• Research in Plant Disease
• /
• v.22 no.3
• /
• pp.152-157
• /
• 2016

Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and effective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a $90^{\circ}$ angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension ($1.0{\times}10^7conidia/ml$) on soil. The inoculated plants are cultivated in a growth room at $25^{\circ}C$ for about 4 weeks with 12-hour light a day. The proposed screening method enables to effectively select resistant from mass radish plants cultivars to Fusarium wilt.

• Horticultural Science & Technology
• /
• v.31 no.1
• /
• pp.110-116
• /
• 2013

This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than $1{\times}10^7\;conidia{\cdot}mL^{-1}$) of FOL was poured directly on the roots. The infected plants were cultivated in a growth room at $25-30^{\circ}C$ for 4 weeks with 12-hours light a day.

• Horticultural Science & Technology
• /
• v.31 no.5
• /
• pp.626-632
• /
• 2013

In the course of a developing screening method for resistant radish to Fusarium oxysporum f. sp. raphani, we found that the fungus produces phytotoxic compound against Raphanus sativus. The culture filtrate of F. oxysporum f. sp. raphani KR1 represented the strongest phytotoxicity when the fungus was incubated in the malt extract broth with 150 rpm at $25^{\circ}C$ for 14 days. Under bioassay-guided purification, we isolated a substance from liquid culture of F. oxysporum f. sp. raphani KR1, with phytotoxic effect against R. sativus. The compound was identified as fusaric acid by mass and nuclear magnetic resonance spectral analyses. Phytotoxicity of the compound against cruciferous vegetable crops, including radish, cabbage, and broccoli, was investigated. Fusaric acid represented phytotoxicity on radish seedlings by concentration dependant manner. And the phytotoxin demonstrated strong phytotoxicity on the resistant cultivars as well as susceptible cultivars of radish to F. oxysporum f. sp. raphani. In addition, fusaric acid isolated from the fungus also showed a potent phytotoxic efficacy against non-host Brassicaceae crops of the fungus such as cabbage and broccoli. The results demonstrate that fusaric acid produced by F. oxysporum f. sp. raphani is non-host-specific toxin and for screening of resistant radish to the fungal pathogen, spore suspension of the fungus without the phytotoxin has to be used.

• Horticultural Science & Technology
• /
• v.33 no.1
• /
• pp.70-82
• /
• 2015

This study was conducted to establish an efficient screening system to identify melon resistant to Fusarium oxysporum f. sp. melonis. F. oyxsporum f. sp. melonis GR was isolated from infected melon plants collected at Goryeong and identified as F. oxysporum f. sp. melonis based on morphological characteristics, molecular analyses, and host-specificity tests on cucurbits including melon, oriental melon, cucumber, and watermelon. In addition, the GR isolate was determined as race 1 based on resistance responses of melon differentials to the fungus. To select optimized medium for mass production of inoculum of F. oxysporum f. sp. melonis GR, six media were tested. The fungus produced the most spores (microconidia) in V8-juice broth. Resistance degrees to the GR isolate of 22 commercial melon cultivars and 6 rootstocks for melon plants were investigated. All tested rootstocks showed no symptoms of Fusarium wilt. Among the tested melon cultivars, only three cultivars were susceptible and the other cultivars displayed moderate to high resistance to the GR isolate. For further study, six melon cultivars (Redqueen, Summercool, Superseji, Asiapapaya, Eolukpapaya, and Asiahwanggeum) showing different degrees of resistance to the fungus were selected. The development of Fusarium wilt on the cultivars was tested according to several conditions such as plant growth stage, root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease. On the basis of the test results, we suggest that an efficient screening method for melon plants resistant to F. oxysporum f. sp. melonis is to remove soil from roots of seven-day-old melon seedlings, to dip the seedlings without cutting in s pore s uspension of $3{\times}10^5conidia/mL$ for 30 min, to transplant the inoculated seedlings to plastic pots with horticulture nursery media, and then to cultivate the plants in a growth room at 25 to $28^{\circ}C$ for about 3 weeks with 12-hour light per day.

• The Korean Journal of Applied Statistics
• /
• v.12 no.2
• /
• pp.553-561
• /
• 1999

이변량 정규분포의 상관계수 $\rho$에 대한 검정 및 신뢰구간을 구하는 모수적 방법으로서 Fisher의 z 변환과 해당하는 점근적 분포가 널리 쓰이고 있다. 본 연구에서는 이에 대한 대안으로서 직교변환과 F 분포를 활용하는 방법을 제시한다. 후자의 방법이 전자와 비교하여 사실상 대등하면서도 설명은 오히려 쉬우므로 통계학 교육에 더 적합하다고 생각한다. 또한, 시험적으로, $H_0$:$\rho$=$\rho_0$에 대한 모수적 임의화 검정법을 제안한다.

• Proceedings of the Korea Contents Association Conference
• /
• 2011.05a
• /
• pp.209-210
• /
• 2011

본 연구는 학교보안관 제도에 대한 학부모 인식을 조사하는데 목적을 두고 있다. 이를 위하여 이 연구에서는 서울시 4개구를 무작위로 선정하여 107명을 추출하여 분석하였다. 설문지의 타당도는 전문가회의와 요인분석, 신뢰도는 신뢰도 분석을 통하여 검증하였다. 통계처리는 카이스퀘어 검증과 t검정, F검정 등이 실시되었다. 결론은 다음과 같다. 첫째, 개인적 특성에 따라 학교보안관 제도의 필요성은 차이가 있다. 둘째, 개인적 특성에 따라 학교보안관의 학교생활 도움정도는 차이가 있다. 셋째, 개인적 특성에 따라 학교보안관 만족은 차이가 있다.

• Research in Plant Disease
• /
• v.17 no.1
• /
• pp.13-18
• /
• 2011

This study was conducted to establish the efficient screening method for resistant cabbage to Fusarium wilt caused by Fusarium oxysporum f. sp. conglutinans. The resistance degrees of nine commercial cabbage cultivars to the disease were evaluated. Among them, five cultivars (YR-honam, Ogane, Greenhot, Redmat, and Ccoccoma) showing different resistance to the fungus were selected. Then development of Fusarium wilt of the cultivars according to several conditions including root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease was investigated. Highly resistant cultivars such as 'YR-honam' and 'Ogane' hardly showed change of resistance to the disease by root wounding, dipping period, and inoculum concentration, while disease severity of Fusarium wilt on the cultivars was changed with incubation temperatures ($20^{\circ}C$, $25^{\circ}C$ and $30^{\circ}C$). When the cabbage cultivars were incubated at $25^{\circ}C$, they represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cabbage to F. oxysporum f. sp. conglutinans is to dip the non-cut roots of 14-day-old seedlings in spore suspension of $1{\times}10^7$ conidia/ml for 0.5 hr and to transplant the seedlings to plastic pots with a fertilized soil, and then to cultivate the plants in a growth chamber at $25^{\circ}C$ for 3 weeks to develop Fusarium wilt.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.3 no.4
• /
• pp.228-232
• /
• 1970

From the standpoint of fishery science it is an interesting matter to note that the size of Plecoglessus altivelis population on Cheju Island is smaller than that of the Nakdong River located in the southern part of Korea's mainland. Therefore the author carried out a comparative analysis to determine whether the populations differ because of racial or because of environmental conditions. The morphometric characters of the head length and the body length were used for the analysis of covariance and computed by a standard regression method of testing for the homogeneity of the populations. All samples were collected during 1968 and 1969 in the Milyang Stream of the Nakdong River and the Cheonji Stream of Cheju Island. The results of the analysis of the populations based on the significance test are as follows: 1. Since the F value observed was only 1.023 ($n_1=1,\;n_2=42$) the conclusion was reached that the samples could both belong to the same population. 2. In comparison with the deviations of the regression coefficients between the fish of the Nakdong River and Cherinji Stream, the populations were also highly significant suggesting that thelr belong to the same population. 3. The conclusion is that the fish of Cheju Island became a smaller fish in size due to an environmental condition but not due to racial characteristics.

• Research in Plant Disease
• /
• v.21 no.3
• /
• pp.201-207
• /
• 2015

This study was conducted to establish a simple mass-screening method for resistant melon to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (FOM). Root-dipping inoculation method has been used to investigate resistance of melon plants to Fusarium wilt. However, the inoculation method requires a lot of labor and time because of complicate procedure. To develop a simple screening method on melon Fusarium wilt, occurrence of Fusarium wilt on susceptible and resistant cultivars of melon according to inoculation method including root-dipping, soil-drenching, tip, and scalpel methods was investigated. Scalpel and tip methods showed more clear resistant and susceptible responses in the melon cultivars than root-dipping inoculation method, but tip method represented slightly variable disease severity. In contrast, in the case of soil-drenching inoculation method, disease severity of the susceptible cultivars was very low. Thus we selected scalpel method as inoculation method of a simple screening method for melon Fusarium wilt. By using the scalpel inoculation method, resistance degrees of the cultivars according to incubation temperature after inoculation (25 and $30^{\circ}C$) and inoculum concentration ($1{\times}10^6$ and $1{\times}10^7conidia/ml$) were measured. The resistance or susceptibility of the cultivars was hardly affected by all the tested conditions. To look into the effectiveness of scalpel inoculation methods, resistance of 22 commercial melon cultivars to FOM was compare with root-dipping inoculation method. When the melon cultivars were inoculated by scalpel method, resistance responses of all the tested cultivars were clearly distinguished as by root-dipping method. Taken together, we suggest that an efficient simple mass-screening method for resistant melon plant to Fusarium wilt is to sow the seeds of melon in a pot (70 ml of soil) and to grow the seedlings in a greenhouse ($25{\pm}5^{\circ}C$) for 7 days, to cut the root of seedlings with a scalpel and then pour a 10 ml-aliquot of the spore suspension of $1{\times}10^6conidia/ml$ on soil. The infected plants were cultivated in a growth room at 25 to $30^{\circ}C$ for about 3 weeks with 12-hr light a day.

• Journal of Korean Society for Quality Management
• /
• v.29 no.2
• /
• pp.37-45
• /
• 2001

A survival variable is a non-negative random variable X with distribution function F(t) satisfying F(0) : 0 and a survival function F(t): 1-F(t). This variable is said to be New Better than Used of specified age t$_{0}$ if F(x+ t$_{0}$)$\leq$F(x).F(t$_{0}$) for all x$\geq$0 and a fixed t$_{0}$. We propose the test for H$_{0}$ : F(x+t$_{0}$)=F(x).F(t$_{0}$) for all x$\geq$0 against H$_1$: F(x+t$_{0}$) $\leq$ F(x).F(t$_{0}$) for all x$\geq$0 when the specified age to is unknown but can be estimated from the data when t$_{0}$=ζ$_{p}$, the pth percentile of F. This test statistic, which is based on the normalized spacings between the ordered observations, is readily applied in the case of small sample. Also, our test is more simple than Ahmad's test (1998). Finally, the performance of our test is presented.our test is presented.