• Title/Summary/Keyword: F2 population

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Inheritance of Scentedness of Rice Leaf in a Scented Breeding Line 'P-33-C-19' (향도의 방향성 유전)

  • 박순직
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.1
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    • pp.11-14
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    • 1984
  • The inheritance of scent in rice leaf and its genetic association with base color, hull color and resistance to bacterial leaf blight were studied in F$_2$ population of two rice crosses, P-33-C-19 (scented)/Zhu-Lian-Ai and P-33-C-19/Kataktara DA2. The scent was identified by leaf analysis at tillering stage. The scented and non-scented plants segregated in the ratio of 1scented: 3non-scented showing that a recessive gene responsible for the expression of scentedness. The scentedness was independently segregated with base color. hull color and resistance to bacterial leaf blight.

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Study on the Relationship between Utilization of Nutrients in the Rumen and Rumen Microorganisms by Dietary Energy and Protein Source (에너지와 단백질(蛋白質) 공급원(供給源)에 의(依)한 반추위내(反芻胃內) 영양소(營養素) 이용성(利用性) 및 반추위미생물(反芻胃微生物)에 관(關)한 연구(硏究))

  • Chee, Sul Ha;Lee, Sang Cheol;Jeong, Ha Yeon
    • Korean Journal of Agricultural Science
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    • v.23 no.2
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    • pp.188-205
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    • 1996
  • This study was conducted to examine the effects of dietary energy and protein sources on the rumen microbial population of sheep. The results obtained were summarized as the follows; 1. Ruminal pH was highest in sheep fed the diet rapidly degraded in the rumen(F-F) as a energy (barley plus beet pulp) and protein source (rapeseed meal), and lowest in the diet (F-S) of rapidly degradable energy source plus slowly degradable protein source (corn gluten meal + cotton seed meal) without affecting by postfeeding time. 2. Ruminal ammonia concentration was higher in corn (slowly degradable) for energy source and rapeseed meal (rapidly degradable) for protein source (S-F) than others, and abruptly increased at 1 hr after feeding regardless of treatments. 3. Concentration of ruminal total volatile fatty acid, acetate and propionate were highest in F-S and peaked at 1 hr after feeding firstly and formed second peak at 9hrs, respectively. 4. Digestibilities of the proximates and ADF were not affected by treatment but NDF was highest in F- F. 5. Nitrogen retention was highest in S-F and lowest in F-F and F-S. 6. Digestibility and metabolizability of energy, contents of OCP, TDN, DE and ME were not affected by treatment.

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Population ecology of Palmaria palmata (Palmariales, Rhodophyta) from harvested and non-harvested shores on Digby Neck, Nova Scotia, Canada

  • Garbary, David J.;Beveridge, Leah F.;Flynn, Andrea D.;White, Katelyn L.
    • ALGAE
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    • v.27 no.1
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    • pp.33-42
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    • 2012
  • Population ecology of Palmaria palmata is described from the intertidal zone of Digby Neck and adjacent islands of Nova Scotia. The primary objectives were: to evaluate the difference in habitat specialization and population structure of P. palmata between harvest and non-harvest shores, and to characterize differences in thallus structure and frond sizes between epilithic and epiphytic populations. Harvest shores were gently sloping boulder fields with boulders typically about 0.5-1.0 m with dense cover of P. palmata on many of the rocks. Non-harvest shores (with or without P. palmata) consisted of boulders that were smaller or larger than harvest shores, or bedrock; when P. palmata was present on nonharvest sites it was typically epiphytic on other algae (e.g., Fucus spp., Mastocarpus stellatus, Devaleraea ramentacea). Harvestable epiphytic populations occurred only in high current areas. While there was little difference in average cover of P. palmata harvest and non-harvest shores ($31.2{\pm}13.7%$ vs. $19.4{\pm}7.3%$, mean ${\pm}$ standard deviation [SD]), the cover of P. palmata on harvest shores was highly skewed such that individual boulders often had >90% cover while adjacent rocks had little. Frond length of large fronds was greater on harvested shores, and mean frond density ($g\;m^{-2}$) was three times higher than the mean density on the non-harvested shores. Frond lengths of entire epiphytic and epilithic frond complements of 119 thalli from harvest beaches showed no difference in mean size of the largest fronds, and no difference in frond number per holdfast when epiphytic and epilithic thalli were compared.

Genetic Diversity and Population Structure of Chimaphila japonica in Southern Part of Korea (한국 남부지역의 매화노루발의 유전적 다양성과 집단구조)

  • Joo-Soo Choi;Man-Kyu Huh
    • Journal of Life Science
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    • v.8 no.6
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    • pp.687-694
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    • 1998
  • Enzyme electrophoresis was used to estimate genetic diversity and population structure of Chimaphila japonica Miq. in Korea. The percent of polymorphic loci within the enzymes was 48.7%. Genetic diversity at the species level and at the population level was high (Hes=0.278 ; Hep=0.222, respectively), whereas the extent of the population divergence was relatively low ( $G_{ST}$ =0.079). $F_{IS}$ , a measure of the deviation from random mating within the 7 populations, was 0.355. An indirect estimate of the number of migrants per generation (Nm=2.61) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Factors contributing to the high levels of genetic dive-rsity found in the entire species of C. japonica include wide distribution, long-lived perennials, ability to regenerate due to rhizomatous spread, outcrossing induced by animal vectors, and occasional pollen dispersal by wind.

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Independent Inheritance between df2 gene and ti gene in Soybean

  • Han, Eun-Hui;Sung, Mi-Kyung;Kim, Kyung-Roc;Park, Jung-Soo;Nam, Jin-Woo;Chung, Jong-Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.14-17
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    • 2011
  • Dwarfuess and Kunitz trypsin inhibitor (KTI) protein in soybean is useful traits for basic studies. df2 and ti gene control dwarfness and the expression of Kunitz trypsin inhibitor (KTI) protein in soybean, respectively. The objective of this research was to verify genetic linkage or independent inheritance of df2 and ti loci in soybean. The $F_2$ population was made by cross combination between "Gaechuck#2" (Df2Df2titi genotype, KTI protein absence and a normal growth type) and T210 (df2df2TiTi genotype, a dwarf growth type and KTI protein present). A total of 258 $F_2$ seeds were analyzed for the segregation of KTI protein using SDS-PAGE. And so, 198 $F_2$ plants were recorded for the segregation of dwarfness. The segregation ratio of 3 : 1 for Ti locus (201 Ti_ : 57 titi) and Df2 locus (143 Df2_ : 55 df2df2) was observed. Segregation ratio of 9 : 3 : 3 : 1 (116 Ti_Df2_: 44 Ti_df2df2: 27 titiDf2_: 11 titidf2df2) between df2 gene and ti gene was observed ($x^2$=3.53, P = 0.223). These results showed that df2 gene was inherited independently with the ti gene in soybean.

Enrollment in Private Medical Insurance and Utilization of Medical Services Among Children and Adolescents: Data From the 2009-2012 Korea Health Panel Surveys

  • Ryu, Dong Hee;Kam, Sin;Doo, Young-Taek
    • Journal of Preventive Medicine and Public Health
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    • v.49 no.2
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    • pp.118-128
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    • 2016
  • Objectives: The purposes of this study were to examine the status of children and adolescents with regard to enrollment in private medical insurance (PMI) and to investigate its influence on their utilization of medical services. Methods: The present study assessed 2973 subjects younger than 19 years of age who participated in five consecutive Korea Health Panel surveys from 2009 to 2012. Results: At the initial assessment, less than 20% of the study population had not enrolled in any PMI program, but this proportion decreased over time. Additionally, the number of subjects with more than two policies increased, the proportions of holders of indemnity-type only ('I'-only) and of fixed amount+indemnity-type ('F+I') increased, whereas the proportion of holders with fixed amount-type only ('F'-only) decreased. Compared with subjects without private insurance, PMI policyholders were more likely to use outpatient and emergency services, and the number of policies was proportionately related to inpatient service utilization. Regarding outpatient care, subjects with 'F'-only PMI used these services more often than did uninsured subjects (odds ratio [OR], 1.69), whereas subjects with 'I'-only PMI or 'F+I' PMI utilized a broad range of inpatient, outpatient, and emergency services relative to uninsured subjects (ORs for 'I'-only: 1.39, 1.63, and 1.38, respectively; ORs for 'F+I': 1.67, 2.09, and 1.37, respectively). Conclusions: The findings suggest public policy approaches to standardizing PMI contracts, reform in calculation of premiums in PMI, re-examination regarding indemnity insurance products, and mutual control mechanisms to mediate between national health insurance services and private insurers are required.

Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence

  • Hong, Sung Won;Kim, Da-Ran;Kim, Ji Su;Cho, Gyeongjun;Jeon, Chang Wook;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • v.34 no.3
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    • pp.163-170
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    • 2018
  • Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in soil. Development of detection and quantification techniques are regarded significantly in many soilborne pathogens to prevent damage from diseases. In this study, we improved specific-quantitative primers for F. oxysporum f. sp. fragariae to reveal correlation between the pathogen density and the disease severity. Standard curve $r^2$ value of the specific-quantitative primers for qRT-PCR and meting curve were over 0.99 and $80.5^{\circ}C$, respectively. Over pathogen $10^5cfu/g$ of soil was required to cause the disease in both lab and field conditions. With the minimum density to develop the wilt disease, the pathogen affected near 60% in nursery plantation. A biological control microbe agent and soil solarization reduced the pathogen population 2-fold and 1.5-fold in soil, respectively. The developed F. oxysporum f. sp. fragariae specific qRT-PCR protocol may contribute to evaluating soil healthiness and appropriate decision making to control the disease.

Mapping QTL for Grain Quality Traits Using an Introgression Line Population from a Cross between Ilpumbyeo and Moroberekan in Rice (일품벼/모로베레칸 이입계통을 이용한 미질특성 관련 QTL 분석)

  • Ju, Hong-Guang;Kim, Dong-Min;Oh, Chang-Sik;Kim, Myung-Ki;Kim, Kee-Jong;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.429-436
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    • 2009
  • We conducted a QTL analysis of grain quality traits using 117 $BC_3F_4$ and $BC_3F_5$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_4$ and $BC_3F_5$ lines were evaluated for eleven grain quality traits in 2005 and 2006. A total of 18 QTLs were identified for eleven traits, and the phenotypic variance explained by each QTL ranged from 9.9% to 35.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at two QTL loci for 1,000 grain weight. Four QTLs, two for chalky rice and one each for 1,000 grain weight and head rice were consistently detected in two consecutive years indicating that these QTLs are stable. Clusters of QTLs were observed in three chromosome regions. One cluster harboring five QTLs including head rice and brown rice ratio near SSR markers RM190 and RM314 was detected on chromosome 6. Another cluster harboring grain weight and white belly was detected on chromosome 2. Increase in white belly at this locus might be due to the increase in grain weight due to the presence of the Moroberekan allele. The Moroberekan alleles at two QTL loci, gw3 and gw4 associated with increased grain weight might be useful in breeding programs to develop high-yielding cultivars.

An Analytical Study on Health-Promoting Lifestyle Patterns and Associated Variables of Korean Immigrant Elderly in Seattle

  • Sohng, Kyeong-Yae;Yeom, Hye-A
    • Journal of Korean Academy of Nursing
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    • v.29 no.5
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    • pp.1058-1071
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    • 1999
  • Although healthy lifestyles have been proved as an effective way of improving higher well-beings for individuals. researches on health-promoting behaviors of minority elderly with a specific ethnic heritage have been sparsely tried. This study was designed to explore health-promoting lifestyle patterns of Korean immigrant elderly living in Seattle, USA and its relationships with two associated perceptual variables, self-esteem and perceived health status. One hundred ten Korean immigrant elderly were recruited from two senior centers and interviewed with a structured questionnaire. Data were collected from October 1998 to January 1999, and analyzed using SPSS program through which t-test, ANOVA, and Pearson Correlation Coefficients were tested. As the results, the mean HPLP score of the Korean immigrant elderly was 2.54 (SD = .36), showing significant differences by education (F = 3.61, P = .016), economic status (F = 3.01, P = .034), and current health status (F = 3.69, p = .008). In self-esteem, two socioeconomic variables showed statistical association with self-esteem : marital status (t = 2.47, P = .015) and living situation (F = 4.03, p = .021). The HPLP subscales that showed higher mean scores were nutrition (M = 3.01, SD = .52) and interpersonal support (M = 2.65, SD = .47) while lower mean scores were detected in the domain of exercise (M = 1.92, SD = .74) and stress management (M = 2.26, SD = .47). Perceived health status revealed significant positive correlation with health-promoting lifestyle patterns (r = .19, P = .043) and self-esteem (r = .32, P = .001) in the present study. It is concluded that engagement in health-promoting lifestyle patterns should be actively encouraged to enhance personal health of Korean immigrant elderly. Developing health promotion programs focused on exercise and stress management is also imperatively suggested not only for better health practices of Korean immigrant elderly population but also for enhancing their level of well-beings and life satisfaction.

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RNA Expression of Cytochrome P450 in Mexican Women with Breast Cancer

  • Bandala, Cindy;Floriano-Sanchez, E.;Cardenas-Rodriguez, N.;Lopez-Cruz, J.;Lara-Padilla, E.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.6
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    • pp.2647-2653
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    • 2012
  • Involvement of cytochrome P450 genes (CYPs) in breast cancer (BCa) may differ between populations, with expression patterns affected by tumorigenesis. This may have an important role in the metabolism of anticancer drugs and in the progression of cancer. The aim of this study was to determine the mRNA expression patterns of four cytochrome P450 genes (CYP2W1, 3A5, 4F11 and 8A1) in Mexican women with breast cancer. Real-time PCR analyses were conducted on 32 sets of human breast tumors and adjacent non-tumor tissues, as well as 20 normal breast tissues. Expression levels were tested for association with clinical and pathological data of patients. We found higher gene expression of CYP2W1, CYP3A5, CYP4F11 in BCa than in adjacent tissues and only low in normal mammary glands in our Mexican population while CYP8A1 was only expressed in BCa and adjacent tissues. We found that Ki67 protein expression was associated with clinicopathological features as well as with CYP2W1, CYP4F11 and CYP8A1 but not with CYP3A5. The results indicated that breast cancer tissues may be better able to metabolize carcinogens and other xenobiotics to active species than normal or adjacent non-tumor tissues.