• 한국식품영양학회지
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• 제25권1호
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• pp.64-68
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• 2012

It has been suggested that Helicobacter pylori(H. pylori) infections can promote the development and progression of gastric cancer through the modulation of cell cycle regulators such as $p27^{Kip1}$ and Skp2. $p27^{Kip1}$ is a cyclin-dependent kinase (CDK) inhibitor that blocks the G1/S transition necessary for cell cycle progression. Skp2 is a component of the ubiquitin ligase complex called $SCF^{Skp2}$(SKP1-Cullin-F-box), which specifically binds and promotes the degradation of $p27^{Kip1}$. A low level of $p27^{Kip1}$ and a high level of Skp2 have been reported in many types of cancers, including gastric cancer. In addition, a decrease in $p27^{Kip1}$ has been reported in H. pylori-infected specimens. However, data on Skp2 in H. pylori infections are limited. This study examines the changes in the status of Skp2 in H. pylori-infected gastric epithelial AGS cells. For this, we stimulated AGS cells with H. pylori(NCTC 11637) at the ratio of 300:1(bacterium:cell) for 6 hours. The results of an immunoprecipitation analysis, followed by a western blot, indicate that the interaction between Skp2 and 14-3-3 was elevated 3 hours after the H. pylori treatment. In addition, there was an increase in cytoplasmic Skp2 after 3 hours, whereas there was no change in the nuclear level. Since it has been reported that interaction with 14-3-3 and the subsequent cytoplasmic translocation of Skp2 can increase its protein stability, increases in the interaction with 14-3-3 and the cytoplasmic Skp2 after the H. pylori treatment can increase the level of Skp2 in AGS cells. This phenomenon may explain, at least to some extent, the mechanism underlying the relationship between H. pylori infections and gastric carcinogenesis.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.758-763
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• 2012

As a member of a subclass of immunophilins, it is controversial that FKBP38 acts an upstream regulator of mTOR signaling pathway, which control the process of cell-growth, proliferation and differentiation. In order to explore the relationship between FKBP38 and mTOR in the Cashmere goat (Capra hircus) cells, a full-length cDNA was cloned (GenBank accession number JF714970) and expression pattern was analyzed. The cloned FKBP38 gene is 1,248 bp in length, containing an open reading frame (ORF) from nucleotide 13 to 1,248 which encodes 411 amino acids, and 12 nucleotides in front of the initiation codon. The full cDNA sequence shares 98% identity with cattle, 94% with horse and 90% with human. The putative amino acid sequence shows the higher homology which is 98%, 97% and 94%, correspondingly. The bioinformatics analysis showed that FKBP38 contained a FKBP_C domain, two TPR domains and a TM domain. Psite analysis suggested that the ORF encoding protein contained a leucine-zipper pattern and a Prenyl group binding site (CAAX box). Tissue-specific expression analysis was performed by semi-quantitative RT-PCR and showed that the FKBP38 expression was detected in all the tested tissues and the highest level of mRNA accumulation was detected in testis, suggesting that FKBP38 plays an important role in goat cells.

• 치과방사선
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• 제10권1호
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• pp.63-68
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• 1980

The purpose of this article is to re-examine the roentgenographic appearance of alveolar osseous in an effort to determine the value of the orthopantomogram in the diagnosis of periodontal disease. A total of 158 osseous lesions from 13 human dry skulls were studied. 14 Ultra-speed periapical films, 2 bitewing films and Kodak panoramic film are used to obtain radiographs of all defects. The bisecting technic was used, with a target-film distance of. 8 inches and exposure factors of 70 kVp, 10MA and 0.4 sec. at anterior teeth, 0.6 see at posterior teeth in exposure time. For orthopantomogram, Panoura Eight-C was used with a exposure factors of 90kVp, 10MA. and 15sec. exposure time. All films were developed in a light. tight darkroom at 68°F for 4½ minutes. Comparison of orthopantomogram and intraoral films on the view-box was carefully studied in relation to the types of osseous defect visually evident; Proximal intraosseous defects, Interproximal craters, Interproximal hemisepta, Furcal defects on multirooted teeth, and Facial or Lingual one-walled defects. The results obtained were as follows; 1. Proximal osseous defects throughout the dental arches and furcal defects on facial and lingual surfaces of multirooted teeth can be identified with a high degree of accuracy from their orthopantomographic appearances. 2. Lesions on facial or lingual surface of the alveolar arches are rather difficult to locate or recognize on the dental radiographs. 3. In determining whether the proximal and furcal lesions are located facially or lingually, Orthopantomogram is superior to the conventional film Orthopantomogram obtained with standardization of head in proper position revealed the complete visualization of alveolar bone without showing occlusal surface of molars and proximal superimposition of teeth. Thus, on the standardized orthopantomograms, The roentgenographic characteristics of each defect were determined.

• 한국의상디자인학회지
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• 제18권4호
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• pp.141-158
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• 2016

The objective of this study is to examine the type and characteristics of hat, and also to analyze the formative characteristics of hats shown in $Pr{\hat{e}}t-{\grave{a}}$-porter Collection. The literature review focused on the relevant technical books and preceding researches while the case study targeted 1,561 photos of hats shown in the 'Donga TV Collection'. The research range was from the S/S season of 2012 to the F/W season of 2015/16. The results of this study are as follows. 1. A hat is composed of crown and brim. In the results of classifying hats based on it, there were 572 crown-type hats, 533 hat-type, 219 cap-type, 156 irregular-type, and 81 brim-type in the order. 2. For the crown-type hats, they were mainly like cylindrical-type, bell-type, dome-type, and circle-type, using colors like black, beige, brown, red, mustard, and grey, and materials like wool, fur, velvet, straw, and metal while they were decorated with bead or lace. 3. For the hat-type hats, they were mainly like bell-type, ladder-type, and hemisphere-type, using colors like peach, white, gold, brown, and khaki, and materials like silk, cotton, straw, and wool while they were decorated with feather and ribbon. 4. For the cap-type hats, they were mainly like hemisphere-type, using colors like white, black, khaki, mustard, and purple, and materials like fur, leather, and wool while they were decorated with wappen and chain. 5. For the irregular-type hats, they were mainly like sandglass-type, box-type, animal-type, trumpet-type, and bell-type, using colors like purple, orange, pink, yellow, and sky-blue, and materials like metal, feather, mesh, plastic, straw, and linen while they were decorated with ribbon, net, bell, and flower. 6. For the brim-type visor, they were mainly like square-type, using colors like transparent, yellow, and black, and materials like plastic, cotton, leather, and metal.

• 자원환경지질
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• 제35권5호
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• pp.421-428
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• 2002

계면활성제를 사용하여 지하수의 표면장력을 인위적으로 감소하였을 때, 감소된 표면장력이 지하수 폭기시의 폭기 영향권 및 VOC 제거 효율에 미치는 영향을 실험실 규모의 실험을 통하여 규명하였다. 내경 9.5 cm, 길이 100 cm의 유리 컬럼에 계면활성제(sodium dodecyl sulfate, SDS) 및 toluene 100 ppm 포함하는 물로 채운 후 일정한 속도로 공기를 주입하였을 때의 toluene의 제거 속도를 측정하였다. 또한 같은 컬럼에 토양(모래)이 채워진 상태에서 동일한 실험을 반복하였다. 토양이 존재하지 않는 상태에서의 stripping에 의한 toluene의 제거 속도는 표면장력의 감소(계면 활성제 농도의 증가)에 따라 증가하는 것으로 나타났으며, 토양이 존재하는 경우에도 비슷한 결과를 나타내었다. 2차원 유리 상자에 모래와 SBS를 포함하는 물을 채운 후 일정한 공기 유속을 유지하였을 때, 폭기의 영향권(공기의 침투영역)은 물의 표면장력 감소에 따라 현저히 증가하는 것으로 나타났다. 특히 SDS의 critical micelle concentration(CMC)보다 훨씬 낮은 농도에서 폭기 영향권이 최대화하는 것으로 밝혀졌다. 본 연구결과는 폭기에 의한 지하수 오염물질 중, 특히 휘발성 유기오염물질의 제거 공정의 효율을 증대하는데 기여할 것으로 생각된다.

• 한국식생활문화학회지
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• 제14권3호
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• pp.211-224
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• 1999

The purpose of this study was to investigate the understanding of dietary consciousness and define the relationship of housewife attitude of middle class residence in Puchon city. Questionnaires were collected 282 housewives in Puchon city. Data were analysed with SAS software package for F-test and Duncan's multiple range test. The main findings of the research were as follows: The food related perchasing place was supermarket(91%), ordinary market(77%), department store(68%), special store(58%), common purchase(37%), convenience store(22%) and communication marketing(13%). The clothing perchasing place showed in the order of department store(71%), special store(65%), ordinary market(52%), common purchase(23%), supermarket(18%), convenience store(15%) and communication marketing(10%). Dietary cosciousness of housewife on foods, clothing, and housing related items was significantly different, specially 20's housewife was concerned about foods and 30's housewife was housing(p<0.05). The behavior consciousness of housewife was significantly different between age, education level, family type and income(p<0.05). Highly educated housewife showed a tendency to spend money and times for food related fields, and to buy clothes in a department store for breaking stress(p<0.05). However, low education level and extended family type housewife got more conservative consciousness on traditional fermented food making(p<0.05). Most of young housewife answered that the making of bread, cookies, soybean paste soup, children clothes and handicraft led to positive consciousness(p<0.05). Especially high life satisfaction housewife preferred to make a dosirak(lunch box), dinner, children clothes and handicraft(p<0.05).

• 대한의용생체공학회:의공학회지
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• 제16권4호
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• pp.425-430
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• 1995

핵자기 공명 뇌기능 영상에서 상관관계를 이용한 데이터 해석기법이 많이 사용되고 있다. 이 논문에서 새롭게 제안된 CLT 기법은 상관관계(CCT)를 이용한 기법을 변화시켜서, 이 CCT기법의 단점을 보완하고자 하였다. CLT기법은 다음의 두 단계로 이루어진다. 첫째, 잡음을 포함한 CCT기법의 상관계수결과로 부터 잡음은 제거하고 실제 자극반응 픽셀들은 추출한다. 둘째, 이산적인 분포를 가지는 반응 픽셀들을 두 가지의 선별법으로 군집을 이루도록 한다. 이 CLT기법을 이용해서 실제 자극에 반응하는 픽셀들은 서로 모이게 하였고, 잡음에 기인한 오류의 픽셀들은 제거 되어질 수 있었다. 본 논문에서 제안된 CLT기법은 기존의 다른 기법에 비해 여러 잇점이 있고, 특히 잡음에 강한 특성이 있다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.941-944
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• 2008

PVA 섬유, 강섬유를 다량으로 혼입한 고인성 시멘트 복합체가 개발되고 이를 구조물에 활용하고자 하는 연구가 전 세계적으로 수행되고 있다. DFRCC는 경제적 효율성을 고려한다면 현재까지 구조물 전체 부분에 적용하기보다는 특별한 요소나 보수재료서 적용하는 것이 경쟁력이 있을 것으로 사료된다. 저자들은 DFRCC를 FRP-콘크리트 합성 바닥판과 습식 스프레이 보수공법에 적용하는 기술을 개발하고 있다. FRP-콘크리트 합성 바닥판에 적용할 경우에는 현장 적용하여 3개월 경과하더라도 구조성능 또는 내구성능이 저하되는 문제가 발생하지 않았고 매우 양호한 상태를 유지하였다. 그리고 국산 PVA 섬유 사용 DFRCC 보수 모르타르를 20년이 경과된 하수박스암거에 적용한 경우에는 한 결과, 일본산 PVA 섬유를 사용한 경우와 차이가 거의 없었다. 관련 규격인 KS F 4042의 압축 및 휨 강도기준을 모두 만족하는 것으로 나타났다. 이상과 같이 DFRCC를 구조물에 적용할 경우에는 구조물 종류에 따라서 경제적으로 성능 향상에 크게 기여할 수 있을 것으로 판단된다.

• BMB Reports
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• 제51권4호
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• pp.163-164
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• 2018

CONSTANS (CO) induces the expression of FLOWERING LOCUS T (FT) in the photoperiodic pathway, and thereby regulates the seasonal timing of flowering. CO expression is induced and CO protein is stabilized by FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1) in the late afternoon, while CO is degraded by CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) during the night. These regulatory cascades were thought to act independently. In our study, we investigated the relationship between FKF1 and COP1 in the regulation of CO stability in response to ambient light conditions. A genetic analysis revealed that FKF1 acts as a direct upstream negative regulator of COP1, in which cop1 mutation is epistatic to fkf1 mutation in the photoperiodic regulation of flowering. COP1 activity requires the formation of a hetero-tetramer with SUPPRESSOR OF PHYA-105 (SPA1), [$(COP1)_2(SPA1)_2$]. Light-activated FKF1 has an increased binding capacity for COP1, forming a FKF1-COP1 hetero-dimer, and inhibiting COP1 homo-dimerization at its coiled-coil (CC) domain. Mutations in the CC domain result in poor COP1 dimerization and misregulation of photoperiodic floral induction. We propose that FKF1 represses COP1 activity by inhibiting COP1 dimerization in the late afternoon under long-day conditions, resulting in early flowering.

• 말소리와 음성과학
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• 제7권3호
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• pp.139-149
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• 2015

This paper aims to analyze pronunciation variations of loanwords produced by Korean and improve the performance of pronunciation modeling of loanwords in Korean by using syllable-based segmentation and phonological knowledge. The loanword text corpus used for our experiment consists of 14.5k words extracted from the frequently used words in set-top box, music, and point-of-interest (POI) domains. At first, pronunciations of loanwords in Korean are obtained by manual transcriptions, which are used as target pronunciations. The target pronunciations are compared with the standard pronunciation using confusion matrices for analysis of pronunciation variation patterns of loanwords. Based on the confusion matrices, three salient pronunciation variations of loanwords are identified such as tensification of fricative [s] and derounding of rounded vowel [ɥi] and [$w{\varepsilon}$]. In addition, a syllable-based segmentation method considering phonological knowledge is proposed for loanword pronunciation modeling. Performance of the baseline and the proposed method is measured using phone error rate (PER)/word error rate (WER) and F-score at various context spans. Experimental results show that the proposed method outperforms the baseline. We also observe that performance degrades when training and test sets come from different domains, which implies that loanword pronunciations are influenced by data domains. It is noteworthy that pronunciation modeling for loanwords is enhanced by reflecting phonological knowledge. The loanword pronunciation modeling in Korean proposed in this paper can be used for automatic speech recognition of application interface such as navigation systems and set-top boxes and for computer-assisted pronunciation training for Korean learners of English.