• Korean Journal of Microbiology
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• v.38 no.3
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• pp.162-167
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• 2002

To analyse proteins and gene related to antifungal activity, SAR01 strain was isolated from a brown seaweed and identified as Streptomyces sp. by FAME(fatty acid methyl ester) analysis. Antifungal activity deficient mutant(SAR535) of Streptomyces sp. SAR01 was induced by gamma radiation$({60}^Co)$. It was found that 6 specific protein spots appeared only in SAR01 by 2-D electrophoresis analysis. Among them, a protein of 10 kDa had homology of 96% with 10 kD chaperonin cpn 10 (GroES) by Basic Local Alignment Search Tool(BLAST, NCBI) analysis. SAR535 transformants into which groES was transferred by electroporation revealed antifungal activity newly similar with SAR01 It suggested that groES be supposed to be related to the antifungal activity of Streptomyces sp. SAR01.

• Journal of Industrial Convergence
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• v.2 no.1
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• pp.159-186
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• 2004

One of the biggest change in the distributive market recently is that the development of private brand is progress actively since consumers purchasing behavior is tend to shift to resonable and practical planned purchase from brand image or brand name. Once the development of private brands is done successfully, the independent merchandising distinguished from other distribution companies is established, store image through customer satisfaction is strengthen and volume of sales and profit are increased. However, the existing studies in this field have not dealt with this matter systematically. Therefore, the purpose of this study is to propose an integrated framework within which to view private brand proneness. Factors found to influence private brand proneness included perceived cost, with private brand, perceived value for money, store fame, perceived risk, perceived quality variation between national and private brand products. Foe empirical analysis, the data were collected from 211 consumers who purchased the private brands in discount store and department store. With the collected survey data, reliability test, factor analysis for validity test and regression analysis for hypotheses test were conducted. Most hypotheses were accepted but on hypotheses were rejected negative relation of perceived cost and private brand proneness. Research results indicate that private brand proneness is affected by perceived value, perceived fame and there are negative relation between private brand proneness and perceived quality variation.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.2
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• pp.144-156
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• 2016

Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.42 no.3
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• pp.95-108
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• 2019

Quality management has become an pervasive philosophy in most sectors of business. Specific movements such as statistical quality control, quality circle, total quality management, and quality management system have become embedded in business organizations. Only the companies with competitive edge can survive in the competition in global market. KSA(Korean Standards Association) established in 1962 has launched all kinds of quality education, quality standard certification service for business, and KNQA(Korean National Quality Award) system. This article considers quality competitiveness excellent company award among KNQA. We performed a statistical analysis of audit data for quality competitiveness excellent company for three years, from 2015 to 2017. By using ANOVA and two sample t-tests, the average scores of 13 evaluation fields were significantly different depending on company size and type. We proposed ways to improve the current hall of fame system. We discovered that the average scores of 13 evaluation fields in the audit data according to years and hall of fame status were not significantly different. We also showed linear relationships among 13 evaluation fields by correlation analysis and obtained an estimated linear regression equation : Business Performance, which is a comprehensive index, as a dependent variable was significantly related to Customer Focus and Product Liability as regressor variables among 13 evaluation fields by regression analysis.

• Applied Chemistry for Engineering
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• v.18 no.3
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• pp.284-290
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• 2007

Biodiesel and its blend fuels from soybean oil were characterized for their oxidation to apply automobile fuel from the analysis of FAME (fatty acid methyl ester) and chemical properties. Biodiesel produced from soybean oil contained unsaturated fatty acids (> 85 wt%) such as oleic acid, linoleic acid, and linolenic acid. Especially, polyunsaturated fatty acids such as linoleic acid and linolenic acid containing active methyl radical were over 60 wt%. It is believed that linoleic acid and linolenic acid cause oxidation. Linoleic acid and linolenic acid during oxidation were major reactants, and compounds with the carbon number having around 36 (boiling point of about $500^{\circ}C$) were produced from those of radical autoxidation.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.12 no.1
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• pp.11-16
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• 2016

Fuel assembly's mechanical characterization test facility (FAMeCT) in KAERI was constructed with upgraded functional features such as increased loading capacity, underwater vibration testing and severe earthquake simulation for extended fuel design guideline. This facility is designed and developed to provide out-pile fuel data for accident analysis model and fuel licensing. Functional tests of FAMeCT were performed to confirm functionality, structural integrity, and validity of newly-built fuel assembly mechanical test facility. Test program includes signal check of data acquisition system, load delivering capacity using real-sized fuel assemblies and a standard loading cylindrical rigid specimen. Fuel assembly's lateral bending test was carried out up to 30 mm of pull-out displacement. Limit case axial compression loading test up to 33 kN was performed to check structural integrity of UCPS (Upper Core Plate Simulator) support frame. Test results show that all test equipment and measurement system have acceptable range of alignment, signal to noise ratio, load carrying capacity limit without loss of integrity. This paper introduces newly constructed fuel assembly's mechanical test facility and summarizes results of functional test for the mechanical test equipment and data acquisition system.

• Korean Chemical Engineering Research
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• v.56 no.5
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• pp.773-778
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• 2018

In-situ transesterification of microalgae lipids using microwave irradiation has potential to simplify and accelerate biodiesel production, as it minimizes production cost and reaction time by direct transesterification of microalgae into biodiesel with microwave as a heating source. This study was conducted to research the effect of microwave irradiation with in-situ transesterification of microalgae under base catalyst condition. The process variables (reaction time, solvent ratio, microwave power) were studied using 2% of catalyst concentration. The maximum yield of FAME was obtained at about 32.18% at the reaction time of 30 min with biomass-methanol ratio 1:12 (w/v) and microwave power of 450 W. The GC MS analysis obtained that the main component of FAME from microalgal oils (or lipids) was palmitic acid, stearic acid and oleic acid. The results show that microwaves can be used as a heating source to synthesize biodiesel from microalgae in terms of major components resulting.

• Korean Journal of Environmental Biology
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• v.20 no.3
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• pp.237-244
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• 2002

To analyze proteins related to antifungal activity, SAR01 strain was isolated from seaweed and identified as Streptomyces sp. from the result of FAME (fatty acid methyl ester) analysis. The isolated strain had antifungal activities against T species of plant pathogenic fungi. Antifungal activity deficient mutant (SAR 535) of Streptomyces sp. SAR01 was induced by gamma radiation $(^{60}Co,\;5kGy)$. By 2 D electrophoresis analysis, 6 protein spots were found in wild strain (SAR01) but these spots disappeared in mutant strain (SAR535). Among them, 5 proteins showed similarities to heat shock protein 70(HSP70), Fe-containing superoxide dismutase II (Fe- SODII), ribosome recycling factor (RRF), 10 kDa chnperonin (GroES) and inorganic pyrophosphatase (PPAse), respectively. It suggested that the above 6 proteins could be closely related to the antifungal activity of Streptomyces sp. SAR01.

• Analytical Science and Technology
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• v.30 no.4
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• pp.174-181
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• 2017

Bio-liquid is a liquid by-product of the hydrothermal carbonization (HTC) reaction, converting wet biomass into solid hydrochar, bio-liquid, and bio-gas. Since bio-liquid contains various compounds, it requires efficient sampling method to extract the target compounds from bio-liquid. In this research, fatty acid methyl ester (FAME) in bio-liquid was extracted based on hollow fiber supported liquid phase microextraction (HF-LPME) and determined by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography/Mass Spectrometry (GC/MS). The well-known major components of biodiesel, including methyl myristate, palmitate, methyl palmitoleate, methyl stearate, methyl oleate, and methyl linoleate had been selected as standard materials for FAME analysis using HF-LPME. Physicochemical properties of bio-liquid was measured that the acidity was 3.30 (${\pm}0.01$) and the moisture content was 100.84 (${\pm}3.02$)%. The optimization of HF-LPME method had been investigated by varying the experimental parameters such as extraction solvent, extraction time, stirring speed, and the length of HF at the fixed concentration of NaCl salt. As a result, optimal conditions of HF-LPME for FAMEs were; n-octanol for extraction solvent, 30 min for extraction time, 1200 rpm for stirring speed, 20 mm for the HF length, and 0.5 w/v% for the concentration of NaCl. Validation of HF-LPME was performed with limit of detection (LOD), limit of quantitation (LOQ), dynamic range, reproducibility, and recovery. The results obtained from this study indicated that HF-LPME was suitable for the preconcentration method and the quantitative analysis to characterize FAMEs in bio-liquid generated from food waste via HTC reaction.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.276-280
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• 2002

To find the applicability of resemblance of the fatty acid methy1 ester (FAME) profile analysis to the primary examination for the tracing P. aeruginosa contamination in the environment and water plants, we designed this study. Of6l P. aeruginosa isolates including 3 reference strains, 4 fecal or soil isolates and 54 water isolates, the resemblance coefficiencies of the FAMEs were calculated as euclidian distance (ED) and expressed as dendrogram. The 61 strains were clustered as the same group in the ED 8.4, and divided into 4 subgroups in the ED 6.9. The water isolates from Han River were classified into distinctively different subgroups from reference strains. These results provide the possibility of the dendrogram from FAME profile as a useful tools for tracing contamination as well as for identifying the species, especially in case of P. aeruginosa.