• Parasites, Hosts and Diseases
• /
• 제25권2호
• /
• pp.141-148
• /
• 1987

실험적으로 폐홉춘을 감염시킨 백서의 혈청내 항원을 ELISA 법으로 측정하였다. 시험용 혈청은 폐흡충의 피낭유충을 마리당 25개씩 먹인 총 22마리의 백서에서 감염후 12주가 될 때까지 채혈하여 얻었다. 폐흡충에 대한 특이항체는 폐흡충 감염 고양이의 혈청으로부터 ammonium sulfate 침전과 anionezchange chromatography를 이용, IgG를 분리한 후 affinity chromatography를 이용하여 순수분리하였다. 항원을 찾기 위한 ELISA는 소위 "double antibody sandwich method"를 사용하였다. 대조로서 사용한 감염전 혈청의 O.D.치 평균값은 0.04(표준편차 0.04)이었으며 감염후 O.D.치 (표준편차)의 변화를 보면 0.5주(3일)는 0.03(0.01), 1주는 0.55(0.50), 1.5주는 0.69(0.45), 2주는 0.20(0.19), 2.5주는 0.13(0.10)이었고 감염 3주 이후는 대조 혈청의 값으로 떨어졌다. 대조혈청의 $평균간+3{\times}$ 표준편차, 즉 0.16이하를 이 system에서의 비특이적 수치로 잡았을 때 이 이상의 수치를 나타낸 경우는 감염 후 1주부터 2.5주까지의 혈청에서만 관찰되었다. 한편 감염 12주 후에 총 10마리의 백서를 희생시켜 유충을 회수하여 보았다. 폐와 흉강에서는 평균 2.2마리, 근육에서는 평균 6.2마리의 유충을 회수할 수 있었다. 폐흡충 감염 백서에 있어 충체가 생산하는 항원은 감염 1주 후부터 혈청 내에서 검출되었다. 그러나 감염 3주후부터는 검출되지 않았는데 이것은 항원-항체 복합체 형성에 의한 반응 저해 현상에 의한 것으로 생각되었다.

• Molecules and Cells
• /
• 제38권1호
• /
• pp.1-13
• /
• 2015

Recent technical developments have transformed how neuroscientists can probe brain function. What was once thought to be difficult and perhaps impossible, stimulating a single set of long range inputs among many, is now relatively straight-forward using optogenetic approaches. This has provided an avalanche of data demonstrating causal roles for circuits in a variety of behaviors. However, despite the critical role that neuropeptide signaling plays in the regulation of behavior and physiology of the brain, there have been remarkably few studies demonstrating how peptide release is causally linked to behaviors. This is likely due to both the different time scale by which peptides act on and the modulatory nature of their actions. For example, while glutamate release can effectively transmit information between synapses in milliseconds, peptide release is potentially slower [See the excellent review by Van Den Pol on the time scales and mechanisms of release (van den Pol, 2012)] and it can only tune the existing signals via modulation. And while there have been some studies exploring mechanisms of release, it is still not as clearly known what is required for efficient peptide release. Furthermore, this analysis could be complicated by the fact that there are multiple peptides released, some of which may act in contrast. Despite these limitations, there are a number of groups making progress in this area. The goal of this review is to explore the role of peptide signaling in one specific structure, the bed nucleus of the stria terminalis, that has proven to be a fertile ground for peptide action.

• 대한핵의학회지
• /
• 제5권2호
• /
• pp.19-40
• /
• 1971

Since the iron balance is maintained by regulated intestinal absorption rather than regulated excretion, there have been many reports concerning the factors which may influence the intestinal iron absorption. As the liver is the largest iron storage organ of the body, any hepatocellular damage may result in disturbances in iron metabolism, e,g., frequent co-existence of hemochromatosis and liver cirrhosis, or elevated serum iron level and increased iron absorption rate in patients with infectious hepatitis or cirrhosis. In one effort to demonstrate the influence of hepatocellular damage on intestinal iron absortion, the iron absorption rate was measured in the rabbits whose livers were injured by a single subcutaneous injection of carbon tetrachloride (doses ranging from 0.15 to 0.5cc per kg of body weight) or by a single irradiation of 2,000 to 16,000 rads with $^{60}Co$ on the liver locally. A single oral dose of $1{\mu}Ci\;of\;^{59}Fe$-citrate with 0.5mg of ferrous citrate was fed in the fasting state, 24 hours after hepatic damage had been induced, without any reducing or chelating agents, and stool was collected for one week thereafter. Serum iron levels, together with conventional liver function tests, were measured at 24, 48, 72, 120 and 168 hours after liver damage had been induced. All animals were sacrificed upon the completing of the one week's test period and tissue specimens were prepared for H-E and Gomori's iron stain. Following are the results. 1. Normal iron absorption rate of the rabbit was $41.72{\pm}3.61%$ when 0.5mg of iron was given in the fasting state, as measured by subtracting the amount recovered in stool collected for 7 days from the amount given. The test period of 7 days is adequate, for only 1% of the iron given was excreted thereafter. 2. The intestinal iron absorption rate and serum iron level were significantly increased when the animal was poisoned by a single subcutaneous injection of 0.15cc. per kg. of body weight of carbon tetrachloride or more, or the liver was irradiated with a single dose of 12,000 rads or more. The results of liver function tests which were done simultaneously remained within normal limit except SGOT and SGPT which were somewhat increased. 3. In each case, there has been good correlation between the extent of liver cell damage and degree of increased iron absorption rate or serum iron level. 4. The method of liver damage appeared to make no obvious difference in the pattern of iron deposit in liver. This may be partly due to the fact that tissue specimens were obtained too late, for by this time the elevated serum iron level had returned within normal range and the pathological changes were almost healed. 5. The possible factors and relationship between intestinal iron absorption and hepatic parenchymal cell damage has been discussed.

• 한국산림과학회지
• /
• 제25권1호
• /
• pp.49-71
• /
• 1975

The importance of bamboo as raw material for bamboo wares and several kinds of industrial products is highly appreciated at home and abroad. But different kinds of bamboo-bundle systems have been traditionally used in the local areas. There being no reasonable bamboo-bundle system, we have a lot of difficulty in trading bamboo products and executing adrinistmative works. Therefore, a reasonable bamboo-bundle system based on scientific proofs needs to be tested and established for fair trade and administration. This study is carried out to solve the above difficulty with statistical investigation and analysis. The results obtained are as follows. 1. The larger the circles at eye-height become, the more the possibility of the largest internode covering the span between eye-height and 1/4 height increases. 2. The longest internodes are distributed according to a rule without relation to circles at eye-height. 3. The tapering grade of bamboo culms is very high and its form is almost the same without relation to its size. (Form exponent; 0.71-1.05, eye-height form factor; 0.60-0.66, 1/4 becomes, seeing that the circle grade and the percentage of actual volume height form factor; 0.61-0.69). 4. The larger the circles at eye-height are, the lower the percentage of actual volume have negative curve relation to each other. 5. It is considered that the numbers of bamboos bundled in a "Sok" is not decided according to the usefulness of bamboos, judging from the fact that the outputs of bamboo wares per "Sok" in every circle grade are not the same. 6. As the results of the regression analysis, the empirical formulae of several amounts to circles at eye-height and culm length are as follows; Volume, $${\hat{y}}_i=\bar{3}.821874+2.013181log\;C_i+0.839128log\;H_i$$ $$V=0.0066355\;C^{2.013181}\;H^{0.839128}$$ Actual volume, $${\hat{y}}_{ai}=3.915338+0.776549log\;C_i+1.857000log\;H_i$$ $$V_a=0.0082288\;C^{0.776549}\;H^{1.857000}$$ Weight, $$w_i=3.869148+1.936410log\;C_i+0.566904log\;H_i$$ $$W=0.0073986\;G^{1.936410}\;H^{0.565904}$$ 7. Korean Phyllostachys bambusoides Sieb. et Zucc. is almost the same as that of Japan in several amounts, just the same especially in the weight. 8. It is found that the bamboo-bundle systems of Korea and Japan have much closer relation to the weight than other amounts. So It is, therefore, considered that the weight is important factor in deciding bamboo-bundle system. 9. According to the item 8, I should like to propose the appropriate numbers per "Sok" adjusted on the basis of the weight in the Table 18.

• Journal of Microbiology and Biotechnology
• /
• 제31권11호
• /
• pp.1583-1590
• /
• 2021

Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

• 정보보호학회논문지
• /
• 제32권3호
• /
• pp.513-525
• /
• 2022

블록체인의 불변성은 블록체인의 보안을 담당하는 주요 요인으로 데이터 무결성을 보장한다. 그러나 블록체인에 한 번 기록된 데이터에 대한 수정이 불가하다는 특성은 특정 사용자에 의해 악용될 여지가 존재한다. 실제로 비트코인 상에는 유해 데이터, 사용자 개인 데이터와 같이 블록체인에 기록되기에 부적절한 콘텐츠들이 노출되어 있다. 블록체인에 존재하는 부적절한 콘텐츠를 관리하는 방안으로는 G. Ateniese 등에 의해 처음으로 제안된 카멜레온 해시 기반 수정 가능한 블록체인이 존재한다. 수정 가능한 블록체인은 데이터 수정·삭제가 가능하다는 특성으로 GDPR의 잊힐 권리(right to be forgotten)를 충족하며, 최근 개인 데이터 관리에 관한 연구가 활발히 진행되고 있다. 그중 Y. Jia 등의 연구에서는 수정 가능한 블록체인 상에서 사용자 개인 데이터 관리가 가능한 모델을 제안하였으나 블록체인 참여 노드인 준 신뢰하는 규제자(semi-trusted regulator)가 블록체인에서 모든 블록에 대한 수정 권한과 트랜잭션 권한 박탈 등의 과도한 권한을 가지고 있어 부작용의 소지가 존재한다. 따라서 본 논문에서는 Y. Jia 등의 연구에서 규제자의 권한을 약화하고자 권한 주체 변경 및 권한 공유 방법을 제시하고 적용 가능한 시나리오를 토대로 수정 가능한 블록체인 기반 권한 변경 및 접근 제어 시스템 모델을 제안한다.

• 한국측량학회지
• /
• 제40권2호
• /
• pp.119-133
• /
• 2022

측량, 지도, 통신 기술의 발달로 다양한 길안내 앱 및 차량용 내비게이션 등이 개발 및 보급되었다. 그 결과, 보행 및 운전 전반에 널리 활용되며 많은 도움을 주고 있으나 여전히 사회적 약자의 일상 편의를 향상시키는데 있어서는 한계가 존재한다. 이는 일반 길안내 앱이나 내비게이션이 일반인의 보행이나 차량을 중심으로 서비스되고 있어 시각장애인이 이동 시 혼란을 유발하거나 위험 요소가 되는 정보를 제공하지 못하는데 따른 것이다. 이에 본 연구에서는 시각장애인과 같이 보행(이동)에 제약을 받는 교통약자를 대상으로 한 공간정보 기반 서비스를 구현하기 위한 데이터 구축 방안을 모색하고자 하였다. 이를 위해 공간정보 데이터를 기반으로 시각장애인의 보행과 관련되어 요구되는 항목과 구성요소를 선정하고, 이를 기반으로 상용 앱(app)에 접목할 수 있는 보행용 길안내 네트워크를 구성·분석하였다. 그 결과, 공간정보 기반의 점자블럭(점형/선형), 음향신호기, 버스정류장, 볼라드 등의 추가적인 시각장애인용 컨텐츠 정보가 확보될 경우 교통약자의 독립적인 보행을 위한 길안내 서비스가 가능하다는 결론을 도출했다. 또한, 길안내를 위한 데이터 모델을 정립하고 이를 기반으로 초기 버전의 모바일 앱을 구현했으며, 시각장애인을 대상으로 한 검증을 통해 시각장애인용 컨텐츠 정보 제공이 보행 시 유용함을 확인했다. 보다 안정적인 보행을 지원하기 위해서는 타 기관 또는 여러 부처에서 제공되는 공간정보 자료의 DB (DataBase)화 및 활용이 필요하며, 동시에 ICT (Information and Communications Technologies)기술 접목을 통해 다양한 센서 및 음성기술 등을 연계·활용한 고도화 방안도 모색되어야 할 것으로 판단된다.

• 한국해양바이오학회지
• /
• 제13권2호
• /
• pp.76-85
• /
• 2021

Crassostrea gigas were fermented using L. brevis BJ20 to prepare fermented oyster extract (FO). The participants of this study were randomly assigned to FO and placebo (CON) groups. The FO group was given 1.0 g of FO supplementation and the CON group was given sucrose each day for eight weeks. The effects of FO supplementation on body composition, muscula r strength, and blood factors associated with muscle growth were assessed. The FO supplement was enriched with arginine (6,183.3 mg), phenylalanine (217.9 mg), leucine (122.6 mg), isoleucine (59.8 mg), valine (16.4 mg), and γ-amino butyric acid (GABA, 1,053.7 mg). The total fat was significantly decreased in the FO group compared with the CON group (p < 0.05). 60D/S Ext.T/Wo rk and 60D/S Flex.T/Work concomitantly with 60D/S Flex.PeakTQ/BW were significantly increase d by FO treatment compared to CON group (p < 0.05). However, posture stability was not significa ntly different between the groups. The levels of angiotensin-converting enzyme were significantly decreased within the FO group (p < 0.05). The FO group showed significantly decreased levels of tumor necrosis factor-α and increased levels of human growth hormone compared with the CON group (p < 0.01). The levels of insulin-like growth factor-1 increased (p < 0.01) in the FO group while that of creatine kinase and triglyceride decreased significantly compared with the CON group (p < 0.05). These results demonstrated that FO supplementation is effective in preventing sarcopenic obesity and maintaining and strengthening muscular function in elderly wom en. Hence, FO supplements can be used as functional ingredients for these benefits.

• 산업융합연구
• /
• 제20권10호
• /
• pp.121-129
• /
• 2022

본 연구의 목적은 코로나19 상황에서 임상간호사의 간호업무수행에 영향을 주는 영향요인을 파악하여 간호업무수행 향상을 위한 기초자료를 제공하고자 시도되었다. 본 연구는 서술적 연구로서 G시에서 임상간호사로 근무하고 있는 119명을 대상으로 2022년 6월 15일부터 7월 20일까지 설문지를 이용하여 자료수집 하였다. 분석을 위해 서술적 통계, t-test, ANOVA, Pearson's correlation coefficients, Multiple regression으로 분석하였다. 연구결과, 감염관리수행(r=.553, p<.001), 회복탄력성(r=.286, p<.001)이 간호업무수행에 통계적으로 유의한 상관관계를 보였다. 임상간호사의 간호업무 수행에 영향을 미치는 요인으로 감염관리수행(B=.529, p<.001)이 18.3% 설명력을 나타냈다. 이와 같은 결과는 코로나19 유행상황에서 코로나19 감염관리 자체가 간호업무수행에 새로운 업무부담으로 작용했기 때문으로 생각된다. 반면 회복탄력성과 직무스트레스가 영향력을 미치지 못하였다. 그러나, 본 연구대상자의 직무스트레스가 매우 높아 임상간호사의 주기적인 상담을 통해 임상간호사의 직무스트레스를 낮추어 양질의 간호업무 수행이 가능하게 해야 할 것이다.

• 한국항만학회지
• /
• 제3권1호
• /
• pp.3-33
• /
• 1989

The amount of the Korean export & import in 1987 reached $88.3 billion which was 1.75% of the total world trade and the proportion of foreign dependence to G.N.P was 74.5%. From these facts, we can infer that the development of national economy is largely dependent upon trade. Therefore the role of transportation, especially Ocean transportation, as a basis of economic development through trade is one of the main factors that can not be passed over. Here, We can define that a port as a subsystem of transportation determines the efficiency of the total transportation system. Thus, the purpose of this paper is to contribute in improvement of the efficiency in port, reinforcement of the international competitiveness for exporting goods by the analysis of the cargo handling charges. In order to do this, this paper deals the case of B.C.T.O.C. Furthermore, this study gives some important informations related to the level of tariffs for establishing an autonomous port administration. The Summary of the conclusions of this paper is as follows ; 1) The object of port administration in Korea has been emphasized on the maximization of efficiency in using the port facilities. Nowadays, however, it should be moved to a direction that port is operated under the compound aims considering the public interests and economy. 2) For a criterian of tariff calculation, A tariff system based on the cost accounting is desirable. In general it is recommended that the cost for construction, management, and operation of port is compensated by the revenue from port operation. Therefore, it is necessary for the administration bodies of each port to establish a tariff system on the basis of the independent profit system. 3) For the investigation of actors of tariff adjustment by the Break-even point analysis, (1) When we conducted the B.E.P analysis using total cost as cost term, we got 3.8% discount in tariff at 12% of target profit rate and 1.5% discount at 15% of rate. when we set the target profit rate as 17% we could have the proper tariff level. (2) When using operating cost as cost term, we got 13.1% discount in tariff at 12% of target profit rate and 10.9% discount at 15% of rate. When setting the target profit rat as 28%, we could have the proper tariff level. 4) Comparing with the tariffs of foreign ports for the basic terminal rate, The tariff level of B.C.T.O.C showed 33% of stevedoring charge and 80% of marshalling charge incurred at Kobe port. The comparison with Singapore port gave 50% of transhipment charge and 17% - 20 % of stevedoring charge. 5) We found that the financial structure of B.C.T.O.C was better than those of other companies and the worth fixed assets ratio was too low. The fact of low worth fixed assets ratio implies that the cargo handling facilities should be increased. Moreover, The return of assets for B.T.T.O.C was good but non-operating expenses were still contained too much in. Therefore, we think that it is necessary for B.C.T.O.C. to rationalize business management. Although the present cargo handing charge for B.C.T.O.C is a proper level in terms of a public corporation, for the final recommendation in connection to the results, It is required to take the rationalization process for business management.