• 한국수정란이식학회지
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• 제28권1호
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• pp.57-61
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• 2013

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.

• 한국기계가공학회지
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• 제12권6호
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• pp.69-76
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• 2013

This paper presents the development of the FCF method for the manufacturing of final products using numbers related to the minimum amount of work. The utilized product is a drum clutch, which is part of the transmission of an automobile. A double acting press is secured first and a prediction of the forming load on the practical material is made through an experiment with a plasticine model. Also, a finite element simulation using product shape and properties is performed, as well as a press experiment. A double acting press is manufactured that is suitable for a double acting experiment with a conventional hydraulic press(200 tons). A peripheral device for the press is additionally designed for experimental purposes. And, the press has as its essential points the drive speed, stroke control, etc., all of which influence the forming and is modified. Especially, a laser system is used for velocity measurement of two punches. The forming load of a practical material is predicted in order to derive a forming load formula for cold conditions on the basis of approximate similarity theory. Finite element analysis of the relative velocity ratio(RVR), etc., for most suitable flow defect(unfilling, etc.) prevention is achieved as well. The results are verified through a press experiment.

• KSBB Journal
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• 제25권5호
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• pp.459-465
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• 2010

A simple and efficient analytical method for the simultaneous determination of seven tar color additives was developed using ion pair high performance liquid chromatography. The conditions for HPLC analysis were as follows: column, ${\mu}$-Bondapak C18 (10 ${\mu}m$, 300 ${\times}$ 3.9 mm i.d.); gradient mobile phase, 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrile-methanol (65:25:10) as a mobile for fraction A and 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrilemethanol (40:50:10) as a mobile for fraction B; flow rate, 1.0 mL/ min; detection wavelength, 254/520/620 nm. We could attain to the detection limits as 0.01~0.05 ${\mu}$g/mL (254 nm) and 0.005~0.01 ${\mu}$g/mL (520 nm) for six red tar color additives, and 0.05 ${\mu}$g/mL (254 nm) and 0.002 ${\mu}$g/mL (620 nm) for Fast green FCF. This analytical method was applicable to determine the tar color additives contained in several commercial cold syrups.

• 정보처리학회논문지A
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• 제12A권2호
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• pp.117-126
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• 2005

대규모 멀티컴퓨터 시스템에서 단편화를 줄일 수 있는 적응성 있는 프로세서 할당 기법을 제안한다. 큰 크기의 서브메쉬 할당을 요구하는 작업들의 개수가 적을 경우에도 나머지 작업들의 대기 큐의 지연시간이 증가할 수 있다. 이런 상황에서 할당이 불가능한 기존의 기법들과 달리, L-모양 서브메쉬를 할당할 수 있으며, 할당 가능한 L-모양 서브메쉬를 효율적으로 탐색하는 알고리즘을 개발하였다. 그러므로 FCFS로 스케쥴링되어도, 대기 큐의 지연시간을 줄임으로써 평균응답시간을 줄일 수 있다. 시뮬레이션 결과를 통해서 제안하는 기법이 외부 단편화, 작업응답시간, 그리고 시스템의 활용도 면에서 다른 기법들보다 우수함을 보인다.

• 전기전자학회논문지
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• 제25권3호
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• pp.451-461
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• 2021

다양한 스마트 기기의 보급으로 인하여 악성코드로 인한 피해를 더욱 심각해지면서 머신러닝 기술을 활용한 악성코드 탐지 기술이 주목 받고 있다. 그러나 코드의 단편적인 특성만을 기반으로 머시러닝의 학습 데이터를 구성할 경우, 이를 회피하는 변종 및 신종 악성코드는 여전히 제작하기 쉽다. 이와 같은 문제를 해결하기 위한 방법으로 악성코드의 함수호출 관계를 학습 데이터로 사용하는 연구가 주목받고 있다. 특히, GNN을 활용하여 그래프의 유사도를 측정함으로써 보다 향상된 악성코드 탐지가 가능할 것으로 예상된다. 본 논문에서는 GNN을 악성코드 탐지에 활용하기 위해 바이너리 코드로부터 함수 호출 그래프를 생성하는 효율적인 방안을 제안한다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1549-1556
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• 2012

Endophytic fungi were isolated from roots of six halophytes in Suncheon Bay. The endophytic fungi of 35 species isolated from halophytes were identified by internal transcribed spacer (ITS) containing the ITS1, 5.8s, and ITS2 regions. All fungal strains were analyzed to diversity at the genus level. Fungal culture filtrates (FCF) of endophytic fungi were treated to Waito-c rice (WR) seedling for plant growth-promoting verification. It was confirmed that fungal strain Sj-2-2 provided plant growth promotion (PGP) to WR seedling. Then, PGP of Suaeda japonica was confirmed by treating culture filtrate of Sj-2-2. As a result, it was verified that culture filtrate of Sj-2-2 had more advanced PGP than positive control when treated to S. japonica. The secondary metabolites involved in culture filtrate of Sj-2-2 were identified by HPLC and GC-MS SIM analysis. The presence of physiologically bioactive gibberellins (GAs) and other inactive GAs in culture filtrate of Sj-2-2 was detected. The molecular analysis of sequences of Sj-2-2 showed the similarity to Penicillium sp. of 99% homology. The PGP of Sj-2-2 as well as symbiosis between endophytic fungi and halophytes growing naturally in salt marsh was confirmed. Sj-2-2 was identified as a new fungal strain producing GAs by molecular analysis of sequences. Consequently, the Sj-2-2 fungal strain was named as Penicillium sp. Sj-2-2. In this study, the diversity of endophytic fungi isolated from roots of halophytes in salt marsh and the PGP of a new gibberellin-producing fungal strain were confirmed.

• 산업경영시스템학회지
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• 제41권2호
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• pp.153-158
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• 2018

At the Bank of Korea, capital stock statistics were created by the PIM (perpetual inventory method) with fixed capital formation data. Asset classifications also included 2 categories in residential buildings, 4 non-residential buildings, 14 constructions, 9 transportation equipment, 28 machinery, and 2 intangible fixed assets. It is the Korean government accounting system which is developed much with the field of the national accounts including the valuation, but until 2008 it was consistent with single-entry bookkeeping. Many countries, including Korea, were single-entry bookkeeping, not double-entry bookkeeping which can be aggregated by government accounting standard account. There was no distinction in journaling between revenue and capital expenditure when it was consistent with single-entry bookkeeping. For example, we would like to appropriately divide the past budget accounts and the settlement accounts data that have been spent on dredging into capital expenditure and revenue expenditure. It, then, tries to add the capital expenditure calculated to FCF (fixed capital formation), because revenue expenditure is cost for maintenance etc. This could be a new direction, especially, in the estimation of capital stock by the perpetual inventory method for infrastructure (SOC, social overhead capital). It should also be noted that there are differences not only between capital and income expenditure but also by other factors. How long will this difference be covered by the difference between the 'new series' and 'old series' methodologies? In addition, there is no large difference between two series by the major asset classification level. If this is treated as a round-off error, this is a problem.

• Journal of Construction Engineering and Project Management
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• 제1권3호
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• pp.1-8
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• 2011

Available data on the recent global financial crisis (GFC) show that it lasted between the second quarter (Q2) of 2007 and the fourth quarter (Q4) of 2009. Australia is one of the first economies to fully recover from this crisis. This study explorles the role played by the Australian construction industry in stimulating economic growth during the recession. In order to investigate the macro-variability trend during the financial crisis, data were collected and analysed relating to the quarterly GDP of Australia and selected countries between Q1 2000 and Q4 2009. Specifically, changes in the construction industry's GDP were compared with aggregate GDP changes in Australian economy and similar indices in the 'Group of 7' (G7) countries and Organisation for Economic Co-operation and Development (OECD) countries. Moreover, specific attention was focused on Germany, France, Japan, United States of America (USA) and United Kingdom (UK). Graphical and Pearson's correlation methods were used to analyse the relationships between changes in construction GDP and Australia's overall economic growth during the recession. In addition, an attempt was made to develop a regression model for predicting economic growth during the recent recession using changes in gross fixed capital formation (GFCF), changes in construction GDP and the impact of these changes on national economy. Analysis shows a slight contraction in construction activities during the crisis; however construction triggered significant growth in the economy during the crisis period and afterwards. This appears to be the major difference between Australia and other major economies that have experienced a longer recession.

• 전기학회논문지
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• 제62권1호
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• pp.109-119
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• 2013

This paper presents the design of the Safety Programmable Logic Controller (SPLC) used in the Nuclear Power Plants, an analysis of a reliability for the SPLC using a markov model. The architecture of the SPLC is designed to have the multiple modular redundancy composed of the Dual Modular Redundancy(DMR) and the Triple Modular Redundancy(TMR). The operating system of the SPLC is designed to have the non-preemptive state based scheduler and the supervisory task managing the sequential scheduling, timing of tasks, diagnostic and security. The data communication of the SPLC is designed to have the deterministic state based protocol, and is designed to satisfy the effective transmission capacity of 20Mbps. Using Markov model, the reliability of SPLC is analyzed, and assessed. To have the reasonable reliability such as the mean time to failure (MTTF) more than 10,000 hours, the failure rate of each SPLC module should be less than $2{\times}10^{-5}$/hour. When the fault coverage factor (FCF) is increased by 0.1, the MTTF is improved by about 4 months, thus to enhance the MTTF effectively, it is needed that the diagnostic ability of each SPLC module should be strengthened. Also as the result of comparison the SPLC and the existing safety grade PLCs, the reliability and MTTF of SPLC is up to 1.6-times and up to 22,000 hours better than the existing PLCs.

• 대한본초학회지
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• 제33권3호
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• pp.11-18
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• 2018

Objectives : Saururus chinensis and Houttuynia cordata (Saururaceae) are perennial herbs using for medicinal purposes in Korea. The objectives of this study are to compare anatomical key characters between two medicinal plants and to provide fundamental information for the identification of two herbal medicines by using anatomical features. Methods : Cross-sections of root, rhizome, stem, petiole, and leaf for each species were observed in this study. Materials were analyzed through dehydration, paraffin embedding and micro-sectioning, and double staining with Safranin O and Fast-Green FCF. Observations of permanent preparation were conducted using light microscope. Results : S. chinensis and H. cordata were distinguished with anatomical differentiations; Idioblasts with essential oil were scattered in the parenchyma cell of cortex, pith, and phloem of S. chinensis, on the other hand, in H. cordata, idioblasts were distributed ring-shaped in the cortex of the root. S. chinensis had two cycles of vascular bundles in the stem while H. cordata had one cycle. Hypodermis layer was conspicuous in a stem of H. cordata, crystals were observed the only parenchyma in a stem of S. chinensis, and epidermal oil cells were developed in the epidermis of H. cordata. S. chinensis had air cavity at the cortex and pith of the stem. The shape of cross-section was polygonal in the stem of S. chinensis and was circular in the stem of H. cordata. Conclusions : We investigated anatomical study of Korean S. chinensis and H. cordata. To identify two herbal medicines, we considered main anatomical features and provided identification key here.