• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.13-16
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• 2008

Pepper fruit anthracnose caused by Colletotrichum species is an economically important disease that causes serious yield loss and quality deterioration in many Asian countries including South Korea and Taiwan. Recently, Capsicum baccatum PI594137 was found to exhibit broad-spectrum resistance to Colletotrichum acutatum. The inheritance of anthracnose resistance to C. acutatum was analyzed in an intraspecific population derived from a cross between C. baccatum Golden-aji and PI594137. Detached mature green fruits were inoculated using the microinjection method. The disease response was evaluated as the disease incidence and the overall lesion diameter at 7 days after inoculation(DAI). The segregation ratios of resistance and susceptibility to C. acutatum in the $F_2$ and $BC_s$ populations significantly fit a 3:1 Mendelian model. This result indicates that the resistance of PI594137 to C. acutatum is controlled by a single dominant gene.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8837-8842
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• 2014

Background: Hepatitis C virus (HCV) infection is an important cause of liver cancer in Thailand. The highest prevalence of anti-HCV positive among Thai blood donors is found in the northeastern region. The present analysis of the genotype distribution among anti-HCV positive northeastern-Thai blood donors was conducted to provide a base for the epidemiological pattern of HCV infection in this region. Materials and Methods: A total of 112 HCV seropositive healthy blood donors were randomly selected and tested for the presence of HCV-RNA by RT-PCR. HCV-RNA positive samples were genotyped by direct sequencing at core region genomes and confirmed by phylogenetic analysis. Results: HCV viremia was found in 94.6% (106/112) of HCV seropositive blood donors. There were 3 major genotypes distributed among this population. HCV genotype 3a was the most prevalent (71.7%) followed by genotypes 1a (7.5%), 1b (7.5%), 6i (3.8%), 6f (2.8%) and 6n (1.9%). Conclusions: HCV genotype 3a in asymptomatic infections in northeastern Thailand is significantly higher than other previous reports. Subgenotype 6 prevalence is less than in neighboring countries and distribution patterns differ. The findings are relevant as predictors for using interferon therapy in this population.

• Bulletin of the Korean Space Science Society
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• 2010.04a
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• pp.34.4-34.4
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• 2010

We present the properties of globular cluster systems for 10 early-type galaxies in low density environments obtained using deep images from the Advanced Camera for Surveys (ACS) on the Hubble Space Telescope (HST). Using the ACS Virgo Cluster Survey as a counterpart in high-density environments, we investigate the role of environment in determining the properties of their globular cluster systems. We detect a strong colour bimodality of globular cluster systems in half of our galaxy sample. It is found that there is a strong correlation between the colour and richness of globular cluster populations and their host galaxy luminosities: the less bright galaxies possess bluer and fewer globular clusters as also seen in rich cluster environments. However, the mean colour of globular clusters in our field sample are slightly bluer than those in cluster environments at a given galaxy luminosity, and the colour of the red population has a steeper slope with absolute luminosity. By employing the YEPS simple stellar population model, the colour offset corresponds to metallicity difference of $\Delta$[F e/H ] ~ 0.15 - 1.20 or an age difference of $\Delta$age ~ 2 Gyr on average, implying that GCs in field galaxies appear to be either less metal-rich or younger than those in cluster galaxies. Although we have found that galaxy environment has a subtle effect on the formation and metal enrichment of GC systems, host galaxy mass is the primary factor that determines the stellar populations of GCs and the galaxy itself.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.215-215
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• 2022

Starch synthase proteins (SSI, SSII and SSIII) in rice are mainly involved in amylopectin synthesis mediating its chain elongation, and the functional loss of SSII can increase amylose accumulation through decreasing of amylopectin chain proportions. For purposes of identifying functional haplotypes and evolutionary analyses of this gene, SSII-1, we investigated 374 rice accessions belonging to different subgroups of origins. We subsequently performed bioinformatic analyses on their variations through haplotyping, resequencing and structuring based on different classified populations. Haplotyping of cultivated rice accessions using genetic variations within SSII-1 genomic region of chromosome 10 revealed a total of 8 haplotypes, representing 6 functional haplotypes by 4 non-synonymous SNPs of three different exons (1, 4 and 10), which effect on protein structure. Higher nucleotide diversity value was found in wild group (0.0055) compared to any of cultivated subpopulations, of which aus showed the most reduction of diversity value (0.0003). Tajima's D analysis exhibits the most Tajima's D value only in admixture group (0.3600) which appears to be the cause of a sudden population contraction by rare alleles scarcity. A clear separation of some wild accessions from the admixed cultivated subpopulations was observed in PCA and phylogenetic analysis. Similar admixed pattern of population structure was estimated with an increased K values of 2 to 8 where genetic components of almost all cultivated subpopulations were shared with the wild which can also be subsequently estimated by very low F_ST-values by -0.011 (wild-aromatic) and -0.003 (wild-admixture).

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.217-217
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• 2022

Pullulanase (PUL), a debranching enzyme, has been utilized in hydrolyzing the a-1,6 glucosidic linkages in starch, amylopectin, pullulan, as well as related oligosaccharides. It has also been indicated that PUL is a novel indicator of inherent RS (Resistant Starch) formation in rice. In this study, we performed haplotype analysis on 320 bred rice accessions, and additional 54 wild accessions were added to study genetic diversity along with other population-based analyses of the PUL gene. Through these investigations, we summarized a total of 10 functional (non-synonymous) SNPs from 7 different exons on chromosome 4. There were 10 haplotypes, of which only six haplotypes were functional, implicating different subpopulations. Diversity reduction was noticed in temperate japonica (0.0005) compared to the highest one (aus, 0.0154), illustrating their higher genetic differentiation by F_ST-value (0.926). The highest Tajima^ D value was observed in indica (3.6613), indicating PUL gene domestication signature under balancing selection, while the lowest Tajima's D value was found in temperate japonica (-2.2191) which might have undergone under positive selection and purified due to the excess of rare alleles. PCA, population structure, and phylogenetic analyses provide information on the genetic relatedness between and or among the cultivated subpopulations and the wild based on PUL genomic region.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.68-78
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• 1998

To investigate the genetic polymorphisms and constitutions of blood proteins and enzymes in the Korean native cattle population of National Livestock Cooperatives Federation(NLCF), the genetic variants of transferrin(Tf), post-transferrin-2(pTf-2), albumin(Alb), post-albumin(pAlb), ceruloplasmin(Cp), amylase-I(Am-I) and herroglobin(Hb) were analyzed using the PAGE(polyacrylamide gel electrophoresis) and ST AGE(starch gel electrophoresis) methods. On the genetic variants of the serum proteins, the transferrin(Tf) locus was assumed to be genetically controlled by codominant alleles, Tf A, $D_1$, $D_2$ and E alleles, and the gene frequencies of these were 0.249, 0.248, 0.260 and 0.243, respectively. The post-transferrin locus was observed to be controlled by pTf-2 F and S alleles, and the gene frequencies of these were 0.662 and 0.338, respectively. The post-albumin(pAlb) loci were identified to be controlled by two alleles, pAlb F and S alleles for pAlb locus, and the gene frequncies of these were 0.440 and 0.560 for pAlb F and S alleles, respectively. On the genetic variants of the serum enzymes, ceruloplasmin(Cp) and amylase-I(Am-I) loci were found to be controlled by two alleles, Cp F and S for Cp locus, and Am-I B and C for Am-I locus, and gene frequencies of these were 0.319 and 0.681 for Cp F and S, and 0.871 and 0.120 for Am-I Band C, respectively. On the genetic variants of the hemoglobin(Hb), the distributions of genotypes were 76.5, 21.2 and 2.3% for Hb AA, AB and BB types, and the gene frequnecies for Hb A and B were 0.871 and 0.129, respectively. On the effects of genetic variants of blood proteins, Tf $D_1D_1$, $D_2D_2$ and $D_2E$ genotypes were significantly higher on body weight at 6 month and average daily gain than that of other Tf genotypes.

• Journal of the Korean Chemical Society
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• v.29 no.4
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• pp.356-364
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• 1985

Ab initio calculations with STO-3G method were carried out on isomers of isobutylene dianion and dilithioisobutylene, and geometry, energy and Mulliken population were discussed. Energy of reaction, ${\Delta}$E, for isodesmic processes involving these species and relative heats of formation, ${\Delta}H_f$, estimated with ${\Delta}$E have shown that the contribution of "Y-aromaticity" to the structural stabilization of Y-type dianion is a tenuous one but the alkylation appears to proceed via the Y-type dilithio compound.

• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.708-716
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• 2020

The purpose of this study was to identify strawberry wilt pathogens and evaluate the efficacy of Chlorella fusca CHK0059 for improving plant growth and suppressing Fusarium wilt. We identified 10 isolates of wilt pathogens of non-pesticide Seolhyang strawberry plant, including Fusarium oxysporum f. sp. fragariae, using morphological and molecular analysis. On the 15^th day after 0.4% CHK0059 treatment, the plant height of the untreated control strawberry plants was significantly greater than that of the CHK0059-treated strawberry plants. After 85 days, both treatments showed a similar tendency regarding the height of the strawberry plants. However, the thickness of strawberry leaves treated with the CHK0059 was found to be 1 mm thicker than that of the untreated control. The flowering percentage of the CHK0059 plants was also 40.2% higher on average than that of the untreated control. The chlorophyll content of strawberry leaves treated with the CHK0059 was also, on average, 6.63% higher than that of the untreated control. After 90 days of the CHK0059 treatment, the incidence of Fusarium wilt in the CHK0059-treated plants had reduced by 9.8% on average compared to the untreated control. The population density of F. oxysporum f. sp. fragariae was also reduced by approximately 86.8% in the CHK0059-treated plants by comparison to the untreated control at 70 days after treatment. The results indicate that the microalga C. fusca CHK0059 is an efficient biological agent for improving strawberry plant growth and suppressing Fusarium wilt disease in organic strawberries.

• Safety and Health at Work
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• v.8 no.1
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• pp.105-115
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• 2017

Background: The goal of this study is to develop a general population job-exposure matrix (GPJEM) on asbestos to estimate occupational asbestos exposure levels in the Republic of Korea. Methods: Three Korean domestic quantitative exposure datasets collected from 1984 to 2008 were used to build the GPJEM. Exposure groups in collected data were reclassified based on the current Korean Standard Industrial Classification ($9^{th}$ edition) and the Korean Standard Classification of Occupations code ($6^{th}$ edition) that is in accordance to international standards. All of the exposure levels were expressed by weighted arithmetic mean (WAM) and minimum and maximum concentrations. Results: Based on the established GPJEM, the 112 exposure groups could be reclassified into 86 industries and 74 occupations. In the 1980s, the highest exposure levels were estimated in "knitting and weaving machine operators" with a WAM concentration of 7.48 fibers/mL (f/mL); in the 1990s, "plastic products production machine operators" with 5.12 f/mL, and in the 2000s "detergents production machine operators" handling talc containing asbestos with 2.45 f/mL. Of the 112 exposure groups, 44 groups had higher WAM concentrations than the Korean occupational exposure limit of 0.1 f/mL. Conclusion: The newly constructed GPJEM which is generated from actual domestic quantitative exposure data could be useful in evaluating historical exposure levels to asbestos and could contribute to improved prediction of asbestos-related diseases among Koreans.

• Journal of Korean Academy of Nursing
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• v.22 no.4
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• pp.552-568
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• 1992

Recent improvements in the standard of living, national income and medical care, and a decline in the infant death rate which have occurred related to economic growth and modernization, have led to a longer average life-span and a higher ratio of elderly people in the total population, Therefore, not only in the field of nursing science, but also in the field of many other discipline, issues concerning the elderly have been given increasing interest. A great deal of effort has been spent on increasing the quality of life for elderly people. This study was conducted to analyze the correlation between social support and quality of life. The sample consisted of III subjects residing at home and 107 subjects residing in institutions all of whom were over 65 years of age. The data collection period was from October 23, 1990 to January 26, 1991. Social support was measured using the Norbeck social Support Questionnaire developed by Norbeck, translated by Oh, Ka Sil and quality of life was measured using the QOL scale developed by No, You Ja. Data were analyzed using pereentages, t-test, Pearson Correlation Coefficient and ANOVA. The results of this study are as follows : 1. There was a statistically signifivant difference in the level of social support between the two groups (t＝-8.83, p＜.001), The elderly at home reported a much higher level of social support. 2. There was a statistically significant difference in the level of QOL between the two groups (t＝-5.77, p＜.001) . The elderly at home reported a much more positive quality of life. 3. There was a positive correlation between social support and QOL for the elderly at home ard it was statistically significant (r＝.32, p＜.001). 4. There was a postitive collealtion between social support and QOL for the elderly in institutions and it was also statistically significant (r＝.19, p＜.05). 5. The relationship between the general characteristics of the elderly at home and the variables of social support and of QOL were as follows ; 1) according to sex(t＝10.57, p＜.01) and the number of offspring(F＝6.19, p＜.01), there was a statistically significant difference in social support. 2) according to amount of Pocket money, there was a statistically significant difference in QOL(F＝2.98, p＜.05). 6. The relationship between the general characteristics of the elderly in institutions and the two variables were as follows ; 1) according to sex(t＝6.24, p＜.05), the number of offspring(F＝6.16, p＜.001) and religion (F＝2.58, p＜.05), there was a statistically significant difference in social support. 2) according to religion, there was a statistically significant difference in QOL(F＝2.90, p＜.05). In conclusion, it can be said that social support is an important variable related to QOL in the elderly and that social support levels are higher for the elderly residing at home. Therefore, more specific and objective approaches and efforts are needed to effectively use resources to maintain the elderly at home and to enhance social support available to the elderly in institutions and thereby increase QOL regardless of residence.