• Research in Plant Disease
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• v.25 no.4
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• pp.157-163
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• 2019

This study aimed to assess the incidence and distribution of toxigenic fungi in Korean oat. Toxigenic fungi were isolated from oat samples collected from 12 oat fields from heading to harvest in 2017 and 2018. A total of 745 fungal colonies were isolated based on morphology and identified using marker genes. About 92% of the fungal isolates were Fusarium spp. and others were Penicillium (5.9%) and Aspergillus (2.1%). Fusarium isolates comprised mostly of F. asiaticum (83.1%), followed by F. incarnatum (5.4%), F. proliferatum (3.5%), F. fujikuroi (2.8%), F. tricinctum species complex (FTSC) 11 (1.5%) and F. graminearum (1.0%). About 97% of F. asiaticum was nivalenol type, and 3-acetyl deoxynivalenol (3.2%) and 15-acetyl deoxynivalenol (0.4%) types also were found. Pathogenicity test of the selected Fusarium isolates revealed that F. asiaticum isolates have a wide range of virulence depending on the tested plants. F. graminearum and FTSC 11 isolates from blighted spikelets were the most virulent in naked oat. All Fusarium isolates (n=18) except one (FTSC 11) produced nivalenol (0.2-7.6 ㎍/g), deoxynivalenol (0.03-6.1 ㎍/g), and zearalenone (0.1-27.0 ㎍/g) on rice medium. This study is first report that F. asiaticum causes Fusarium head blight disease of oat in Korea. These findings demonstrate the dominance of F. asiaticum in oat agroecosystems as in rice, wheat and barley in Korea.

• The korean journal of orthodontics
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• v.29 no.5 s.76
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• pp.511-519
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• 1999

The shape of orthodontic retraction spring was varied and force system of each case was obtained using numerical analysis and verified with spring tester. The factors for obtaining biomechanically efficient spring under anatomic limitation were suggested as follows. 1. M/F ratio increases and L/D rate decreases as loop height increases. 2. M/F ratio increases and L/D rate decreases as incorporating more wire above minimum bending moment area. 3. M/F ratio decreases and L/D rate decrease as incorporating more wire below minimum bending moment area. 4. M/F ratio can not be greater than spring height no matter how much wire is incorporated at the apex of the loop. 5. Additional moment is necessary to obtain enough M/F ratio for translation under anatomical limitation. 6. Additional moment should be incorporated at every pah of the spring because M/F ratio and L/D rate decreases as horizontal spring length increases. 7. Material, cross section, and shape of the spring influence L/D rate, whereas M/F ratio is influenced by the shape of the spring independent from material and cross section.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.2
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• pp.205-213
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• 2011

The Folin-Ciocalteu (F-C) reagent has been extensively used for quantifying total phenolic contents in many different types of food materials. Since several different procedures of the assay methods using the F-C reagent have been applied, we investigated changes in reactivity of various phenolic compounds with the F-C reagent under three different assay conditions and factors affecting reactivity. Among 10 standard compounds tested, compounds with high hydroxyl density (number of -OH/molecular weight) showed a largely different response according to addition sequence of the F-C reagent or $Na_2CO_3$. Preincubation in $Na_2CO_3$ significantly reduced the reactivity of the phenolic compounds bearing galloyl moiety (e.g. gallic acid, tannic acid, and epigallocatechin-3-gallate) with the F-C reagent, while monophenol compounds including ferulic acid and sinapinic acid were more stable as compared to diphenols. There was little change in response to the F-C reagent of all phenolic compounds incubated in acidic pH; their reactivity except ferulic acid was reduced significantly when incubated in neutral or alkaline pH. Changes in reactivity of gallic acid incubated in $Na_2CO_3$ or neutral/alkaline pH conditions were the most prominent. $H_2O_2$ generated from phenolic compounds did not affect the reaction with the F-C reagents. The present results suggest that reactivity of different phenolic compounds with F-C reagent was affected considerably by different procedures of the assay, and the total phenolic contents could be fluctuated according to standard compounds and assay scheme.

• Economic and Environmental Geology
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• v.42 no.3
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• pp.217-234
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• 2009

This study characterized groundwater quality and the influence of agrochemicals in a part of Ilgwang-Myeon agricultural area in Gijang-Gun, Busan Metropolitan City, using factor analyses. From the 1st, 2nd, and 3rd analyses of groundwater samples, the mean concentrations of $Ca^{2+}$, $Na^+$, $Mg^{2+}$, $K^+$, $Zn^{2+}$, $Cu^{2+}$, $Fe^{2+}$, $Al^{3+}$, $NO_3\;^-$, $Cl^-$, $SO_4\;^{2-}$, $F^-$, and $SiO_2$ were higher in the 2nd analysis than the other analyses. Pesticide carbofuran and herbicide alachlor were detected at the wells more than a half of all the wells in the 2nd analysis but not in the 4th analysis. This fact may be explained by that a higher precipitation induced higher infiltration rate of contaminants into groundwater during the 2nd survey. According to R- and Q-mode factor analyses, and chemical composition, inorganic constituents excepting $SiO_2$, $HC0_3\;^-$-, and $F^-$ may be influenced by anthropogenic sources (manures, synthetic fertilizers, and domestic wastes), seawater as well as water-mineral interaction. A typical indicator of groundwater contamination, nitrate, exceeds around 4-5 times over the Korean standard for drinking water. Additionally, the influence of seawater diminishes from the seaside to inland.

• Analytical Science and Technology
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• v.8 no.4
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• pp.1069-1074
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• 1995

The application of scanning electrochemical microscopy to the imaging of surfaces in water and air and to the study of the electrochemistry of single molecules is discussed.

• Reproductive and Developmental Biology
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• v.38 no.1
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• pp.53-62
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• 2014

The objective of this study was to investigate the relationship between fertility and protein pattern change using in vitro fertilization, analysis of sperm characteristics and two-dimensional gel electrophoresis in different pig types. In results, the viability and mitochondria integrity of sperm were higher significantly (p<0.05) but the portions of acrosome reaction was lower significantly (p<0.05) in Duroc and $F_1$ (potbellied ${\times}$ PWG miniature pig) than PWG miniature. On in vitro fertilization to investigate fertility, the fertility of $F_1$ semen war higher significantly (p<0.05) than in Duroc and PWG miniature pig. On the other hand, protein patterns showed similar function among the different boar semen. Especially, the heat shock 70 kDa 1-like and G patch domain-containing protein 4 were significantly (p<0.05) higher expressed in $F_1$ than in Duroc and PWG miniature pig. The proteins associated with mitochondria in Duroc were significantly (p<0.05) higher expressed than in $F_1$ and PWG miniature pig. The developmental rates to blastocyst stage of oocytes fertilized with sperm of $F_1$ pig were significantly (p<0.05) higher than in PWG miniature pig. However, phosphoglycerate kinase 2 and zinc finger protein 431 were significantly (p<0.05) higher expressed in PWG miniature pig than in $F_1$ and Duroc pigs. In conclusion, the results of the present study indicate that different proteins were expressed in different pig types, and were associated with a sperm functions and embryo development.

• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.87-89
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• 2007

선행 연구개발한 집단감시 디지털 시스템(Group Digital Surveillance System for Fishery Safety and Security, GDSS- F2S)은 대단위 양식장에 침입하는 도적을 방어하기 위하여 레이더 추적정보와 적아식별 정보를 제공하는 시스템이다. 그러나 GDSS-F2S에서 제공하는 두 가지 정보만으로는 도적행위를 입증할 수 있는 법적 증거자료로 미흡하다. 본 논문에서는 이러한 문제점 해결방안의 하나로 CCD 카메라를 이용한 영상획득 장치를 개발하여 GDSS-F2S에 부가한 연구내용을 기술한다. 영상획득 장치 개발에 앞서, 실험지역의 지리적인 특성을 고려한 도적침입 예상경로 분석과 도적행위 시나리오를 기반으로 유효한 대응수단을 검토한 결과, 영상획득 장치가 유효한 대응수단임을 확인하였다. 영상획득 장치는 저가이변서 성능이 우수한 0.0001 룩스의 초저조도 CCD 카메라와 부가장치를 이용하여 개발하였다. 6개월 이상의 장기 현장실험을 통하여 본 연구에서 개발한 시스템을 평가한 결과, 주간은 물론 1 미터 앞도 식별할 수 없는 야간에도 차량의 행동과 번호판 및 사람의 행동과 인상착의 등의 영상정보를 확보할 수 있었다.

• Journal of the Korean Solar Energy Society
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• v.33 no.5
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• pp.41-50
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• 2013

Economic feasibility study using weighted average variable Jeju System Marginal Price, SMP, was conducted for Gasiri wind farm of Jeju Island. To predict the variable Jeju SMP, generator share ratio for SMP was calculated from the real time wind power production and the power demand data for years. Also, sensitivity analysis on Net Present Value, NPV, and Benefit/Cost Ratio, B/C ratio, were performed to clarify which factors are more important in assessing economic feasibility. The result shows that the Gasiri wind farm has a minimum of 110 billion won and a maximum of 132 billion won difference between fixed and variable SMP. Also, Capacity Factor, C.F., had the highest sensitivity for NPV, followed by SMP. Accordingly, when economic analysis for a potential wind farm site is carried out, the variable SMP as well as C.F. should be considered for more accurate assessment of the wind farm.

• KSBB Journal
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• v.22 no.5
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• pp.345-350
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• 2007

Phosphosugars are found in all living organisms and are commercially valuable compounds with possible applications in the development of a wide range of specialty chemicals and medicines. In carbohydrate metabolism, fructose 6-phosphate (F6P) is an essential intermediate formed by phosphorylation of 6' position of fructose in glycolysis, gluconeogenesis, pentose phosphate pathway and Calvin cycle. In glycolysis, F6P lies within the glycolysis metabolic pathway and is produced by isomerisation of glucose 6-phosphate. For large-scale production, F6P could be produced from starch using many enzymes such as pullulanase, starch phosphorylase, isomerase and mutase. In enzymatic reactions carried out at high temperatures, the solubility of starch is increased and microbial contamination is minimized. Thus, thermophile-derived enzymes are preferred over mesophile-derived enzymes for industrial applications using starch. Recently, we reported the production of glucose 1-phosphate (G1P) from starch by Thermus caldophilus GK24 enzymes. Here we report the production of F6P from starch through three steps; from starch to glucose 1-phosphate (glucan phosphorylase, GP), then glucose 6-phosphate (phosphoglucomutase, GM) and then F6P (phosphoglucoisomerase, GI). Using 200 L of 1.2% soluble starch solution in potassium phosphate buffer, 1,253 g of G1P were produced. Then, 30% yields of F6P were attained at the optimum reaction conditions of GM : G1 (1 : 2.3), 63.5$^{\circ}C$, and pH 6.85. The optimum conditions were found by response surface methodology and the theoretical values were confirmed by the experiments. The optimum starch concentrations were 20 g/L under the given conditions.

• Journal of the Korean Electrochemical Society
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• v.3 no.4
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• pp.204-210
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• 2000

The control of dissolved aluminum concentration in the hot dip zinc galvanizing bath is greatly important in producing galvannealed steel sheets. The purpose of present study is to provide basic data for measurement of the aluminum concentration in site in hot dip zinc bath at the temperature of $460^{\circ}C\~500^{\circ}C$ using $CaF_2$ solid electrolyte sensor with three kinds of reference electrode. Good workability and stability of the sensor were confirmed with the $Bi+BiF_3$ reference electrode from the emf measurement. In order to measure the aluminum concentration in Zn-Al bath, the galvanic cell of fluorine ion was constructed with $CaF_2$ solid electrolyte as follows; $$(-)W|Zn-Al,\;AlF_3|CaF_2|Bi,BiF_3|W(+)$$. The emf measurement was made at the temperature of $460\pm10^{\circ}C$ in the Zn-Al bath. The following correlationship between aluminum concentration and emf was obtained by the least square regression analysis; $$E/mV=56.795log[\%Al]+1881.7\;R=0.9704$$,$$0.026wt\%{\leq}[\%Al]{\leq}0.984wt\%$$