• Reproductive and Developmental Biology
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• v.29 no.2
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• pp.69-73
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• 2005

The hematopoietic growth factor erythropoietin (EPO) is required for the maintenance, proliferation, and differentiation of the stem cells that produce erythrocytes. To analyse the biological activity of the recombinant human EPO (rec-hEPO), we have cloned the EPO cDNA and genomic DNA and produced rec-hEPO in the CHO cell lines. The growth and differentiation of EPO-dependent human leukemic cell line (F36E) were used to measure cytokine dependency and in vitro bioactivity of rec-hEPO. MIT assay values were increased by survival of F36E cells at 24h or 72h. The hematocrit and RBC values were increased by subcutaneous injection of 20 IU (in mice) and 100IU(in rats) rec-hEPO. Hematocrit values remarkably increased at $13.2\%$ (in mice) and $12.2\%$ (in rats). The pharmacokinetic behavior with injection of 6 IU of rec-hEPO remained detectable after 24 h in all mice tested. The highest peat appeared at 2h after injection. The long half-life of rec-hEPO is likely to confer clinical advantages by allowing less frequent dosing in patients treated for anemia. These data demonstratethat ree-hEPO produced in this study has a potent activity in vivo and in vitro. The results also suggest that biological activity of ree-hEPO could be remarkably enhanced by genetic engineering that affects the potential activity, including mutants with added oligosaccharide chain and designed to produce EPO-EPO fusion protein.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.5
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• pp.476-484
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• 2020

Purpose: This study determined the intention of obtaining long-term work by graduate nursing students. Methods: The subjects were 116 students in the fourth year of the nursing department located in G city of G province and G city of J province. A structured questionnaire was used from November 1, 2018 to November 27, 2018 for data collection. The SPSS/WIN 21.0 program was used for the collected data and the data was analyzed by t-tests, one-way ANOVA, Pearson's correlation coefficients, and stepwise multiple regression analysis. Result: The major factors influencing the intention to work were satisfaction with their academic major (β=.54, p<.001), and the career after employment in a hospital) (β=.28, p<.001). The explanatory power was 46.5% (F=51.00, p<.001). Conclusion: To increase the satisfaction of studying nursing, it is necessary to apply various educational programs to positively recognize the nursing profession and a nurse's image and increase optimism and social efficacy. There is a need to develop a tool to measure the intention of obtaining long-term nursing jobs for nursing students in hospitals. This study suggested a way to increase the intention of obtaining long-term work by improving the degree of satisfaction with nursing profession.

• Journal of Korean Ophthalmic Optics Society
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• v.15 no.4
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• pp.355-360
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• 2010

Purpose: Analysis performance for spectacle lens which sales in domestic market and optimization design a spectacle lens which is corrected aberration. Methods: Measured center thickness, radius and aspherical surface coefficient for spherical and aspherical lenses which were ${\pm}$5.00D. Refractive index for every lens was 1.6 and they came from 4 different companies. I used 3 types of equipment to measure lenses. ID-F150 (Mitutoyo) : Center Thickness, FOCOVISION (SR-2, Automation Robotics) : Radius, PGI 1240S (Taylor Hobson) : Aspherical surface coefficient. Designed a lens which had 27 mm of distance from lens rear surface to center of eye, 4 mm of pupil diameter and small aberration on center vision $30^{\circ}C$. To shorten axial distance compared with the measured lens rise merits for cosmetic. Lens Design tool was CODE V (Optical Research Associates). Results: -5.00D aspherical lens had somewhat high astigmatism and distortion compared with the spherical lens. But it had a merit for cosmetic because of short axial height and decrease edge thickness. Improved a performance of distortion and ascertain merits for cosmetic due to short axial height and decrease edge thickness same as (-) lens in case of +5.00 aspherical lens. Though an optimization process front surface aspherical lens had a good performance for astigmatism and distortion and the merit for beauty compared with measured spherical lens. Conclusions: Design trend for domestic aspherical lens is decrease axial height and thickness to increase a merit for cosmetic not but increase performance of aberration. From design theory for optimization design front surface aspherical spectacle lens which has improved performance of aberration and merit for cosmetic at the same time compared with the measured lens. Expect an improved performance from design back aspherical lens compared with front aspherical lens.

• Journal of the Korea Society for Simulation
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• v.15 no.3
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• pp.23-30
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• 2006

A recommender system for E-commerce site receives information from customers about which products they are interested in, and recommends products that are likely to fit their needs. In this paper, we investigate several methods for large-scale product purchase data for the purpose of producing useful recommendations to customers. We apply the traditional data mining techniques of cluster analysis and collaborative filtering(CF), and CF with reduction of product-dimensionality by use of latent semantic indexing(LSI). If reduced product-dimensionality obtained from LSI shows a similar latent trend of customers for buying products to that based on original customer-product purchase data, we expect less computational effort for obtaining the nearest-neighbor for target customer may improve the efficiency of recommendation performance. From simulation experiments on synthetic customer-product purchase data, CF-based method with reduction of product-dimensionality presents a better performance than the traditional CF methods with respect to the recall, precision and F1 measure. In general, the recommendation quality increases as the size of the neighborhood increases. However, our simulation results shows that, after a certain point, the improvement gain diminish. Also we find, as a number of products of recommendation increases, the precision becomes worse, but the improvement gain of recall is relatively small after a certain point. We consider these informations may be useful in applying recommender system.

• The Journal of Korean Academic Society of Nursing Education
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• v.9 no.2
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• pp.264-275
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• 2003

The purpose of this study were to develop a smoking preventive education program for elementary students and evaluate it's effectiveness. This study was a quasi experimental study under the nonequivalent control group with pretest-posttest design. The subjects of this study were 62 who are attending elementary school(31 for each group), 2 different district elementary school. The subjects were matched by grade, similar in anti-smoking educational background of smoking, as well as their residence and income level of their families. The instruments used in this study was 18 criterion referenced test items modeled by Dick & Carey that were developed by researchers for evaluating the subjects' knowledge and attitude about smoking. A pretest was administered a week before treatment The program given to the experimental group is composed of the texts explaining the poisonous substances in tobacco, social and cultural harmfulness of smoking to the body and psychology, indirect smoking, smoking of pregnant women, motives of smoking, refusal skills of smoking; and for the subjects' understanding and the better results of study - pictures, role play, discussion, text through computer based multi-media, puzzle searching for hidden pictures, cross-word puzzle, and finally compensation. The data were collected for 50 days form mid- September to the end of October in the year of 2000, composed of formative evaluation, pre-test and summative evaluation via 2 sessions. Accordingly, the collected data were analysed by t-test, paired t-test, repeated measure ANOVA by the SAS program. This research summarize the findings as follows; 1. There was a significant difference in knowledge between the experimental group(after 1 wks t=10.4680, p=.0001; after 4 wks t= 9.310, p=.0001) and control group(after 1 wks t=0.0420, p= .9669; after 4 wks t= -0.378 p=.7079) in between the results of 1 and 4 week after education in summative evaluation (F=27.45, P=.0001). 2. There was non statistical significant difference in attitude between the experimental group (after 1 wks t=1.2292, p=0.2286 ; after 4 wks t=1.330, p=0.1935) and control group (after 1 wks t=0.1819, p=0.8569 ; after 4 wks t=0.2970, p=0.7685) in between the results of 1 and 4 week after education in summative evaluation(F=0.71, P=0.494). To sum up, the statistics of conclusive analysis evaluative for the children under school age of the 'knowledge acquisition' about smoking harmfulness. On the other hand, as there was already sound attitude about smoking, the evaluation of attitude was non significant difference between control group and experimental group, just there was partially significant difference.

• Research in Community and Public Health Nursing
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• v.8 no.2
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• pp.262-276
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• 1997

This study evaluates the relationship between health belief. self-efficacy and exercise and diet compliance in coronary heart disease patients. The study subjects were 96 CHD patients who visited the outpatient clinic at 3 general hospitals in Taegu city from April 3, 1997 to May 3, 1997. Data was collected by the researcher and two registered nurses who work at a cardiac outpatient clinic. Face-to-face interview was conducted. Moon's health belief scale(l990) and Hicky and others' Cardiac Diet Self-Efficacy Instrument (CDSEI, 1992), Cardiac Exercise Self-Efficacy Instrument (CESEI, 1992) were used. The instrument developed by the researcher based on the reference review was used to measure exercise and diet compliance. The data were analyzed by using descriptive statistics, Pearson correlation coefficient, t-test, ANOVA, Tukey verification and Stepwise multiple regression with the SAS program. The results of this study were as follows; 1. The degree of health belief(score range: 1-4) perceived benefit: 3.06 barrier: 2.04 severity: 2.93 2. The degree of self-efficacy(score range: 1-5) exercise self-efficacy: 2.91 diet self-efficacy: 3.32 3. The degree of compliance (score range: 1-4) exercise compliance: 2.34 diet compliance: 2.95 4. The exercise compliance had a positive correlation with perceived benefit(r=0.5327, p=0. 0001), severity(r=0.2780, p=0.0061), exercise self-efficacy(r=0.6675, p=0.0001), and a negative correlation with barrier{r= -0.4236, p=0.0001). The diet compliance had a positive correlation with perceived benefit (r=0.6439, p=0.0001), severity(r=0.4244, p=0.0001), diet self-efficacy(r=0.6629, p=0.0001), and a negative correlation with barrier{r= -0.5098, p=0.0001). 5. According to pt's education level, (F=3.02, p=0.0336), received massage from mass media on exercise and diet(t=3.81, p=0.0002), presence of cardiac patients in the family members or friends(t=2.00, p=0.0478), created significant differences in exercise compliance. According to occuption(F=3.03, p=0.0215), hospitalized experience(t=4.59, p=0.0000), presence of chest pain(t=3.63, p=0.0005), there was also a significant difference in diet compliance. 6. The combination of exercise self-efficacy, perceived benefit and pt's education level explained 50.18% of the variance in exercise compliance. The combination of diet self-efficacy, perceived benefit and barrier explained 56.76% of the variance in diet compliance. On the basis of the above findings, the follow ing recommendations are suggested: 1. To promote the exercise. diet compliance for CHD patients, a well organized health teaching and nursing intervention program should be developed. 2. More research is needed to investigate other variables affecting exercise and diet compliance of CHD patients. 3. To promote self-efficacy and a positive health belief in CHD patients, a well organized and an approachable nursing intervention program should be developed. 4. Factors other than diet. exercise compliance should be evaluated to discover the impact on CHD patients.

• Journal of the Korea Concrete Institute
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• v.14 no.6
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• pp.973-981
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• 2002

Recently, the use of high strength concrete (HSC) has increased dramatically md however, few studies have been conducted on the early-age properties of HSC such as setting. The penetration resistance test (specified by KS F 2436) that is the standard test method for determining initial and final setting times of concrete, may not be appropriate for HSC because of the high viscosity of the mortar mixture. To address this issue, an ultrasonic pulse velocity (UPV) monitoring system was used to investigate the setting behavior of mortar and concrete. The experimental study was carried out to measure the UPV's of mortars and concretes having various water/binder ratios (W/B) and various fly ash replacement levels, during the first 24 hours of testing. Test results showed that the UPV in concrete was developed faster than that of mortar with the same W/B, and that of ordinary concrete was greater than that of fly ash concrete. Typical values of UPV were suggested that correspond to the initial and final setting times, based on following criteria: (1) the penetration resistance method; (2) the instant when the UPV begins to develop; and (3) the instant when the UPV development rate is maximum. The method and UPV monitoring device used in this study is promising for the setting assessment of concrete, particularly for HSC.

• Journal of the Korean Geotechnical Society
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• v.19 no.6
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• pp.15-22
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• 2003

In the conventional load transfer analysis for a steel pipe drilled shaft, it was assumed that the concrete's strain is the same as the measured steel's strain and the elastic modulus of the steel and the concrete calculated by the formular as prescribed by specification is used in the calculation of pile axial load. But, the pile axial load calculation by conventional method differed to some extent from the actual pile load. So, the behavior of a steel pipe drilled shaft could not be analyzed exactly. Thus, the necessity to measure the strain for each pile component was proposed. In this study, a new approach for load transfer measurement of large diameter drilled shafts was suggested ; the strain of each pile component(i. e., steel and concrete) was measured by DRS(Deformable Rod Sensor), the elastic modulus was determined by the uniaxial compression test for concrete specimens made at test site and a value of elastic modulus was evaluated as average tangential modulus corresponding to the stress level of the (0.2-0.6)$f_{ck}$. Field application was confirmed by the results of load transfer measurement tests for 3 drilled shafts. The errors for calculated pile head load were -11 ∼16% and 3.4% separately.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.11
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• pp.1999-2010
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• 1992

The fixed slab analogy device which can measure stress intensity factors(S.I.F) experimentally by slab analogy theory is developed in this paper. The margin of errors resulted from the new testing apparatus are between 0.02% and 8.25%. Therefore, it is assured that this one can be effectively used for the more accurate measurement of S.I.F.( $k_{I}$, $k_{I I}$) than conventional apparatus. The pitch of master grating used in this experiment is 0.1mm It is known that the ratio of the distance from crack tip to the crack length on obtaining the accurate stress intensity factor is between 0.4 and 0.7. The optimum curvature radius of slab is about 125mm. The thickness of slab(plate) used in the fixed slab analogy device is 0.05mm(P.V.C. ; E = 64 MN/ $m^{2}$, .nu.=0.38), which is proved to be suitable for the test. The optimum material for the frame(slab`s external boundary) is a alloy tool steel(SKS 5) plate and its thickness is 1mm. In this research, the rigid cracks are directly bonded to the slab surface by cyanoacrylate adhesive for the easiness of slab making and conformity to the practical crack figure. The material of rigid crack is thin steel plate. It is expected that the developed method can be used effectively for the analysis of $k_{I}$ and $k_{I I}$ of arbitrary shaped or distributed cracks.cks.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.5
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• pp.691-699
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• 2013

Three experiments were conducted to evaluate the metabolizable energy (ME) value, standardized ileal digestibility (SID) of amino acids (AA) of soybean meal (SBM), soy protein concentrate (SPC) and fermented soybean meal (FSBM), and the application of these products in early-weaned piglets. In Exp. 1, four barrows with initial body weight (BW) of $14.2{\pm}1.4$ kg were used in a $4{\times}4$ Latin square design. The diet 1 contained corn as the only energy source. The other three diets replaced 25% of corn in diet 1 with one of the three soybean products, and the digestable energy (DE) and ME contents were determined by difference. In Exp. 2, four barrows (initial BW of $18.2{\pm}1.5$ kg) were fitted with ileal T-cannulas and allotted to a $4{\times}4$ Latin square design. Three cornstarch-based diets were formulated using each of the soybean products as the sole source of AA. A nitrogen-free diet was also formulated to measure endogenous losses of AA. In Exp. 3, ninety six piglets (initial BW of $5.6{\pm}0.9$ kg) weaned at $21{\pm}2$ d were blocked by weight and assigned to one of three treatments for a 21-d growth performance study. The control diet was based on corn and SBM, the two treatments' diets contained either 10% SPC or FSBM and were formulated to same SID lysine to ME ratio of 3.6 g/Mcal. The results showed that the ME content of SPC was greater than SBM (p<0.05). The SID of most AA in SPC was greater than the SID of AA in SBM (p<0.05). For the essential AA, the SID of histidine, isoleucine, leucine, lysine and threonine in FSBM were greater than in SBM (p<0.05). Even though they were fed same SID lysine to ME ratio of 3.6 g/Mcal diets, pigs fed SPC and FSBM diets had greater weight gain, G:F (p<0.05) and better fecal score (p<0.05) than pigs fed SBM diet. In conclusion, SPC showed a higher ME content and SID of AA than the SBM. SID of some essential AA in FSBM was higher than SBM and was similar with SPC. But the lower antigenic proteins and anti-nutritional factors content in SPC and FSBM may be the main factors affecting the performance of early-weaned piglets rather than the increased ME content and SID of AA.