, 11, 70, 126ppm이었다.

• 대한약리학회지
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• 제1권1호
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• pp.37-46
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• 1965

1. In the tortoise, Amyda japonica, a cold-blooded animal readily available in this country, the role of catecholamines in the regulation of free fatty acids(FFA) metabolism was investigated in both in vivo and in vitro studies. 2. Norepinephrine elevated both FFA and glucose levels in plasma. 3. When $50{\mu}g/kg$ of Epinephrine, Norepinephrine and Isopropylarterenol were administered intravenously, the relative effectiveness of mobilizing FFA was in the descending order of potency-Epinephrine, Norepinephrine and Isopropylarterenol. 4. In order to exclude the 'tonic influence of the endogenous catecholamines', reserpine was given to some animals. Two days after the reserpine-treatment, glucose showed a significant increase over the solely vehicle treated controls, FFA but an insignificant one. Excised auricles from those animals showed a diminished response to tyramine. Seven days after the treatment, however, when the depletion of catecholamines from the tissue stores seemed to be complete, judged from the absence of the response of isolated auricles to tyramine, both FFA and glucose levels were definitely lowered. 5. In in vitro experiments Epinephrine enhanced the FFA-release from the adipose tissue. The effect increased proportionately with the concentration until a maximal effect was attained at a concentration of 1x $10^5$ g/ml. 6. The order of potency in releasing FFA from adipose tissue in vitro was the same as in vitro, i.e., Epinephrine, Norepinephrine and Isopropylarterenol, but the differences were much less marked. 7. Ergotamine exerted no lipolytic action, but inhibited the lipolytic effect ef Epinephrine significantly. 8. Nethalide showed a slight lipolytic effect per se but inhibited the Epinephrine-induced lipolysis significantly. 9. Catecholamines play an important role in regulating FFA metabolism in the cold-blooded animal, just as in the warm-blooded animals, and the tortoise, Amyda japonica, may be used in the studies of fat metabolism as well as the rat.

• 한국세라믹학회지
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• 제44권9호
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• pp.502-509
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• 2007

This paper investigated how Fine Fly Ash (FFA) with $14,000\;cm^2/g$ of Fineness affects the micro structure and material properties of High-Performance Concrete (HPC) before and after hardening from Material Test of HPC and Cement Paste. FFA is applied as a substitute of Silica Fume which is used necessarily in producing HPC. As a Material Test results, 5% FFA series specimen shows the lower fluidity than SF series specimen. When, however, the Fluidity of 10% FFA series specimen is increased reversely to the similar value of SF series specimen. The Porosity of FFA series specimen of 3 day age is displayed to $21{\sim}24%$, which is higher than $19{\sim}20%$ porosity of SF series specimen, while that of 28 day age is reached to $8{\sim}9%$, which is improved compared with 10% fo SF series specimen. It can be thought that FFA has better influence on the porosity of HPC in case of long term age. The Compressive strength of FFA series specimen shows the similar result with the property of porosity. The compressive strength of 28 day age FFA series specimen is $98{\sim}106%$ of SF series specimen and 107% of plain specimen to reveal better strength development.

• 약학회지
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• 제25권2호
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• pp.49-55
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• 1981

To elucidate the effect of ginseng saponin on adrenaline-induced hyperglycemia and hyperlipidemia, ginseng saponin was administered before and after administration of adrenaline, and concentration of glucose, triglyceride(TG), and free fatty acid (FFA) as well as lipase activity in plasma was determined. 1) Glucose concentration was slightly increased by administration of ginseng saponin at 1 hour before and 10 minutes after adrenaline administration, but decreased by administration of ginseng saponin at 7 and 4 hours before adrenaline administration. TG and FFA concentration was also greatly inhibited by administration of ginseng saponin in advance. 2) When gineseng saponin 3, 10, 30mg/kg were administered 7 hours before adrenaline administration, glucose and TG concentration as well as lipase activity were inhibited in proportion to the dose of ginseng saponin, but FFA concentration was slightly inhibited. 3) It was suggested that protopanaxatriol group have potentiating effect on adrenaline induced hyperglycemia but protopanaxadiol group have inhibitory effect. And ginseng saponin seems to have strong inhibitory action on TG mobilization into blood and stimulatory effect on esterification of FFA in liver and adipose tissue.

• Journal of Animal Science and Technology
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• 제50권1호
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• pp.111-120
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• 2008

산양유의 비유기간 중 연평균 유지방 함량이 3.88% 이었으며, 동절기(4.2%) 보다 하절기 (3.8%)에 낮았다. 유지방 함유율이 높을수록 총고형분 함량도 비례하여 증가하였다. 산양유 원유를 24시간 냉장저장 하는 동안에 저급 유리지방산(C4:0~C10:0)은 106% 증가하였으며 중급 및 고급 유리지방산(C12:0~C18:1)은 203%로 높은 증가율을 보였다. 균질한 시유에 원유를 첨가하여 반응할 때에 저급 유리지방산은 22% 증가되었으며 중급 및 고급 유리지방산은 199% 증가되었다. Lipase의 종류에 따른 지방분해실험에서 calf lipase는 중급 및 고급지방산 9배 보다 저급지방산은 5.6배로 증가시켰다. 그러나 Candida rugosa lipase와 Pseudomonas fluorescens lipase는 저급 유리지방산이 각각 6배와 14배 증가하고, 중급 및 고급 유리지방산은 각각 34배와 162배 증가하여 중급 및 고급지방산을 더 잘 분해하는 것으로 나타났다. 산양유의 LPL 활성이 높을수록 유리지방산 생성량도 증가되어 유의적 상관관계(r=0.5635)를 보였으며 또한 산양유 이취가 높아지는 상관관계(r=0.5777)를 보였다. 유지방 함량이 낮을수록 LPL 활성은 높아지는 유의적 역 상관관계(r=－0.4627)를 보였다. 산양유의 palmitic acid 함량이 높을 경우 산양유 이취 강도가 증가하는 유의적 상관관계(r=0.7226)를 보였다.

• 산업식품공학
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• 제15권4호
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• pp.420-425
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• 2011

유리지방산(FFA), 글리세롤, MAG, 세사몰, 수용성 참깨박 추출물(ASM) 첨가에 의한 213$^{\circ}C$에서 21분 볶아 제조된 참기름의 180$^{\circ}C$ 열산화 시 세사몰과 세사몰린 함량 변화를 분석하였다. 90분간의 열산화에 의해 FFA와 MAG가 10% 첨가된 시료는 각각 0.94, 0.70 mM의 세사몰이 유의적으로 추가 생성되었으나 대조구는 0.09 mM만 증가하였다(p < 0.05). 세사몰린의 경우 FFA와 MAG 10% 첨가 시료는 대조구에 비해 15 및 18% 유의적으로 감소하였다 (p < 0.05). 1.5와 2.5 mM 첨가 세사몰은 세사몰린의 유의적인 변화를 유발하지 않았다(p > 0.05). ASM 첨가 시료에서 세사몰과 세사몰린의 일관적인 변화는 확인되지 않았다. 고온에서 볶은 참깨로부터 착유된 참기름의 가열 산화 시세사몰 생성 및 세사몰린 분해에는 FFA 및 MAG 같은 유지산화생성물의 함량에 주로 영향을 받는 것으로 사료된다.