• The Plant Pathology Journal
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• 제31권2호
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• pp.140-151
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• 2015

Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (Foc). The population dynamics of Foc, strain B006 and its surfactin over-producing mutant B841 and surfactin-deficient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing Foc. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foc propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foc population at the cucumber stems in a range of $0.96log_{10}ng/g$ to $2.39log_{10}ng/g$ was attained at the third and the fifth weeks of B006 treatment in nursery substrate. In field-infected soil, all three treatments with B. subtilis suppressed Foc infection, indicated by the reduction of Foc population at a range of $2.91log_{10}ng/g$ to $3.36log_{10}ng/g$ at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application as biocontrol agent in the field.

• Journal of the korean society of oceanography
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• 제32권2호
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• pp.85-92
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• 1997

Seasonality in abundance and egg production was investigated for Calanus sinicus in Asan Bay, Korea, and feeding effort was measured in the laboratory condition. Although abundances of this species in this bay showed only one Peak in spring, egg Production showed two peaks, spring and fall, in this bay. Potential of year-round egg Production was also found. Food availability judged by the particulate organic carbon (POC) concentration was usually more than enough in this bay. High egg Production in fall resulted in spring Peak in abundance. But high egg Production in spring did not result in summer or fall Peak in abundance. Low abundances in summer and fall were believed to be the result of long range horizontal migration out to the central Part of the Yellow Sea. The highest average egg Production by the Population of this species was 16.3 eggs female-'day-'. Maximum e99 Production by an individual was 39.0 e99s female$^{-1}$ day$^{-1}$, which yielded 30.7% of specific egg Production rate in terms of body carbon content with the carbon contents of 44.44$^{\mu}$gC and 0.35 $^{\mu}$gC for individual adult female and egg, respectively. Clearance rate (F) measured in the laboratory condition decreased exponentially with the increase of food concentrations, as expected. The asymptote of F was 3.17 ml copepod$^{-1}$ h$^{-1}$. When this value and the minimum concentration of POC measured in the field were applied under the assumption of continuous feeding in the nature, the calculated gross efficient of egg production was 41.6% in case of average egg production of the population. Violation of the assumption of diurnal feeding rhythm and application of the minimum concentration of POC may offset the influence on calculation each other.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1536-1540
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• 2006

Crossbreeding effects for wool quality traits viz. greasy fleece weight (kg), staple length (cm), average fibre diameter (${\mu}$) and medulation percentage were estimated using the Dickerson's and Kinghorn's models. The data analyzed involved 15 genetic groups including Nali purebred, $F_1$'s of two and three breeds, $F_2$'s and reciprocal crossbred obtained from the crossing of Nali (N), Merino (M) and Corriedale (C) breeds during 1980-96. Nali and Corriedale breeds had non-significant negative additive genetic effects (Dickerson's model) on greasy fleece weight, while effects of Corriedale were negative for staple length only from both models. In general additive genetic effects of all three breeds were non-significant for all the wool traits except medulation percentage. Non significant heterotic and recombination effects (epistatic loss) were estimated from both models. However, the estimates of crossbreeding effects varied between the models both in magnitude as well as in direction barring few exceptions. Undesirable positive heterosis was found on medulation percentage for all types of combinations involving three breeds. Comparison of least squares means of various genetic groups revealed that both two breed and three breed crosses were superior to the Nali breed for all wool quality traits. Fibre diameter of MN crossbreds was significantly less than CN crossbreds. Results also indicated that as the inheritance of Nali breed in a cross is decreased, the medulation percentage decreases which is desirable. Inter se mating of crossbreds (two breed, three breed) has not resulted in a decline in the wool quality traits. These results indicate that the synthetic population derived from three breeds can be stabilized easily for wool traits as there may not be epistatic loss on subsequent inter se mating of crossbreds.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.327-332
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• 2016

The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average $F_{is}$ value for the breeds ranged from -0.044 (ETH225) to 0.180 (SPS115), while the estimated average $F_{st}$ value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy.

• 한국산림과학회지
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• 제97권2호
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• pp.187-195
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• 2008

Picea jezoensis (Sieb. et Zucc.) Carriere is one of the major and widespread components of the cold-temperate and boreal forests in Russian Far East, northeast China, Korea, and Japan. However, it is restricted to a highly fragmented range in South Korea with small populations. Mean expected heterozygosity $(H_e)$ based on 22 loci in 11 isozyme systems was 0.077 for four sampled populations that covered the whole distribution range of P. jezoensis in South Korea. This value is within the range reported for conifers, but it is very low compared to that of other spruce species as well as that of P. jezoensis populations in Russian Far East. Most populations had a slight excess of heterozygotes and the Wright's $F_{IS}$ (-0.019) was comparable to that previously reported for other spruce species. In all of the four populations, the Wilcoxon sign-rank test indicated no greater heterozygosity than that expected for populations at mutation-drift equilibrium, suggesting that the populations have not been bottlenecked recently. Despite a fragmented range and isolated populations, population differentiation was not high $(F_{ST}=0.047)$ and the number of migrants per generation was 5.09. Nei's genetic distances were also small $({\bar{D}}=0.005)$ but strongly related to geographic distances between populations, suggesting an Isolation by Distance. The northernmost isolate, Mt. Gyebang population was genetically distinct from the other three populations. Implications for the conservation of genetic variation of P. jezoensis in South Korea were discussed.

• 한국응용곤충학회지
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• 제50권1호
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• pp.21-27
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• 2011

2005년부터 2007년까지 경남 진주, 하동지역 매실에서 25종의 해충이 조사되었다. 붉은테두리진딧물(Rhopalosiphum rufiabdominale(Sasaki)), 외점애매미충(Singapora shinshana M.), 벚나무응애(Tetranychus viennensis Zacher), 복숭아유리나방(Synanthedon hector B.) 등이 주요해충 이었으며, 복숭아꽃바구미(Anthonomus persicae sp. nov.), 복숭아순나방(Grapholita molesta (Busk)), 담배거세미나방(Spodoptera litura F.), 복숭아혹진딧물(Myzus persicae) 등이 일시적으로 발생하는 해충으로 나타났다. 외점애매미충의 발생은 4월 하순부터 10월 하순까지 지속적으로 발생하였고, 벚나무응애는 5월초에 발생하기 시작하여 6월 하순에서 7월 상순사이에 정점에 도달한 후 잎의 섭식조건 악화와 더불어 밀도가 감소하였다. 복숭아유리나방은 6월 중순 뚜렷한 발생최성기를 보인 후 7월 상순부터 8월 상순사이 기간에 발생이 거의 없었다가 9월 초중순에 뚜렷한 2차 발생최성기를 보였다. 복숭아꽃바구미는 매실 개화 전 꽃눈에서 발생하였고, 그 이후 4월~5월초에 우화 성충이 관찰되었다.

• 한국약용작물학회지
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• 제25권4호
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• pp.224-230
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• 2017

Background: The current regulations regarding the storage of medicinal herbs do not define the storage temperature; instead, but the only requirement is that the plastic bags used for storing the medicinal herbs should be tightly sealed during storage. The practice of storing medicinal herbs at room temperature ($28^{\circ}C$), causes many problems, including growth of insect pests on the stored medicinal herbs. Thus, it is necessary to understand the effect of insect pests on the quality of medicinal herbs stored at room temperature ($28^{\circ}C$) for the improving the relevant regulations. Methods and Results: Cnidium rhizome and Angelica radix were infested with Lasioderma serricorne F. adults and incubated at $28^{\circ}C$ for 2.5 and 5 months. The population of L. serricorne was established rapidly, and left many holes, cadavers, and feces on the stored medicinal herbs, thereby greatly damaging the product appearance and hygiene. In addition, active ingredients of the medicinal herbs were significantly decreased, probably reducing their medicinal quality as well. Conclusions: These results indicate that the current regulations are not sufficient to prevent the establishment of storage insect pests and guarantee the quality and hygiene of stored medicinal herbs. Therefore, it is necessary to devise proper storage protocols and upgrade the current relevant regulations to maintain the quality of medicinal herbs during storage.

• 한국인구학
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• 제11권1호
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• pp.87-96
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• 1988

The subjects used in this study were chosen from self-employed women in Taegu City, Korea, Data for 415 respondents were collected by face-to-face interviews conducted during the period of November 10 through 23, 1987. There are three major purposes in this study : first, to describe the general characteristics of self-employed Korean women's fertility behavior ; second, to examine the determinants of fertility behavior among self-employed women : third, to explore the relative importance of the fertility determinants among the women. Regression analyses were introduced to test hypotheses. Nine variables, such as household income, educational attainment, mass media, abortion, the period of contraception practice, contraceptive expenses, the number of infant, deaths marital period, and age at first marriage were introduced as indicators in the regression. The results of this study show that there are negative relationships between the dependent variable of fertility and abortion, and the period of contraception practice. On the other hand, positive relationships between the dependent variable of fertility and the number of infant deaths, and marital period and age at first marriage are shown in this study. However, the results of this study show that there are insignificant relationships between socioeconomic variables and fertility. From the results of the F test to evaluate the significance of difference in $R^2$between two different regression equations, we have found that the regression equation including both socioeconomic variables and intermediate variables is misspecified to examine the determinants of fertility behavior among self-employed Korean women. That is, the F test shows that the regression equation including only intermediate variables as indicators is the best model for this study. Finally, the relative importance of the fertility determinants among self-employed women is found as follows : Age at the first marriage is the most influential variable in the fertility, and the next important factors are abortion, the period of contraception practice, the number of infant deaths, and marital period, in that order.

• 한국의류학회지
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• 제34권10호
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• pp.1581-1595
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• 2010

This paper investigates the regional characteristics of street fashion in China. Yanji, Beijing, Shanghai were chosen as three different cities in terms of location, weather, population, and industrial structure. A total of 592 pictures were collected through an internet and fashion magazine search for street fashion in Beijing and Shanghai in addition photos were taken for those in Yanji. Pictures of each city were classified into groups based on overall images covering top, bottom, and accessories to identify the characteristics of style in each group. The classification process included 2 stages. In the first stage, it produced 2 groups: formal and casual. The second stage divided formal into business formal and retro formal; casual was divided into II sub-groups that were easy, sporty, feminine, sexy, ethnic, girlish, nippon, trendy, bulky, military, and mixed. Easy casual showed the highest frequency for 3 cities and military style showed the lowest. Shanghai showed higher frequency in sporty, trendy, and military style than other cities. Each style exposed the similarities and differences in the cities that reflected different regional characteristics.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.117-117
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• 2017

The onion (Allium cepa L.) is the most widely cultivated species of the genus Allium, especially it has been valued because of the pungent flavor and aroma. Allium species including onion has very large genome sizes ranging from approximately 10 to 20 Gbp, which have complicated genomic studies and precluded genome sequencing until recently. A population of 186 F2 individuals derived from a cross of 'Umjinara' ${\times}$ 'Sinsunhwang' and the two parental lines were used for this study. For the development of framework map, various types of markers including SSRs, RAPD, SNPs, and CAPS makers have been used for polymorphism test. Especially, a lot of SNP and CAPS loci were developed from the onion transcriptome sequence by RNASEQ of two parental lines. The GBS libraries have been constructed based on a modified protocol from Poland Lab using a two-enzyme system. We have been developing markers showing polymorphism between two parental lines, and genotyping for all F2 individuals were finished for a number of polymorphic markers. For the construction of GBS libraries, a set of 192 barcoded adapters were generated from complementary oligonucleotides with XhoI overhang sequence and unique barcodes of length 4-8 bp and they have been tested using two parental linesto determine the optimum conditions for GBS analysis.