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Comparison of Stress Response in Diallel Crossed Korean Domestic Chicken Breeds (토종 종계를 이용한 이면 교배조합 계통 간 스트레스 반응정도 비교 분석)

  • Cho, Eun Jung;Park, Ji Ae;Choi, Eun Sik;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.43 no.2
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    • pp.77-88
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    • 2016
  • To establish a new synthetic Korean meat chicken breed, we tested $5{\times}5$ diallel cross mating experiment with domestic chicken breeds. Comparing stress responses among diallel crossed chicken breeds, we analyzed telomere length, DNA damage and expressions of heat shock protein genes (HSPs) as the markers of the stress response. The telomere length was measured by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes. The expression levels of HSP-70, $HSP-90{\alpha}$ and $HSP-90{\beta}$ genes were analyzed by quantitative real-time polymerase chain reaction in lymphocytes. The DNA damage rate of lymphocytes was quantified by the comet assay known as the single cell gel electrophoresis. In results, there were significant differences in the values of the stress markers such as telomere length, HSPs and DNA damage rate, and also were significant differences in viabilities and body weights among the $5{\times}5$ diallel crossed chicken breeds. The telomere shortening rate, expression values of HSPs and DNA damage rate were significant low in W and Y crossed chickens compare to the others, but GG pure breed showed the highest values in the 25 crossed chickens. Estimating correlation coefficient, the survival rate positively correlated to telomere length, but negatively correlated to the expression levels of HSP-70, $HSP-90{\alpha}$, $HSP-90{\beta}$ genes and to the value of % DNA in tail as DNA damage rate. The expression levels of HSP-70, $HSP-90{\alpha}$ and $HSP-90{\beta}$ genes of dead chickens had significantly higher than those of survival chickens. According to the results on the stress marker analysis, it would be considered that the crossed breeds had more stress resistant than the pure breeds, and the crossed chickens with a light strain such as W or Y were relatively resistant to stress, but the crossed chickens with a heavy strain such as G, H, F were susceptible to stress.

The Comparative Studies on Hatched Silkworm Dominance Seperation against Sex Seperation to meet Silk Promotion (잠견생산성 개선을 위한 의잠우열분리와 자웅분리의 비교연구)

  • Choe, Byong-Hee
    • Journal of Sericultural and Entomological Science
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    • v.15 no.2
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    • pp.21-28
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    • 1973
  • This report is prepared to promote cocoon natures for the use of silk reeling material. It is easily understandable that there must be disuniformity composed with superior group and inferior group in commercial silkworms. If such different groups be seperated by some method, it would be a great contribution for the cocoon production. For a comparative purpose, silkworm sex seperation carried out because male silkworms produce more silk than female worms. The author has developed a new chemical reagent available for the seperation of superior group and inferior group from commercial silkworms, which he has named it as Better Hybrid Controller (BHC). The obtained comparative results are summarized as followings. 1. Basic investigation of BHC application a) In case BHC applied with hybrid worms and pure line, the former one starts to adapt mulberry leaves earlyer than pure line variety. b) The mulberry adapting interval distribution of pure line worms after BHC application showed U type distribution, but hybrid worms showed L type or Poisson's distribution. c) In case of BHC application with silkworms, the longer period application is, the duller distribution was formed. d) When silkworms are seperated in two groups by use of BHC application, the earlyer mulberry adapted group is seemed as stronger than the other part and the group ratio is 2 : 1. 2. Comparation between sex seperation result and better hydrid control (BHC) seperation result. a) The cocoon shell per cent of male worm group showed betweer result than the female group but only 0.4% difference between sexes. b) The cocoon shell per cent of superior group, seperated by BHC, showed 0.7% more than the inferior group. c) The average cocoon shell per cent of BHC treated cocoons showed much more than the Control group as 1.6∼2.4% increase. Enven the inferior group showed better result than the Control. d) Such unexpected result is considered to be the result that BHC application is activating some thing with silkworm physiology. e) On the ether hand, the result of sexes seperated groups or male worm group did not show desirable conclusion as far as cocoon shell per cent is concerned. f) However, when the male group was reeled as silk, it showed much better silk yield or silk per cent of cocoon than the female group as much as one per cent difference between by sexes. Such result was brought by superior silk yield from cocoon shell as much as 87.4%. g) On the other hand, the male group showed lowest non breaking reelable ratio (63%) among all group comparation. h) When we compare cocoon qualities by sex seperation and BHC seperation against the Control, there is no qualitative change, but BHC group showed quantitative promotion with cocoon bave length as much as about hundred meters. i) In case of calculation for productive income of cocoon production, BHC applied group showed about ten per cent income promotion more than the Control. The sexes seperated group, however, showed rather negative result because the male cocoon produced poor weight per box eggs which could not cover it by the inclose of silk yield of it. j) So, the BHC application with the fetched worm stage brought about big promotion for cocoon production. k) BHC method may be used either for seperation purpose or quantitative promotion with whole silk-worms. 3. Only male silkworms rearing did not show desirable productivity, so there is no reason to work out it in the fetching stage of worm.

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The Effectiveness of CT and MRI Contrast Agent for SUV in 18F-FDG PET/CT Scanning (18F-FDG PET/CT 검사에서 정량분석에 관한 CT와 MRI 조영제의 효과)

  • Cha, Sangyoung;Cho, Yonggwi;Lee, Yongki;Song, Jongnam;Choi, Namgil
    • Journal of the Korean Society of Radiology
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    • v.10 no.4
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    • pp.255-261
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    • 2016
  • In this study, among various factors having influence on SUV, we intended to compare and analyze the change of SUV using CT(4 type) and MRI(3 type) contrast agents which are commercialized now. We used Discovery 690 PET/CT(GE) and NEMA NU2 - 1994 PET phantom as experimental equipment. We have conducted a study as follows; first, we filled distilled water to phantom about two-thirds and injected radioisotope(18F-FDG 37 MBq), contrast agent. Second, we mixed CT contrast agent with distilled water and MRI contrast agent with that water separately. And then, we stirred the fluid and filled distilled water fully not to make air bubble. In emission scan, we had 15minutes scanning time after 40 minutes mixing contrast agent with distilled water. In transmission scan, we used CT scanning and its measurement conditions were tube voltage 120 kVp, tube current 40 mA, rotation time 0.5 sec, slice thickness 3.27 mm, DFOV 30 cm. Analyzing results, we set up some ROIs in 10th, 15th, 20th, 25th, 30th slice and measured SUVmean, SUVmax. Consequently, all images mixed 3 types of MRI contrast agent with distilled water have high SUVmean as compared with pure FDG image but there was no statistical significance. In SUVmax, they have high score and there was statistical significance. And other 4 images mixed 4 types of CT contrast agent with distilled water have significance in both SUVmean and SUVmax. Attenuation correction in PET/CT has been executed through various methods to make high quality image. But we figured out that using CT and MRI contrast agents before PET/CT scanning could make distortion of image and decrease diagnostic value. In that reason, we have to sort out the priority of examination in hospital not to disturb other examination's results. Through this process, we will be able to give superior medical service to our customers.

Effects of an $Al_2$O$_3$Surfasce Protective Layer on the Sensing Properties of $SnO_2$Thin Film Gas Sensors (Al$_2$O$_3$ 표면 보호층이 박막형 $SnO_2$ 가스센서의 감지 특성에 미치는 영향)

  • Seong, Gyeong-Pil;Choe, Dong-Su;Kim, Jin-Hyeok;Mun, Jong-Ha;Myeong, Tae-Ho
    • Korean Journal of Materials Research
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    • v.10 no.11
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    • pp.778-783
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    • 2000
  • Effects of the $Al_2$O$_3$surface protective layer, deposited on the SnO$_2$sensing layer by aerosol flame deposition (AFD) method, on the sensing properties of SnO$_2$thin film ags sensors were investigated.Effects of Pt doping to the $Al_2$O$_3$surface protective layer on the selectivity of CH$_4$ gas were also investigated. 0.3$\mu\textrm{m}$ thick SnO$_2$thin sensing layers on Pt electrodes were prepared by R.F. magnetron sputtering with R.F. power of 50 W, at working pressure of 4mTorr, and at 20$0^{\circ}C$ for 30 min. $Al_2$O$_3$surface protective layers on SnO$_2$layers were prepared by AFD using a diluted aluminum nitrade (Al(NO$_3$).9$H_2O$) solution. The sensitivity of CO gas in the SnO$_2$gas sensor with an $Al_2$O$_3$surface protective layer was significantly decreased. But that of CH$_4$gas remained almost same with pure SnO$_2$gas sensor. This result shows that the selectivity of CH$_4$gas is increased because of the $Al_2$O$_3$surface protective layer. In the case of SnO$_2$gas sensors with Pt-doped $Al_2$O$_3$surface protective layers, low sensing property to CO gas and high sensing property to CH$_4$were observed. This results in the increasing of selectivity of CH$_4$gas selectivity are discussed.

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Forest Community Structure of Mt. Bukhan Area (북한산 지역의 삼림군집구조에 관한 연구)

  • 박인협;이경재;조재창
    • Korean Journal of Environment and Ecology
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    • v.1 no.1
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    • pp.1-23
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    • 1987
  • To investigate the forest structure of Mt. Bukhan. ranging from Seoul to Kyongkido, twenty plots were set up by the vegetation physiognomy and vegetation analysis was carried out. According to the leading dominant tree species in canopy stratum, forest communities were classified into three large groups of natural forest communities, semi-natural forest communities and artificial forest communities, and each of them covered 82.64, 7.03, and 5.71% of Mt. Bukhan area, respectively. Pure or mixed natural forest communities of Pinus densiflora and Quercus mongolica were major forest communities and covered 70.8% of Mt. Bukhan area. The important planted tree species were Robinia pseudoacacia, Pinus rigida, and Alnus birsuta and they were mainly planted at the southern slope and roadside. The degree of human disturbance of vegetation of 8, 7, and 6 area covered 82.64, 0, and 12.74%, respectively. According to forest dimensions, most of forest communities were young aged forests of which mean DBH was 20cm and canopy height below 10m. However, a few mature forest communities of Pinus densiflora or Quercus mongolica were found in the small area. The range of Shannon's species diversity of major natural forest communities, pure or mixed forest communities of Pinus densiflora and Quercus mongolica was 1.085~1.242. According to stand dynamic analysis by DBH class distribution, the present Quercus mongolica communities arid Robinia pseudoacacia communities may last long their present forest structure and most of other communities may be succeeded to Quercus mongolica communities, however, a few communities invaded by Robinia pseudoacacia and Quercus aliena-Quercus acutissima communities may be succeeded to Robinia pseudoacacia communities and Quercus aliena communities, respectively. DCA was the most effective method of this study. DCA ordination were showed that successional trends of tree species seem to be from Pinus densiflora through Quercus serrata. Prunus sargrntii. Sorbus alnifolia to Q. mongolica. Fraxinus mandsburica, F. rhynchophylla in the upper layer and from Zanthoxylum schinifolium, Lespedeza crytobotrya trough Rhus trichocarpa. Rh. verniciflua. Rhododendron mucronulatum. Rh. schlippenbachii to Acer pseudo-sieboldianus. Magnolia sieboldii, Euonymus sieboldianus.

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Technological Synergy Effect of Business Portfolio : Panel Data Analysis on 50 Largest Chaebols in Korea (사업포트폴리오의 기술시너지효과 :50대 재벌의 패널자료분석)

  • 김태유;박경민
    • Proceedings of the Technology Innovation Conference
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    • 1996.12a
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    • pp.265-295
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    • 1996
  • This paper investigates empirically the relationship between various business portfolio properties (particularly technological properties) and chaebol′s performance using data on the 50 largest chaebols in Korea. In addition to the traditional indexes to measure diversification such as entropy index we calculated inter-industry technological similarity using R&D expenditure data by industry and 1990 Input-output Table in Korea, and obtained chaebol-level technological relatedness and internal transaction proportion from chaebols′business profile, inter-industry technological similarity and 1990 input-output table. We applied factor analysis on 13 business portfolio property indexes and showed that they could be grouped into 3 dimensions, diversification scope, inter-business relatedness and degree of vertical integration. In this paper, using 50 largest chaebols′financial data (1989-1994), we analyzed empirically the effect of business portfolio properties on ROS (Return On Sales) which is conventional index for firm performance and on TFP(Total Factor Productivity) growth which is a pure measure of firm performance. To utilize the advantage of panel data, FEM(Fixed Effect Model) and REM(Random Effect Model) were used. The empirical result shows that the entropy index as a measurement of inter-business relatedness is not significant but technological relatedness index is significant. OLS estimates on pooled data were considerably different from FEM or REM estimates on panel data. By introducing interaction effect among the three variables for business portfolio properties, we obtained three findings. First, only VI (Vertical integration) has a significant positive correlation with ROS. Second, when using TFP growth as an dependent variable, both TR(Technological Relatedness) and f[ are significant and positively related to the deepened variable. Third, the interaction term between TR and VI is significant and negatively affects TFP growth, meaning that TR and VI are substitutes. These results suggest strategic directions on restructuring business portfolio. As VI is increased, chaebols will get more profit. A higher level of either TR or W will increase TFP growth rate. but increase in both TR and VI will have a negative effect on TFP growth. To summarize, certain business portfolio properties such as VI and TR can be considered "resources" themselves since they can affect profit rate and productivity growth. VI and TR have a synergy effect of change in profit rate and productivity growth. VI increases ROS and productivity growth, while TR increases productivity growth representing a technological synergy effect.

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Synthesis of Borosilicate Zeotypes by Steam-assisted Conversion Method (수증기 쪼임법에 의한 제올라이트형 보로실리케이트 제조방법)

  • Mansour, R.;Lafjah, M.;Djafri, F.;Bengueddach, A.
    • Journal of the Korean Chemical Society
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    • v.51 no.2
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    • pp.178-185
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    • 2007
  • Intermediate pentasil borosilicate zeolite-like materials have been crystallized by a novel method named steam-assisted conversion, which involves vapor-phase transport of water. Indeed, amorphous powders obtained by drying Na2O.SiO2.B2O3.TBA2O gels of various compositions using different boron sources are transformed into crystalline borosilicate zeolite belonging to pentasil family structure by contact with vapors of water under hydrothermal conditions. Using a variant of this method, a new material which has an intermediate structure of MFI/MEL in the ratio 90:10 was crystallized. The results show that steam and sufficiently high pH in the reacting hydrous solid are necessary for the crystallization to proceed. Characterization of the products shows some specific structural aspects which may have its unique catalytic properties. X-ray diffraction patterns of these microporous crystalline borosilicates are subjected to investigation, then, it is shown that the product structure has good crystallinity and is interpreted in terms of regular stacking of pentasil layers correlated by inversion centers (MFI structure) but interrupted by faults consisting of mirror-related layers (MEL structure). The products are also characterized by nitrogen adsorption at 77 K that shows higher microporous volume (0.160 cc/g) than that of pure MFI phase (0.119 cc/g). The obtained materials revealed high surface area (~600 m2/g). The infrared spectrum reveals the presence of an absorption band at 900.75 cm-1 indicating the incorporation of boron in tetrahedral sites in the silicate matrix of the crystalline phase.

Composition and Neutralization Characteristics of Precipitation at the Anmyeon-do and Gosan GAW Stations from 2008 to 2017 (안면도와 고산 기후변화감시소에서 채취한 강수 성분의 조성 및 중화 특성(2008~2017년))

  • Ko, Hee-Jung;Jeong, Jiyoung;Kim, Eun-Sil;Lee, Sang-Sam;Ryoo, Sang-Boom
    • Atmosphere
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    • v.29 no.4
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    • pp.403-416
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    • 2019
  • Precipitation samples were collected at the GAW Stations in Anmyeon-do and Gosan for 10 years (2008-2017) to analyze pH, electrical conductivity and NH4+, Na+, K+, Mg2+, Ca2+, SO42-, NO3-, Cl-, and F- ions. From the analysis, the correlation between pH and rainfall, the composition of precipitation and comparison with other regions, and the results of neutralization characteristics by seasonal and pH were determined. In the comparison of ion balance and conductivity for the validation of analytical data, the correlation coefficients were within the range of 0.996~0.999, implying good linear relationship. The volume-weighted pH of the Anmyeon-do and Gosan areas were 4.7 and 4.9, respectively. The pH of the rainfall was affected by washout and rainout in both areas. The ionic strength of precipitation at Anmyeondo and Gosan were 0.42 ± 0.63 mM and 0.37 ± 0.75 mM, indicating about 27.6% and 35.3% of the total precipitation as per a pure precipitation criterion (10-4 M), respectively. The composition ratio of ionic species were 44.7% and 57.5% for marine sources (Na+, Mg2+, Cl-), 40.6% and 22.2% for the secondary inorganic components (NH4+, nss-SO42-, NO3-), and 5.6% and 4.0% for the soil source (nss-Ca2+), respectively. The neutralization factor of Anmyeon-do and Gosan were 0.43~0.65 and 0.34~0.48, and the neutralization factors of calcium carbonate were 0.15~0.34 and 0.25~0.30, respectively. Thus, both regions have the highest rate of neutralization caused by ammonia. As pH increased in Anmyeon-do and Gosan, change in calcium carbonate became greater than that in ammonia.

A New Cymbidium Cultivar 'Orange Bowl' with Orange Colored Flower and Medium Sized Plant (오렌지색계 중형 심비디움 'Orange Bowl' 육성)

  • Kim, Mi-Seon;Rhee, Hye-Kyung;Park, Sang-Gun;Jung, Hyang-Young;Choi, Sung-Yul;Lim, Jin-Hee
    • Horticultural Science & Technology
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    • v.29 no.6
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    • pp.651-654
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    • 2011
  • Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

DEVELOPMENT OF SN BASED MULTI COMPONENT SOLDER BALLS WITH CD CORE FOR BGA PACKAGE

  • Sakatani, Shigeaki;Kohara, Yasuhiro;Uenishi, Keisuke;Kobayashi, Kojiro F.;Yamamoto, Masaharu
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.450-455
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    • 2002
  • Cu-cored Sn-Ag solder balls were fabricated by coating pure Sn and Ag on Cu balls. The melting behavior and the solderability of the BGA joint with the Ni/Au coated Cu pad were investigated and were compared with those of the commercial Sn-Ag and Sn-Ag-Cu balls. DSC analyses clarified the melting of Cu-cored solders to start at a rather low temperature, the eutectic temperature of Sn-Ag-Cu. It was ascribed to the diffusion of Cu and Ag into Sn plating during the heating process. After reflow soldering the microstructures of the solder and of the interfacial layer between the solder and the Cu pad were analyzed with SEM and EPMA. By EDX analysis, formation of a eutectic microstructure composing of $\beta$-Sn, Ag$_3$Sn, ad Cu$_{6}$Sn$_{5}$ phases was confirmed in the solder, and the η'-(Au, Co, Cu, Ni)$_{6}$Sn$_{5}$ reaction layer was found to form at the interface between the solder and the Cu pad. By conducting shear tests, it was found that the BGA joint using Cu-cored solder ball could prevent the degradation of joint strength during aging at 423K because of the slower growth me of η'-(Au, Co, Cu, Ni)$_{6}$Sn$_{5}$ reaction layer formed at the solder, pad interface. Furthermore, Cu-cored multi-component Sn-Ag-Bi balls were fabricated by sequentially coating the binary Sn-Ag and Sn-Bi solders on Cu balls. The reflow property of these solder balls was investigated. Melting of these solder balls was clarified to start at the almost same temperature as that of Sn-2Ag-0.75Cu-3Bi solder. A microstructure composing of (Sn), Ag$_3$Sn, Bi and Cu$_{6}$Sn$_{5}$ phases was found to form in the solder ball, and a reaction layer containing primarily η'-(Au, Co, Cu, Ni)$_{6}$Sn$_{5}$ was found at the interface with Ni/Au coated Cu pad after reflow soldering. By conducting shear test, it was found that the BGA joints using this Cu-core solder balls hardly degraded their joint shear strength during aging at 423K due to the slower growth rate of the η'-(Au, Cu, Ni)$_{6}$Sn$_{5}$ reaction layer at the solder/pad interface.he solder/pad interface.

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