• 제목/요약/키워드: F.E. analysis

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Functional Analysis of the marB gene of Escherichia coli K-12

  • Lee, Chang-Mi;Park, Byung-Tae
    • 대한의생명과학회지
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    • 제10권2호
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    • pp.153-161
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    • 2004
  • Antibiotic resistance is often associated with the production of inner membrane proteins (for example, AcrAB/TolC efflux pump) that are capable to extrude antibiotics, detergents, dyes and organic solvents. In order to evaluate the unknown MarB function of Escherichia coli, especially focused on the function of OmpF porin, several mutants were construted by T4GT7 transduction. MarA plays a major roles in mar (multiple antibiotic resistance) phenotype with AcrAB/TolC efflux pump in E. coli K-12. Futhermore, MarA decreases OmpF porin expression via micF antisense RNA. Expression of acrAB is increased in strains containing mutation in marR, and in those carrying multicopy plasmid expressing marA. MarB protein of E. coli K-12 showed its activity at OmpF porin & TolC protein as target molecule. Some paper reported MarB positively regulates OmpF function. MarA shows mar phenotype, and MarB along with MarA show decreased MIC through OmpF function. By this experiment, MarB could decrease MIC through the OmpF porin & TolC protein as target.

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Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

  • Son, OGyeong;Yoon, Chang Young;Park, SeonJoo
    • 한국자원식물학회지
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    • 제27권6호
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    • pp.671-679
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    • 2014
  • Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.

Hybrid III 5% 성인 여성 더미의 유한요소 모델 개발 (Development of Finite Element Model of Hybrid III 5th Percentile Female Dummy)

  • 이상일;;;박경진
    • 한국자동차공학회논문집
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    • 제18권4호
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    • pp.18-30
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    • 2010
  • As the automobile industry is developing, the number of deaths and injuries has increased. To reduce the damages from automobile accidents, the government of each country proposes experimental conditions for reproducing the accident and establishes the vehicle safety regulations. Automotive manufacturers are trying to make safer vehicles by satisfying the requirements. The Hybrid III crash test dummy is a standard Anthropomorphic Test Device (ATD) used for measuring the occupant's injuries in a frontal impact test. Since a real crash test using a vehicle is fairly expensive, a computer simulation using the Finite Element Method (F.E.M.) is widely used. Therefore, a detailed and robust F.E. dummy model is needed to acquire more accurate occupant injury data and behavior during the crash test. To achieve this goal, a detailed F.E. model of the Hybrid III 5th percentile female dummy is constructed by using the reverse engineering technique in this research. A modeling process is proposed to construct the F.E. model. The proposed modeling process starts from disassembling the physical dummy. Computer Aided Design (CAD) geometry data is constructed by three-dimensional (3-D) scanning of the disassembled physical dummy model. Based on the geometry data, finite elements of each part are generated. After mesh generation, each part is assembled with other parts using the joints and rigid connection elements. The developed F.E. model of dummy is simulated based on the FMVSS 572 validation regulations. The results of simulation are compared with the results of physical tests.

Parametric study of laterally loaded pile groups using simplified F.E. models

  • Chore, H.S.;Ingle, R.K.;Sawant, V.A.
    • Coupled systems mechanics
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    • 제1권1호
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    • pp.1-7
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    • 2012
  • The problem of laterally loaded piles is particularly a complex soil-structure interaction problem. The flexural stresses developed due to the combined action of axial load and bending moment must be evaluated in a realistic and rational manner for safe and economical design of pile foundation. The paper reports the finite element analysis of pile groups. For this purpose simplified models along the lines similar to that suggested by Desai et al. (1981) are used for idealizing various elements of the foundation system. The pile is idealized one dimensional beam element, pile cap as two dimensional plate element and the soil as independent closely spaced linearly elastic springs. The analysis takes into consideration the effect of interaction between pile cap and soil underlying it. The pile group is considered to have been embedded in cohesive soil. The parametric study is carried out to examine the effect of pile spacing, pile diameter, number of piles and arrangement of pile on the responses of pile group. The responses considered include the displacement at top of pile group and bending moment in piles. The results obtained using the simplified approach of the F.E. analysis are further compared with the results of the complete 3-D F.E. analysis published earlier and fair agreement is observed in the either result.

강진 삼흥리 가마 소토시료의 고고지자기 연대측정 (Archaeomagnetic Dating of Baked Earth Samples of Samheung-ri Kilns in Gangjin Region)

  • 성형미
    • 보존과학회지
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    • 제31권1호
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    • pp.29-35
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    • 2015
  • 전라남도 강진군 삼흥리에서 A~F의 6개 지구로 나누어 유적조사가 진행되었는데 청자가마와 도기가마로 이루어진 총 16기의 가마가 발견되었다. 그 중에서 A D E F지구에서 청자가마 3기와 도기가마 6기, 총 9기의 가마에 대해 소토시료를 채취하여 고고지자기 분석이 이루어졌다. 이 소토시료는 소성상태가 양호하며 토질도 좋은 편이어서 신뢰도가 높은 고고지자기 데이터를 구할 수가 있었다. 고고지자기 분석을 통해 A.D.990~1250년 사이에 해당하는 9기 가마 각각에 대한 고고지자기 연대를 구할 수 있었으며, 이러한 결과는 고려시대로 추정되는 고고학적 연대와 일치한다. 그리고 지구별로 가마들의 조업시기에 차이가 있는지에 대해서도 알아보았는데, D지구(A.D.1100년) ${\rightarrow}$ E F지구(A.D.1180년) ${\rightarrow}$ A지구(A.D.1210년)의 순서로 고고지자기 연대에서 시기 차이가 확인되었다. 이러한 결과를 통해 D지구의 가마가 가장 이른 시기에 조업이 끝나서 가마들이 폐기되었고 다음은 E와 F지구가 거의 동시기에, 그리고 마지막으로 A지구가 가장 늦은 시기까지 조업이 진행되다가 폐기된 것임을 알 수 있었다.

유한대판법(有限帶板法)에 의한 보강(補强)된 평판(平板)의 해석(解析) (Analysis of Stiffened Plate by Finite Strip Method)

  • 임상전;박병욱
    • 대한조선학회지
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    • 제16권3호
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    • pp.1-8
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    • 1979
  • Various approaches to the analysis of stiffened plating in ship structures have been proposed by a number of researchers. Among them, the finite element method is known to be the most powerful method. However, for many parts of ship's structure having simple geometry and boundary conditions, the F.E.M. is often extravagant and unnecessary. In this paper, the authors have attempted to introduce the finite strip method which was proposed by Y.K. Cheung to avoid the difficulties involved in F.E.M. The results of calculations on the displacements and stresses in various plates with or without stiffeners were satisfactory, which shows the F.S.M. is useful for structural analysis of ship's plating.

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슬롯피치 경계 조건을 이용한 인버터 구동 유도 전동기의 회전자 등가회로 정수 추출 및 특성해석 (Analysis of Inverter-fed Induction Motor using F.E.M & Equivalent Circuit)

  • 이건호;김병택;권병일;박승찬
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2000년도 하계학술대회 논문집 B
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    • pp.632-634
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    • 2000
  • This paper presents a simple analysis method for inverter-fed induction motor using F.S.M and equivalent circuit. This analysis is carried out to analyze an induction motor driven by the sinusoidal voltage and the inverter. The results are verified by comparing with those of the time-step F.E.A and the experiment.

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P-e 곡선의 타원 특성을 이용한 전력계통 최대허용부하의 예측 (Estimation of Maximum Loadability in Power Systems By Using Elliptic Properties of P-e Curve)

  • 문영현;최병곤;조병훈;이태식
    • 대한전기학회논문지:전력기술부문A
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    • 제48권1호
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    • pp.22-30
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    • 1999
  • This paper presents an efficient algorithm to estimate the maximum load level for heavily loaded power systems with the load-generation vector obtained by ELD (Economic Load Dispach) and/or short term load forecasting while utilizing the elliptic pattern of the P-e curve. It is well known the power flow equation in the rectangular corrdinate is jully quadratic. However, the coupling between e and f makes it difficult to take advantage of this quadratic characteristic. In this paper, the elliptic characteristics of P-e curve are illustrated and a simple technique is proposed to reflect the e-f coupling effects on the estimation of maximum loadability with theoretical analysis. An efficient estimation algorithm has been developed with the use of the elliptic properties of the P-e curve. The proposed algorithm is tested on IEEE 14 bus system, New England 39 bus system and IEEE 118 bus system, which shows that the maximum load level can be efficiently estimated with remarkable improvement in accuracy.

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위상이동 방법에 의한 다결정 $Ir/H_2SO_4$ 수성 전해질 계면에서 과전위 수소흡착에 관한 해석 (An Analysis on the Over-Potentially Deposited Hydrogen at the Polycrystalline $Ir/H_2SO_4$ Aqueous Electrolyte Interface Using the Phase-Shift Method)

  • 천장호;문경현
    • 전기화학회지
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    • 제3권2호
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    • pp.109-114
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    • 2000
  • 다결정 $Ir/H_2SO_4$수성 전해질 계면에서 중간주파수 구간의 위상이동 변화와 Langmuir흡착등온식 사이의 관계를 교류임피던스 방법 즉 위상이동 방법을 이용하여 연구 조사하였다. 간소화된 계면 등가회로는 전해질저항(Rs), Faraday저항$(R_F)$, 흡착유사용량$(C_\phi)$ 등가회로 요소$(C_P)$의 직렬접속으로 구성된다. 음전위(E)에 대한 위상이동$(-\phi)$과 표면피복율$(\theta)$ 변화율$[\Delta(-\phi)/{\Delta}E,\;{\Delta}{\theta}/{\Delta}E]$을 비교 및 제시하였다. 지연되는 위상이동$(-\phi)$은 음전위(E) 및 주파수(f)에 따르며, $\phi=tan^{-1}[1/2{\pi}f(R_s+R_F)C_P]$이다. 중간주파수(1 Hz)에서 위상이동 변화$(-\phi\;vs.\;E)$는 Langmuir흡착등온식 $(\theta\;vs.\;E)$의 결정에 적용할 수 있는 실험적인 방법이다. 다결정 Ir/0.1 M $H_2SO_4$ 전해질 계면에서 수소의 흡착평형상수(K)와 흡착표준자유에너지 $({\Delta}G_{ads})$는 각각 $2.0\times10^{-4}$와 21.1kJ/mol이며 과전위 수소흡착(OPD H)에 기인한다.

F-5E/F 외부 연료탱크 수평 핀 균열 분석 및 재설계 (The Crack Analysis and Redesign of Horizontal Fin of F-5E/F's External Fuel Tank)

  • 강치행;윤용인;정대한
    • 한국항공우주학회지
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    • 제38권4호
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    • pp.382-388
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    • 2010
  • 본 논문에서는 마그네슘합금 재료로 제작된 F-5E/F의 외부 연료탱크 수평 핀의 균열 발생 원인을 분석하고 적절한 대체 재질을 검토하여 탱크의 투하 안전성을 보장하도록 핀의 형상을 재설계하였다. 재료는 재료의 성질과 조달 용이성을 고려하여 강도 및 파괴 인성이 기존재료보다 강하지만 핀 1개당 65%의 무게증가가 예상되는 알루미늄 합금 2024-T351을 선택하였다. 핀의 무게 증가로 인한 연료탱크 무게 중심의 후방 이동을 허용한계 이내로 유지하기 위하여 크기를 20% 축소하고, 두께 7 mm, 곡률 반경 8.5 mm로 설계된 핀에 대하여 정적 및 동적 응력을 해석한 결과 후방 체결부 및 체결 홀의 안전계수가 증가되었음이 확인되었다.